1. [Effect of various methods of tissue storage on split-skin viability at 4 degrees C].
- Author
-
Jia X, Yang H, Liss K, McGann LE, and Tredget EE
- Subjects
- Animals, Cell Survival, Refrigeration standards, Skin cytology, Skin Physiological Phenomena, Swine, Time Factors, Tissue Preservation standards, Refrigeration methods, Skin metabolism, Tissue Preservation methods
- Abstract
Objective: To study various tissues and organs storage solutions (liver, kidney and corneal) at 4 degrees C for the storage of skin and the viability of skins stored in these solutions at correspondent condition., Method: Five experiment groups investigated for storing porcine split-skin at 4 degrees C were MEM, UW, MEM plus CS, UW-R and MEM-R. A new assay, WST-1, was applied to evaluate skin viability at 1, 3, 5, 7, 10 and 14 day during storage respectively., Result: Skin viability can all be protected by these three solutions. The effect of UW solution was superior to MEM. UW-R and MEM-R were superior to MEM and UW., Conclusion: The method of UW-R which replaces medium routinely may be an optimal method of storing skin at 4 degrees C.
- Published
- 1998