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Magnetoencephalography is a non-invasive technology for brain function imaging, which is of enormous value to brain science research and clinical application due to its ultra-high temporal and spatial trace resolution. In this paper, we introduce a self-built and atomic magnetometer based wearable magnetoencephalography system. By designing bi-planar coils system and combining with reference sensor array, the residual magnetic field in the subject's head movement area is controlled to be within ±1 nT, which ensures the sensors are maintained within their dynamic range during the moving measurement. At the same time, a virtual gradiometer-based noise reduction method is proposed to suppress the common-mode magnetic-field noise. Finally, the alpha rhythm and auditory evoked magnetic field signals with high signal-to-noise ratio are successfully detected under the subject's natural head movement and the effectiveness of the system is confirmed. This study could provide more possibilities for the application and promotion of moving wearable magnetoencephalography.

In order to obtain optimized production technology of Castanopsis hystrix veneer, promote the high value utilization of Castanopsis hystrix plantation wood. In this study, Eucalyptus plywood was taken as the research object, the influences of glue and hot pressing process (glue spread, hot pressing temperature, hot pressing time and hot pressing pressure) on the moisture content, dipping peeling property and surface bonding strength of the Castanopsis hystrix veneer were investigated through orthogonal test method. Besides, the effects of different kinds of varnishes (nitrocellulose varnish, waterborne varnish and polyurethane varnish) on the color difference parameters and the physicochemical properties of the paint film (resistance to impact, wearability, adhesion, pencil hardness) of the veneer were also investigated. The results showed that the moisture content, dipping peeling property and surface bonding strength of the veneer could meet the requirements of the national standard. The optimal hot pressing process was 120 g/m² of glue, 180 °C of hot pressing temperature, 60 s of hot pressing time, and 0.4 MPa of hot pressing pressure. The impact resistance, abrasion resistance, and adhesion of the paint film coated with nitrocellulose varnish and polyurethane varnish could meet the requirements of the national standard. However, the abrasion resistance of the paint film coated with water-based varnish could not meet the requirements of the national standard, but it had the best adhesion and impact resistance. The Castanopsis hystrix wood has great development potential. [ABSTRACT FROM AUTHOR]

The widespread and frequent use of antibiotics to treat diseases or encourage animal growth has resulted in their persistence and accumulation in water, soil, and sediments. As a typical emerging pollutant in the environment, antibiotics have become an important research focus in recent years. Antibiotics are commonly found at trace levels in water environments. Unfortunately, the determination of various types of antibiotics, all of which exhibit different physicochemical properties, remains a challenging endeavor. Thus, developing pretreatment and analytical techniques to achieve the rapid, sensitive, and accurate analysis of these emerging contaminants in various water samples is an essential undertaking.In this paper, a solid phase extraction-high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS) method for the simultaneous determination of 22 antibiotics including 4 penicillins, 12 quinolones and 6 macrolides in environmental water samples was developed. Based on the characteristics of the screened antibiotics and sample matrix, the pretreatment method was optimized, focusing on the SPE column, pH of the water sample, and amount of ethylene diamine tetra-acetic acid disodium (Na 2 EDTA) added to the water sample. Prior to extraction, a 200 mL water sample was added with 0.5 g of Na 2 EDTA and pH-adjusted to 3 using sulfuric acid or sodium hydroxide solution. Water sample enrichment and purification were achieved using an HLB column. HPLC separation was carried out on a C 18 column (100 mm×2.1 mm, 3.5 μm) via gradient elution with a mobile phase composed of acetonitrile and 0.15% (v/v) formic acid aqueous solution. Qualitative and quantitative analyses were performed on a triple quadrupole mass spectrometer in multiple reaction monitoring mode using an electrospray ionization source. The results showed correlation coefficients greater than 0.995, indicating good linear relationships. The method detection limits (MDLs) and limits of quantification (LOQs) were in the ranges of 2.3-10.7 ng/L and 9.2-42.8 ng/L, respectively. The recoveries of target compounds in surface water at three spiked levels ranged from 61.2% to 157%, with relative standard deviations (RSDs) of 1.0%-21.9%. The recoveries of target compounds in wastewater at three spiked levels were 50.1%-129%, with RSDs of 1.2%-16.9%. The method was successfully applied to the simultaneous determination of antibiotics in reservoir water, surface water, sewage treatment plant outfall, and livestock wastewater. Most of the antibiotics were detected in watershed and livestock wastewater. Lincomycin was detected in 10 surface water samples, with a detection frequency of 90%, and ofloxacin showed the highest contents (127 ng/L) in livestock wastewater. Therefore, the present method exhibits excellent performance in terms of MDLs and recoveries compared with previously reported methods. The developed method presents the advantages of small water sample volumes, wide applicability, and fast analysis times; thus, it can be considered a rapid, efficient, and sensitive analytical method with excellent potential for monitoring emergency environmental pollution. The method could also provide a reliable reference for formulating antibiotic residue standards. The results provide strong support for and an improved understanding of the environmental occurrence, treatment, and control of emerging pollutants.

