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Based on the interprovincial differences in China's environmental protection tax, it is of great practical significance for economic transformation and upgrading, as well as ecological civilization construction, to evaluate whether China's environmental protection fee-to-tax reform (CEPFR) effectively enhances corporate green technology innovation by means of tax burden classification. This study constructed a quasi-natural experiment based on typical facts of the formal implementation of the Environmental Protection Tax Law of the People's Republic of China in 2018. It utilized panel data from Chinese A-share listed companies between 2013 and 2022 and employed the difference-in-differences method to examine the impact of CEPFR on corporate green technology innovation and to evaluate the differential impact of varying degrees of tax burden increases on such innovation. The research results showed that: 1 CEPFR significantly promoted corporate green technology innovation, and this conclusion remained robust after a series of robustness tests. 2 The tax burden classification test found that the green innovation incentive of CEPFR primarily benefited from the tax burden increases at the middle and low levels rather than at the high level. 3 Heterogeneity analyses demonstrated that the foundation established by previous innovation and the pressure from media attention were more conducive to expanding the driving effects of tax burden increases at the middle and low levels on corporate green technology innovation. In addition, the green innovation incentives of tax burden increases primarily targeted substantive innovations such as invention patents, which could promote both the quantity and quality of corporate green technology innovation. Furthermore, tax burden increases could encourage enterprises to actively engage in green innovation cooperation. 4 A high-level environmental tax burden could improve the production efficiency of enterprises, but it could find it difficult to promote corporate green transformation. In contrast, the tax burden increases at the middle and low levels could optimize the green production efficiency of enterprises. From the perspective of tax burden classification, this study provides microevidence on the implementation effects of CEPFR and offers theoretical support and empirical references for local government to formulate appropriate tax regulatory intensity. [ABSTRACT FROM AUTHOR]

Objective To investigate the expression of TP53, PDGF and EGFR in primary astrocytomas, and analyze their correlation with clinicopathological features and prognosis. Methods We analyzed retrospectively the clinicopathological data of 90 patients with primary astrocytoma. The expressions of TP53, PDGF and EGFR in primary astrocytoma tissue samples were detected by immunohistochemistry. The survival of patients was followed up and Cox regression analysis was used to determine the prognostic factors. Results TP53 was expressed in the nucleus, PDGF and EGFR were expressed in the cytoplasm and cell membrane. The positive expression rates of TP53, PDGF and EGFR were 58.89%, 51.11% and 48.89%, significantly higher than those in normal brain tissues (all P < 0.05); the positive expression rate of TP53 in patients with tumor size ≥3 cm was higher than that in patients with tumor size < 3 cm. The positive expression rates of TP53, PDGF and EGFR in patients with WHO stages Ⅲ-Ⅳ were higher than those in patients with WHO stageⅠ-Ⅱ(all P < 0.05); the survival time of patients with positive expression of TP53, PDGF and EGFR were shorter than those of negative expression (all P < 0.05). Cox regression analysis found that WHO staging, TP53, PDGF and EGFR were all factors influencing the prognosis of primary astrocytomas patients. Conclusion TP53, PDGF and EGFR are highly expressed in primary astrocytomas and closely related to tumor progression. They are factors that affect the prognosis of patients.

Chemical furnace ash was generated during the use of pernicious metal alloy catalytic net, which has high recovery value. Because of the ash contains organic matter and oxidized stateRhodium, it difficult to dissolve. In this paper, hydrogen was reduced after ignition, and hydrochloric acid-hydrogen peroxide solution was used for airtight digestion, the obtained solution was determined by ICP-AES. For Chemical catalytic furnace ash with Rhodium concentration rage of 0.01%~5.0%, the relative standard deviation (RSD) was less than 2% (n=7), and the recovery was 95.50%~104.46%. The result were in agreement with the nominal values of quality control sample, meeting the requirement for recycling trade. [ABSTRACT FROM AUTHOR]

