1. Role of epithelial-mesenchymal transition in the enrichment of colorectal cancer stem cells
- Author
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Jia-ping CHENG, Jiang LI, Xuan SU, Yi-lin CHEN, Qing-liang ZENG, and Kun-ming WEN
- Subjects
lcsh:R5-920 ,neoplastic stem cells ,lcsh:R ,epithelial-mesenchymal transition ,lcsh:Medicine ,colorectal neoplasms ,lcsh:Medicine (General) - Abstract
Objective To explore whether the enrichment of cancer stem cells (CSCs) in colorectal cancer by suspension culture method is involved with epithelial-mesenchymal transition (EMT). Methods 3D microspheres were cultured by suspension culture method to human colorectal cancer SW620 cells. The 3D microspheres and SW620 cells were used as the research objects. To clarify whether 3D microspheres were enriched with CSCs, we made tumorigenicity experiments in NOD/SCID mice, soft agar cloning experiments, and detected the expression levels of cancer stem cells markers CD44 and Ep-CAM by flow cytometry or by Western blotting. The protein expression levels of EMT markers such as E-cadherin, N-cadherin and vimentin were detected by Western blotting. Results Compared with the parental SW620 cells, colony formation in vitro (P
- Published
- 2016