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In order to improve the corrosion resistance of anticorrosive coatings and expand the application scope of Iranian rock asphalt, solentless Iranian rock asphalt anticorrosive coatings were prepared throuth a two-component process using the matrix asphalt, Iranian rock asphalt and epoxy resin as film - forming materials with the addtion of fillers, various additives and other components. The anti - corrosion properties of such coatings were studied through AC impedance test and acid, alkali and salt corrosion resistance test. Results showed that when the content of Iranian rock asphalt was 15%, the comprehensive properties of modified asphalt were best. The corrosion resistance of the prepared Iranian rock asphalt anticorrosive coating was much better than that of the petroleum asphalt anticorrosive coating. The corrosion resistance time to acid, alkali and salt immersion was prolonged by more than 10 days. The viscosity of Iranian rock asphalt anticorrosive coating was large at room temperature, and good coating effect was achieved when the coating temperature was about 60 ℃.

The scheme,called SDSA（selectively disclosing sensitive attributes）,was proposed to keep confidentiality of sensitive attributes in credential,which had the following characteristics,such as selectively disclosing sensitive attributes in credential,simple key management,no need of the secure third party.The security of SDSA is proved.The application and implementation of SDSA is illuminated through a typical example.

By applying such methods as literature data and text content analysis, by using such software as ROST to collate the online evaluation data of fitness APP user experience, and by analyzing word frequencies, semantic network and emotion, the authors studied fitness APP user experience current conditions and development suggestions. Fitness APP experience current conditions: "exercise", "software" and "fitness" etc constitute the core layer of user word frequencies, word frequencies include purpose type, service type, emotion type etc., basically fitness users accepted fitness APP products; after using the users had positive emotion and negative emotion concurrently, their self expectation's feedback behavior was intense; their demands for function settings, multi-information and emotional social experience were high; there were problems such as user demands being not associated with design, difficulty to balance function contents and demands, disconnection between online and offline fitness social experience, etc. Fitness APP development suggestions: complete multi-information service; improve user "cognition+thinking" experience; develop intelligent fitness social communication; improve user "action+sensation" experience; perfect fitness function development, improve user "association+emotion" experience. [ABSTRACT FROM AUTHOR]

The aim of the research was to investigate the anti- inflammatory mechanism of Pulsatillae Decoction(PD), and the levels of interleukin(IL)-1α, interleukin-6(IL-6)and endothelin-1(ET-1)secreted by cultured rat intestinal microvascular endothelial cells were determined after treatment with its active ingredients, namely anemoside B4, anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. Endothelial cell was challenged with 1 μg·mL-1 lipopolysaccharide(LPS)for 3 h, and then treated respectively with PD at three concentrations(1, 5 and 10 mg·mL-1 )and its seven ingredients at three concentrations(1, 5 and 10 mg·mL-1 ). The cells were in- cubated at 37 °Cin a cell incubator for 21 h. The supernatants were collected and the content of IL-1α, IL-6 and ET-1 analyzed by ELISA kits. The results showed that as compared with LPS control group, the IL-1αcontents were significantly decreased in the high- dose group of PD, the middle-and high-dose groups of anemoside B4, the middle-and high-dose groups of berberine and the high-dose group of palmatine;The IL-6 contents were significantly decreased in middle-and high-dose groups of PD, the high-dose group of anemonin, the middle-and high-dose groups of aesculin and the high-dose group of esculetin;The ET-1 contents were significantly decreased in the middle-dose group of PD, the middle-and high-dose groups of anemonin and the middle-and high-dose groups of esculetin. It suggested that PD may exert beneficial therapeutic actions on systemic inflammatory response syndrome, multiple organ dysfunction syndrome and immune diseases mediated by IL-1α, IL-6 and ET-1. [ABSTRACT FROM AUTHOR]

