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[Objective] This study established a method for simultaneous PCR detection of infectious spleen and kidney necrosis virus (ISKNV), Siniperca chuatsi ranairidoviru (SCRIV) and Siniperca chuatsi rhabdovirus (SCRV). [Method] Three pairs of specific primers were designed based on MCP gene in ISKNV, MCP gene in SCRIV and N gene in SCRV, and annealing temperature and primer dosage in PCR amplification reaction were optimized. A multiplex PCR was established to detect ISKNV (550 bp), SCRIV (400 bp) and SCRV (780 bp) simultaneously. The sensitivity and specificity of the method were verified by comparing with single PCR using 22 suspected infected ISKNV, SCRIV and SCRV samples. [Result] The triple PCR method was established successfully and it was able to detect ISKNV, SCRIV and SCRV simultaneously with good specificity and no amplification of common fish viruses such as IPNV, GCRV, KHV, SGIV, NNV, TiLV and SVCV. The method had good sensitivity with lower detection limit of 1 X 10-1 ng/µuL for the three viral DNAs. The testing of 22 clinical samples showed that the established method and single PCR methods were 100% identical. The positive rates of ISKNV, SCRIV, and SCRV were 27%, 41% and 9%. The positive rate of mixed infection of ISKNV and SCRIV was both 9%, while the positive rate of mixed infection of ISKNV, SCRIV and SCRV was 0. [Conclusion] The established multiple PCR detection method had high specificity and sensitivity. Thus, it can be used for rapid identification and diagnosis of the three viruses. [ABSTRACT FROM AUTHOR]

Viruses "hijack" cellular metabolism to complete their proliferation. Glucose is an important source of energy and carbon in the synthesis of precursor molecules in host cells, and its metabolism is regulated dramatically during virus infection. Here, we reviewed the mechanism of virus infection that alters glucose transport, expression of glucose metabolism-related genes (glycolysis, pentose phosphate pathway, gluconeogenesis) in cells, as well as islet cells and insulin receptors. It provides references for study of virus-altering cellular glucose metabolism.

Objective: The aim of this study is to identify strain JX-09 and confirmed that the strain is the pathogen of diseased grass carp (Ctenopharyngodon idellus)., Methods: A pathogenic strain JX-09 was isolated from the diseased grass carp. The strain was identified based on physiological and biochemical characteristics, and the sequence analysis of 16S rRNA. Virulence of strain JX-09 to healthy grass carp was also tested. Furthermore, drug sensitivity was detected, Results: Strain JX-09 was identified as Plesiomonas shigelloides by with Kirby-Bauer's agar diffusion method. biochemical analysis and molecular biology. The P. shigelloides strain was re-isolated from the artificial infected grass carp, and the LD50 was about 6.4 x 10(4) cfu/g. Drug sensitive tests showed that strain JX-09 was susceptible to aztreonam, cefazolin, cephalothin and ceftriaxone, and resistant to kanamycin, medicamycin, vancomycin and piperacillin., Conclusion: Strain JX-09 was the pathogen of grass carp with muscle erosive disease. To the best of our knowledge, this is the first report that P. shigelloides as the pathogenic strain of grass carp.

Objective: To develop a HPLC method for the simultaneous determination of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin in flowers of Pueraria lobata., Method: The separation was performed on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm), using a gradient elution with acetonitrile-water containing 0.2% phosphoric acid as the mobile phase. The flow rate was 1.0 mL x min(-1), the detection wavelength was 265 nm and the temperature of column was 35 degrees C., Result: The linear ranges of tectorigenin-7-O-xylosylglucosid, tectoridin and tectorigenin were 0.14-1.40 (r = 0.9994), 0.20-2.02 (r = 0.9993), and 0. 10-1.04 (r = 0.9991) , respectively. The average recoveries (n=6) of the three constituents were 99.5% (RSD 1.8%), 99.6% (RSD 1.7%), and 99.8% (RSD 1.9%), respectively., Conclusion: The method is simple and accurate with a good eata and can be used as a quality control method for flowers of P. lobata of different sources.