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Objective To evaluate the risk of hemorrhagic complication after renal biopsy in patients with prolonged APTT and normal thromboelastography (TEG). Methods A total of 327 patients underwent ultrasound-guided percutaneous renal biopsy from January 2021 to July 2022. According to whether or not APTT was prolonged, they were divided into two groups of normal APTT (n=272) and prolonged APTT and normal TEG (n=55). Overt hemorrhagic complications were compared between two groups and subgroups (APTT prolonged 5 s). Also TEG parameters and overt hemorrhagic complication of patients with TEG data in two groups were compared and the correlation was examined between TEG and coagulation. Results No significant inter-group difference existed in baseline characteristics (P>0.05). The incidence of overt hemorrhagic complication was 3.68% in normal APTT group and 5.45% in prolonged APTT and normal TEG group. No significant inter-group difference existed in the incidence of overt hemorrhagic complication (P>0.05). The risk of hemorrhagic complication was not elevated in prolonged APTT and normal TEG group. Furthermore, no significant difference existed in the incidence of overt hemorrhagic complication between subgroups of APTT prolonged 5 s. Analysis of patients with TEG data revealed no significant inter-group differences in TEG parameters or the incidence of overt hemorrhage. Also weak correlation existed between reaction time and APTT. Conclusions APTT can not accurately predict the hemorrhagic risk after biopsy. And renal biopsy is feasible for patients with prolonged APTT and normal TEG.

Ten samples of Cabernet Sauvignon from the northern foot of the Tianshan Mountains in Xinjiang were selected from the end of the alcoholic fermentation to the final color decline (deep purplish red to yellowish brown). The basic physical and chemical indexes, CIELab parameters, polyphenol content, total pigment, polymeric pigment, and other indexes of wine were determined. Pearson correlation analyses were performed on them. Screening of samples for characteristic indicators based on the presence of correlation using partial least squares - discriminant analysis. Ultimately, the visual representation is used to visualize the color of the wine and the relative content of the characteristics. The results showed a correlation between basic physicochemical indicators, polyphenol content, and CIELab parameters. Partial least squares-discriminant analysis, CIELab color space characterization, and two-dimensional greyscale maps showed that the main characteristic indicators of the wine body when it was purplish-red in color were L* (57.97 - 81.21), tannin content (4 322.66 - 4 385.43 mg/L). When the wine was carmine red in color, the main characteristic indicators of the wine were a* (14.69 - 16.41), total phenol content (2 157.59 - 2 983.46 mg/L), and tannin content (3 302.57 - 3 459.51 mg/L). When the wine was ruby red in color, the main characteristic indicators of the wine were a* (18.13 - 20.52), malic acid content (1.10 - 1.40 g/L), and tannin content (4 558.06 - 4 652.22 mg/L). When the wine showed a brick red color, the main characteristic indexes of the wine were tannin content (4 762.08 - 5 185.81 mg/L) and total phenol content (3 568.87 - 3 809.33 mg/L). When the total phenol content was below 2 000 mg/L, the wine was severely yellowed and no longer suitable for aging. This method directly reflects the color and characteristic index information of Cabernet Sauvignon wines with different colors, and can well identify and distinguish Cabernet Sauvignon wines with different colors. It lays a foundation for the construction of a different color Cabernet Sauvignon wine evaluation system in the northern foot of the Tianshan Mountains in Xinjiang. [ABSTRACT FROM AUTHOR]

