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At present，the most widely used control algorithms in the field of active noise control（ANC）are the classical FxLMS algorithm and its improved algorithms，which are applied to noise control in large spaces and areas such as the turboprop aircraft cabin，the amount of algorithmic calculations will be rapidly expanded with the increase in the number of channels of the ANC system，and the real-time requirements of algorithms are difficult to meet. Sequential Partial Update FxLMS（SPU-FxLMS）algorithm effectively solves this problem，but its convergence performance is weaker than that of the FxLMS algorithm. In this paper，the improvements to the SPU-FxLMS algorithm for the problem of slow convergence are made，so that the algorithm can be converged with a faster speed in the early stage of the operation，and then converge with a lower speed after converging to a smooth state. After converging to a steady state，the algorithm continues to run with a low computational capacity. The theoretical derivation and simulation analysis of the improved algorithm are carried out. The results show that the algorithm has good noise reduction performance and robustness while further reducing the amount of computation，and has a good prospect for engineering applications.

In liquidliquid extraction systems, extractants and extracted complexes may have surface activity, resulting in the formation of a variety of supramolecular assemblies such as vesicles and micelles. The assemblies are limited at microscopic and mesoscopic scales. Researches on interface of extraction also focus on microscopic and mesoscopic scales at present. Recently, our group first reported macroscopic supramolecular assembly(MSA) at the interface of extraction systems. The interface can be studied on macroscopic scales by MSA. The process of MSA in ionic liquids(ILs) based extraction systems also provides a new way to study complex supramolecular interactions between ILs and mediums. This review focused on microscopic, mesoscopic and macroscopic supramolecular assembly in extraction systems. First, the interface properties and the process of ion exchange in extraction were summarized. Next, the structures of mesoscopic supramolecular assemblies were reviewed . Finally, the macroscopic supramolecular assembly(MSA) at the interface of extraction systems and its application on separation were emphatically introduced. The MSA process at the interface enriches the extraction methods. And the Marangoni effect presented at the interface supplies a new approach to provide continuous driving force, which is an important scientific problem in the field of MSA.

The separation of strontium is important in the recovery of radioactive resources. Ionic liquids(ILs) based extraction systems of Sr have been greatly expanded, but the separation of Sr and Cs remains a problem. In addition, the problem of radioactive waste affects the application of IL systems in the field of spent fuel reprocessing. In this work, the macroscopic supramolecular assembly(MSA) of strontium at the interface of IL extraction systems and its basic research on the extraction of Sr were reported. The MSA behavior of Sr2+ in ionic liquid based extraction systems was studied and the structure of Sr-MSA was analyzed by mass spectrometry, 13C NMR, infrared spectroscopy and theoretical calculation. Sr-MSA was formed by using CnOHmimNTf2 (n=2, 3) as the ILs and CMPO, TODGA as the extractants. The three step mechanism of MSA process is proposed: complexes formed by Sr2+ and CMPO are extracted to IL phase. The complexes and IL assemble into nanoparticles driven by non covalent interactions such as electrostatic attraction, hydrogen, hydrophobic interaction. The nanoparticles aggregate into mesoscopic assemblies. The mesoscopic assemblies move to the interface and grow further into macroscopic intermediates. The intermediates finally generate into Sr-MSA under the traction from Marangoni effect. A new method for the selective extraction and one step solidification of Sr2+ from aqueous solutions was developed. The Sr-MSA can be taken out with tweezers easily from aqueous solution. The method can still be used in high acidity environment.

The coverage quality measuring and optimization problems were studied using moving target tracking as the application background. Based on the idea of grid discretization, a graph-theory based algorithm was proposed to search minimal exposure path for the moving target barrier coverage problem using exposure as the performance metric. And a multi-step incremental barrier coverage optimization method was proposed based on the minimal exposure path. The proposed algorithms’ performance was validated by simulation experiments in the end. Experiments show the algorithms are applicable to the barrier coverage problems with arbitrary node distribution model,sensing model,network charac- teristics and moving path of the target, can decrease the number of nodes need to be deployed in order to meet the specific coverage quality requirement and accordingly cut the network cost significantly.

Objective To investigate the expression of microRNA-142 (miR-142) in children with autoimmune thyroid disease (AITD) and its relationship with the imbalance of helper T cell 17 (Th17) and regulatory T cell (Treg). Methods A total of 89 children hospitalized for AITD from January 2019 to December 2022 were prospectively selected as the study subjects, including 48 children with Graves' disease (GD group) and 41 children with Hashimoto's thyroiditis (HT group). Additionally, 55 healthy children undergoing physical examinations during the same period were selected as the control group. The differences in serum miR-142, antithyroglobulin antibody (TGAb), antithyroperoxidase antibody (TPOAb), Th17/Treg, and interleukin-17 (IL-17) expression were compared among the groups. Results The expression of miR-142, TPOAb, TGAb, Th17, Th17/Treg, and IL-17 in the GD group and HT group was higher than that in the control group, while Treg was lower than that in the control group (P<0.05). Pearson correlation analysis revealed that in the GD group, miR-142 was positively correlated with TPOAb, TGAb, Th17, Th17/Treg, and IL-17 (r=0.711, 0.728, 0.785, 0.716, 0.709, respectively; P<0.001) and negatively correlated with Treg (r=-0.725, P<0.001); in the HT group, miR-142 was positively correlated with TPOAb and TGAb (r=0.752, 0.717, respectively; P<0.001). Conclusions miR-142 is highly expressed in children with AITD, and its expression may be related to the Th17/Treg imbalance in children with GD. [ABSTRACT FROM AUTHOR]

In this paper, Pickering emulsions with excellent stability was prepared by ultrafine pearl powder. The effects of pearl powder dosage, oil-water mass ratio and aqueous inorganic salt concentration on the formation and stability of Pickering emulsions were systematically investigated. The results show that the stability of the emulsion increases with the increase of the mass fraction of ultrafine pearl powder, and the preferred addition amount is 2.5%. Ultrafine pearl powder has the potential to prepare high internal phase Pickering emulsions with a maximum oil-water mass ratio of 8:2. With the increase of the oil-water mass ratio, the stability of the emulsion is firstly enhanced and then weakened, which reaches the highest centrifugal stability when the oil-water ratio is 4:6. The stability of the emulsion is not affected obviously when the concentration of sodium chloride in aqueous phase is low. The centrifugal stability of the emulsion increases when the concentration of sodium chloride is 20 mmol/L and decreases as sodium chloride concentration continues to increase. [ABSTRACT FROM AUTHOR]

This study is to investigate the influence of slurry viscosity on the diffusion and filling of slurry during grouting reinforcement. Firstly, based on the newly developed test device, the diffusion process of three kinds of viscosity slurry in the aquifer of confined aquifer was simulated, and the law of slurry diffusion and filling was summarized. Fluent was adopted to simulate the grouting process. The stress distribution and variation law during the filling process of different viscosity slurries was obtained, the abnormal phenomena appearing in the physical test process was explained, and the elastic modulus of the rock mass after different viscosity slurry transformation by ultrasonic measurement was obtained. The results show that: In the process of high-viscosity slurrygouting, the formation of high-stressed cracks in the region is irregularly developed, and the slurry diffusion is deflected to the right; Under the same stress, the elastic modulus(λ)of the rock block is proportional to the viscosity of the slurry(μe), and the λmax=108.07 % (μe=16.67)in the experiment; The medium viscosity slurry has a good transformation effect, and the area of the non-complete filling area is 23.35 %, and no abnormal phenomenon such as slurry accumulation occurs.