Objective: To compare the effect of electroacupuncture (EA), metformin and EA plus metformin on the cognitive ability and senile plaques (SPs) in cerebral cortex and hippocampus of Alzheimer's disease (AD) mice, so as to explore a better treatment method for AD., Methods: Twenty-four male APP/PS1 mice were randomly divided into model, metformin (medication), EA and EA+medication groups, with 6 mice in each group. Other 6 male wild C57 mice were used as the control group. EA (2 Hz, 1.0 mA) was applied to "Baihui" (GV20) and "Shenshu" (BL23) for 15 min, once a day, for 4 weeks, with 1 day's off every week. The mice of the medication group received gavage of metformin (300 mg·kg -1 ·d -1 ) once a day for 4 weeks. Morris water maze tests were used to assess the cognitive function of mice. H.E. staining was used to observe the histopathological changes of neurons in the cortex and hippocampus. Immunohistochemical method was used to observe the cerebral cortex and hippocampal SPs. The expression levels of SPs formation-related proteins: β-site amyloid precursor protein cleaving enzyme 1(βACE1) and insulin-degrading enzyme (IDE) in the cortex and hippocampus were detected by Western blot., Results: Compared with the control group, the escape latency, number of SPs and the expression of βACE1 in the cortex and hippocampus were ob-viously increased ( P <0.01), and the times of platform quadrant crossing and the expression of IDE protein were markedly decreased in the model group ( P <0.01). In comparison with the model group, the escape latency, and the number of SPs and expression of βACE1 proteins in the cortex and hippocampus in the 3 treatment groups were significantly down-regulated ( P <0.01), while the times of platform quadrant crossing, and the expression of IDE protein in both cortex and hippocampus of the three treatment groups were considerably up-regulated ( P <0.01). Comparison among the three treatment groups showed that the therapeutic effect of EA+medication was significantly superior to that of medication and simple EA in down-regulating the escape latency, the number of SPs and expression of βACE1 in the cortex and hippocampus ( P <0.01), and in up-regulating the times of the platform quadrant crossing, and expression of IDE protein in both cortex and hippocampus ( P <0.01). No significant differences were found between the simple medication and simple EA in all the indexes mentioned above ( P >0.05)., Conclusion: EA, metformin and EA plus metformin can improve cognitive ability and relieve SP formation in cerebral cortex and hippocampus in AD mice, which may be associated with their functions in down-regulating the expression of βACE1 and up-regulating the expression of IDE. The therapeutic effects of EA plus metformin are apparently better than those of simple EA and simple metformin.