Synthesizing or retrieving the radial velocity of weather radar can obtain a three-dimensional wind field, which is an important research direction in radar meteorology. A fine three-dimensional wind field helps to study the structure and motion characteristics of small-scale and meso-scale weather systems. Array weather radar (AWR) consists of three-phased array transmit-receive subarrays (referred as transceiver subarrays), which is used for synchronous detection. AWR data are of high temporal and spatial resolution, thus ensuring the correctness of wind field synthesis and retrieval.According to domestic and aboard research, three-dimensional variational data assimilation (3DVAR) wind field retrieval algorithm is quite mature. Using AWR data of 10 rainfall cases at Changsha airport from April to September in 2019, the wind field is retrieved and evaluated. In the three-dimensional fine detection area of the AWR, detection data of a L-band boundary layer wind profile radar and the AWR synthetic wind field are used as reference value to evaluate the retrieved wind field.Results show that the retrieved wind field, the synthetic wind field, and wind profile radar product are more consistent and reasonable in stable precipitation process. In addition, the result error is larger in convective precipitation. The unevenness of the environmental wind field in convective precipitation can reduce the accuracy of wind measurement, and therefore it is not enough to explain the rationality of the AWR retrieved wind field. The wind profile radar is quite different from the AWR retrieved and the synthetic wind field. For different precipitation types, the wind field structure retrieved by AWR and the wind field obtained by AWR synthetic wind field are consistent with the basic characteristics of various weather systems. The spatial distribution and size direction of the horizontal wind field of two algorithms are very close. Error results show that the relative deviation of horizontal wind speed in the stable and convective precipitation is less than 19% and 29%, and the difference of horizontal wind direction is lower than 14.92° and 26.35°, respectively. The error is within the acceptable range. Compared with the AWR synthetic wind field, the retrieved wind field result during stable precipitation process is better than that during convective precipitation process.

[Objectives]Ship performance evaluation and optimization technology with regard to wind wave factors of real sea area is the key to ship energy efficiency design,and wind load is an important part of the environmental load of wind waves of ships in real sea area. For large container ships,the stacking mode has significant influence on the wind load characteristics of ships. In order to quantitatively study the influence rule,[Methods]series of wind tunnel tests with multiple wind speeds under full wind angle range are carried out for 10 000 TEU large container ship model with the same container loading quantity but different stacking modes. CFD numerical simulation calculation and Fujiwara's empirical formulas are applied for cross validation.[Results]Analysis results show that the shape parameters for transverse and longitudinal projected areas of ships exposed to the wind are of significant impact on wind load characteristics. Conventional stacking modes with streamlined or stepped layouts have better wind load characteristics in general. Special stacking modes with multiple vacancies on the deck occupy certain advantages in such aspects as yaw moment performance.[Conclusions] The study provides technical reference for container stacking mode design and calculation and evaluation of wind load in practical engineering.

(1) Background--The key state-owned forest areas are unique regions formed under the specific historical periods and special development strategies, and the reform of their governance system is crucial to ecological protection, sustainable development and social well-being in the new era. Although the reform of the state-owned forest areas has lasted for many years, there are still some problems such as dysfunctional institutional reform, stagnant industrial development and imperfect livelihood guarantee. (2) Methods--Taking Yichun Forest Industry Group in Heilongjiang Province as an example, this paper integrated resources, systems and social capitals into the analysis framework, and used the method of multiple case studies to compare and analyze the two reform modes of complete integration of the government and enterprises with the incomplete integration of the government and enterprises. The reform mechanisms and realistic dilemmas in the process of separating the government from enterprises were explored by setting SES (Social-ecological System) key variables in eight typical cases extracted from the four forestry bureaus. (3) Results--First, although the two reform modes of separating the government from enterprises had their own characteristics, in general, the reform of separating the government from enterprises led to the reshaping of the institutional environment and the changes in the authorities and responsibilities of the local governments and forest industry enterprises. However, the deep-rooted "system of units" and the deepening of path dependence limited the smooth implementation of the reform. Second, the reform process of separating the government from enterprises was externally influenced by the completeness of the supporting policies for reform, and internally constrained by the resources allocation rights, relative discourse rights and interdependence of the actors and governance systems, which affected the action stages by affecting the allocation mechanism of the reform costs. Third, the separation of the government from enterprises involved multiple stakeholders, including the government, enterprises, employees, etc., which would inevitably lead to the reconstruction of interest relations. In the post-reform period, it also faced the dilemmas such as unclear boundaries of the government and enterprises functions, insufficient support of the reform-related supporting policies, and difficult balance in the resettlement of the forest employees, which restricted the release of reform dividends. (4) Conclusions and Discussions--First, the local governments need to adjust their roles and responsibilities from those of administrators in the past to those of market regulators and service providers. At the same time, we should establish the value concept of overall co-governance and shape a mature cooperative governance structure. Second, since the reform of separating the government from enterprises affects the action stages of the local governments and forest industry enterprises, it is necessary to reasonably determine the sharing mechanism of reform costs and coordinate funding channels. For the public services undertaken by the enterprises in the transitional period, the government can share the relevant costs by purchasing socialized services. Third, after the separation of the government from enterprises, the forest workers have changed from the original "unit people" to enterprise employees. In the face of the rapidly changing development situation, the forest employees should continue to carry forward the forest industry spirit of hard work and daring to venture, actively face the market, go to the market and integrate into the market, constantly learn new vocational skills, and create "gold mountains and silver mountains" through diversified channels while managing and protecting the "clear waters and green mountains". [ABSTRACT FROM AUTHOR]