In order to study the stability and water isolation property of the boundary coal pillars after the mine is closed, FLAC3D numerical simulation software is used to the regularity of groundwater migration and water level rise as well as the variation of fault conductivity after the closure of the pit in Sanhejian Mine. The damage field and seepage field are numerically simulated, the seepage law under high water pressure is analyzed after the goaf is filled with water, the coupling between the surrounding rock damage field and the seepage field is evaluated. The results show that there is no overlapping conduction for the damage area of working face mining between Sanhejian Mine and Yaoqiao Mine, which meets the requirements for the safety of coal pillars. The development of seepage channels in mining rock masses is obviously affected by the mining of coal seams. Compared with non-conductive faults, the seepage field of water-conducting faults has a significantly larger scope. When the F19 fault is non-conductive and water-conducting, none of the plastic failure fields in the working face of Yaoqiao Coal Mine has penetrated the seepage field in the working face of Sanhejian Coal Mine, and the water isolation property of boundary coal pillars is good.

The modification design based on parametric gear model can improve the meshing performance rapidly and effectively. Parametric tooth profile and tooth helix modification of the fully parametric spur gear are carried out in the UG. The finite element simulation is performed by using Hypermesh and Workbench to obtain the gear meshing characteristics under different conditions. The simulation results show that for the same working condition,choosing the proper method of modification and modification parameters can obviously improve the meshing effect. At the same time,through the gear bench test,comparing the gear vibration data before and after modification,it is found that the gear vibration performance of the modified gear are obviously better than that of the unmodified gear. By analyzing the results of simulation and experiments,the modification of tooth axial of drum shape has a better effect on the partial load of tooth flanks and the profile modification has a significant effect on the improvement of load distribution between teeth. The optimum modification method and the amount of modification can quickly obtained by the parametric modification of gear which short the R & D cycle and provide an important theoretical reference for practical engineering.

To control vibration of support cantilever installed model in real time in wind tunnel, a support cantilever structure integrated with piezoceramics stacks, used as damping elements, is designed. And a neural network proportion-integration-differentiation (PID) real-time control method is proposed. The dynamics equation of the support cantilever is established, and the state equation for system identification is deduced through the neural network. After that, the controller is designed and realized by programming based on Labview software. Finally, the experiments in wind tunnel are implemented to validate the effectiveness of the controlling method. The experimental results indicate that real-time vibration reduction can be executed by the control system. For vibrations excited in wind tunnel at different wind speeds, the root mean square (RMS) amplitudes of vibrations are decreased by more than 55% using the control system in real time. The control method is possessed of robustness, reliability and fault-tolerance. [ABSTRACT FROM AUTHOR]

The vanguard novels in the mid 1980s differ from the previous ones on the Cultural Revolution in the narrative strategy and thematic orientation. These novels are no longer the confirmation and echo of the mainstream discourse and do not take revelation and criticism of negative sides in the society as their value orientation any more. In terms of narrative strategy, the Cultural Revolution, which was once the main plot and theme of macro history-styled narrative, has been transformed into the background of the novels and into a history of absurd distortions and deformations; a realist description of Culture Revolution has given way to an exploration of the psychological, allegorical and symbolic dimensions of the movement. In terms of the value orientation, the vanguard writers focus on the examination of the trauma and stress inflicted by the Cultural Revolution, and thus the cultural memory and trauma of a generation in a specific historical period has been constructed in the form of absurd carnival words and metaphorically polysemous texts. [ABSTRACT FROM AUTHOR]

For the application requirements of hyperelastic material silicone rubber in morphing wing skin sheet, the virtual work equation and the constitutive equation of large deformation with identical variables are presented. A finite element model of wing skin of rubber is developed, and its constitutive relation is simulated and validated. After that, its mechanics properties are calculated under different boundary conditions. The results indicate that, under the condition of fixed one end, the principle stress S11, S22 and principle strain E11, E22 are non-uniform distribution on the areas near the fixed end, but a large contraction strain is exhibited in y-direction. Moreover, its section thickness after deformation is also non-uniform. When another boundary condition of the displacement constraint in y-direction is imposed on the model, the distribution of S11, S22 and principle strain E11 represent uniform. The strain E22 is non-uniform on the small area near the loading end, and its value is very small. However, the thickness of deformed model presents uniform under this condition. [ABSTRACT FROM AUTHOR]

To restrain the forced vibration of a mechanical structure, a piezoelectric patch is pasted on the surface of the structure, and a resistance-negative capacitance shunted circuit is applied to piezoelectric passive control, then a fine vibration control effect can be gained by means of a suitable choice of the shunted circuit and its parameters. It is indicated by theoretical calculations and MATLAB simulation that on the premise of meeting the stable condition, a resistance-negative capacitance series shunted circuit is appropriate for the vibration control in low-frequency region, and a fine control effect can be gained when the resistance and the negative capacitance are a bit less. A resistance-negative capacitance parallel shunted circuit is appropriate for the vibration control in high-frequency region, and a fine control effect can be gained when the resistance and the negative capacitance are a bit more. In addition, a compensating circuit is designed. It can improve the conversion accuracy of negative capacitance and reduce Miller effect in the high-frequency region in parallel with the negative capacitance conversion circuit. [ABSTRACT FROM AUTHOR]

Using the high spatial resolution (2. 5 m) color-infrared aerial photos acquired in 1989, 1994, 2000 and 2005, this paper analyzed the spatiotemporal characteristics of rapid urban expansion in central Shanghai with urban expansion intensity index and gradient analysis. Results showed that urban land use in Shanghai increased rapidly in a "pancake" style during the study period, and the anisotropic urban expansion moved the urban center 2. 62 km toward southwest. The urban land use expansion intensity doubled and showed a rural-urban gradient. The most intensive urban expansion zone fell in the rural-urban transition zone, indicating the dominance of peripheral expansion as the primary urban expansion mode in Shanghai. However, the urban land use intensity decreased with time at the urban center. The primary driving forces of urban expansion included support from government policies and decision-making, enhanced economic activities, societal fixed assets investment, urban infrastructure investment, extension of transportation routes, as well as increase in urban population. [ABSTRACT FROM AUTHOR]

