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Objective: To investigate the relationship between serum myeloperoxidase (MPO) level and contrast-induced acute kidney injury (CI-AKI) and prognosis in patients undergoing emergency percutaneous coronary intervention (PCI) and its predictive value.Methods: 381 patients with acute myocardial infarction who underwent emergency PCI in The Second Affiliated Hospital of Anhui Medical University from March 2020 to February 2021 were selected. The clinical data were collected and the serum MPO level was detected within 12 hours before operation. According to the occurrence of CI-AKI after operation, they were divided into CI-AKI group (n=34)and non CI-AKI group (n=347). Multivariate Logistic regression was used to analyze the influencing factors of the incidence of CI-AKI in patients undergoing emergency PCI, and receiver operating characteristic (ROC) curve was used to evaluate the effectiveness of MPO in predicting CI-AKI. According to the best cutoff value, the patients were divided into high MPO group and low MPO group, and the patients were followed up for 12 months. The cumulative incidence of major adverse cardiovascular events (MACE) in high MPO group and low MPO group were analyzed by Kaplan-Meier survival curve. Results: The proportion of combined hypertension, diabetes mellitus, hyperlipidemia and smoking history proportion in CI-AKI group were higher than those in non CI-AKI group, and MPO level, creatinine level, contrast agent dosage were higher than those in non CI-AKI group (P＜0.05). Multivariate Logistic regression analysis showed that diabetes mellitus, high MPO and creatinine level were the risk factors influencing the occurrence of CI-AKI after emergency PCI (P＜0.05). ROC curve analysis showed that the area under the curve (AUC) of preoperative serum MPO level in predicting CI-AKI after emergency PCI was 0.726, and the optimal cut-off value was 491.12 μg/L, with a sensitivity of 58.80%, and specificity of 76.50%. A total of95 cases of MACE occurred one year after operation, with an incidence of 24.93%. Kaplan-Meier survival curve showed that the incidence of MACE in high MPO group was 27.37% higher than that in low MPO group 22.77% (P＜0.05). Conclusion: The serum MPO level of patients with CI-AKI after emergency PCI significantly increased, which is associated with the prognosis of patients, and it is a risk factor for CI-AKI, with high specificity, which can be an effective auxiliary indicator for predicting the occurrence of CI-AKI after emergency PCI. [ABSTRACT FROM AUTHOR]

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Objective: To explore the potential role and mechanism of compound tetramethylpyrazine in gastric cancer therapy by using network pharmacology analysis combined with gene function annotation and clinical data analysis., Methods: SwissTargetPrediction database was used to screen the potential drug action sites of compound tetramethylpyrazine, and the OMIM and Genecard databases were used in combination to obtain gastric cancer-related targets. Intersection analysis was performed to identify potential therapeutic targets. Subsequently, the method of ClusterProfiler was used to perform functional annotation of the downstream targets of intersection. In addition, The Cancer Genome Atlas (TCGA) database was used to obtain the original data of gastric cancer patients, and the immune infiltration analysis, miRNA analysis, transcriptional regulation analysis of key genes, gene set enrichment analysis (GSEA), gene set variation analysis (GSVA), nomogram model construction, and genome-wide association studies (GWAS) were performed., Results: Through network pharmacological screening, we found 14 potential therapeutic targets through which tetramethylpyrazine acted on gastric cancer. Functional annotation showed that these targets were mainly involved in the pathways for hormone metabolism, drug metabolism, and signal transduction. Based on log rank test, the expression of the key genes, ELANE and MPO , showed significant difference in the comparison of gastric cancer survival curves ( P <0.05), and were closely associated with immune cell infiltration. In addition, GSEA and GSVA results suggested that ELANE and MPO might influence the development of gastric cancer through multiple signaling pathways., Conclusion: In this study, by using multiple analysis methods in an integrated way, we found that ligustrazine may have therapeutic effects on gastric cancer by regulating the potential targets of ELANE and MPO , as well as the relevant signaling pathways., Competing Interests: 利益冲突　本文作者姜昊是本刊编委会青年编委。该文在编辑评审过程中所有流程严格按照期刊政策进行，且未经其本人经手处理。除此之外，所有作者均声明不存在利益冲突, (© 2024《四川大学学报（医学版）》编辑部 版权所有Copyright ©2024 Editorial Office of Journal of Sichuan University (Medical Sciences).)