We reported a case of pulmonary capillary hemangiomatosis (PCH) and introduced its diagnosis, differential diagnosis, pathogenesis and development of treatment based on the review of Dana Point 2008 Classification of Pulmonary Hypertensiona and current literatures. A 43-year-old female presented progressive dyspnea, elevated pulmonary arterial pressures and CT pulmonary angiography (CTPA) imaging of main pulmonary arterial enlargement and wide spread ill-defined centrilobular nodules of ground-glass opacity. Her histologic features were proliferation of capillary channels within alveolar walls as well as muscularization of arterioles and medial hypertrophy of muscular pulmonary arteries. The treatment with diuretics and warfarin was used promptly, but unfortunately was ineffective. The patient died three months after diagnosis. PCH is a very rare vascular disease with poor prognosis. The diagnosis of PCH rests on the integration of clinical and radiographic information with pathologic features, however pathology is the most reliable means. Because clinical symptoms, imaging and histological features of pulmonary veno-occlusive disease (PVOD) and PCH broadly overlap, differential diagnosis should be made carefully. Among the various pathologic features proliferation of capillaries within alveolar walls is the key point for diagnosing PCH, which is also the most critical criteria for differentiating PCH from PVOD. So far the only definitive treatment for PCH is lung transplantation, without which the patient will die several months after diagnosis.

Objective: To investigate the clinical manifestation, diagnosis and treatment of respiratory amyloidosis., Methods: Data of 11 patients with respiratory amyloidosis diagnosed by biopsy in Peking University First Hospital from January 2002 to January 2012 were analyzed, and the related literatures were reviewed., Results: In the last decade, 250 of 389 402 hospitalized patients were pathologically diagnosed as having amyloidosis, and 11 cases were pathologically confirmed to be respiratory amyloidosis. In these 11 patients, 4 cases were with serum amyloid A (AA) amyloidosis and 7 with light-chain (AL) amyloidosis. The main clinical manifestations included hoarseness, cough and dyspnea. In 4 cases with AA type unilateral larynx was involved and there was no recurrence after surgical resection. Of 7 cases with AL type, 2 cases had involvement of bilateral larynxes and both relapsed after surgery. Diffuse involvement of trachea and bronchi was found in 4 cases, and the chest CT scans showed diffuse thickening and local calcification of the airway wall, bronchial stenosis and nodules protruding into the lumen. Bronchoscopy showed airway mucosal hypertrophy, hyperemia, edema and bronchial stenosis. Lung involvement was found in 3 cases, 2 of which presented with diffuse pulmonary interstitial infiltrates, and another case presented with solitary pulmonary mass and extrapulmonary lesions. Of the 7 cases with AL type, 3 cases were treated by chemotherapy and/or radiotherapy, 3 received surgery, 2 underwent autologous hematopoietic stem cell transplantation, and 2 underwent bronchoscopic interventional therapy. Within 3 years of follow-up, 4 patients were alive, 2 dead and 1 lost to follow up., Conclusions: Respiratory amyloidosis, which can be divided into AA and AL types, is clinically rare. Patients with AA type usually present with local lesions, which can be cured by surgery, while patients with AL type often present with diffuse lesions and require integrated therapies including surgery, interventional treatment, chemotherapy, radiotherapy, and autologous hematopoietic stem cell transplantation.

Objective: To study the chemical constituents of flower buds of Lonicera similes., Methods: The chemical constituents were isolated and purified with macroporous resin, silica gel column chromatography, gel chromatography, ect. The structures were elucidated according to the references combined with the application of spectrum techniques such as 1H-NMR, 13C-NMR ect., Results: Nine compounds were isolated and their structures were identified as 5-hydroxyl-7,4'-dimethoxyflavone (I), rutin (II), quercetin (III), amentoflavone (IV), aesculetin (V), beta-sitosterol (VI), 5-hydroxyl-7,3', 4'-trimethoxyflavone (VIII), oleanolic acid (VIII), beta-daucosterin (IX)., Conclusion: Among the nine isolated compounds, compounds I, II, III, IV, V, are flavonoids. Compounds I , IV, V, VII, VIII, IX are isolated from this plant for the first time.