Yu Hua's publication of his Brother by installments in 2005 and 2006 is an important literary event in contemporary literature; the production and dissemination of the novel typically shows how the production and dissemination of literary works is dominated by the consumer market and commodity culture-led literary production mechanism. From the perspective of literary narrative, the consumer culture and its effect on literary production both influence the aesthetic and narrative dimensions of Brother, in which the history of "Cultural Revolution" and the social reality in the reform era both present a hyperbolic, caricatural and absurd tendency. All this indicates an invisible transformation of Yu Hua from a vanguard writer to a best-seller writer. Meanwhile, for the sake of the development of contemporary literature, we should be on the alert for consumer culture and try to avoid the possible negative effects it will exert on the autonomy of literature and literary aesthetic quality in the era when literature, market and consumer culture tie the knot. [ABSTRACT FROM AUTHOR]

In the contemporary childhood novels, the childhood motive has received rich and profound artistic representations and descriptions. From the perspective of narration, novels of this type usually contain the two tenses of "the past and the present" and the two perspectives of " the adults and the children" ; from the perspective of emotional tone, these novels are aesthetically and emotionally characterized by tenderness. In addition, the poetic retrospections of the childhood experience embody the authors' tracing and reflecting on the formation of their selves and contain a cultural awareness of how to explore the childhood psychology of mankind and the historical factors in the process of the formation of their own subjectivity. [ABSTRACT FROM AUTHOR]

The resistivity of the anti-corrosion coating of buried steel pipeline is an important parameter.In recent years，the value of this parameter has been used to evaluate the overall quality of the anti-corrosion coating of in-service or directional drilling pipelines.All the domestic standards regarding the on-site measurement of this parameter and the evaluation of the overall quality of the anti-corrosion coating are established with reference to NACE TM 0102-2002.However，those domestic standards have not explained the concept of "anti-corrosion coating resistivity" clearly and there is confusion with terms related to "anti-corrosion coating resistivity".The reasons for selecting the general method and the attenuation method are not defined clearly for the measurement of the resistivity of anti-corrosion coating in various standards，and the test location of the soil resistance is not specified when the general method is used for the directional drilling crossing pipeline，resulting in differences in the field test results and evaluation conclusions of the resistivity of the anti-corrosion coating outside the same pipeline by various inspection units.In order to clarify the above problems，the concepts related to the resistivity of the anti-corrosion coating were explained firstly，and then the meaning of the resistivity of the anti-corrosion coating was clarified through theoretical analysis and formula derivation.Furthermore，the measurement results of the resistivity of the anti-corrosion coating of 100 directional drilling crossing pipelines were statistically analyzed.Finally，suggestions on the revision of relevant standards were given，with expect to revise the unclear problems in the new version of the standards.

Objectives: To investigate the expression of microRNA-142 (miR-142) in children with autoimmune thyroid disease (AITD) and its relationship with the imbalance of helper T cell 17 (Th17) and regulatory T cell (Treg)., Methods: A total of 89 children hospitalized for AITD from January 2019 to December 2022 were prospectively selected as the study subjects, including 48 children with Graves' disease (GD group) and 41 children with Hashimoto's thyroiditis (HT group). Additionally, 55 healthy children undergoing physical examinations during the same period were selected as the control group. The differences in serum miR-142, antithyroglobulin antibody (TGAb), antithyroperoxidase antibody (TPOAb), Th17/Treg, and interleukin-17 (IL-17) expression were compared among the groups., Results: The expression of miR-142, TPOAb, TGAb, Th17, Th17/Treg, and IL-17 in the GD group and HT group was higher than that in the control group, while Treg was lower than that in the control group ( P <0.05). Pearson correlation analysis revealed that in the GD group, miR-142 was positively correlated with TPOAb, TGAb, Th17, Th17/Treg, and IL-17 ( r =0.711, 0.728, 0.785, 0.716, 0.709, respectively; P <0.001) and negatively correlated with Treg ( r =-0.725, P <0.001); in the HT group, miR-142 was positively correlated with TPOAb and TGAb ( r =0.752, 0.717, respectively; P <0.001)., Conclusions: miR-142 is highly expressed in children with AITD, and its expression may be related to the Th17/Treg imbalance in children with GD.

Objectives: Tongue squamous cell carcinoma (TSCC) is a common cancer in the oral and maxillofacial region, which seriously endangers people's life and health.Heterogeneous nuclear ribonucleoprotein A2/B1(hnRNP A2/B1) is an RNA-binding protein that regulates the expression of a variety of genes and participates in the occurrence and development of a variety of cancers. This study aims to investigate the role of hnRNP A2/B1 in TSCC progression., Methods: The differential expression of hnRNP A2/B1 in oral squamous cell carcinoma (OSCC) and normal oral mucosa cells and tissues was analyzed based on the gene expression profiles of GSE146483 and GSE85195 in the Gene Expression Omnibus (GEO) database. The correlation between hnRNP A2/B1 expression and disease-free survival of TSCC patients was analyzed based on TSCC related chip of GSE4676. TSCC cancer and paracancerous tissue samples of 30 patients were collected in Hunan Cancer Hospital from July to December 2021. Real-time RT-PCR and Western blotting were used to verify the mRNA and protein expression of hnRNP A2/B1 in TSCC patients'samples, respectively. Human TSCC Tca-8113 cells were transfected with hnRNP A2/B1 empty vector (a sh-NC group), knockdown plasmid (a sh-hnRNP A2/B1 group), empty vector overexpression plasmid (an OE-NC group) and overexpression plasmid (an OE-hnRNP A2/B1 group), respectively. The knockdown or overexpression efficiency of hnRNP A2/B1 was detected by Western blotting. The proliferation activity of Tca-8113 cells was detected by cell counting kit-8 (CCK-8), and the apoptosis rate of Tca-8113 cells was detected by flow cytometry., Results: Based on the analysis of OSCC-related chips of GSE146483 and GSE85195 in the GEO database, it was found that hnRNP A2/B1 was differentially expressed in the OSCC and normal oral mucosa cells and tissues (all P <0.01). Meanwhile, the analysis of TSCC related chip GSE4676 confirmed that the expression of hnRNP A2/B1 was negatively correlated with the disease-free survival of TSCC patients ( P =0.006). The results of real-time RT-PCR and Western blotting showed that the relative expression levels of hnRNP A2/B1 mRNA and protein in TSCC tissues were significantly up-regulated compared with those in adjacent tissues (all P <0.01). The results of Western blotting showed that the expression level of hnRNP A2/B1 in Tca-8113 cells was significantly inhibited or promoted after knockdown or overexpression of hnRNP A2/B1 (all P <0.01). The results of CCK-8 and flow cytometry showed that inhibition of hnRNP A2/B1 expression in Tca-8113 cells reduced cell proliferation activity ( P <0.05) and increased cell apoptic rate ( P <0.01). Overexpression of hnRNP A2/B1 in Tca-8113 cells significantly increased cell proliferation ( P <0.05) and decreased cell apoptosis ( P <0.01)., Conclusions: HnRNP A2/B1 is a key factor regulating the proliferation and apoptosis of TSCC cells. Inhibition of hnRNP A2/B1 expression can reduce the proliferation activity of TSCC cells and promote the apoptosis of TSCC cells.

