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Objective To investigate the clinical efficacy of esophagogastrostomy using seromuscular double-flap technique following laparoscopy-assisted proximal gastrectomy. Methods The clinical data of one patient with gastrointestinal stromal tumor at the gastric cardia who underwent laparoscopy-assisted proximal gastrectomy in September 2019 was retrospectively analyzed. We reviewed the literature about esophagogastrostomy using seromuscular double-flap technique following proximal gastrectomy and summarized the clinical data. Results This case was a 67-year-old male patient. The patient was diagnosed as gastrointestinal stromal tumor at the gastric cardia by both preoperative imaging and postoperative pathologic examination. Laparoscopy-assisted proximal gastrectomy and esophagogastrostomy using seromuscular double-flap technique was performed. The total operative time and anastomosis time were 280 min and 100 min, respectively. The intraoperative blood loss was 40 mL. The time to the first postoperative flatus was 3 d. The time to the liquid diet was 4 d. The duration of postoperative hospital stay was 8 d. No postoperative short-term complication was found. No reflux esophagitis was shown two months postoperatively with endoscopy. The literature review showed that the occurrence of anastomotic leakage, anastomotic bleeding, anastomotic stenosis, and reflux esophagitis of esophagogastrostomy using seromuscular double-flap technique were 0-4.2%, 0-0.7%, 4.0%-29.1%, and 0-8.3%, respectively. Conclusions Esophagogastrostomy using seromuscular double-flap technique following laparoscopy-assisted proximal gastrectomy was safe and effective in terms of preventing gastroesophageal reflux. It can be applied in highly selected patient with upper-third gastric tumor. High attention is still required to prevent and treat the postoperative anastomotic stenosis.

Aim: To construct the eukaryotic recombinant expression plasmid of pcDNA3.1(+)-PRMT1., Methods: Human PRMT1 cDNA was amplified by reverse transcription polymerase chain reaction (RT-PCR). After digested by BamH I, Hind III and ligation, PRMT1 was inserted into pcDNA3.1(+)eukaryotic expression vector. The positive colonies were screened and identified by PCR and sequencing. pcDNA3.1(+)-PRMT1 plasmid was then transfected into the cultured A549 cell line with Lipofectamine(TM);2000. Realtime-PCR and Western blot were used to detect the mRNA and protein expression of PRMT1 respectively., Results: The PRMT1 cDNA was successfully amplified, and pcDNA3.1(+)-PRMT1 were constructed. The inserted sequence in pcDNA3.1(+)-PRMT1 was the same as the sequence of PRMT1 cDNA published in NCBI GenBank. Further, Realtime PCR and Western blot results validated the recombinant plasmid expressed in A549 cell line efficiently., Conclusion: pcDNA3.1(+)-PRMT1 recombinant was successfully constructed.

Objective: To investigate the long-term effects of iodophor on thyroid function and autoimmunity in females., Methods: 119 medical personnel who have been using iodophor as preoperative skin disinfectant more for than 2 years and 123 medical personnel who have not exposed to iodophor were studied. The urinary iodine, thyroid-stimulating hormone (TSH), free triiodothyronine (FT(3)), free thyroxine (FT(4)), thyroid peroxydase antibody (TPO-Ab) and TG-Ab in serum were measured and the thyroid were examined by B mode ultrasound apparatus., Results: The median urinary iodine (MUI) was 300.4 micro g/L in the exposed group, not significantly higher than that in the non-exposed group (269.1 micro g/L, P > 0.05). The TSH levels of the exposed group and non-exposed group were 1.66 mU/L and 1.62 mU/L (P = 0.84). B mode ultrasound examination showed that the prevalence rate of thyroid disorders was 3.36% in the exposed group, not significantly different from that in the non-exposed group (2.44%). The thyroid autoantibody positive rate was not significantly different between these two groups too. The titer of autoantibody in the exposed group was 29.5 IU/L, significantly higher than that in the non-exposed group (22.4 IU/L, P = 0.048). The titer of TG-Ab in the exposed group was 21.85 IU/L, not significantly different from that in the non-exposed group (18.7 IU/L, P = 0.542). The mean titer of TPO-Ab in exposed group was 29.5 IU/L, significantly higher than that in non-exposed group (22.4 IU/L, P = 0.048). The total prevalence rate of all sorts of thyroid disorders in the exposed group was 11.76%, significantly higher than that in non-exposed group (4.07%, P = 0.026). The thyroid disorders discovered included clinical hyperthyroidism and subclinical hypothyroidism., Conclusion: Using iodophor as preoperative skin disinfectant may result in an increased incidence of thyroid disorders in female medical personnel. Medical personnel with iodophor exposure history should have their thyroid function and thyroid autoimmune status examined regularly.