Objective: To elucidate the relationship between the radiological features and prognoses of pneumocystis pneumonia (PCP) in non-acquired immunodeficiency syndrome (non-AIDS) immunocompromised patients., Methods: Retrospective analyses were performed for the radiological features and prognoses of 36 non-AIDS immunocompromised PCP patients hospitalized at Peking University First Hospital from April 2006 to April 2010. There were 24 males and 12 females with an average age of (51.7 ± 18.9) years., Results: All patients underwent chest photographic examinations and chest computed tomography (CT) was performed on 29 of them. The overall case fatality rate (CFR) of 36 patients was 33.3% (12/36). At the start of anti-PCP treatment, there were normal chest radiography (n = 7), bilateral diffuse infiltration (n = 21), bilateral diffuse consolidation (n = 8), aerothorax, mediastinal emphysema or pneumohypoderma (n = 6) and numbers of deceased patients (n = 0, 8, 5 and 5 respectively). The CFRs of the patients with normal chest radiography and aerothorax, mediastinal emphysema or pneumohypoderma were both statistically significant as compared with the overall rate (P < 0.05). At the start of anti-PCP treatment, there were bilateral diffuse ground glass opacities (GGO) (n = 29), "mosaic sign" (n = 19), air bronchogram (n = 19), pulmonary consolidation (n = 17), bilateral pleural effusion (n = 7), thickened lobular septa (n = 6), cystic lesions (n = 5), aerothorax, mediastinal emphysema or pneumohypoderma (n = 4) and numbers of deceased patients (n = 8, 5, 5, 5, 2, 2, 2 and 4 respectively). The CFRs of the patients with aerothorax, mediastinal emphysema or pneumohypoderma were statistically significant as compared with the overall rate (P < 0.05)., Conclusions: The major radiological features of PCP in non-AIDS immunocompromised patients are bilateral diffuse infiltration. And bilateral diffuse GGO on chest CT scans are usually accompanied with "mosaic" sign, air bronchogram or pulmonary consolidation. The prognoses are excellent for the patients with normal chest radiograph and poor for those with aerothorax, mediastinal emphysema or pneumohypoderma.

Objective: A comparative dose distribution study has been undertaken between proton beam therapy (PBT), 3-dimensional conformal radiation therapy (3D-CRT) and intensity-modulated radiation therapy (IMRT) in the treatment of hepatocellular carcinoma (HCC), so as to assess the potential advantages of PBT., Methods: Dose volume histograms (DVHs) were compared between PBT and 3D-CRT or IMRT planning at total dose of 66 Gy and 86 Gy in stage I patients (n = 10, diameter < or = 5 cm), 60 Gy and 72 Gy in stage IIA patients (n = 12, diameter = 5.1-10 cm)., Results: For patients with stage I, the mean liver dose (Dmean), V10, V20 and V30 were 13.01 Gy, 51.89%, 36.13% and 21.24% for 3D-CRT, whereas they were 6.34 Gy, 30.23%, 17.86% and 10.66%, respectively, for PBT (P < 0.002). With dose escalation to 86 Gy, the Dmean, V10, V20 and V30 were 16.91 Gy, 67.51%, 46.84% and 27.61% for 3D-CRT, whereas they were 8.26 Gy, 39.31%, 23.22% and 13.86%, respectively, for PBT (P < 0.002). Compared with 3D-CRT with dose of 66 Gy, PBT reduced the Dmean, V10, V20 and V30 even with dose escalation to 86 Gy (P < 0.042). For patients with stage IIA, the Dmean, V10, V20 and V30 were 29.18 Gy, 72.25%, 58.17%, 44.01% and 24.92 Gy, 73.32%, 56.15%, 37.75% for 3D-CRT and IMRT, respectively, with dose of 60 Gy, whereas they were 16.28 Gy, 43.93%, 33.54% and 22.78%, respectively, for PBT (P < 0.002). With dose escalation to 72 Gy, the Dmean, V10, V20, V30 were 35.02 Gy, 86.70%, 69.80%, 52.81% and 29.90 Gy, 87.98%, 67.74% and 45.30% for 3D-CRT and IMRT, respectively, whereas they were 19.54 Gy, 52.72%, 40.25% and 27.34%, respectively, for PBT (P < 0.002). Compared with 3D-CRT and IMRT with total dose of 60 Gy, PBT reduced the Dmean, V10, V20 and V30 even with dose escalation to 72 Gy (P < 0.05). In all of the 22 cases, compared with 3D-CRT, PBT reduced the doses to the nonliver OARs (organs at risks) including spinal cord, right kidney and stomach (P < 0.002). Compared with IMRT, PBT also reduced the dose to the right kidney and stomach significantly, while no significant difference was found respect to the dose to spinal cord (P > 0.05)., Conclusion: Compared with 3D-CRT, PBT reduced the dose to the normal liver tissues and nonliver OARs significantly, even with 20 to 30.3 percent of dose escalation. Compared with IMRT, PBT reduced the dose to the normal liver tissues significantly, even with 20 to 30.3 percent of dose escalation. PBT reduced the dose to the right kidney and stomach significantly. No significant difference was observed respect to the dose to spinal cord.