Objective: To investigate the effect and relative mechanism of Recombinant Human Thrombopoietin (rhTPO) on long-term hematopoietic recovery in mice with acute radiation sickness., Methods: Mice were intramuscularly injected with rhTPO (100 μg/kg) 2 hours after total body irradiation with 60 Co γ-rays (6.5 Gy). Moreover, six months after irradiation, peripheral blood, hematopoietic stem cells (HSC) ratio, competitive transplantation survival rate and chimerization rate, senescence rate of c-kit + HSC, and p16 and p38 mRNA expression of c-kit + HSC were detected., Results: Six months after 6.5 Gy γ-ray irradiation, there were no differences in peripheral blood white blood cells, red blood cells, platelets, neutrophils and bone marrow nucleated cells in normal group, irradiated group and rhTPO group (P>0.05). The proportion of hematopoietic stem cells and multipotent progenitor cells in mice of irradiated group was significantly decreased after irradiation ( P <0.05), but there was no significant changes in rhTPO group (P>0.05). The counts of CFU-MK and BFU-E in irradiated group were significantly lower than that in normal group, and rhTPO group was higher than that of the irradiated group( P <0.05). The 70 day survival rate of recipient mice in normal group and rhTPO group was 100%, and all mice died in irradiation group. The senescence positive rates of c-kit + HSC in normal group, irradiation group and rhTPO group were 6.11%, 9.54% and 6.01%, respectively ( P <0.01). Compared with the normal group, the p16 and p38 mRNA expression of c-kit + HSC in the irradiated mice were significantly increased ( P <0.01), and it was markedly decreased after rhTPO administration ( P <0.01)., Conclusion: The hematopoietic function of mice is still decreased 6 months after 6.5 Gy γ-ray irradiation, suggesting that there may be long-term damage. High-dose administration of rhTPO in the treatment of acute radiation sickness can reduce the senescence of HSC through p38-p16 pathway and improve the long-term damage of hematopoietic function in mice with acute radiation sickness.

Objective: To confirm the therapeutic effect of recombinant human thrombopoietin (rhTPO) on rhesus monkeys irradiated with 5.0 Gy 60 Co γ-ray, and provide experimental basis for clinical treatment of similar patients., Methods: Fourteen adult rhesus monkeys were irradiated with 60 Co γ-ray on both sides at the dose of 5.0 Gy (dose rate 69.2 cGy/min) to establish the acute radiation sickness model. The monkeys were divided into irradiation group (n=5), rhTPO 5 μg/kg group (n=4) and rhTPO 10 μg/kg group (n=5). Two hours after irradiation, the three groups of monkeys were injected with saline 0.1 ml/kg, rhTPO 5 μg/kg（0.1 ml/kg） and rhTPO 10 μg/kg(0.2 ml/kg), respectively. The general signs, survival, peripheral hemogram and serum biochemistry of rhesus monkeys were observed before and after irradiation, and the differences between rhTPO group and irradiation control group were compared., Results: After total body irradiation with 5.0 Gy 60 Co γ-ray, rhesus monkeys successively showed fever, hemorrhage, sharp decrease of whole blood cell counts in peripheral blood and disorder of serum biochemical indexes. Compared with the irradiated control group, a single intramuscular injection of rhTPO 5 μg/kg or 10 μg/kg 2 hours after irradiation could improve the symptoms of fever and bleeding, increase the nadir of peripheral red blood cells and platelets counts, shorten the duration of hemocytopenia, and advance the time for blood cells to return to the pre-irradiation level. The serum biochemical results showed that rhTPO could improve the abnormality of serum biochemical indexes in rhesus monkeys induced by 5.0 Gy total body irradiation to some extent. Compared with the two administration groups, the therapeutic effect of rhTPO 10 μg/ kg was better., Conclusion: A single injection of rhTPO 5 μg/ kg or 10 μg/ kg 2 hours after irradiation can alleviate the injury of multilineage hematopoiesis and promote the recovery in monkeys irradiated by 5.0 Gy γ-ray. It also improves animal signs and has obvious therapeutic effect on acute radiation sickness.

Objective: To investigate the effect of atovaquone on the cell cycle and apoptosis of non-Hodgkin's lymphoma Raji cells, and clarify the related mechanisms., Methods: MTT assay and trypan blue dye exclusion method were used to evaluate the effect of atovaquone on the proliferation of Raji cells. After the cells were stained by PI staining, the cell cycle distribution was detected by flow cytometry. Cell apoptosis was analyzed by Annexin V/PI double binding assay. The intracellular alterations of reactive oxygen species were detected by 2', 7'-dichlorofluorescein diacetate (DCFH-DA). The protein expression of cell cycle and apoptosis related molecules were detected by Western blot., Results: Various concentrations of atovaquone (5-40 μmol/L) inhibited the growth of Raji cells in a concentration-dependent manner ( r =0.951). The proliferation of Raji cells was significantly inhibited after treated by atovaquone (20 and 30 μmol/L) for 24, 48 and 72 h, which showed statistically different with that in the control group ( P <0.01, P <0.001, P <0.001). G 1 phase arrest ( P <0.01, P <0.001) and apoptosis ( P <0.01) of Raji cells was induced by atovaquone (20 and 30 μmol/L) significantly for 24 h and 48 h, respectively. The expression of p-JAK2 and p-STAT3(Y705) protein were down-regulated significantly induced by atovaquone ( P <0.001, P <0.05). Furthermore, atovaquone treatment could induce the decreasing of antiapoptotic protein Mcl-1, Bcl-2, and Bcl-xl expression level ( P <0.05) and increasing of cleaved caspase-3 protein expression level. In addition, atovaquone could also induce the down-regulation of c-Myc ( P <0.001, P <0.01) and cell cycle related molecules Cyclin D1, CDK4, and CDK6 ( P <0.01, P <0.05) protein expression., Conclusion: Atovaquone effectively inhibits cell proliferation and induces cell cycle arrest and apoptosis by suppression of STAT3 signaling pathway in Raji cells. It can be a potential therapeutic agent against non-Hodgkin's lymphoma.

