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The experiment aimed to address the issues of poor quality of individual silage made from high-moisture Desmodium intortum and waste resources of pineapple leaves, by exploring the effect of mixed silage of different proportion of pineapple leaves and Desmodium intortum on fermentation quality. The mixing ratios included: 100% Desmodium intortum (CK group), 90% Desmodium intortum and 10% pineapple leaves (10% PL group), 85% Desmodium intortum and 15% pineapple leaves (15% PL group), 80% Desmodium intortum and 20% pineapple leaves (20% PL group), and 75% Desmodium intortum and 25% pineapple leaves (25% PL group). The fermentation quality of silage was analyzed after 60 d. The results showed that the content of WSC and LA in silage were significantly increased with the increase of pineapple leaf proportion (P<0.05). Compared with CK group, the pH value and NH3-N content of silage in all treatment groups were significantly decreased (P<0.05), the crude protein loss rate was reduced, and the production of BA was eliminated. The study indicates that it is more suitable to add about 20% of pineapple leaves and Desmodium intortum mixed silage. [ABSTRACT FROM AUTHOR]

Aim: To investigate the expression of STAT3, WWOX and c-myc in non small cell lung cancer(NSCLC) and their clinical significance, and study their relation ship., Methods: Forty resected tissue samples from patients with NSCLC and twenty normal lung tissues were subjected to immunohistochemical staining(SP) for STAT3, WWOX and c-myc., Results: (1)The positive expression rate of STAT3 and c-myc in NSCLC were 92.5% and 90% respectively, obviously higher than normal lung tissues (20% and 15%, P<0.01); The positive expression rate of WWOX was 30%, apparently lower than normal lung tissues(75%, P<0.01). (2) The expression levels of STAT3 and c-myc were related to pathologic types in NSCLC, which in adenocarcinomas were higher than squmous cell carcinomas (P<0.01), which were regardless with tumor size, histodifferentiation level, clinical stage and lymphatic metastasis; The expression of WWOX was ralated to whether lymph node metastasis in NSCLC(P<0.01).(3) There was a negativie correlation between the expressions of STAT3 and WWOX (r=-0.443, P<0.01); But no relationship was found between STAT3 and c-myc overexpression(r=-0.084) and between WWOX and c-myc overexpression(r=0.115)., Conclusion: STAT3 and c-myc were overexpressed in human NSCLC. It indicated these proteins may make an important role in occurrence and development of lung cancer.The expressions of WWOX in human NSCLC was lower, which non expression was may related to the invision and metastasis of tumor. In the tumorigenesis of NSCLC, there is no relationship between expression of STAT3 and c-myc, They played independent roles in oncogenesis of NSCLC. There was a negativie correlation between the expressions of STAT3 and WWOX, but no relationship was found between WWOX and c-myc.

Objective: To examine the change of Smoothened (Smo) expression in the retinofugal pathway and in the growth cones during the period of embryonic day 13 (E13) to E15., Methods: Smo expression in the chiasm and growth cones was observed by fluorescent immunostaining and retinal explant culture., Results: On E13 and E14, Smo was expressed moderately in the retina and optic disc, and in the corner of the retina, Smo expression was especially dense. On E13, Smo expression was detected in the optic nerves and ventral diencephalon, but only in the superficial region of the optic tract on E14. Smo was also detected in the stem and filopodia of the growth cones in the retinal explant culture during this period., Conclusion: Smo expression changes in different developmental phases, suggesting that Smo might play a role in signal optic axon growth during the development of the retinofugal pathway.