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Objective: To develop a proper assay for identifying single nucleotide polymorphisms( SNPs) of the MGMT gene., Methods: PCR primers were designed by create restriction site (CRS) method, then polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was adopted to identify four SNPs in MGMT gene., Results: By PCR, one primer pair yielded target products containing MGMT84 SNP site, and the other primer pair yielded target products containing MGMT143, 160, 178 SNP sites. Four restriction enzymes were adopted to identify the four SNPs, respectively. The effects of PCR and RFLP were good., Conclusion: The methods for four SNPs of MGMT determinated by CRS-PCR-RFLP theory could be facility, economy, and rapidness.

Objective: To explore the relationship between genetic polymorphism of APE1, XRCC1 and susceptibility of chromosomal damage induced by vinyl chloride monomer (VCM)., Methods: 75 workers occupationally exposed to VCM were investigated. Chromosome damage in peripheral lymphocyte were measured by cytokinesis-block micronucleus (CB-MN) test. Created restriction site-restrained fragment length polymorphism (CRS-RFLP) and PCR-RFLP technique was applied to detect single nucleotide polymorphisms (SNPs) in APE1 gene (c.148) and XRCC1 gene (c. 194, c.280 and c. 399)., Results: The frequencies of the Asp and Glu alleles of APE1 148 in our study population were 61% and 39%, respectively. The frequencies of occurrence of the Arg and Trp alleles of XRCC1 194 were 85% and 15%. The frequencies of occurrence of the Arg and His alleles of XRCC1 280 were 85% and 15%, too. Whereas the prevalence of Arg allele was 87.0% and XRCC1 399 allele Gln was 13.0%. The risk of chromosomal damage induced by VCM for individuals carrying XRCC1 c. 194 Arg/Arg genotype was 0.6898 (95% CI 0.4997 - 0.9333, P = 0.0195) of those carrying Arg/Trp, Trp/Trp genotypes. The risk of chromosomal damage for female VCM exposure workers was 1.6000 (95% CI 1.2284 - 2.0699, P = 0.0004) of male workers. The haplotypes TGG/CGG and TGG/CAGCGGG were associated with the decreased frequencies of total micronucleus ( P < 0. 05)., Conclusion: It was suggested that female workers and subjects carrying XRCC1 194 Arg/Arg genotypes could be higher risk of chromosomal damage when they exposed to VCM.