1. [Expression of a single-chain trimer of MHC restricted HBsAg CTL epitope using adenovirus vector containing GFP-report gene].
- Author
-
Chen XC, Liu WL, Yang GL, Liu YJ, Zhu XY, Zhang HM, Zhou BP, and Lybarger L
- Subjects
- Adenoviridae metabolism, Animals, Cell Line, Epitopes, T-Lymphocyte metabolism, Genes, Reporter, Genetic Vectors genetics, Genetic Vectors metabolism, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, H-2 Antigens metabolism, Hepatitis B Surface Antigens metabolism, Histocompatibility Antigen H-2D, Humans, Mice, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Adenoviridae genetics, Epitopes, T-Lymphocyte genetics, Gene Expression, H-2 Antigens genetics, Hepatitis B Surface Antigens genetics
- Abstract
Objective: To generate a recombinant Adenovirus encoding a GFP (green fluorescent protein)-report gene and a single-chain trimer of MHC restricted HBsAg CTL epitope., Methods: An oligonucleotide encoding H-2L(d) restricted HBsAg CTL epitope was synthesized and fused with H-2L(d) DNA molecule to construct the eukaryotic expression vector carrying the HBsAg-SCT gene. The HBsAg-SCT gene was subcloned into a GFP adenovirus expression vector,which was transfected into Ad293 cells for packaging and amplification of recombinant adenovirus encoding HBsAg-SCT., Results: HBsAg-SCT has been cloned into an adenovirus vector encoding GFP report gene successfully as confirmed by double enzyme digestion and direct sequencing. HBsAg-SCT was expressed by infected Ad293 cells demonstrated by western blot assay., Conclusion: A recombinant adenovirus expressing HBsAg-SCT and green fluorescent protein report gene has been generated.
- Published
- 2009