Objective: To observe the effect of Wenyang Yiqi Pingchuan Recipe (WYPR) on the pathomorphology of lung and its regulation on lung tissue contents of nitric oxide (NO) and endothelin-1 (ET-1) in rats with bronchial asthma., Methods: Sixty SD rats were randomly divided into 6 groups: the normal group, the model group, and the four treated groups treated with high dose WYPR, low dose WYPR, Guilong Kechuanning Capsule and aminophylline, respectively, 10 in each group. Except those in the normal group, all rats of bronchial asthma model were established by egg protein sensitization and provocated by inhalation. The treatments were given via gastrogavage every day starting from the first provocation (the 3rd week of modeling) to the execution. Rats were sacrificed after 4-week treatment, their lung was taken for determining the contents of NO and ET-1, and histopathological changes in lung were observed as well., Results: Compared with the normal group, the contents of NO and ET-1 in the lung tissue, the thickness of bronchus wall and bronchus smooth muscle, the number of eosinophil granulocytes increased in the model group and the low dose WYPR group, showing statistical significance (P < 0. 01). As compared with the model group, all the above-mentioned indices were lower in all the 4 treated groups (P < 0.05 or P < 0.01), but the lowering in the WYPR treated groups (either high or low dose) was more significant than in the Guilong Kechuanning treated group (P < 0.05 or P < 0.01); while compared with the aminophylline treated group, the high dose WYPR group was superior in reducing eosinophile granulocytes (P < 0.01), but no significance between them was shown in NO and ET-1 levels (P > 0.05)., Conclusions: WYPR could reduce the eosinophilic infiltration, decrease the contents of NO and ET-1 in the lung tissue, restrain the air passage inflammation and inhibit the pathological process as airway remodeling.

Objective: To evaluate the efficacy and safety of talc poudrage pleurodesis via semi-rigid medical thoracoscopy in the treatment of malignant pleural effusions, as well as the factors that may influence the outcomes., Methods: A series of 27 patients with malignant pleural effusion underwent medical thoracoscopic talc poudrage pleurodesis between July 2005 and September 2007 in Peking University First Hospital., Results: There were 16 male and 11 female patients in the series, the average age being 65.2 years. All the patients had documented malignant pleural effusions, including 16 cases of adenocarcinoma, 6 of malignant mesothelioma, 2 of squamous cell carcinoma, 1 of lymphoepithelioma-like carcinoma, 1 of small cell carcinoma and 1 of undifferentiated lung cancer. Thirty days after the procedures, complete successful pleurodesis was achieved in 22 cases, and partial successful in 4 cases. Pleurodesis was not successful in one case. Overall successful rate was 96.3% (26/27). The average duration of thoracic tubing was 6.85 days. Chest pain, fever and an increase in peripheral WBC after the procedure occurred in 19(70.4%, 19/27), 21(77.8%, 21/27), and 12(44.4%, 12/27) cases respectively. No respiratory failure occurred., Conclusion: Medical thoracoscopic talc poudrage pleurodesis is a safe and effective method for the treatment of malignant pleural effusion.

Objective: To describe the clinical, radiological and pathological characteristics of idiopathic pulmonary haemosiderosis (IPH) in adults and to evaluate the methods of diagnosis and treatment., Methods: Two patients were successfully diagnosed and treated in our hospital and the literature on the subject was reviewed., Results: Two adult patients (19 and 34 years old) diagnosed in our hospital had 5 and 10 years of history of hemoptysis respectively, and chest CT showed bilateral diffuse alveolar opacities over mid and lower zones. Tests of antinuclear antibodies (ANAs), rheumatoid factor (RF), antineutrophilic cytopasmic antibodies (ANCA) and Anti-glomerular basement membrane (anti-GBM) antibody were negative. Haemosiderin-laden macrophages were found in bronchoalveolar lavage fluid (BALF) whose color was yellow. Microscopic examination of the lung tissue specimens obtained by transbronchial lung biopsy (TBLB) revealed hemorrhage and numerous hemosiderin-laden macrophages in the alveoli and no vasculitis or capillaritis were seen. These findings were consistent with a diagnosis of IPH. Steroid therapy had good effects., Conclusion: IPH is a diagnosis of exclusion of other causes of diffuse alveolar hemorrhage (DAH). IPH adults have relatively good drug responses and relatively good prognoses.