Objective To improve the risk management ability of medical staffs by conducting a hazard vulnerability analysis of the nephrology department in the fight against COVID-19. Methods A risk assessment group was set up to calculate the risk index of the COVID-19 outbreak in the nephrology department by using the Kaiser Permanente model. Related management strategies were carried out such as enhancing the screening and identification management, system for hospital visitors and caregivers, training and health education about COVID-19. To evaluate the knowledge of medical staffs in the nephrology department observe the condition of ask-wearing, body temperature and other issues of patients. Results After implementation of management strategies, there was an improvement in awareness rate of knowledge and implementation rate of hand hygiene among medical staffs. No infection in medical staffs, patients or their caregivers was found. Conclusion The hazard vulnerability analysis based on Kaiser Permanente model is an effective tool for the development of risk management ability of medical staffs in nephrology department. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of baicalein (BAI) on autophagy in gastric cancer cell line MGC-803. Methods MGC-803 cells were treated with 0, 5, 15, 25, 50 μmol/L BAI for 24, 48, 72 hours. The proliferation activity of MGC-803 cells was detected by MTT assay. Acridine orange (AO) staining combined with immunofluorescence cytochemical staining was performed to observe the expression of microtubule-associated protein 1 light chain 3 (LC3) and P62 to determine autophagy in MGC-803 cells. The protein levels of LC3, P62, phosphatidylinositol 3-kinase (PI3K), phosphorylated PI3K (p-PI3K), AKT, and p-AKT were detected by Western blot analysis. Results Compared with the control group, BAI significantly inhibited the proliferation of MGC-803 cells in a time- and dose-dependent manner. BAI-treated MGC-803 cells showed a significant increase in acid lysosomes and increased LC3 expression. BAI treatment significantly decreased phosphorylation of PI3K and AKT proteins, increased the ratio of LC3-II/LC3-I and up-regulated the expression of P62 protein. Conclusion Baicalein could inhibit PI3K/AKT signaling pathway and induce autophagy in MGC-803 cells.

Objective: To investigate the survival status and prognosis of patients with essential thrombocythemia(ET) and analyze the prognostic factors for the patients' survival, so as to provide a evidence for clinical treatment and prognosis evaluation., Methods: A retrospective analysis of 118 patients with ET was conducted in the Fifth Affiliated Hospital of Sun Yat-Sen University and Zhongshan Municipale People's Hospital from December 2002 to December 2013. The clinical characteristics were summarized, such as the survival curve and multi-factor analysis, therefore looking for the disease characteristics and risk factors affecting the survival and prognosis., Results: Among 118 ET patients enrolled in this study, the survival rate of ET patients for 1, 3, 5 and 10 years were 95.5%,92.6%,89% and 81.6%, respectively. Kaplan-Meier survival curve showed that the age ≥60 years old at diagnosis, cardiovascular risk factors, anamnesis of thrombosis or hemorrhage, anemia(hemoglobin<120 g/L), thrombocythemia (≥1 000×10 9 /L), risk stratification and hydroxyurea or HHT(hemoharringtonine) use in high-risk group were factors affecting the suvival rate, 7 out of those factors influencing survival rate were statistically significant (P<0.05). COX regression analysis showed that independent risk factors affecting survival have not yet been found., Conclusion: ET patients display a high survival rate and long survival time, and their conversion risk into the marrow fibrosis or leukemia has been found to be low. The age≥60 years old at diagnosis, cardiovascular risk factors, anamnesis of thrombosis or hemorrhage, anemia and therombocythemia are the risk factors affecting prognosis. The use of hydroxyurea or HHT in high-risk group can improve the prognosis.