Objective: To study the effect of interleukin-6 (IL-6) gene deletion on radiation-induced hematopoietic injury in mice and relative mechanism., Methods: Before and after whole body 60 Co γ-ray irradiation, it was analyzed and compared that the difference of peripheral hemogram, bone marrow hematopoietic stem and progenitor cells conts in IL-6 gene knockout (IL-6 -/- ) and wild-type (IL-6 +/+ ) mice and serum IL-6 and G-CSF expression levels in above- mentioned mouse were detected. Moreover, 30 days survival rate of IL-6 -/- and IL-6 +/+ mice after 8.0 Gy γ-ray irradiation were analyzed., Results: IL-6 levels in serum of IL-6 +/+ and IL-6 -/- mice were respectively (98.95±3.85) pg/ml and (18.36±5.61) pg/ml, which showed a significant statistical differences (P<0.001). There were no significant differences of peripheral blood cell counts and G-CSF level in serum between IL-6 +/+ and IL-6 -/- mice before irradiation (P>0.05). However, the number of leukocytes, neutrophils, lymphocytes, monocytes, platelets in peripheral blood and G-CSF level in serum of IL-6 -/- mice were significantly decreased at 6 h after 8.0 Gy γ-ray irradiation compared with that of IL-6 +/+ mice. On days 30 after 8.0 Gy γ-ray irradiation, the survival rate of IL-6 +/+ and IL-6 -/- mice was 62.5% and 12.5%, and the mean survival time of dead mice was 16.0±1.0 and 10.6±5.3 days, respectively. On days 14 after 6.5 Gy γ-ray irradiation, bone marrow nucleated cells in IL-6 +/+ and IL-6 -/- mice were respectively (10.0±1.2)×10 6 and (8.3±2.2)×10 6 per femur. Compared with IL-6 +/+ mice, the proportion of Lin - Sca-1 - c-kit + (LK) in bone marrow of IL-6 -/- mice had no significant change (P>0.05), but the proportion of Lin - Sca-1 + c-kit + (LSK) was significantly decreased (P<0.05)., Conclusion: IL-6 plays an obvious role in regulating hematopoietic radiation injury, and IL-6 deficiency can inhibit the radiation-induced increase of endogenous G-CSF level in serum, aggravates the damage of mouse hematopoietic stem cells（HSC） and the reduction of mature blood cells in peripheral blood caused by ionizing irradiation, resulting in the shortening of the survival time and significant decrease of the survival rate of mice exposed to lethal dose radiation.

Objective: To establish a leukemia mouse model induced by transplantation of hematopoietic cells from mixed lineage leukemia (MLL)-AF9 transgenic mice so as to provide the basis for the mechanism research and drug screening of acute myeloid leukemia (AML)., Methods: MLL-AF9 knock-in mice were bred and identified. When the mice developed leukemia, white blood cell (WBC) count in peripheral blood, flow cytometry and morphology method were analyzed to identify the disease. When the WBC count in peripheral blood was more than 100×10 9 /L, bone marrow cells and spleen cells were collected and cryopresevated. After resuscitation, the cells were injected into 4.5 Gy irradiated wild C57BL/6J mice through the tail vein to develop MLL-AF9 leukemia mouse model. Finally, the therapeutic effect was evaluated by positive drug on the model., Results: The natural onset times of leukemia on MLL-AF9 knock-in mice were 22-28 weeks. The spleens of the transgenic mice enlarged and the bone marrow showed the immature forms of myeloid leukemia cells. Both the bone marrow and spleen cells highly expressed myeloid markers, CD11b and Gr-1. At least 0.5×10 6 bone marrow cells and 2.5×10 6 spleen cells could induce leukemia in all recipient mice, and the median survival times of mice were 20 days and 36 days, respectively. Experimental treatment was carried out on the leukemia mouse model transplanted with MLL-AF9 spleen cells, and it was found that the traditional chemotherapy drug cytarabine could delay the onset of leukemia and prolong the survival time of the mouse model., Conclusion: The leukemia model of hematopoietic cell transplantation based on MLL-AF9 transgenic mice is successfully established, which can be used for the study of the pathogenesis and evaluation of therapeutic effect of AML.

N 6 -methyladenosine (m 6 A) is a prevalent modification of RNA in eukaryotes and plays an important role in the process of mRNA translocation, stabilization and translation. m 6 A exerts different influences on the viral replication cycle, and both viral replication and host immune response to the virus are affected by m 6 A. In this review, we summarize recent studies on the mechanism of m 6 A modification and its effects on viral replication and host immune response, in order to provide a reference for epigenetic regulation in the viral life cycle.

Objective: To study the therapeutic effect of rhSCF early administration on rhesus monkeys with severe acute radiation sickness(ARS)., Methods: Twelve adult monkeys totally exposed to 7.0 Gy 60 Co were divided into radiation control and SCF groups, and monkeys in SCF group were subcutaneously injected recombinant human SCF(rhSCF) 200 µg/kg at half an hour and 24 hour after irradiation, while the radiation control monkeys were injected physiological saline. Survival was monitored and hematopoiesis was evaluated at 40 days following early treatment., Results: 6 animals treated with rhSCF all survived, while 2 in irradiated controls survived on 40 day after radiation. rhSCF treatment promoted hematopoiesis recovery significantly, increased the nadir of white blood cells, neutrophils and platelets, and simplified supportive care in ARS rhesus monkeys., Conclusion: RhSCF injection soon after TBI taken shows an significant therapeutic efficiency on rhesus monkeys with severe acute radiation sickness.