To describe the clinical, radiological and pathological characteristics of idiopathic pulmonary alveolar proteinosis (I-PAP) and to evaluate the methods of diagnosis and treatment. Three patients were successfully diagnosed and treated in our hospital and the literature on the subject was reviewed. Three patients, two males and one female (mean age 46 years), were diagnosed averagely in 4 months. Two severe patients presented with progressive dyspnea and type I respiratory failure, and one mild patient only with dry cough and hypoxemia. Chest X-ray radiographs all showed perihilar "butterfly" shadow and chest CT scans showed diffused ground-glass opacities (GGO), typically with "map" changes and "crazy paving" patterns. All the patients underwent bronchoscope, branchoalveolar lavage fluid (BALF) had grossly opaque and/or milky appearance and its sediment was periodic acid-Schiff stain positive. Trans-bronchoscopic lung biopsy (TBLB) specimens were obtained and under light microscopy alveoli and some of the small bronchioles were filled with eosinophilic proteinaceous material with needle-like clefts. By electron microscopy numerous cellular debris and extracellular multilamellated bodies were found. Two severe patients were successfully treated with sequential whole-lung lavage and one required repeated lavages. I-PAP is rare and prone to be misdiagnosed. The radiological features may indicate the diagnosis and examinations of TBLB and BALF can make the accurate diagnosis. Whole-lung lavage is the most effective therapy by now and granulocyte-macrophage colony-stimulating factor (GM-CSF) may be beneficial in some patients.

Objective: To evaluate the diagnostic value of serum cryptococcal capsular polysaccharide antigen latex agglutination test (LA) for pulmonary cryptococcosis., Methods: Serum and lung biopsy specimens of 27 patients, who were suspected of having pulmonary cryptococcosis based on clinical presentations and imaging features, were collected from July 2000 to July 2007 in the First Hospital of Peking University. LA test and histopathological examination were performed in all the patients., Results: Histopathological examination confirmed pulmonary cryptococcosis in 9 cases, all of whom were positive for serum LA test. The other 18 cases were confirmed to have no cryptococcosis and all of them were negative for serum LA test. Therefore, the sensitivity and specificity of LA test were both 100%., Conclusion: The diagnostic value of serum LA test for pulmonary cryptococcosis is high.

To investigate the clinical characteristics, diagnosis and therapy of influenza pneumonia with staphylococcal infection. One patient in our hospital was diagnosed and the literatures on the subject were reviewed. The patient presented with high fever and dyspnea. Arterial gas analysis indicated type 1 respiratory failure. Chest X ray photographs showed bilateral infiltrations and bilateral encapsulated pleural effusions. Viral separation and culture of pharyngeal swab indicated H(3)N(2) subtype of human influenza virus. Blood, sputum and bronchoalveolar lavage fluid (BALF) cultures showed Staphylococcus aureus. Pleural effusion was complex parapneumonic pleural effusion. After the administration of anti-virus, anti-staphylococcal antibiotics and pleural cavity drainage, the patient was cured. The infection of staphylococcus aureus is a typical characteristic of influenza pneumonia, and anti-staphylococcal antibiotic therapy (with MRSA activity in MRSA endemic regions) should be initiated in hospitalized cases of influenza pneumonia. If complex parapneumonic pleural effusion or empyema complicated, we should perform pleural cavity drainage in time. The oral neuraminidase inhibitor (oseltamivir) could significantly improve prognosis.