Objective: To investigate the level and clinical significance of Gal-3, MPO, GM-CSF and TGF-beta 1 in bronchoalveolar lavage fluid of children with asthma in different periods. Methods: 114 cases of children with asthma in our hospital from March2016 to June 2017 were(observation group) and 36 cases of non asthmatic children(control group) BALF as research sample, 1 levels of total cell counts and classification as well as Gal-3, MPO, GM-CSF and TGF-β1 were measured and analyzed. Results: Mild and severe HIE group and control group in the bronchoalveolar lavage fluid of TCS macrophages, no significant difference(P>0.05), but in eosinophil and epithelial cell number had significant difference(P<0.05); with statistical significance of neutrophils, moderate and severe group eosinophils and epithelial cell number difference(P<0.05); the observation group with bronchoalveolar lavage fluid Gal-3, MPO,GM-CSF, TGF-beta 1 expression level was higher than the control group [3.59±0.49 vs 10.27±3.89], [13.82±3.54 vs 21.03±7.05],[123.07 ±25.68vs154.82 ±36.44], [121.68 ±7.98vs221.59 ±44.12] in; severe group with bronchoalveolar lavage fluid Gal-3, MPO,GM-CSF, TGF-beta 1 expression level is higher than that of mild group [6.91±1.43 vs 14.42±3.17], [15.52±vs25.18±9.73], [4.42129.53±30.42vs164.78±34.03], [171.35 ±21.48vs240.83±34.08]; there was a positive correlation between the levels of Gal-3 and MPO, GM-CSF and TGF-β1 in bronchoalveolar lavage fluid of asthmatic children in different stages. Conclusion: The changes of the levels of Gal-3, MPO, GM-CSF and TGF-β1 in BALF of asthmatic children are closely related to the condition, which can be used as an effective index to monitor the development of asthma. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effects of 5-ASA-nanoparticles (NP) on TNBS-induced colitis in rats. Methods: Twenty-four BALB/c rats were randomized into normal control group, colitis group, 5-ASA group, and 5-ASA-NP group, with six rats in each group. 5-ASA, 5-ASA-NP and normal saline solution were oral administrated respectively on day 2-7 after the establishment of colitis. On day 8, all animals were sacrificed. The change of body weight, weight to length ratio of colon, and macroscopic and pathologic score of colon were assessed. MPO activity of colonic tissue was measured. Results: Compared with normal group, the DAI, macroscopic and pathologic scores, MPO activity in colonic tissue in TNBS-control group increased significantly. In rats treated with 5-ASA or5-ASA-NP, all the above-mentioned indices decreased significantly, and efficacy of 5-ASA-NP was almost the same as 5-ASA (50mg/Kg). Conclusion: 5-ASA-NP prepared by supercritical dispersion has significant effect on TNBS-induced colitis in rats with lower side effects, which suggested that it might be a potential therapeutic strategy for IBD. [ABSTRACT FROM AUTHOR]

This study deciphered the mechanism of Shenling Baizhu Powder in treatment of mouse model of ulcerative colitis(UC) via NOD-like receptor thermoprotein domain 3(NLRP3) signaling pathway. After three days of adaptive feeding, 70 SPF-grade BALB/c mice were randomized into 7 groups: normal group, model group(dextran sodium sulfate, DSS), mesalazine group(DSS + 5-aminosalicylic acid, 5-ASA), NLRP3 inhibitor group(DSS + MCC950), and high-, medium-, and low-dose Shenling Baizhu Powder groups(DSS + high-, medium-, and low-dose Shenling Baizhu Powder), with 10 mice per group. The normal group had free access to double distilled water, and the remaining groups had free access to DSS-containing water to establish the acute UC model. Intragastric administration was started at the same time as modeling for one week. During the experiment, the general mental state and disease activity of each group of mice were recorded and scored. After the experiment, colon and serum samples were collected. The pathological changes in colon tissue were observed through hematoxylin-eosin(HE) staining. Enzyme-linked immunosorbent assay(ELISA) was employed to determine the levels of interleukin-18(IL-18) and myeloperoxidase(MPO) in colon tissue and interleukin-1β(IL-1β) in serum. Immunofluorescence(IF) and immunohistochemistry(IHC) methods were employed to examine the expression of NLRP3 and IL-18 in colon tissue. Western blot was employed to measure the protein levels of NLRP3, apoptosis-associated speck-like protein(ASC), cystein-aspartate protease 1(caspase-1), and downstream inflammatory cytokines in colon tissue. Compared with the normal group, the modeling of UC increased the disease activity index(DAI), colon pathological injury score, IL-1β level in serum, and IL-18 and MPO levels in colon tissue(P&lt;0.05, P&lt;0.01). Furthermore, the modeling caused obvious pathological changes and up-regulated the expression of NLRP3, caspase-1, ASC, pro-IL-1β, cleaved-IL-1β, pro-IL-18, and cleaved-IL-18 in the colon(P&lt;0.01). Compared with the model group, the administration of corresponding drugs decreased the DAI, pathological injury score, IL-1β level in serum, and IL-18 and MPO levels in colon tissue, and down-regulated the protein levels of NLRP3, caspase-1, ASC, pro-IL-1β, cleaved-IL-1β, pro-IL-18, and cleaved-IL-18 in the colon(P&lt;0.05, P&lt;0.01). According to the results of previous study and this study, we concluded that Shenling Baizhu Powder can alleviate the inflammatory response and intestinal damage of DSS-induced UC by regulating the expression of the proteins and inflammatory cytokines associated with NLRP3 signaling pathway.