Objective: To evaluate the therapeutic effects of combined administration of recombinant human granulocyte colony-stimulating factor (rhG-CSF), recombinant human thrombopoietin (rhTPO) and recombinant human interleukin-2 (rhIL-2) on radiation-induced severe haemopoietic acute radiation sickness (ARS) in rhesus monkeys, so as to provide experimental evidences for the effective clinical treatment., Methods: Seventeen rhesus monkeys were exposed to 7.0 Gy (60)Co γ-ray total body irradiation (TBI) to establish severe haemopoietic ARS model, and were randomly divided into supportive care group, rhG-CSF+rhTPO treatment group and rhG-CSF+rhTPO+rhIL-2 treatment group. Survival time, general signs such as bleeding and infections, and peripheral blood cell counts in each group were monitored. Bone marrow cells were cultivated to examine the colony formation ability. The histomorphology changes of bone marrow were observed at 45 d post irradiation., Results: After 7.0 Gy (60)Co γ-ray TBI, monkeys of supportive care group underwent tarry stool and emesis, then died in 12~18 d. The overall survival rate in this group was 16.7%. Gastrointestinal reactions of monkeys in two combined-cytokines treatment groups were inapparent. Combined-cytokines treatment induced 100% survival. Complete blood cells declined sharply after irradiation in each group, but two combined-cytokines treatment schemes could elevate the nadir of all blood cells, shorten the duration of pancytopenia and accelerate the recovery of hemogram. Compared with rhG-CSF+ rhTPO treatment, rhG-CSF+ rhTPO+ rhIL-2 treatment could increase the counts of lymphocytes and monocytes. The colony-formation rate of haemopoietic stem/progenitor cells in bone marrow dropped markedly at 2 d after irradiation. Combined-cytokines treatment promoted the ability of colony formation on day 29. Hematopoietic cells mostly disappeared in bone marrow of animals in supportive care group, but hematopoietic functions were recovered after cytokines were administrated., Conclusion: rhG-CSF+ rhTPO and rhG-CSF+ rhTPO+ rhIL-2 treatment can significantly promote hematopoiesis recovery, improve the quantity of life, simplify the supportive therapy, and enhance the survival rate of rhesus monkeys with severe haemopoietic ARS induced by 7.0 Gy (60)Co γ-ray exposure. Especially the application of rhIL-2 can accelerate the recovery of lymphocytes and monocytes and restore the immunological function. Thus, combination of rhG-CSF, rhTPO and rhIL-2 on the basis of supportive care is an efficient strategy to treat severe haemopoietic ARS.

Objective: To investigate the effects of HS 6101 and recombinant human granulocyte colony stimulating factor (rhG-CSF) on hematopoiesis recovery of ICR mice injured by cyclophosphamide (CTX)., Methods: A total of 103 ICR mice were divided into 4 groups, including CTX control (46 mice), HS 6101 (21 mice), rhG-CSF (18 mice) and HS 6101+rhG-CSF (18 mice), respectively. The mouse model of chemotherapy-induced haematopoietic injury was established by CTX intraperitoneal injection at a dose of 100 mg/kg once a day for 3 consecutive days. Single dose of HS 6101 (27 µg/mouse) was injected subcutaneously at 1 hour before the first administration of CTX. One day after the last CTX treatment, 2 µg/mouse of rhG-CSF was injected subcutaneously once a day for 5 consecutive days. The peripheral blood cell counts of the mice were observed once every 2 days. Hematopoietic progenitor cell colony counting and histopathological assessment of bone marrow cells were evaluated in the mice at days 4 and 9 after the first administration of CTX., Results: Both HS 6101 and rhG-CSF alone or in combination, significantly elevated the nadirs of peripheral blood leukocytes and neutrophils, increased the number of bone marrow hematopoietic progenitor cells, and stimulated the hematopoietic cell hyperplasia of bone marrow in the mice treated with CTX. The effect of HS 6101 combined with rhG-CSF was better than that of the drugs used alone., Conclusion: The HS 6101 at 27 µg/mouse can significantly promote the recovery of hematopoiesis in ICR mice treated with CTX chemotherapy, and its combination with rhG-CSF shows synergistic effects.

Objective: To explore the preventive effect of Yougui drink on femoral head necrosis in rats under micro CT., Methods: Twenty-five SD rats were divided into steroid hormone group (group A, 10 rats ), Yougui drink group (group B,10 rats) and normal group (group C,5 rats)with random number table. Endotoxin were injected into abdominal cavity of rats in group A and B for 2 days, methylprednisolone sodium succinate were injected by gluteus for twice a week continued for 6 weeks; group B were gavaged by Yougui drink (veryday for 8 weeks; group C did not do any processing. All rats were killed on the 10th weeks,m icro CT were used to scan femoral head in vitro and preventive effect of Yougui drink (n femoral head necrosis in rats., Results: There was statistical significance in BMD, BV/TV, Tb.N, Tb, Th, Thb, Sp, BS/TV and DA but no significance in SMI between group A and B. Comparison between A and C, there was significant meaning in BMD, BV/TV, Tb.N, Tb, Th, Tb, Sp, BS/TV, DA and SMI., Conclusion: Yougui drink on femoral head necrosis in rats under micro CT has preventive effect from BMD BV/TV, Tb.N, Tb, Th, Tb, Sp, BS/TV and DA.

Objective: To investigate effective doses of cyclophosphamide (CTX) for establishment of leukopenia model in ICR mouse., Methods: Normal ICR mice (n = 96) were divided into CTX(80), CTX(100), CTX(120), CTX(150) and CTX(300) groups, of which mice received CTX intraperitoneally at a dose of 80, 100, 120, or 150 mg/kg once a day for 3 consecutive days, or 300 mg/kg with single injection. The peripheral blood cell counts were detected at various times before and after CTX administration., Results: The peripheral white blood cell nadirs in the mice injected with CTX appeared on day 4 after the first dose of CTX. The peripheral white blood cell nadir in group CTX(100) was 26.7% of the value measured in mice before CTX administrated, and that of group CTX(80) was 35.0%. Higher doses of CTX, however, caused too severity in hematopoietic injury., Conclusion: The dose of CTX 100 mg/(kg·d) × 3d is appropriate for leucopenia model of ICR mouse.

Objective: This study was aimed to investigate the mobilization effect of HS6101 on hematopoietic cells of mice., Methods: The normal ICR mice were injected subcutaneously once or twice with HS6101 at 9 µg/d/mouse, or a single dose of HS6101 3, 9, 27 and 81 µg/mouse was administrated, and the mobilization effect of HS6101 in different administration times and different dosage was observed, and compared with the synergistic effects of administration of single dose of HS6101 combined with rhG-CSF (2 µg/d/mouse was injected subcutaneously for 5 consecutive days). The peripheral blood cell counts of mice were detected at different time after administration. The hematopoietic stem/progenitor cells of bone marrow and peripheral blood were detected at day 5 and 10 after administration., Results: There was no significant difference in peripheral blood cell counts after once or twice injections of HS6101 9 µg/mouse. The peripheral platelet counts dose-dependently increased in ICR mice, which accounted for 121.1% to 118.0%, 138.7% to 123.1%, 146.4% to 139.2%, and 156.2% to 168.7% (P < 0.001) after HS6101 (3, 9, 27 and 81 µg/mouse) treatments at 5 and 7 d, respectively. HS6101 (3, 9, 27 and 81 µg/mouse) showed dose-response relationship to platelets, with R value of 0.777 and 0.954 at day 5 and 7 after administration, respectively. HS6101 significantly increased numbers of hematopoietic stem/progenitor cells in both bone marrow and peripheral blood, and elevated peripheral blood leukocytes at 27 µg/mouse dose at day 5 after administration., Conclusion: HS6101 has significant mobilization effect on hematopoietic stem/progenitor cells, platelets and leukocytes in mouse.

