1. [Assessment of the Escherichia coli Tat protein translocation system with fluorescent proteins].
- Author
-
Zhang M, Pan RR, Yu ZL, and Wu LF
- Subjects
- Green Fluorescent Proteins, Luminescent Proteins metabolism, Oxidoreductases, N-Demethylating physiology, Protein Folding, Protein Sorting Signals, Escherichia coli metabolism, Escherichia coli Proteins physiology, Membrane Transport Proteins physiology
- Abstract
The possibility of using fluorescent proteins as probes to study the twin-arginine translocation (Tat) system was assessed in Escherichia coli. When fused to the twin-arginine signal peptide of trimethylamine N-oxide reductase, the DsRed2 red fluorescent protein from the Discosoma sp. was successfully synthesized and folded in E. coli cells. However, RR-DsRed2 aggregated inside the cells. Therefore, although DsRed2 has been engineered from DsRed for faster maturation and lower non-specific aggregation, it is still not compatible with Tat-dependent translocation. In contrast, the jellyfish green fluorescent protein (GFP) was efficiently exported into periplasm even when the RR motif was changed to KR or RK. These results show that GFP can be used as an efficient reporter protein to study Tat system, but DsRed2 is not suitable for such purpose because of its aggregation property. In addition, when the protein concentration was similar, the fluorescence intensity of KR-GFP and RK-GFP decreased compared with RR-GFP, which would suggest that the twin-arginine signal peptide is not only essential for mediating protein translocation, but also important for the folding of down-stream protein.
- Published
- 2003