Purpose: To discuss the clinical and pathological characteristics and management of parotid gland cyst., Methods: Forty-five resected samples of parotid gland tumors were selected from Shengjing Hospital of China Medical University from January 2000 to June 2014 whose postoperative pathological diagnosis were parotid gland cysts. The clinico-pathological characteristics in different types of parotid gland cysts were analyzed., Results: Of 45 cases of parotid gland cyst, 25 were males, 20 were females. The median age was 51 years old. 21 cases were located on the left side of parotid gland, while 24 on the right side. The main clinical symptom was painless swelling in parotid gland region. The sizes varied from 0.8 cm×0.6 cm×0.5 cm to 10 cm×2.8 cm×0.6 cm. Nine cases were accompanied with pain, and 36 without. 4 cases were seen with lymph node enlargement, and 41 without pain. The main treatment modality was simple resection of cyst without any injury of facial nerve. No recurrence was seen after following up for 1-10 years. Of the 45 postoperative histopathological results, 29 cases (64.4%) were simple cysts, 9 cases (20.0%) were polycystic disease cysts, and 7 cases (15.6%) were lymphoepithelial cysts. Besides the above-stated pathological manifestations, some non-specific microscopic changes were accompanied with parotid gland cysts, including tumor-like hyperplasia of fibrous tissue, inflammations, calcifications, synovial cyst-like changes, foreign-body giant cell reactions and granulomatous changes., Conclusions: The main symptom of parotid gland cyst is progressive enlargement, and simple resection of cyst is the first-line treatment without facial nerve injury or reoccurrence. The main pathological classifications are simple cysts,lymphoepithelial cysts and polycystic disease cysts.

Objective: To investigate the effect of pulsed electromagnetic tields (FEMFs) on osteogemc differentiation and Wnt/β-catenin signaling pathway in rat bone marrow mesenchymal stem cells (BMSCs)., Methods: Rat BMSCs were isolated and the passage 3 cells were divided into 3 groups. Cells were cultured in LG-DMEM complete medium for 1 d to ensure fully adherent. Then, change the medium. Cells were maintained in complete medium (Control group) or in osteo-induction medium (OM group). The cells in PEMFs group were cultured in complete medium and exposed to 8 Hz, 3. 8 mT PEMF stimulation for 40 min/d. The intervention lasted for 21 d. Cell proliferation activity was determined by using MTT. The effects of PEMF onosteogenic differentiation were assessed by ALP and Alizarin Red S staining. Various osteoblast-relevant genes and genes of Wnt/β-catenin signaling were analyzed by quantitative real-time RT-PCR., Results: We. found that OM could significantly promote the proliferation of BMSC at 7 d, 14 d, 21 d (P<0. 05), but the effect was not obviously found in PEMFs group. For osteogenic differentiation, the positive rates of ALP or Alizarin Red S staining were detected higher in PEMFs/ OM group (P < 0. 05). Quantitative RT-PCR revealed PEMFs or OM could increase mRNA levels of Wnt1, Wnt3a, LRP5, β-catenin, BMP-2, Runx2, ALP, OC at special time point (P<0. 05). Compared to OM group, PEMFs have a lower expression in each detection, but the trends were consistent., Conclusion: PEMFs (8 Hz, 3. 8 mT) could induce the osteogenic differentiation of rat BMSCs via activating Wnt/β-catenin signaling pathway.

Objective: To investigate the effects of whole body vibration therapy (WBVT) on structural and functional remodeling of subchondral bone in rabbits with early Osteoarthritis (OA)., Methods: Twenty-four adult rabbits received anterior cruciate ligament transaction (ACLT) to establish knee osteoarthritis model. The rabbits were randomly divided into two groups: ACLT group and WBVT+ ACLT group. The rabbits in the WBVT+ ACLT group received whole body vibration treatment (Frequency: 40 Hz; Amplitude: 2-4 mm; 40 min/d, 5 d/week, 4 weeks) two months after surgery. After interventions, the left femur and tibia of all rabbits were placed in a Micro-CT scanning system. The bone volume fraction (BVF), trabecular thickness (Tb. Th), trabecular spacing (Tb. Sp), trabecular number (Tb. N), volumetric BMD (vBMD), and tissue BMD (tBMD) of the bones of femoral condyle and tibia were analysed using three-dimensional reconstruction Micview V2.1.2 and ABA analysis software. Geomagic Studio 11.0 software were used to calculate modulus (EM), reaction force (RF) and average Von Miss stress (VMF)., Results: Higher levels of BVF, Tb. N, Tb. Th, EM, RF, VMF, vBMD and tBMD and lower levels of Tb. Sp were found in distal femora and tibia of the rabbits in the WBVT+ACLT group compared with the controls (P < 0.05)., Conclusion: WBVT can effectively improve bone microstructure and mechanical properties of rabbits with early knee osteoarthritis.