This study was purposed to investigate the protective effects of lipoprotein HS-6101(6101) on rhesus monkey total body irradiated with 7.0 Gy ⁶⁰Coγ-ray. A total of 30 health adult rhesus monkeys were randomly divided into symptomatic therapy (ST), WR2721 and HS-6101 30, 90 and 270 mg/kg groups (n = 6), the rhesus monkeys of each groups were injected with physiological saline 0.3 ml/kg, WR-2721 30 mg/kg, or HS-6101 30, 90 and 270 µg/kg, respectively. All agents were once intramuscularly injected at 1 hr prior irradiation. General observation, peripheral blood cell counts, colony forming unite assay of bone marrow hemopoietic progenitor cells, and histopathological examination were performed. The results showed that animals in symptomatic therapy group begin to die on the 13(th) day and 4 animals died within 24 days, the average survival time was 18.2 ± 4.3 days; 2 animals in WR-2717 groups died on day 15.8 and day 18.5 post irradiation respectively. 1 animal in HS-6101 270 mg/kg group died on day 35.8, all other animals survived. Nadirs of peripheral blood white blood cells, neutrophils and platelets of animals in HS-6101 treatment groups were significantly higher than those in other 2 groups including ST and WR-2721 groups, and the hemopoietic recovery were also significantly speeding up(P < 0.05 and 0.01). In vitro results showed that HS-6101 obviously promoted 7.0 Gy ⁶⁰Coγ irradiated monkey's bone marrow mononuclear cells to form various hematopoietic progenitor cell colonies (P < 0.05 and 0.01) . Compared with symptomatic therapy and WR-2717 groups, bone marrow histopathological changes in HS-6101 treatment groups showed more active hemopoietic cell proliferation and higher density structure. It is concluded that HS-6101 90 µg/kg treatment can promote the bone marrow recovery of 7.0 Gy ⁶⁰Coγ irradiated monkey, alleviate their animal symptom, simplify the treatment measures and improve the animal survival rate. The HS-6101 shows remarkable radioprotective effects as compared with the currently internationally acknowledged radioprotectant of WR-2721.

Objective: To review the clinical manifestations and management of nasal sinus mucoceles invaded the skull base and orbit., Method: Medical records for 30 patients of paranasal sinus mucoceles invaded the skull base and orbit were reviewed retrospectively. CT and MRI were important tools for diagnosing nasal sinus mucocele. The patients received endoscopic surgery to remove mucocele, some of them were operated auxiliary incision. Steroid therapies were given after the operations and routine examination with endoscopy were carried out during follow-up., Result: All cases were successfully performed surgery without complications after the surgeries, or the majority of symptoms. However, vision recovery was observed only in some patients. Recovery of vision depended on the timing of surgery and severity of initial visual loss. Delay treatment can seriously compromise the recovery of vision impairment. Moreover, the patients without light perception before surgery had poor visual recovery even when optic nerve decompressions were performed., Conclusion: Endoscopic surgery has effect on nasal sinus mucocele with visual loss. Because visual recovery depends on prompt diagnosis and surgical intervention,a good understanding of the disease and prompt imaging studies are important.

Using the high spatial resolution (2.5 m) color-infrared aerial photos acquired in 1989, 1994, 2000 and 2005, this paper analyzed the spatiotemporal characteristics of rapid urban expansion in central Shanghai with urban expansion intensity index and gradient analysis. Results showed that urban land use in Shanghai increased rapidly in a "pancake" style during the study period, and the anisotropic urban expansion moved the urban center 2.62 km toward southwest. The urban land use expansion intensity doubled and showed a rural-urban gradient. The most intensive urban expansion zone fell in the rural-urban transition zone, indicating the dominance of peripheral expansion as the primary urban expansion mode in Shanghai. However, the urban land use intensity decreased with time at the urban center. The primary driving forces of urban expansion included support from government policies and decision-making, enhanced economic activities, societal fixed assets investment, urban infrastructure investment, extension of transportation routes, as well as increase in urban population.

This study was aimed to investigate the biological effects of Rhesus bone marrow mesenchymal stem cells (R-BMMSC) transfected by adenovirus bearing extracellular superoxide dismutase gene (AD-ECSOD). Using density gradient centrifugation and adherent culture way, the R-BMMSC transfected by AD-ECSOD and reporter gene EGFP were isolated, cultured and purified; the transfection efficiency was detected by fluorescence microscopy and flow cytometry; the ECSOD protein expression in cell culture supernatant were detected by ELISA; the surface antigens on R-BMMSC (CD34, CD29, CD45, CD90, HLA-DR) were detected by flow cytometry; and differentiation capability of transfected R-BMMSC were detected by oil red O and alizarin staining; the proliferation capability of R-BMMSC was assay by MTT method. The results showed that the transfection efficiency of AD-ECSOD (MOI 500, 1 000, 1 500 and 2 000) for R-BMMSC was > 95%. At 24 h after transfection, the ECSOD protein could be detected in cell culture supernatant, and its level was significantly higher than that of control group (P < 0.01). At 48 h after transfection, the expression level of ECSOD protein on MOI 1 500 and 2 000 was the highest. The proliferative capability, surface antigen expression and multi directive differentiation ability of transfected R-BMMSC were similar to non-transfected R-BMMSC. It is concluded that the AD-ECSOD can effectively transfect the R-BMMSC without influences on its biological features.