The video synthetic aperture radar (VideoSAR) technology is widely used in military reconnaissance, geological exploration, and disaster prediction, among other fields. Owing to multiple interference factors in SAR videos, such as speckle noise, specular reflection, and overlay effect, moving targets are easily mixed with background or other targets. Therefore, this study proposed an effective VideoSAR target detection and tracking algorithm. Firstly, several features of VideoSAR were extracted to construct multichannel feature maps. Then, deeper features were extracted using the improved lightweight EfficientDet network, thus improving the accuracy of SAR target detection while considering algorithm efficiency. Finally, the trajectory association strategy based on bounding boxes was employed to associate the same target in VideoSAR. The experimental results show that the method proposed in this study is effective for SAR shadow target detection and tracking. [ABSTRACT FROM AUTHOR]

A shape reconstruction method is investigated for real-time condition assessment control and health monitoring of spar wing structures. In the method strain sensors are used to obtain strain measurements from the target structure and to reconstruct the corresponding displacement based on the Ko displacement theory. The method is examined through an experimental uniform strength beam model. Then a finite element model of wing spar is built to verify the shape reconstruction method. Both the results show the feasibility and reliability of the method. [ABSTRACT FROM AUTHOR]

The article presents a study which discusses the lexicographic multi-objective disruption management model based on the methodology of disruption management in resolving problems disruptions in a single machine scheduling. Noted is that the model adopts customer dissatisfaction as a measurement of deviation. Computational results have proven that the model has lower system disturbance that the global rescheduling model.

Monodispersed polystyrene microspheres with 8.21 were prepared successfully by dispersion polymerization process. The dispersion coefficient of most particle sizes (PS) could be controlled within 0.04 and crosslinked degree was about 12.60/ó0. The major factors that affect the particle size and size distribution were investigated such as the dosage and mode of adding DVB crosslinked agent, concentrations of monomer, initiator and stabilizer, and ascorbic acid. It is concluded that, the prepared polystyrene microspheres have good mono-dispersion, low dispersion coefficient, large particle size and highly cross linked degree under the following appropriate synthesized process. The mass fraction of styrene monomer is 28.8~~33.5%. The mass ratio of initiator: azodiisobutyronitrile and St is 0.03~0.06. The mass ratio of stabilizer: polyvinyl-pyrrolidone and St is 0.5 ~0.8. DVB crosslinked agent is add in the solution drop by drop, the mass ratio of cross linked agent: divinylbenzene and St is 0.02~0.08. DVB should be released completely within 2 h after the reaction began to 6 h. [ABSTRACT FROM AUTHOR]

To further investigate the application of positron emission tomography (PET) technology in acupuncture research field, with "PET" and "acupuncture" as keywords, the related literature published from 1997 to 2018 was searched in PubMed, CNKI and WANFANG database; then the literature was classified and analyzed. The results showed that in clinical and experimental studies, whether in physiological or pathological conditions, PET technology has verified the specificity of acupoints, bidirectional regulation of acupoints, and the clinical effect of qi -arrival from the level of brain functional activity. It has deeply revealed the central mechanism underlying that acupuncture has multi-target, multi-channel and multi-level overall effects. The purpose of this study is to provide objective scientific basis for acupuncture research, and then potentially guide the clinical practice.

