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To investigate cytotoxic secondary metabolites of Micrococcus sp. R21, an actinomycete isolated from a deep-sea sediment (-6 310 m; 142 degrees 19. 9' E, 10 degrees 54. 6' N) of the Western Pacific Ocean, column chromatography was introduced over silica gel, ODS, and Sephadex LH-20. As a result, eight compounds were obtained. By mainly detailed analysis of the NMR data, their structures were elucidated as cyclo(4-hydroxy-L-Pro-L-leu) (1), cyclo(L-Pro-L-Gly) (2), cyclo( L-Pro-L-Ala) (3), cyclo( D-Pro-L-Leu) (4), N-β-acetyltryptamine (5), 2-hydroxybenzoic acid (6), and phenylacetic acid (7). Compound 1 exhibited weak cytotoxic activity against RAW264. 7 cells with IC50 value of 9.1 μmol x L(-1).

Objective: This study was designed to find out the changes in microflora on the stress-bearing areas after insertion of removable partial dentures, and in turn to explore the difference between such changes caused by metal bases and acrylic resin bases respectively., Methods: Sixteen patients who were ready to accept removable partial dentures were selected and divided into two groups. Eight patients used metal bases while the other eight patients used acrylic resin bases. Then at the following three points: before dentures were inserted, one month after wearing and three months after wearing, the total cultivable flora were counted and the proportions of bacteria detected on the stress-bearing areas were calculated., Results: No matter what type of bases was selected, the wearing of removable partial denture enhanced the accumulation of microflora on stress-bearing areas. The percentages of Candida albicans and Lactobacilli detected on stress-bearing areas also increased significantly (P < 0.05). When such changes were compared, the authors found that the increment of Candida albicans caused by acrylic resin bases was higher than that caused by metal bases (P < 0.05)., Conclusion: The wearing of removable partial dentures forms new retention areas between the bases and the stress-bearing areas, as a result enhances the deposit of microbe and facilitate the growth of Candida albicans and Lactobacilli. Compared with increment of Candida albicans caused by metal bases, the increment relating to acrylic resin bases is more evident.

In this paper, we concentrated in examining, in vitro, the antiseptic activity of the baicalein and baicalin upon the seventeen pathogenic skin fungal and sixteen skin bacterial strains, these two flavonic compounds were known principally as the biosubstances of a traditional Chinese medicinal plant: Scutellaria baicalensis. In agar media, the baicalein possessed potent specific activity against the pathogenic yeasts with MICs of 70-100 micrograms/ml; But in the same condition, no inhibitory effect was observed upon dermatophytes and filamentous imperfect fungi for baicalein, and upon all used strains for baicalin. According to the antibacterial test of baicalein, a high efficacy was achieved against certain causative specie of axillary and foot's odour such as Micrococcus sedentarius, Staphylococcus epidermidis, S. hominis and C. xerosis with a MICs inferior to 250 micrograms/ml. The good inhibitory activity of baicalein could be linked to the group hydroxyl (-OH) in position seven of the molecule.

Objective: In this article we investigated and analyzed the pathogenetic bacteria of burn infection and their drug resistance in recent 5 years from 1991., Methods: The patients are divided into 2 groups. The 1st group is from June 1986 to May 1991, and the 2nd group is from June 1991 to June 1996. Blood culture and burn wound bacteria culture were done, and bacteria and their drug sensitivity were analyzed., Results: In blood culture, Micrococcus had risen to rank the top (21.6%), while Staphylococcus aureus fell to rank the third. Among these G+ cocci comprised 56.5%, and G- bacilli 30.1%. In burn wound culture, Pseudomonas aeruginosa with rate of 18.1% was still in the 1st place. Enterobacter cloacae was in the 2nd place with the rate of 13.1%. Among these G- bacilli comprised 58.6%, G+ cocci 35.3%. Micrococcus enterobacter cloacae, Pseudomonas maltophilia, fecal Staphylococcus and candida albicans increased remarkably., Conclusion: The changes of pathogenetic bacteria of burn infection and bacteria drug resistance have certain relations with the wide usage of broad spectrum antibiotics such as cefazidime and imipenem.

Bacterial count and species distribution in hospital ward was investigated. It was shown that the in air total bacterial count was maximum in the second season of the year and the number of Streptococcus above the standard was in the first season. Bacteria in the air of the ward by number was in this order: Micrococcus tetragenus, Staphylococcus, Bacillus subtilis, Streptococcus and Corynebacterium. According to the number of bacteria on the equipment and door handle of the ward, the order, was B. subtilis, Pseudomonas and Staphylococcus. Disinfectant solution used for tools and instruments was proved to be effective. The observation is useful to hospital administration.