1. [Interleukin-21 surface-modified MB49 cell vaccine for treatment of metastatic bladder in mice].
- Author
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Peng J, Shi X, Chen D, Liang Z, Lan K, Gao J, and Tan W
- Subjects
- Amino Acid Motifs, Animals, Cell Line, Tumor, Female, Immunotherapy, Lymphocyte Activation, Mice, Mice, Inbred C57BL, Neoplasm Metastasis, Cancer Vaccines therapeutic use, Interleukins immunology, Neoplasms, Experimental therapy, T-Lymphocytes, Cytotoxic immunology, Urinary Bladder Neoplasms therapy
- Abstract
Objective: To develop a novel vaccine by immobilizing interleukin-21 (IL-21) on the surface of MB49 cells and evaluate its effect in inducing specific cytotoxic T lymphocytes (CTLs) and antitumor immunity in a mouse model of subcutaneous metastatic bladder cancer., Methods: SA-IL-21 was immobilized on the surface of 30% ethanol-fixed MB49 cells to prepare the cell vaccine. C57BL/6 mice with subcutaneous implantation of MB49 bladder cancer cells were randomized into 5 groups to receive treatments with IL-21/MB49 vaccine, soluble IL-21, GFP surface-modified MB49 cells, ethanol-fixed MB49 cells, or PBS. The tumor growth and CTL were examined to assess the antitumor efficacy of the vaccine., Results: IL-21 surface-modified MB49 cell vaccine significantly inhibited the tumor growth and generated a long-lasting memory response (P<0.05). At the same effector-target (E:T) ratio, the specific CTLs induced by IL-21/MB49 vaccine showed the most potent cytotoxicity against MB49 cells (P<0.05)., Conclusion: With the protein-anchor technique, IL-21 can be efficiently immobilized on the surface of MB49 cells to prepare IL-21/MB49 cells vaccine. The novel vaccine can maintain its biological activity and significantly enhance the cytotoxicity of CTLs against bladder cancer cells.
- Published
- 2012