Objective: To observe the effect of twirling-reducing needling on changes of profile of differentially-expressed genes in the hypothalamus in rats with stress-induced prehypertension (SI-PHT), so as to explore its mechanism under-lying improvement of prehypertension., Methods: Wistar male rats were randomly divided into blank control group ( n =12), model group ( n =12) and acupuncture group ( n =10). The SI-PHT model was established by planta-electric-shock stimulation (30 V, 5 s) plus noise stimulation (80-100 db, 50 ms), twice daily, continuously for 11 days. Manual acupuncture stimulation (twirling-reducing needling) was applied to bilateral "Taichong" (LR 3) and "Quchi" (LI 11) for about 1 min (60 cycles/min), followed by retaining the needles for 20 min during modeling preparation, once daily for 11 days. The systolic blood pressure (SBP) and behavior changes were recorded before intervention and on the 3 rd , 5 th , 7 th , 9 th and 11 th day during modeling. The expression (Fold change,|FC| ≥ 1.5) profile of genes in the hypothalamus was analyzed using Affymetrix GeneChip Rat Gene 2.0 ST Array, followed by analyzing the differentially-expressed genes involving the related biological process,molecular function, and signaling pathways by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), separately., Results: After modeling, the systolic pressure levels were remarkably increased from the 3 rd day to the 11 th day in both model and acupuncture groups relatively to their respective baseline levels ( P <0.01), and were significantly lower in the acupuncture group than in the model group ( P <0.01) from the 5 th day to the 11 th day. Meanwhile, 93 genes were up-regulated and 153 down-regulated in the model group relative to the control group ( P <0.05,|FC| ≥ 1.5), while in comparison with the model group, 59 genes were up-regulated and 86 down-regulated in the acupuncture group ( P <0.05,|FC| ≥ 1.5). The expression levels of the 40 differen-tially-expressed genes (24 up-regulated and 16 down-regulated) in the model group were evidently reversed after acupuncture stimulation ( P <0.05,|FC| ≥ 1.5). After acupuncture stimulation, the expression of acyl-CoA synthetase medium-chain family member 3 (Acsm 3) gene was down-regulated, and chorionic gonadotropin-alpha (Cgα), crystal (Cry) αB and thyroid stimulating hormone beta (Tshβ) genes and the autoimmune thyroid disease (AITD) pathway were up-regulated ( P <0.05,|FC| ≥ 1.5), suggesting an involvement of fatty acid biosynthetic processing, G-protein coupled receptor protein signaling, and hormone activity, etc. in acupuncture-induced blood-pressure lowering., Conclusions: Twirling-reducing needling of acupuncture can lower blood pressure in prehypertension rats, which may be related to its effects in regulating expression of Acsm 3, Cgα, CryαB, Tshβ genes and in activating AITD pathway.

In order to improve the security of aircraft repaired structures, a method of crack propagation monitoring in repaired structures is put forward basing on characteristics of Fiber Bragg Grating (FBG) reflecting spectra in this article. With the cyclic loading effecting on repaired structure, cracks propagate, while non-uniform strain field appears nearby the tip of crack which leads to the FBG sensors' reflecting spectra deformations. The crack propagating can be monitored by extracting the characteristics of FBG sensors' reflecting spectral deformations. A finite element model (FEM) of the specimen is established. Meanwhile, the distributions of strains which are under the action of cracks of different angles and lengths are obtained. The characteristics, such as main peak wavelength shift, area of reflecting spectra, second and third peak value and so on, are extracted from the FBGs' reflecting spectral which are calculated by transfer matrix algorithm. An artificial neural network is built to act as the model between the characteristics of the reflecting spectral and the propagation of crack. As a result, the crack propagation of repaired structures is monitored accurately and the error of crack length is less than 0.5 mm, the error of crack angle is less than 5 degree. The accurately monitoring problem of crack propagation of repaired structures is solved by taking use of this method. It has important significance in aircrafts safety improvement and maintenance cost reducing.

Professor LI Zhi-dao's acupuncture technique at muscle belly and its clinical experiences for the treatment of arthralgia are introduced. From aspects of TCM, anatomical physiology and sports biomechanics, the theory basis of this technique is explained, while its method is elaborated in details from acupoint selection and manipulation combined with classic medical cases. The acupuncture technique at muscle belly is applied at the muscles which already have pathological changes, avoiding re-stimulation on the pain area. It provides new thinking methods and references for acupuncture to treat arthralgia.

Objective: To investigate the clinical significance of promoter methylation status of hPer3 gene in acute myeloid leukemia (AML) patients and the in vitro effect of decitabine (DCA) on AML cell lines HL-60 and U937., Methods: The promoter methylation status of hPer3 gene and mRNA expression levels in bone marrow of 206 AML and 40 iron deficiency anemia (IDA) patients (as control) were detected by methylation specific PCR (MS-PCR) and real-time PCR (RT-PCR). The HL-60 and U937 cell lines were treated with different concentrations of DCA for 48 and 72 h. The inhibition rates of cell proliferation were detected by methyl thiazolyl tetrazolium (MTT); the early apoptosis rates by staining with Annexin V and PI; the CD14 and CD11b expressions by flow cytometry (FCM); the promoter methylation status of hPer3 gene by MS-PCR; and the hPer3 mRNA expressions levels by RT-PCR., Results: The promoter methylation rates of hPer3 in newly diagnosed (ND) group, partial remission(PR) group, complete remission (CR) group, relapse (R) group and control group were 93.65% (59/63), 54.39% (31/57), 24.66% (18/73), 61.54% (8/13) and 0% (0/40), and the hPer3 mRNA expression levels were 0.19 ± 0.08, 6.28 ± 2.11, 52.76 ± 14.17, 8.18 ± 4.36, 75.03 ± 18.16, respectively. There was a significant statistic difference between any two group (P < 0.01) excepting for between PR and R group (P > 0.05). After DCA treatment, the promoter hypermethylation status of hPer3 was reduced and the mRNA and CD14, CD11b expression levels were up regulated in a dose dependent manner with an induction of cell apoptosis., Conclusions: Promotor methylation status and mRNA expression of hPer3 gene may be indicators for evaluating AML. DCA can induce the expression of hPer3 gene and cells apoptosis in AML.

