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Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Modern Laboratory Medicine is the property of Journal of Modern Laboratory Medicine Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Bacterial vaginosis (BV), an imbalance of normal vaginal microecology, is manifested as a mixed infection. It is characterized by a decrease of normal hydrogen peroxide-producing lactobacillus and an overgrowth of anaerobic bacteria (mobiluncus, prevotella) and facultative bacteria (gardnerella vaginalis, atopo vaginalis). BV is a risk factor for pelvic inflammatory disease, infertility, sexually transmitted pathogens (human papillomavirus, human immunodeficiency virus, neisseria gonorrhoeae, chlamydia trachomatis, etc.) infection. In addition, BV during pregnancy can lead to adverse obstetrical outcomes, including early miscarriage, premature delivery, postpartum endometritis, and low birth weight. The pathogens causing BV are diverse and have a high recurrence rate after treatment. About 10% to 50% of BV patients have no clinical symptoms and are easy to miss diagnosis. Rapid and accurate laboratory detection can provide timely diagnosis and treatment for BV patients and avoid adverse outcomes. Therefore, this paper reviews the research progress of diagnostic criteria, vaginal microecological evaluation and novel detection methods for BV, in order to provide reference for clinical diagnosis and treatment of BV.

ObjectiveTo report rapid pathogen isolation and identification, virulence gene detection and pathogen tracing for a suspected cholera epidemic. MethodsFour stool and four anal swab specimens were collected from 8 diarrhea patients in an overseas tour group and 13 anal swab specimens were also collected from 13 other members of the tour group on October 21, 2018. Rapid detections of the specimens were conducted with Filmarray system and real-time PCR rapid identification and culture methods were used, respectively, for the specimens. VITEK-2 automatic microbial identification instrument and pulsed field gel electrophoresis (PFGE) typing were used for bio-identification of the isolated strains. Virulence genes were analyzed with real-time PCR. ResultsVibrio parahemolyticus (VP) was isolated from 11 of the specimens. Molecular typing of PFGE showed that the 11 VP stains were highly homologous, indicating that the epidemic was resulted from the infection of a same VP strain. ConclusionCombined utilization of multiple laboratory detections is of important value for rapid diagnosis, pathogen identification and tracing in epidemiological investigation on cholera epidemics.

Objective To understand the epidemic situation of brucellosis in these areas, so as to provide a reference for clinical early prevention and diagnosis through the epidemiological analysis of brucellosis in some areas of Yunnan. Methods The epidemiology and clinical manifestations of brucellosis patients treated in Kunming Third People's Hospital from November 2020 to February 2022 were analyzed retrospectively. Results The 175 cases were mainly in Kunming (41.71%)and Qujing (38.86%)；The age was concentrated in 41～60 years old (53.14%), and the ratio of men and women was 2.13∶1. Cattle and sheep breeders were more common (65.71%)；Most of them entered the living environment of cattle and sheep for a long time (99.43%)；The peak incidence was from October to December (36.57%). Fever accounted for 67.43%, night sweat 82.29%, joint pain 85.71% (low back pain 60.00%). 1.14% had no clinical symptoms. Spinal lesions accounted for 90.48% of complications. Test tube agglutination test (SAT) was positive in all patients, and the titer was between 1∶50++～1∶3 200. The positive rate of Brucella Maltese cultured in blood was 39.43% (69 / 175 cases)；The positive rate of bone marrow culture was 38.55% (32 / 83 cases）. Conclusion This group of cases mainly comes from Kunming and Qujing areas, which often occur from October to December. They are mainly infected in the living environment of cattle and sheep. The bone lesions of the 4th and 5th lumbar vertebrae are the most common complications. [ABSTRACT FROM AUTHOR]

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Tropical Medicine is the property of China Tropical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the diagnostic value and correlation of corresponding laboratory indexes in rheumatoid arthritis （RA） complicated with coronary heart disease （CHD） . Methods According to gender and age， patients were divided into RA concurrent CHD group， simple RA group， simple CHD group and healthy control group，with 102 cases in each group. The Level change of RA specificity index （ESR， RF， ACCP， IL- 6） and CHD specificity index （CK，CK- MB，α- HBDH，hs- TnT， Mb） in the four groups were analyzed retrospectively， and we statistically analyzed the correlation between the two groups of specific indicators in RA complicated with CHD group. Re s ults The rate of joint deformity in RA sick group （including simple and concurrent） was significantly higher than that in non- RA group （P＝0.000）， the hypertension prevalence and smoking rate in simple CHD group were significantly higher than the other three groups （P＝0.016，0.021）， there was no significant difference in BMI and TC between the four groups （P＞0.05） In patients with RA complicated with CHD， the RA specificity index of ESR， RF， ACCP and IL- 6， and CKD specificity index of CK， CK- MB， hs- TnT and Mb were significantly increased （P ＜0.05） . ACCP was positively correlated with CK and CK- MB （P ＜0.05），IL- 6 was positively correlated with CK，CK- MB and Mb （P ＜0.05） . Conclusion The detection of ACCP or IL- 6 in RA patients can predict the incidence of RA complicated with CHD. [ABSTRACT FROM AUTHOR]