Objective: To describe the clinical characteristics of pulmonary mucormycosis and to evaluate the approaches to its diagnosis and treatment., Methods: Three cases of pulmonary mucormycosis were successfully diagnosed by histopathologic examination of infected tissues obtained by fiberoptic bronchoscopy in this hospital. The clinical features of the cases were analyzed and the literature reviewed., Results: Three patients were all farmers and two of them had had diabetic ketoacidosis. The first patient died from massive hemoptysis one day after the diagnosis was confirmed. The second patient was cured by large doses of intravenous amphotericin B (total dosage 2 g). The third patient presented with right middle bronchial stenosis after right upper lobectomy because of squamous carcinoma, and was cured by amphotericin B (1.5 g) combined with interventional therapy under fiberoptic bronchoscopy. The two patients were followed for 0.5 - 1 year without relapse., Conclusions: The mortality of pulmonary mucormycosis is high. Fiberoptic bronchoscopy is a useful diagnostic method for histopathologic examination. Successful treatment of pulmonary mucormycosis includes early diagnosis, control of underlying diseases and surgical intervention of isolated lesions combined with large dosage of amphotericin B.

Objective: To investigate the regulation of tissue factor (TF) on doxorubicin-induced apoptosis in human neuroblastoma., Method: The expression of TF was examined by Western blotting. TF siRNA-pSUPER plasmid was constructed by inserting a specific 19-nt silencing sequence targeting TF gene into pSUPER vector. Transfection of TF siRNA-pSUPER was performed using lipofectamine 2000. The activation of caspase-3 and PARP induced by doxorubicin was tested by Western blotting. The apoptotic cells were stained by Hochest 33342 and counted under fluorescence inverted microscope., Results: (1) Human neuroblastoma cell line SK-N-MC expressed high level of TF. (2) Downregulation of TF expression was achieved by transfection of TF siRNA-pSUPER into SK-N-MC cells in a dose-dependent manner. (3) Cleavage of caspase-3 and PARP was increased in transfected SK-N-MC cell with down-regulation of TF. (4) TF siRNA treatment at 1 microg/ml for 8 h significantly increased apoptotic cell number in transfected SK-N-MC cells compared to that in non-transfected cells (P < 0.05) while exposing to 1 microg/ml doxorubicin for 8 h., Conclusions: Downregulation of TF expression by specific siRNA vector could increase the cytotoxicity of doxorubicin and enhance doxorubicin-induced apoptosis in human neuroblastoma cells.

Marginal zone B-cell lymphoma of the pulmonary mucosa-associated lymphoid tissue type (pulmonary MALT-MZL), a common kind of primary pulmonary lymphoma, is rare in pulmonary malignant tumors. One patient in our hospital was diagnosed by bronchoscope and the literatures on the subject were reviewed. The patient presented with periodical fever, cough and chest pain, and antibiotic therapy had no use. Chest CT scan showed the consolidation of right middle lobe and left lower lobe with CT angiogram signs, air bronchograms and distended bronchi. Pleural effusion in the left thorax mainly consisted of monocytes. Monoclonal protein was found in the electrophoresis of serum protein. Bronchial stenosis and swollen mucosa were seen with bronchoscope. The tissue section of transbronchial lung biopsy (TBLB) specimens showed diffusedly infiltrated small lymphocytes and a lymphoepithelial lesion. CD20 was positive and CD3, CD5, CD10, CD21, CD23, bcl2, bcl6 were negative in immunohistochemical stain. The clinical manifestations of pulmonary MALT-MZL are nonspecific and misdiagnosis is common, Appropriate invasive biopsy procedures are necessary for early diagnosis. Presentations such as periodical fever, distended bronchi in pulmonary consolidation, monoclonal protein might indicate diagnosis. Treatment includes surgical resection, radiotherapy and chemotherapy. Pulmonary MALT-MZL belongs to inert lymphoma and prognosis is relatively good.

Objective: To observe the effect of Zhongfengkang on the content of insulin-like growth factor-1 (IGF-1) in brain of focal cerebral ischemia rats., Method: The model of focal cerebral ischemia was made by thread-blocking method. In comparison with the curative effect of buchangnaoxintong, the action of Zhongfengkang on infarct volume and the content of IGF-1 in brain of focal cerebral ischemia rats were observed in 6, 12, 24 h and 48 h after cerebral ischemia., Result: As compared with sham operation group, the content of IGF-1 in brain of model group was gradually increased from 6th hour after cerebral ischemia, reaching to the maximal level at 24th hour after the onset of cerebral ischemia (P < 0.01). In comparison with the values in model group at the corresponding time points, the content of IGF-1 in brain of each treatment groups was increased in different extent with a maximal enhancement in the group treated by the high dose of Zhongfengkang (P < 0. 05 or P < 0.01); while the infarct volume of each treatment groups were decreased significantly (P < 0.05 or P < 0.01)., Conclusion: Zhongfengkang could effectively protect the cerebral tissue from injury of focal cerebral ischemia in the rats through increasing the cerebral content of IGF-1 and reducing infarct volume.

