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Objective To investigate the relationship between the expression of SLIT3 and SPARCL1 protein and the recurrence of cervical cancer after radical operation. Methods From October 2019 to April 2021, 107 patients who underwent radical surgery for cervical cancer in the hospital were selected as the study subjects, and 30 patients with benign uterine lesions were selected as the control group ; The patients were followed up for 1 year and were divided into relapse group (n = 25) and non relapse group (n = 82) according to the follow-up results. The expression levels of SLIT3 and SPARCL1 mRNA were detected by real-time fluorescence quantitative analysis (qPCR), and the expression levels of SLIT3 and SPARCL1 protein were detected by immunohistochemistry. The correlation between the expression of SLIT3 and SPARCL1 protein and clinicopathological characteristics was analyzed. Cox model was used to analyze the risk factors of cervical cancer recurrence after radical operation. Results The expression of SLIT3 and SPARCL1 mRNA in cervical cancer group was significantly lower than that in control group (P < 0.05) ; The levels of SLIT3 and SPARCL1 mRNA in cervical cancer tissues of patients with recurrence were significantly lower than those of patients without recurrence (P < 0.05).Among 107 patients with radical operation, 25 patients recurred, the recurrence rate was 23.36%. The postoperative recurrence rate of patients with low expression of SLIT3 (31.15%) was significantly higher than that of patients with high expression of SLIT3 (13.04%). The postoperative recurrence rate of patients with low expression of SPARCL1 (32.31%) was significantly higher than that of patients with high expression of sparcli (9.52%). The difference was statistically significant (P < 0.05). The expression of SLIT3 and SPARCL1 in cervical cancer was correlated with the degree of differentiation, clinical stage, lymph node metastasis and depth of stromal invasion (P < 0.05), but not with age, pathological type and maximum diameter of tumor (P > 0.05 ). Cox univariate analysis showed that the degree of differentiation, clinical stage, lymph node metastasis, stromal invasion depth, SLIT3 protein expression and SPARCL1 protein expression were correlated with the recurrence of cervical cancer patients after radical surgery (P < 0.05 ) ; Cox multivariate analysis showed that moderate/high differentiation, stage IIA, lymph node metastasis, stromal invasion depth > 50%, low expression of SLIT3 protein and SPARCL1 protein were independent risk factors for recurrence of cervical cancer patients after radical surgery (P < 0.05). Conclusion the expression of SLIT3 and SPARCL1 in cervical cancer tissue is lower than that in normal cervical tissue. The low expression of SLIT3 and SPARCL1 protein is an independent risk factor for the recurrence of cervical cancer after radical operation, and has certain clinical significance in predicting the recurrence of cervical cancer after operation. [ABSTRACT FROM AUTHOR]

Objective:To analyze the influences of secreted protein acidic and rich in cysteines-like protein 1 (SPARCL1) on the proliferation,apoptosis and invasion of non-small cell lung cancer (NSCLC) cells,and to explore the role of Mitogen activation inhibitor(MEK)/Extracellular regulated protein kinase (ERK) pathway in it.Methods:From September 2019 to June 2021,the cancer tissues and corresponding adjacent tissues of 84 NSCLC patients who received surgical treatment in our hospital were collected,real time quantitative reverse transcription polymerase chain reaction (qRT-PCR) method was applied to measure and compare the expression level of SPARCL1 messenger RNA (m RNA) in tissues and normal lung epithelial cells HBEpiC and NSCLC cells A549,HCC827,H1299 and H292.A549 and HCC827 were selected for culture and grouping,they were divided into control group,NC si RNA group,SPARCL1si RNA group,U0126 group (MEK/ERK specific inhibitor),and SPARCL1 si RNA plus U0126 group.Cell counting kit 8 (CCK8) method and the plate cloning method were used to measure the proliferation of A549 and HCC827 cells,flow cytometry was applied to determine the apoptosis of A549 and HCC827 cells,Transwell chamber method was applied to determine the invasive ability of A549 and HCC827cells,Western blot was applied to detect the protein expression of SPARCL1,p-MEK,MEK,p-ERK1/2,ERK1/2.Results:The SPAR-CL1 m RNA expression level in NSCLC tissues was lower than that in adjacent tissues (P＜0.05).Compared with HBEpiC cells,the SPARCL1 m RNA expression level in NSCLC cells A549,HCC827,H1299 and H292 cells decreased (P＜0.05).Compared with the control group,the SPARCL1 m RNA expression level,protein expression and apoptosis rate of A549 and HCC827 cells in SPARCL1 si RNA group decreased (P＜0.05),the OD450,number of colonies formed,number of invasive cells,p-MEK/MEK,and p-ERK1/2/ERK1/2 protein expression increased (P＜0.05),the SPARCL1 m RNA expression level,protein expression and apoptosis rate of A549 and HCC827 cells in the U0126 group increased (P＜0.05),the OD450,number of colonies formed,number of invasive cells,p-MEK/MEK,and p-ERK1/2/ERK1/2 protein expression decreased(P＜0.05).Compared with the SPARCL1 si RNA group,the SPARCL1 m RNA expression level,protein expression and apoptosis rate of A549 and HCC827 cells in SPARCL1 si RNA plus U0126 group increased (P＜0.05),the OD450,number of colonies formed,number of invasive cells,p-MEK/MEK,and p-ERK1/2/ERK1/2 protein expression decreased (P＜0.05)Conclusion:SPARCL1 may affect the proliferation,invasion and apoptosis of A549 and HCC827 cells by regulating MEK/ERK pathway. [ABSTRACT FROM AUTHOR]