Objective: To observe the effect of electroacupuncture (EA) on the expression of insulin phosphatidylinositol-3 kinase/glycogen synthetase kinase-3α (PI3K/GSK3α) signal pathway related proteins in the hippocampus in mice with Alzheimer's disease (AD), and to explore the regulatory mechanism of EA on improving the pathological characteristics of AD., Methods: Twelve male APP/PS1 double transgenic mice were randomly divided a model group and a treatment group, 6 mice in each group; another 6 wild-type male mice were taken as the control group. The mice in the treatment group were treated with EA (continuous wave, 2 Hz of frequency) at "Baihui" (GV 20) and bilateral "Shenshu" (BL 23), once a day; 7-day treatment was taken as a course of treatment, and 2 courses of treatment were given. The immunohistochemistry method and Western blot method were used to detect the distribution and expression level of hippocampal PI3K/GSK3α signal pathway related proteins P85α, P110α, GSK3α and pS 21 GSK3α, and the number of hippocampal senile plaques (SP) was observed., Results: The proteins of P85α, P110α, GSK3α and pS 21 GSK3α were mainly distributed in the cytoplasm of hippocampal neurons, and the GSK3α was also distributed in the axons of neurons in the model group and the treatment group. The immunohistochemistry results showed that the distribution level of GSK3α in the hippocampus in the model group was significantly higher than that in the control group ( P <0.001), and the distribution level of pS 21 GSK3α, P85α and P110α was significantly decreased ( P <0.01, P <0.001); compared with the model group, the distribution level of GSK3α in the hippocampus in the treatment group was significantly decreased ( P <0.001), and the distribution level of pS 21 GSK3α, P85α and P110α in hippocampus was significantly increased ( P <0.05, P <0.001). The Western blot results showed compared with the control group, the expression of pS 21 GSK3α, P85α and P110α as well as the ratio of pS 21 GSK3α/GSK3α in the hippocampus in the model group were significantly decreased ( P <0.001), and the expression of GSK3α was increased ( P <0.05); compared with the model group, the expression of pS 21 GSK3α, P85α, P110α and the ratio of pS 21 GSK3α/GSK3α in the hippocampus in the treatment group were significantly increased ( P <0.01, P <0.001), and the expression of GSK3α was decreased ( P <0.05). Compared with the control group, the number of hippocampal SP in the model group was significantly increased ( P <0.001); compared with the model group, the number of hippocampal SP in the treatment group was significantly decreased ( P <0.01)., Conclusion: EA could effectively regulate the expression of PI3K/GSK3α signal pathway related proteins in the hippocampus in mice with AD, so as to reduce the formation and deposition of SP.

Zhenjiu Dacheng ( Compendium of Acupuncture and Moxibustion ) contains a large number of acupuncture medical cases, involving a variety of illnesses, and more cases of the treatment of cancer. The medical cases in Compendium of Acupuncture and Moxibustion point out that the tumor is a tangible evil, and its pathological factors are often regarded as sputum. It should be diagnosed and treated early, and it is easy to be dangerous if delayed. Acupuncture treatment should be based on the treatment of tumor itself, in the consideration of consolidating the root and cultivating the primary, treatment should be clear about the condition of the disease, pay attention to the timing of acupuncture, selecting correctly the reinforcing or reducing technique, emphasizing the use of moxibustion. The academic characteristics of Compendium of Acupuncture and Moxibustion medical treatment of tumors have had a profound impact on later generations, and it is still of great significance for guiding clinical acupuncture treatment of tumor diseases today.

Myeloblastosis (MYB) transcription factors are one of the largest families of transcription factors in higher plants. They play an important role in plant development, defense response processes, and non-biological stresses, i.e., drought stress. Foxtail millet (Setaria italica L.), originated in China, is resistant to drought and low nutrition stresses and has been regarded as an ideal material for studying abiotic stress resistance in monocotyledon. In this study, we ran a transcription profile analysis of zheng 204 under low-nitrogen conditions and identified a MYB-like transcription factor SiMYB42, which was up-regulated under low-nitrogen stress. Phylogenetic tree analysis showed that SiMYB42 belongs to R2R3-MYB subfamily and has two MYB conserved domains. Expression pattern analysis showed that SiMYB42 was significantly up-regulated under various stress conditions, including low-nitrogen stress, high salt, drought and ABA conditions. The results of subcellular localization, quantitative real-time PCR and transcriptional activation analysis indicated that SiMYB42 protein localizes to the nucleus and cell membrane of plant cells, mainly expressed in the leaf or root of foxtail millet, and has transcription activation activity. Functional analysis showed that there was no significant difference between transgenic SiMYB42 Arabidopsis and wild-type (WT) Arabidopsis under normal conditions; however, under low-nitrogen condition, the root length, surface area and seedling fresh weight in transgenic SiMYB42 Arabidopsis, were significantly higher than their counterparts in WT. These results suggest that SiMYB42 transgenic plants exhibit higher tolerance to low-nitrogen stress. Expression levels of nitrate transporters genes NRT2.1, NRT2.4 and NRT2.5, which are the transcriptional targets of SiMYB42, were higher in transgenic SiMYB42 Arabidopsis plants than those in WT; the promoter regions of NRT2.1, NRT2.4 and NRT2.5 all have MYB binding sites. These results indicate that SiMYB42 might enhance foxtail millet tolerance to low-nitrogen condition through regulating the expression of nitrate transporter genes. This study reveals the possible functions of SiMYB42 in a low-nitrogen stress response pathway, and provides a foundation for further understanding the entire regulation network of foxtail millet in response to low-nitrogen stress.