Objective: To study the optimum extraction technology of total flavonoids and total triterpenoids from Tetrastigma planicaule and their antioxidant activity., Methods: Optimized the extraction of total flavonoids and total triterpenoids using an L9 (3(4)) orthogonal array design, and the antioxidant activity was extimated by FRAP assay, salicylicl acid assay and ABTS assay., Results: The best extraction conditions for total flavonoids from Tetrastigma planicaule were as follows: 70 degrees C of 70% ethanol ultrasound-assisted extracting for 1 h and extracting three times, and total triterpenoids was:70 degrees C of 60% ethanol microwave extracting for 5 min and extracting two times. Compared with the positive control samples VC and tea polyphenol, the activity of reducing Fe3+ antioxidant and scavenging ABTS + * of flavonoids from Tetrastigma planicaule was higher than that of tea polyphenol, but scavenging OH * was lower than theirs;, Conclusion: The method of using ultrasound-assisted extraction to extract total flavonoids and microwave extraction to extract total triterpenoids is the best, the extracts of Tetrastigma planicaule also shows certain antioxidative activity.

Aim: To evaluate cytokine production by CD4(+); T cells and frequency of multifunctional CD4(+); T cells after stimulation with ESAT-6 peptide pool. To understand the role of ESAT-6 specific CD4(+); T cells in the control of local TB infection., Methods: PFCs were isolated from patients with tuberculous pleurisy, and assessed by flow cytometry for cytokine production, subpopulation, frequency and function of multifunctional CD4(+);T cells after stimulation with ESAT-6 peptide pool., Results: Following stimulation with BCG, ESAT-6 peptide pool and recombinant ESAT-6 protein, CD4(+); but not CD8(+); T cells expressed IFN-γ, IL-2 and TNF-α. Distinct from BCG, ESAT-6 peptide pool and recombinant ESAT-6 protein induced similar frequencies of IFN-γ, IL-2 and TNF-α. High frequency of multifunctional CD4(+);T cells was observed. Analysis of mean fluorescence intensity (MFI) indicated that triple positive cells produced most amounts of cytokines on single cell level compared with double positive and single positive cells., Conclusion: ESAT-6 peptide pool predominantly induced Th1 cytokine production by CD4(+);T cells in PFCs. Multifunctional CD4(+); T cells were observed and secreted most amounts of cytokines on single cell level, suggesting that these cells probably played essential role in local TB infection.

Objective: To evaluate cytokine production and expression of γδ T cells within pleural fluid cells (PFCs) from patients with tuberculous pleurisy following bacille calmette guerin (BCG) stimulation., Methods: PFCs were isolated from patients with tuberculous pleurisy, and assessed for cytokine production, cell subpopulation, phenotype and characterization of T cell receptors after stimulation with BCG. The positive PCR products were further labeled with fluorescence and analyzed by genescan technique to determine the CDR3 size and evaluate the clonality of the detectable TCR Vγ and Vδ T cells., Results: Following stimulation with BCG, the positivity of interferon-γ (IFN-γ)-producing CD(4) T cells and γδ T cells were 0.38% and 5.35%, respectively. Phenotypic analysis indicated that the majority of IFN-γ(+)γδ(+) T cells expressed CD(45RO)(+) (73.5%). In addition, δ(2) T cells produced IFN-γ (11.1%) and TNF-α (25.5%). After expansion with BCG for 3 weeks, cells were harvested and mRNA extracted and RT-PCR conducted to amplify cDNA with 3 primers for Vγ and 8 primers for Vδ. The results indicated that BCG selectively expanded δ(2) T cells with oligoclonal peak in Vδ(2) cells., Conclusions: BCG induced memory γδ and δ(2) T cells to produce cytokines in PFCs. Genescan analysis showed that Vδ(2) displayed oligoclonality.