Objective: To investigate the influence of Qingkailing (QKL) on learning and memory abilities, global neurotransmitter and phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway of senescence accelerated mouse-prone/8 (SAMP8) mice with Alzheimer's dementia (AD)., Methods: SAMP mice were modeled and divided into the model group, the QKL group and the doneppezil hydrochloride group, all treated for 90 days. And a control group was set up with senescence accelerated mouse-resistance/1 (SAMR1) mice. Morris water maze was used to test the learning and memory abilities of mice; contents of acetylcholine (Ach) and monoamine neurotransmitters in brain were measured by HPLC; levels of Grb2-associated binder-1 (Gab1), AKT and phospho-serine/threonine protein kinase B (PAKT473) were evaluated by Western-blot., Results: Compared with the control group, in the model group, the average escape latency detected by hidden platform trial and reverse trial on the 3rd day was higher (P < 0.01); levels of Ach, 5-hydroxytryptamine (5-HT) and Gab1 were lower (P < 0.01, P < 0.05 and P < 0.01), respectively. As compared with the model group, the escape latency (within the 2nd to 5th day) decreased (P < 0.01), levels of Ach and 5-HT increased (P < 0.05), and Gab1 protein expression increased (P < 0.01) in the QKL treated group after treatment, in addition, the level of phosphorylated AKT protein significantly increased (P < 0.05)., Conclusion: QKL could improve the learning and memory ability of AD model mice, which is probably related to its function in increasing cerebral Ach, 5-HT and activating PI3K/AKT pathway.

Objective: To investigate the imaging features of synovial tuberculosis of sheath of wrist., Methods: Three patients of synovial tuberculosis of sheath of wrist underwent surgical operation from Oct. 2002 to Oct. 2009 included 2 males and 1 female, the age of 48, 67, 76 years respectivly. X-rays of 3 patients, CTs of 2 patients and MRI of 1 patient were retrospectively analyzed and the relevant literature were reviewed., Results: There were 3 cases with the soft tissue mass in the palm side of wrist, the section was unclear. There were no osteoporosis and no changes of bone destruction. There was 1 case with the punctate calcification in the soft tissue. MRI showed the embedded cystic mass of flexor tendon and "8" shape in carpal tunnel pressure, and showed abnormal signal (T1 low-signal, T2 slightly higher signal), a small part of the internal point showed high signal. CT showed the synovial membrane were obvious thickening and enhanced, corpus liberum in tendon sheath were no obvious strengthening., Conclusion: Synovial tuberculosis of sheath of wrist has certain characteristics on radiographic image. The MRI has more clinical value than X-ray and CT.

