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【Objective】 Organic fertilizers have been increasingly used as a substitute to reduce the detrimental impact of chemical fertilizers in China. In this paper, we studied the impact of its combination with drip irrigation water temperature on honey jujube growth and yield. 【Method】 The experiment was conducted in a field at Tuha Basin, where 10% to 90% of the chemical fertilizers was replaced by organic fertilizer. The control was without organic fertilizer substitution. For each fertilization, there were two irrigation water temperatures: conventional water temperature (13±1)℃ (T1) and warmed water temperature (21±1)℃ (T2). In each treatment, we measured water consumption, yield and water use efficiency of the jujube. 【Result】 When fertilization was the same, T2 reduced water content in the 0~100 cm soil layer and increased water consumption. Compared with T1, T2 increased yield and water use efficiency of the jujube by 7.15% and 5.10%, respectively. When the irrigation water temperature was the same, substitution of chemical fertilizer with organic fertilizer led to an increase in soil water content and water consumption. As a result, it increased the yield and water use efficiency of the jujube. However, when the substitution rate exceeded 50%, there was a decline in yield and water use efficiency. Specifically, when the substitution was 50%, T2 increased the yield and water use efficiency of the jujube by 10.10% and 6.95%, respectively, compared to T1. 【Conclusion】 Multivariate regression analysis suggested that under conventional irrigation water temperature, substituting 50.00%~55.64% of chemical fertilizer by organic fertilizer is optimal to improve yield and water use efficiency of the jujube, while when increasing the water temperature to 20 ℃, substituting 41.01%~49.83% of chemical fertilizer works the best for the jujube.

As the pathogenic bacterial virulence and avirulence factors, transcription activator like (TAL) effectors of Xanthomonas can resulted in the host diseases or resistance responses. TAL effectors can specifically bind the target DNA of host plant with a novel protein-DNA binding pattern in which two amino acids recognize one nucleotide. The complexities of TAL-DNA binding have the feasibility in use of gene therapy through homologous recombination and site-specific mutation. By using the molecular recognition code between TAL-effectors and host target genes, we can exploit both the susceptible and resistance genes; broad spectrum resistance induced by multiple TAL effectors could also be manipulated. Deeper insight in the area of protein-DNA binding mechanism will benefit the application in the biomedical engineering and agricultural engineering. This article reviews the findings and functions of TAL effectors, the binding specificity and recognition code between TAL-effectors and host target genes. The possible applications and future prospects of the molecular recognition code have been discussed.