Objective: To observe effect of electroacupuncture at "Zusanli" (ST 36) on expression of interferon-gamma (IFN-gamma)-like immunoreactive substance in spinal cord of the rat and to probe the mechanism., Methods: The IFN-gamma-like immunoreactive positive cell number in spinal cord of the rat was investigated with immunohistochemical SP method and microscopy., Results: After electroacupuncture at "Zusanli" (ST 36), IFN-gamma-like immunoreactive positive cell number in the spinal cord of the rat with electroacupuncture plus immunosuppression did not significantly change (P > 0.05). The number of positive cells in the dorsal horn in the rats with immunosuppression was significantly less than that in the normal control group (P < 0.05) with no significant change in other parts and with no significant difference between the electroacupuncture group and the control group (P > 0.05)., Conclusion: Electroacupuncture at "Zusanli" (ST 36) can increase expression of IFN-gamma-like immunoreactive substance in spinal cord of the rat, and acupuncture activates the nerve-immunoregulative network possibly by IFN-gamma as medium.

Objective: To observe the effect of Zhitiaokang capsule (ZTK) on serum insulin-like growth factor-2 (IGF-2) in patients with hyperlipidemia (HLP)., Methods: Patients with HLP were randomly divided into the treated group (n = 124) and the control group (n = 62), they were treated by ZTK and Zhibituo tablet respectively for 8 weeks as one therapeutic course. The changes of blood lipids and serum IGF-2 before and after treatment were observed., Results: ZTK could significantly reduce blood lipids and increase serum content of IGF-2., Conclusion: ZTK could improve the insulin resistant status and inhibit the development of hyperlipidemia in patients by means of raising serum content of IGF-2 and suppressing vascular endothelial cell apoptosis.

Objective: MDM2 is considered a proto-oncogene due to its ability to inhibit P53 tumor-suppressor function. But, evidence showed that MDM2 might have a P53-independent role in tumorigenesis. MDM2 is over-expressed in human sarcoma and carcinoma. Recent studies showed that MDM2 might act as a transcriptional factor to modulate expressions of other genes involved in cell cycle regulation and transformation. In the present study, the investigators hypothesized that MDM2 directly affected NF-kappaB expression and function in a P53-independent manner., Methods: MDM2 was transfected to acute lymphoblastic leukemia (ALL) line EU-4 cells lacking P53 expression and expressing very low levels of MDM2. MDM2 and P65 expression in mRNA level and protein level were detected by Western blot and Northern blot after transfection. Since the expression of E-selectin is P65 dependent, E-selectin promoter-CAT construct and P65 and MDM2 expression plasmids were co-transfected to EU-4 cells. CAT activation was determined with ELISA. The effect of adriamycin (ADM) at the concentrations of 15 micro g/ml, 7.5 micro g/ml, 5 micro g/ml and 1 micro g/ml on MDM2-transfected EU-4 cells and the parent cells was detected by MTT assay., Results: The results showed that MDM2 up-regulated P65 expression at both mRNA and protein levels, and MDM2 increased P65-mediated transactivation of E-selectin promoter. Without P65, MDM2 had no effect on the transactivation of E-selectin. Moreover, MDM2 antisense could not change the transactivation of E-selectin. MTT results showed that the survival rate of MDM2 transfected EU-4 cells was higher than that of parental cells. The results suggested that MDM2 transfection increase drug resistance of EU-4 cells to ADM compared with parent cells., Conclusion: (1) MDM2 up-regulated transcriptionally P65 expression. (2) MDM2 increased drug resistance of leukemia cells to ADM. (3) MDM2 elevated NF-kappaB activity in a P53-independent manner in childhood lymphoblastic leukemia cell line.