Objective: To identify the mutation spectrum and the distribution of minihaplotypes (STR/VNTR) of phenylalanine hydroxylase (PAH) gene and explore the correlations between genotype and phenotype of patients with phenylketonuria (PKU) in Beijing area of China., Method: (1) Fifty cases with PKU were involved in this study. PKU was identified by the Neonatal Screening Center of Beijing. All 13 exons and their flanking intronic sequences of PAH gene of these patients were amplified and then subjected to SSCP analysis and direct sequencing. (2) The distribution of polymorphic locus of short tandem repeat (STR) and variable number tandem repeat (VNTR) was analyzed by PCR and denaturing gel electrophoresis. (3) The correlations between genotype and phenotype were studied by analysis of the matching rate between the expected and observed phenotypes. The predicted phenotype was determined on the basis of the sum of the assigned values of the two mutant alleles., Results: (1) A total of 34 different mutations were detected with the relative frequency of 95% among 50 PKU patients. The prevalent mutations in this study were: R243Q (20%), EX6-96A > G (11%), Y356X (9%), and V399V (7%). The next common mutations were R111X (5%), R413P (5%), R252Q (3%) and A434D (3%). Thirty-four detected mutations were distributed throughout the whole PAH gene, except exon 1, 8 and 13. Exon 7 and 11, with the mutant rate 34% and 19% respectively, seemed to be the hot mutant areas/regions of PAH gene. (2) The minihaplotypes (STR/VNTR) of 34 mutations were identified in this research. The STR and VNTR showed 8 and 3 alleles, respectively. Among them, 244 bp (44%) and 240 bp (34%) were the prevalent STR alleles. Meanwhile, the VNTR3 (83%) was the most common VNTR allele in PKU patients. (3) A better consistency (81.5%) between expected and observed phenotypes was revealed by analysis of correlation between genotype and phenotype. Especially in classic PKU, the consistency rate was up to 87.5%., Conclusion: (1) The frequency distribution of common PAH gene mutations in Beijing region was close to that of Tianjin and Yunnan regions, while it was different from that of Southern regions of China, such as Guangzhou, especially Taiwan. The PAH mutation with a highly heterogeneous trait was also demonstrated in this study. (2) STR and VNTR minihaplotype will prove helpful to trace the origins of PAH mutations and to analyze the genetic drift. However, the most minihaplotypes of the STR/VNTR are similar, so it is necessary to associate some other polymorphic loci with the STR/VNTR minihaplotype to analyze the different mutations. (3) The fact that a better consistency existed between phenotypes and genotype with most PKU patients suggested that the study of the genotype of PKU patients would be helpful to the individualized treatment and to genetic counseling for their families.

Aim: To study the mechanisms of sodium ferulate (SF) on inhibition of collagen synthesis in hepatic stellate cells., Methods: Collagen synthesis was analyzed by measuring 3H-proline incorporation. ELISA method was used to study the effect of SF on transforming growth factor beta1 (TGFbeta1) level in cultured HSC-T6 cell. The effect of SFon the TGFbeta1 activity in the supernatant of culture was analyzed by mink lung epithelial cell (Mv1Lu) proliferation inhibition by MTT assay., Results: SF inhibited collagen synthesis in hepatic stellate cells stimulated with TGFbeta1. SF was shown to decrease TGFbeta1 level in the supernatant of HSC-T6 increased by oxidative stress. TGFbeta1 activity was intervened by SF., Conclusion: SF could decrease collagen synthesis, with mechanism may be associated with that SF intervened TGFbeta1 activity, and reduced the level of TGFbeta1 increased by oxidative stress.

Aim: To study the effect of prothymosin alpha (Pro T alpha) as a fusion protein on secretion of IFN-gamma, IFN-alpha and TNF-alpha in vitro., Methods: The in vitro study was carried out on the culture of splenocytes, splenic and peritoneal macrophages isolated from Balb/c mice. Splenocytes were incubated with various concentrations of Pro T alpha (1 x 10(-7)-1 x 10(-10) mol.L-1) with or without Con A (5 micrograms.mL-1) for 72 h. Splenic and peritoneal macrophages were respectively treated with Pro T alpha (1 x 10(-7)-1 x 10(-10) mol.L-1) in the presence of LPS (10 micrograms.mL-1) for 24 h. Then IFN-gamma, IFN-alpha and TNF-alpha levels in the supernatant were detected by ELISA., Results: Pro T alpha (1 x 10(-7) mol.L-1) was found to obviously increase IFN-gamma level (P < 0.05) in the supernatant of splenocytes compared with the control group. Moreover, Pro T alpha (1 x 10(-7) mol.L-1) significantly induced the secretion of IFN-alpha (P < 0.01) and TNF-alpha (P < 0.01) in splenic and peritoneal macrophages., Conclusion: In vitro, Pro T alpha could increase the secretion of IFN-gamma, IFN-alpha and TNF-alpha.