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Objective To observe the changes in invasion and migration abilities of lung adenocarcinoma cells with down-regulated histone acetyltransferase Tip60 and to investigate their underlying mechanisms. Methods The lung adenocarcinoma cell line A549 was divided into the Tip60 down-regulation group, negative control for down-regulation group, and control group. Cells in the Tip60 down-regulation group and the negative control for down-regulation group were transfected with Tip60-specific interfering siRNA and negative control siRNA, respectively, while cells in the control group were not transfected. The SETDB1 protein level in cells was detected using Western blotting, and the SETDB1 mRNA level was measured by real-time fluorescent quantitative PCR. Cell proliferation was assessed using the CCK-8 assay, cell invasion capability was evaluated using the Transwell chamber assay, and cell migration ability was observed using the wound healing assay. The significant enrichment peaks of histone H3 lysine 9 (H3K9) and lysine 27 (H3K27) were identified in the promoter region of SETDB1 in A549 lung adenocarcinoma cells by using the UCSC Browser feature of the Cistrome DB database. Chromatin immunoprecipitation (ChIP) was then performed to detect the acetylation levels of H3K9 and H3K27 in the promoter region of the SETDB1 gene. Results The expression levels of SETDB1 protein and mRNA in the Tip60 down-regulation group were lower than those in the control group and the negative control for down-regulation group (all P<0. 05) . At 24, 48, and 72 h of culture, the cell proliferation ability of the Tip60 down-regulation group was lower than those of the control group and the negative control for down-regulation group. The number of invasive cells was smaller in the Tip60 down-regulation group than in the control group and the negative control for down-regulation group. At 24 and 48 h of culture, the wound healing rate of the Tip60 down-regulation group was lower than those in the control group and the negative control for down-regulation group (all P<0. 05) . The acetylation levels of histone H3K9 and H3K27 in the SETDB1 promoter region were lower in the Tip60 down-regulation group than in the control group and the negative control for down-regulation group (all P<0. 05) . Conclusion Down-regulation of Tip60 can inhibit the invasion and migration abilities of lung adenocarcinoma cells, and the mechanism may be related to the regulation of acetylation levels of histone H3 in the SETDB1 promoter region. [ABSTRACT FROM AUTHOR]

Objective To observe the effect of methyltransferase SETDB1 on the migration and invasion of oral squamous cell carcinoma cells, and to explore the related mechanisms. Methods Different concentrations of methyltransferase inhibitors GSK3685032 (0，0. 2，0. 4，0. 6，0. 8 µmol/L) acted on the oral squamous cell carcinoma CAL-27 cells. Cell proliferative viability was measured by the MTT method, and we found that the optimal action concentration of GSK3685032 was 0. 6 µmol/L. CAL-27 cells were divided into the control and experimental groups; cells in the control group were treated with organic solvent DMSO, and cells in the experimental group were treated with 0. 6 µmol/L GSK3685032. Cell SETDB1 mRNA was measured by RT-qPCR, and the SETDB1 protein was detected by Western blotting. Cell migration ability was detected by Scratch assay, the invasion ability was detected by Transwell chamber invasion assay, and SOX 7 methylation level was tested by pyrosequencing. Results The mRNA and protein expression levels of SETDB1 were lower in the experimental group than in the control group (both P<0. 05). The migration distance of the experimental group was less than that of the control group, and the number of transmenbrance cells was smaller than that of the control group (P<0. 05). The methylation rate of SOX 7 in the experimental group was lower than that of the control group (P<0. 05). Conclusion Methyltransferase SETDB1 can inhibit the migration and invasion of oral squamous cell carcinoma cells, and the mechanism may be related to the down-regulation of intracellular SOX7 methylation level. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between the expression of Bcl-2-related transcription factor-1 (BCLAF1) and histone methylase SET domain branch type 1 (SETDB1) in hepatitis B-related hepatocellular carcinoma and clinicopathological features and prognosis after operation. Methods: 127 patients with hepatitis B-related hepatocellular carcinoma underwent radical resection in Zhujiang Hospital of Southern Medical University and Gaozhou People's Hospital from January 2018 to June 2020 were selected, the resected cancer tissues and adjacent tissues were taken, and the expression of BCLAF1 and SETDB1 were detected by immunohistochemistry, the relationship between the expression of BCLAF1 and SETDB1 and clinicopathological features were analyzed. 3 years after follow up, the survival differences of patients with different expressions of BCLAF1 and SETDB1 were analyzed by Kaplan-Meier survival curve, the factors affecting the prognosis of patients with hepatitis B-related hepatocellular carcinoma were analyzed by multivariate Cox regression analysis. Results: The positive expression rates of BCLAF1 and SETDB1 in cancer tissues were higher than those in adjacent tissues (P<0.05). The positive expression rates of BCLAF1 and SETDB1 in cancer tissues of patients with poor differentiation, TNM stage IIIa, portal vein invasion and lymph node metastasis were higher than those in cancer tissues of patients with moderate to high differentiation, TNM stage I~II, no portal vein invasion and no lymph node metastasis (P<0.05). The 3-year overall survival (OS) rate in HBV-related HCC patients with BCLAF1 positive expression and SETDB1 positive expression were lower than those in HBV-related HCC patients with BCLAF1 negative expression and SETDB1 negative expression (P<0.05). Multivariate Cox regression analysis showed that lymph node metastasis, BCLAF1 positive expression and SETDB1 positive expression were risk factors for poor prognosis in patients with hepatitis B-related hepatocellular carcinoma (P<0.05). Conclusion: The positive expression rates of BCLAF1 and SETDB1 in hepatitis B-related hepatocellular carcinoma tissues are increase, the positive expression of BCLAF1 and SETDB1 are relate to poor differentiation, TNM stage, portal vein invasion, lymph node metastasis and poor prognosis. [ABSTRACT FROM AUTHOR]