In order to explore the feasibility of inducing the human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) to differentiate into insulin-secreting cells with biological products alone, hUC-MSCs were separated and purified from the whole umbilical cord by the sequent digestion of collagenase II and trypsin followed by two-step centrifugation. hUC-MSCs were induced with IMDM culture medium containing epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and Ginkgo biloba extract (GBE). Before and after the induction, the morphological changes were observed under inverse microscope; the islet-related genes were detected by RT-PCR; islet-like clusters (ILCs) were identified by dithizone (DTZ) staining; PDX-1 and immunoreactive insulin (IRI) were examined by immunofluorescence method; the quantity and quality of IRI secretion were assayed by chemiluminescence immunoassay and Western blot respectively. The results showed that the purified hUC-MSCs presented long spindle-like shape and parallel or spiral arrangement which are typical morphological features of MSCs. After the induction, hUC-MSCs changed gradually into round or oval shape and gathered together to form ILCs; there were more than one hundred clusters on the growth surface of a flask of T25; ILCs were stained into positive mauve by DTZ and positive for PDX-1 and IRI; Western blot displayed that most of the IRI was proinsulin (PI). Therefore, hUC-MSCs can rapidly differentiate into insulin-secreting cells under the sole induction of EGF, bFGF, GBE and IMDM, but ILCs are not mature enough to produce sufficient true insulin.

Objective: To investigate the role of hepatic stellate cells in the differentiation of hepatic oval cells into adult hepatocyte., Methods: The oval cell were cocultured with primary hepatic stellate cells (HSC) in the same well (M-coculture) or separately cultured with HSC by millIcell (S-coculture). Oval cells were cultured alone as control; the expression of adult hepatocyte marker HNF-4alpha, albumin, and oval cell marker AFP, CK-19 in each group were detected by real-time PCR and western-blot. Phenotype changes were observed by transmission electron microscope (TEM); PAS staining was used to detect the quantity of glycogen granule in oval cell. Albumin level in supernatant was detected using ELISA kit., Result: (1) The relative level of HNF-4alpha and albumin mRNA expression compared with pre-coculture: M-coculture: HNF-4a: 1.9+/-0.2, 10.7+/-1.2, 12.0+/-1.3; albumin: 5.7+/-1.6, 110.7+/-13.7, 173.6+/-22.3. S-coculture: 1.4+/-0.1, 3.2+/-0.6, 8.9+/-1.4 times; albumin: 2.9+/-1.4, 22.3+/-8.5, 96.3+/-16.3. The relative level of HNF-4a and albumin mRNA expression in coculture group (M- and S-coculture) were higher than control group (LSD-t: 32.98, 10.08, 13.38, 7.96; P less than 0.01); and a higher level of HNF-4a and albumin was found in M-coculture group compared to S-coculture group (LSD-t: 32.98, 25.65; P less than 0.01). The relative level of AFP and CK-19 mRNA expression compared with pre-coculture: M-coculture: 1.1+/-0.2, 0.2+/-0.0, 0.0+/-0.0; S-coculture group: AFP: 1.0+/-0.2, 0.2+/-0.1, 0.1+/-0.0; CK-19: 0.6+/-0.1, 0.1+/-0.0, 0.0+/-0.0; control group: AFP: 1.0+/-0.1, 1.0+/-0.1, 1.1+/-0.1, CK-19: 1.0+/-0.1, 1.1+/-0.1, 1.0+/-0.1. The relative level of AFP and CK-19 mRNA expression in coculture group (M- and S-coculture) were lower than that in control group (LSD-t: 37.99, 34.50, 13.59, 22.46; P less than 0.01). (2) The albumin secretion was detected in M-coculture: 14 day: (15.30+/-0.09) ng/ml, 21: (20.98+/-0.12) ng/ml; S-coculture: 14 day: (11.41+/-0.13) ng/ml, 21 day:(15.12+/-0.17) ng/ml. (3) It showed more organelles such as endoplasmic reticulum, mitochondrion and Golgi apparatus in oval cells cocultured with HSC. And cholangiole-like structure appeared between oval cells cocultured with HSC. (4) PAS staining showed glycogen granules could be observed in coculture groups., Conclusion: HSC can induce differentiation of oval cell into mature hepatocyte.