Objective: We reported a case of a rare type of variable acute promyelocytie leukemia (M3 V) ,reviewed the literatures and summarized the clinical features and diagnostic measures of M3V. Methods: Clinical data carries on the contrast analysis, use diagram, at the same time, through the literature review research m3v characteristics and occurrence, development, diagnosis and treatment. Results: In this case, the bone marrow of the patients is mainly with the original granulocyte, the prominent, and the Auer body, and the immune phenotype is CD2+D117- CD13+D33+ CD34+ Conclusions: This case and most reported M3V typical characteristics are not entirely consistent, reflecting the complexity' of cell morphology' and immunophenotype of the M3V. APL is a dangerous disease, with a characteristic of rapid progression and lngh mortality. The incidence of early bleedinjg and DIC would increase, without timely intervention. It is reasonable to give the necessary' empirical treatment for patients with suspected APL Before final diagnosis is made in the laboratory'. [ABSTRACT FROM AUTHOR]

Objective: To investigate the pathogenesis and clinical diagnosis of fetal/neonatal alloimmune thrombocytopenia (FNAIT) and analyze the laboratory test results and clinical data related to the disease, so as to provide reference for clinical treatment and improvement of prognosis., Methods: The clinical data of six neonatal patients with FNAIT in the Neonatology Department of our hospital from March 2017 to September 2020 were retrospectively analyzed, which included laboratory diagnosis, clinical symptoms, treatment, and prognosis., Results: Among six patients, two cases occurred in the first pregnancy and four cases in the second pregnancy. The platelet count of six cases were decreased at admission or during hospitalization and maternal and neonatal serum autoimmune platelet antibody tests were positive. Five cases were accompanied by different degrees of skin and facial bleeding spots or petechiae and ecchymosis, intracranial hemorrhage. Four cases were treated with immunoglobulin and/or steroid hormone therapy (one of them received cross-matched platelets transfusion), while the symptoms of the other two cases improved spontaneously. Five cases recovered and were discharged from the hospital, while one case had not recovered but the family members requested to be discharged forwardly. Four cases were hospitalized within two weeks, but two cases were hospitalized for more than two weeks due to other diseases or factors (e.g., neonatal sepsis, neonatal enteritis, congenital heart disease, neonatal asphyxia, etc.)., Conclusion: FNAIT is characterized by decreased platelet count, with or without bleeding symptoms, and may occur in the first and following pregnancy. FNAIT can recover spontaneously or have a good prognosis after treatment. However, the complication with other diseases or factors may affect the prognosis.

Drug-induced liver injury (DILI) is one of the common adverse drug reactions in the clinic and is also the main reason for the withdrawal of new drugs from the market. Although the overall incidence rate of DILI is not high; however, it can cause severe adverse outcome and even death, and has become the core cause of acute liver failure in Europe and the United States. In addition, DILI diagnosis is a puzzling problem for clinicians. Drug re-stimulation can be used as the "gold standard" in the diagnosis of DILI, but it may re-induce liver failure, so it cannot be recommended for clinical use. Currently, laboratory diagnostic methods including serum biomarker, genetic testing, scoring scale, and so on are available for DILI.

Objective: To analyze the epidemiological and clinical data of an imported case of visceral leishmaniasis in Henan Province, and explore the method of laboratory diagnosis of kala-azar., Methods: The epidemiological and clinical data of an imported visceral leishmaniasis patient were analyzed. Leishmania donovani bodies in bone marrow smears were observed microscopically. The antibody was detected by using rK39 dipstick test strips. Two pairs of specific primers, K13A-K13B and LITSR-L5.8S, were used to amplify kinetoplast DNA and internal transcribed spacer of rDNA of the parasite, respectively., Results: The patient had been in the epidemic area of visceral leishmaniasis, and had symptoms such as irregular fever, splenomegaly, pancytopenia, and inversed ratio of albumin and globulin. The amastigotes of L. donovani were found in the bone marrow smears, and rK39 test strip was positive, and the PCR products of K13A-K13B and LITSR-L5.8S were 87 bp and 285 bp respectively. The similarities of the two fragment sequences to the corresponding sequences of L. donovani were 94% and 100%, respectively., Conclusions: The case is diagnosed as visceral leishmaniasis according to the epidemiological data, clinical manifestations and laboratory test results of the patient, and the pathogen is L. donovani .

Objective: To identify 10 imported cases of Plasmodium ovale wallikeri infection in Sichuan Province from January to April in 2018, and to analyze the P. ovale wallikeri infection status of 1 079 malaria cases reported in this area from 2013 to 2017., Methods: The 10 cases collected in 2018 were examined by microscopy, RDT, nested PCR and sequencing. Meanwhile, the retrospective detections of P. ovale wallikeri of the 1 079 blood samples from past 5 years were conducted by nested PCR using wallikeri -specific primers., Results: For the 10 cases, the microscopic examinations were all positive for P. ovale , of which 2 were mixed infection with P. falciparum , and the results of RDT were all positive for Plasmodium . Except for 2 cases of P. falciparum infection, the routine nested PCR showed negative results for rest of the samples. The nested PCR for the specific detection of P. ovale wallikeri showed the 10 cases were all positive, and the following sequencing confirmed that the 10 cases were all infected with P. ovale wallikeri . The retrospective tests discovered 2 cases both reported in 2017 and recorded as simple P. falciparum infection tuned out to be mixed infection with P. falciparum and P. ovale wallikeri ., Conclusions: This is the first time that imported P. ovale wallikeri infection is founded in Sichuan Province.