Search

In this paper, the water level coseismic response characteristics of Sichuan fluid network to the three iarge earthquakes, the 2022 Lushan MS6. 1, the 2022 Maerkang MS6. 0 and the 2022 Luding MS6. 8 earthquakes, are analyzed statistically. The results show that the three strong earthquakes induced different degrees of water level coseismic response in most areas of Sichuan. The coseismic responses amplitude of water level induced by the three earthquakes are significantly correlated with the earthquake magnitude, while not significantly correlated with well-epicenter distances. In a large range, the coseismic response amplitude of water level are influenced by the aquifer lithology, geological structure, and wellbore conditions. The coseismic response types of water level are mainly affected by the well-epicenter distances, and little related to the wellbore-depth. For the 2022 Luding MS6. 8 earthquake, the reason for the large difference in the coseismic response amplitude of water level of wells Chuan-20, Chuan-49 and Chuan-50 may be the different wellbore bearing capacity. Due to the effects from the aquifer lithology, the location in the fault zone and the wellbore bearing capacity, the coseismic response amplitude of the water level of wells Chuan-46, Chuan-47 and Chuan-48, being close to each other in the same fault zone, are different in the three strong earthquakes. In the 2022 Lushan MS6. 1 earthquake, the coseismic response types of water level between wells Chuan-46 and Chuan-47 are different, which may be related to the different main control factors of dynamic or static stress on the wells water level. The statistical and analytical results of water level coseismic response are affected by the sampling rate of instruments. The analysis results of water level coseismic response, using minute water level data, may not be accurate enough. The seconds water level data can provide more accurate and rich water seismic waves information for the detailed study of the water level coseismic response characteristics and mechanisms. [ABSTRACT FROM AUTHOR]

An approach to registering 6-DOF electromagnetic tracker for stereoscopic virtual environment is proposed. To put tracking sensor with certain posture at a known point in real space, its position and orientation values in screen coordinate system and electromagnetic tracker coordinate system are caught. According to the values, the transformation matrix between these two systems can be calculated. Then the registration is realized, and the outputs of tracker can be transformed into coordinate values in screen coordinate system in real time. Experiments showed that the proposed approach is of low-cost and simple. In addition, the error is less than 10 mm, and meets the requirements of natural manipulation of multi-sensory virtual reality system. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of miR-29b-3p on apoptosis and proliferation of acute myeloid leukemia (AML) cells by targeting signal transducer and activator of transcription 3 (STAT3)., Methods: TargetScan and miRanda online databases were used to predict the binding sites of miR-29b-3p and STAT3 3'UTR. The targeting relationship between them was estimated by Dual-Luciferase reporter assay experiment. After miR-29b-3p over-expression, qPCR and Western blot were used to detect the expression of STAT3 mRNA and proteins, flow cytometry to determine the apoptosis of AML cells, and MTS to detect the changes of cell proliferation in each group., Results: Dual-Luciferase reporter assay confirmed that STAT3 was the target gene of miR-29b-3p. After miR-29b-3p overexpression, the expression of STAT3 mRNA and protein decreased. Compared with the control groups, the proliferation of AML cells in the overexpression group decreased and the apoptosis increased (P<0.05)., Conclusion: MiR-29b-3p can inhibit the proliferation and induce apoptosis of AML cells by down-regulating STAT3.

Objective: To investigate the level of serum microRNA-609 and its clinical prognostic value in patients with thalassemia., Methods: One hundred and twenty-seven patients with thalassemia treated in our hospital from April 2017 to April 2018 were selected, 100 healthy persons were selected as control group. The changes of miR-609 were analyzed by RT-PCR, the relationship between miR-609 and clinical indicators of thalassemia was analyzed, and the prognostic risk factors of thalassemia were evaluated by multivariate logistic regression analysis., Results: The relative expression level of miR-609 in thalassemia patients was 3.17±0.24, which was significantly higher than that in control group (P<0.05). The levels of ALT, Plt and MCH in patients with high expression of miR-609 were significantly higher than those in patients with low expression of miR-609 (P<0.05). The levels of Hb and sICAM-1 in patients with high expression of miR-609 were significantly lower than those in patients with low expression of miR-609 (P<0.05). There was no correlation between the level of miR-609 and the patient's sex, age and AST (P>0.05). The incidence rate of mild anemia in high expression group was significantly lower than that in low expression group (P<0.05). There was no correlation between the level of miR-609 and the incidence rate of moderate anemia (P>0.05). The number of patients with severe anemia in the miR-609 high expression group was higher than that in miR-609 low expression group (P<0.05). The incidence rate of dizziness, fatigue and fever in patients with miR-609 high expression group was significantly higher than those in patients with miR-609 low expression (P<0.05). There was no correlation between the level of miR-609 and the incidence rate of nausea in patients with thalassemia. ROC curve showed that the AUC value of microRNA-609 was 0.862, the sensitivity was 83.6%, and the specificity was 84.1%, which suggested that miR-609 had a high diagnostic value for thalassemia. Multivariate logistic regression analysis showed that MCH and mir-609 were risk factors for poor prognosis of thalassemia patients., Conclusion: The increased level of serum miR-609 in patients with thalassemia is a risk factor for poor prognosis and can be used as a reference index for evaluating the efficacy for patients.

Aim: To reveal the classification of lymphocytes and expression of cytokines infiltrating in HCC, and investigate the significance of changes of immune microenvironment in HCC., Methods: 76 tumor and non-tumor tissues of HCC were collected to detect the amount of immune cells in the tissues by FCM and immunohistochemistry (IHC). Th1/Th2 cytokines in tissues were detected by Cytometric bead array (CBA), and TGF-1, VEGF detected by ELISA., Results: There were more CD3(+); T cells, CD4(+); Th cells, Treg cells, and CD45RO(+); memory T cells in tumors than in non-tumor tissues. On the contrary there were less CD8(+); CTLs in tumors. There was negative correlation between Treg and Th or CTL cells. CD69, which is the early activating factor, expressed less on CD3(+); T cells and NK cells in tumors than non-tumor tissues. Whereas HLA-DR, which is the late activating factor, expressed more on CD3(+); T cells in tumors than non-tumor tissues. More IL-10, TGF-1 and VEGF were secreted in tumors than non-tumor tissues., Conclusion: Along with the decrease of effective immune cells and increase of suppressor immune cells and cytokines, the lymphocytes infiltrating in the tumor were immune incompitence, which contributed to the tumorigenesis.