Search

Objective To investigate the effects of Aurora kinase inhibitor MLN8237 on proliferation and apoptosis of human breast cancer cells cultured in vitro. Methods MCF7 cells were divided into two groups，including the control group (without MLN8237) and the observation group ( added with 0. 001, 0.01, 0.1，1，and 10 pmol/LMLN8237) . The cell proliferation inhibitory rate was examined by MTT assay. Cell cycle of MCF7 cells was determined by flow cytometry. The expression levels of phosphorate-Aurora A (p-Aurora A )，apoptosis-related proteins Bcl-2，Bax，and cell cycle-related Cyclin B1 protein were detected by Western blotting. The apoptotic rate was tested by Annexin V-FITC and PI staining. Results MLN8237 (0.01，0.1，1，10 μmol/L ) significantly inhibited the proliferation of MCF7 cells after 24-hour or 48-hour treatment in a dose-dependent and time-dependent manner in the observation group as compared with that of the control group， and the highest inhibition was found at 10 μmol/L MLN8237 after 48-hour treatment (all P <0.05). With the increasing concentrations of MLN8237，the percentage of cells in G2 /M phase increased，the percentage of cells in G0/G1 and S decreased in the observation group as compared with that of the control group， and there was statistically significant difference between these two groups (all P <0.05). Compared with the control group，with the increasing concentrations of MLN8237， the expression of p-Aurora A kinase and Bcl-2 decreased， the expression of Bax increased， the apoptosis rate increased and the highest apoptotic inhibition rate was found at 10 μmol/L MLN8237 after 24-hour treatment in the observation group (all P <0.05). Conclusion MLN8237 inhibits the proliferation and induces the apoptosis of MCF7 cells. [ABSTRACT FROM AUTHOR]