Objective: To compare the toxic effects of three different particles on vascular endothelial cells, and to investigate the influences of particle composition and sizes on the cardiovascular toxic effects., Methods: Nano-SiO2 particles, nano-TiO2 particles, and standard quartz particles were selected as the test substances, and the nano-TiO2 particles and standard quartz particles were used as composition controls and size controls, respectively. The human umbilical vein endothelial cells were exposed to different doses (5.0, 10.0, 20.0, 40.0 µg/ml) of the three particles as well as particle-free DMEM medium (0 µg/ml dust) for 24 h. Then, the culture supernatants were collected, and the activities of lactic dehydrogenase (LDH) and total superoxide dismutase (SOD) as well as the releases of NO, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) were measured., Results: Compared with those of 0 µg/ml dose group, the LDH activities of all nano-SiO2 groups, 10.0, 20.0, and 40.0 µg/ml nano-TiO2 groups, and 10.0, 20.0, and 40.0 µg/ml standard quartz groups were significantly increased (P < 0.01); the SOD activities of 5.0, 10.0, and 20.0 µg/ml nano-SiO2 groups, 40.0 µg/ml nano-TiO2 group, and 20.0 and 40.0 µg/ml standard quartz groups were significantly increased (P < 0.05), but that of the 40.0 µg/ml nano-SiO2 group was significantly decreased (P < 0.01); the TNF-α releases of 10.0, 20.0, and 40.0 µg/ml nano-SiO2 groups, all nano-TiO2 groups, and 40.0 µg/ml standard quartz group were significantly increased (P < 0.01); the IL-6 releases of 10.0, 20.0, and 40.0 µg/ml nano-SiO2 groups, 20.0 and 40.0 µg/ml nano-TiO2 groups, and 40 µg/ml standard quartz group were significantly increased (P < 0.01). When the dust doses were 5.0, 10.0, 20.0, and 40.0 µg/ml, the LDH activities of nano-SiO2 groups were significantly higher than those of standard quartz groups (P < 0.05); when the dust doses were 10.0, 20.0, and 40.0 µg/ml, the LDH activities of nano-TiO2 groups were significantly lower than those of standard quartz groups (P < 0.05). When the dust doses were 5.0, 10.0, 20.0, and 40.0 µg/ml, the SOD activities of nano-SiO2 groups were significantly higher than those of standard quartz groups (P < 0.01); when the dust dose was 20 µg/ml, the SOD activity of nano-TiO2 group was significantly higher than that of standard quartz group (P < 0.01). When the dust doses were 5.0, 10.0, 20.0, and 40.0 µg/ml, the TNF-α releases of nano-SiO2 groups were significantly higher than those of standard quartz groups (P < 0.01); when the dust doses were 5.0 and 10.0 µg/ml, the TNF-α releases of nano-TiO2 groups were significantly higher than those of standard quartz groups (P < 0.01). When the dust doses were 5.0, 10.0, 20.0, and 40.0 µg/ml, the IL-6 releases of nano-SiO2 groups were significantly higher than those of standard quartz groups (P < 0.05); when the dust doses were 20 and 40 µg/ml, the IL-6 releases of nano-TiO2 groups were significantly higher than those of standard quartz groups (P < 0.05)., Conclusion: All the three particles are able to exert certain toxic effects on vascular endothelial cells. Nano-SiO2 particles have the most toxic effects, and nano-TiO2 particles and standard quartz particles show uncertain effects. The toxicity of particles is linked to their composition and sizes.

Objective: To identify the different plant resources of Herba Rabdosiae Serrae by using Random Amplified Polymorphic DNA ( RAPD ) Analysis., Methods: The mini spin columns were used to extract the genomic DNA from five different plants of Herba Rabdosiae Serrae. With the DNA extracted from these plants as template,the 85 oligo nucleic acids (10 bp)as random primers,the polymer chain reaction (PCR) was done and the results were analysed by electro-pharoses., Results: 12 primers were selected with polymorphism and 7 of them showed good polymorphism in RAPD map., Conclusion: RAPD method can be used to identify the plant resources of Herba Rabdosiae Serrae.