Search

Two-dimensional nanomaterials are used in biosensors due to their unique physical and chemical properties, which have many advantages in the development of biosensors, such as improving the response speed, improving the detection sensitivity, shortening the detection time and simplifying the operation procedure. In recent years, they have shown great application prospects in the field of food safety detection and have gradually become a hot research topic. Based on an analysis of the application of two-dimensional nanomaterials including graphene, graphene alkyne, and graphene-like materials in the field of analytical sensing, this paper reviews the progress that has been made in the past five year in the application of two-dimensional nanomaterials in the detection of organic pollutants in foods, food additives, foodborne pathogens and heavy metals, and also presents an outlook on future prospects. This review is expected to provide ideas and theoretical references for researchers.

This study aimed to obtain a lentinan-specific fluorescent probe for the in situ visualization of lentinan. A carbohydrate-binding module with potential lentinan-binding capacity was discovered in a β-1,3-D-glucanase by using the bioinformatics techniques, and further recombinantly expressed. The binding specificity of the expressed protein, which was named as LBM was determined by the microtiter plate assays. The results indicated that LBM displayed a desired specificity for lentinan. The protein exhibited positive binding signals to lentinan, while could not bind to several examined polysaccharides including barley β-glucan, curdlan, or konjac glucomannan. Furthermore, the first lentinan-specific fluorescent probe was successfully constructed by fusing LBM with a green fluorescent protein EmGFP, based on which the in situ visualization of lentinan in Lentinula edodes was realized. It was shown that lentinan was not only presented in the mycelial cell wall, but also amorphously distributed in the interstitial space and inside the cell. The construction of the EmGFP-LBM provided a promising tool for the in situ visualization of lentinan. [ABSTRACT FROM AUTHOR]