This study was aimed to investigate the radioprotective effects of recombinant human interleukin-12 (rhIL-12) on monkey hematopoietic system, and to provide experimental evidence for future clinical prophylaxis and treatment for patients who suffered from acute radiation syndrome. In in vitro study, the effect of rhIL-12 in different concentrations (0, 1, 5, 25, 125 and 625 ng/ml) on colony forming capacity of human or monkey bone marrow-derived mononuclear cells was examined in methylcellulose H4434 medium. In in vivo study, the acute radiation syndrome model was established in 11 Rhesus monkeys which received lethal total body irradiation by 6 Gy (60)Co γ in single time irradiation. The irradiated monkeys were randomly divided into 3 subgroups: control group (n = 4) which received subcutaneous PBS injection, rhIL-12 single-dose group (n = 3) which received subcutaneous single injection of rhIL-12 (4 µg/kg) at 2 h after irradiation, and multiple-dose group (n = 4) which received subcutaneous injection of rhIL-12 (1 µg/kg per injection) at 2 h, day 3, 6 and 9 after irradiation respectively. Peripheral blood cells were counted before and after irradiation every other day. The survival status of animals were observed daily. In vitro test results showed that different concentrations of rhIL-12 obviously promoted human and healthy monkeys' bone marrow mononuclear cells to form various hematopoietic progenitor cell colonies, especial CFU-E and CFU-GM. All animals in control group died within 22 d after lethal total body irradiation, average survival time was (20.3 ± 1.2) d. Only one monkey in multiple-dose group died due to anemia on day 17. All monkeys in single-dose group survived. Compared with control group, rhIL-12-administrated monkeys' white blood cell count, hemoglobin level, platelet and reticulocyte counts showed faster recovery from high dose radiation. It is concluded that the rhIL-12 treatment can promote the bone marrow hematopoietic stem/progenitor cell colony formation in vitro and protect lethally-irradiated monkeys. There is an obvious therapeutic effect of rhIL-12 on monkeys suffered from bone marrow failure caused by severe acute radiation exposure.

Objective: To understand the abdominal CT manifestation and its pathology of patients with chronic schistosomiasis., Methods: The plain + enhanced CT scanning was performed in 42 patients with chronic schistosomiasis, and their tissues of biopsy and surgical resection were examined pathologically., Results: Among the 42 patients, schistosomiasis cirrhosis of liver was found in 36 cases, left lobe enlarged was found in 21 cases, and calcification was found in 32 cases. Portal vein and its tributaries calcification were found in 14 patients, and one of them was accompanied with transplanted hepatocellular carcinoma, 12 patients with stones in gallbladder, and 5 patients with ascites. Among the 19 patients with splenomegaly, 7 patients were accompanied with calcification. Intestinal wall thickening with linear and tram-like calcification was seen in 21 cases. Descending colon, sigmoidcolon, and rectum calcifications were found in 8 cases, and rectosigmoid calcification was found in 9 cases, rectum calcification was seen in 2 cases, and 2 patients were accompanied with colon carcinoma. The pathological examination showed fibrosis and calcified ova in liver, large intestine and gallbladder of the patients, but there were no ova in spleen., Conclusion: Abdominal CT manifestation of patients with chronic schistosomiasis presents the damage of multiple organs in abdomen, and various calcifications are the main image characteristics.

Objective: To identify the epidemic characteristics and risk factors of an emerging infectious disease-severe fever with thrombocytopenia syndrome (SFTS) in Hubei province., Methods: Active surveillance program on SFTS was set up in monitoring sites-hospitals, at the township level or above, in Suizhou, Huanggang and Wuhan from January to December, 2010. Specific surveillance program on SFTS was launched across the province in hospitals above the county level. Cases that matched the definition of surveillance case were identified and reported to Centers for Disease Control and Prevention (CDCs). Cases were interviewed and their blood samples collected and detected using PCR and virus isolation. We also conducted serum antibody surveys among healthy population and livestock and surveillance on vector ticks in those high-epidemic areas., Results: 188 cases that matched the definition of surveillance case and 21 deaths were reported in 11 cities, 32 countries and 100 towns in 2010, with an incidence rate of 0.33/10(6). The fatality rate was 11.2%. Data showed that the patients were from hilly areas at the altitude elevated between 28-940 meters. The epidemic period was between April and December with the peak from May to September. The youngest case was an 11-year old, while the eldest was 81 with median age as 56-year old. 95.3% of the patients were farmers. All Patients did not have the history of traveling, two weeks before the onset of SFTS. 93.6% of the patients engaged in different kind of work which was associated with agriculture. 52.8% of the patients had been exposed to ticks. 22.0% of the patients had been bitten by ticks. Skin injury was found in 64.2% of the patients. Samples from 129 cases (68.6%) were collected and detected, with 67.4% of them (87 cases) showed positive by Real time-PCR for SFTS virus. An elevation in antibody titer by a factor of four or evidence of sero-conversion was observed in 11 patients; SFTS virus was isolated from 2 patients. The total antibody positive rates were 3.8%, 55.0% (6/11), 36.7% (2/3) and 80.0% (4/5) respectively in healthy population, dogs, sheep and cows. Ticks from grass, cattle and sheep were detected positive by Real time-PCR., Conclusion: Most cases of SFTS in Hubei were infected by SFTS virus, and cases of livestock were infected by SFTS virus. Ticks might serve as an important vector. Skin injury, exposure to tick bites seemed to be the risk factors.

Objective: To investigate the relationship between the levels of serum leptin and oxidative stress in patients with hyperglycemia crisis., Methods: A total of 96 patients with diabetic ketoacidosis (DKA) and nonketotic hyperglycemia (NKH) were treated on a low-dose insulin protocol using intravenous infusion of insulin with the established rate of 0.1 Uxkg(-1)xh(-1), with the patients on intravenous fluids and receiving nutrition by mouth and vein. The levels of serum leptin, 8-iso-prostaglandin F(2 alpha) (8-iso-PGF(2 alpha)), the activities of superoxide dismutase (SOD), total antioxidant capacity (TAC) and the contents of malondialdehyde (MDA) in 96 patients with hyperglycemia crisis on admission and after insulin therapy with resolution of hyperglycemia and ketoacidosis (72 hours) were measured. Another 35 healthy individuals served as normal control., Results: The activities of SOD, TAC and the levels of leptin before treatment were lower in patients with hyperglycemia crisis than in normal controls, and the levels of MDA and 8-iso-PGF(2 alpha) were more markedly elevated than those in normal controls (all P<0.05). The activities of SOD, TAC and the levels of leptin in patients after treatment were significantly higher than those in patients before treatment, and the levels of MDA and 8-iso-PGF(2 alpha) were significantly lower than those in patients on admission (all P<0.05). There was significant positive correlation between leptin and MDA in patients before treatment (r=0.38, P<0.05), and the level of leptin was negatively correlated with MDA and 8-iso-PGF(2 alpha) in patients after treatment (r(1)=-0.35, r(2)=-0.37, both P<0.05). In stepwise regression analysis, MDA and 8-iso-PGF(2 alpha) showed a significant association with leptin., Conclusion: The levels of leptin are significantly lowered in patients with hyperglycemia crisis. Oxidative stress may participate in determining the leptin level in hyperglycemia crisis.

Objective: To investigate the characteristics of gene structure in facioscapulohumeral muscular dystrophy (FSHD)-related 4q35 subtelomere, to analyze the distribution of 2 alleles (4qA and 4qB) distal to D4Z4 of this locus, and to further elucidate the genotype-phenotype correlation in Chinese Han FSHD patients., Methods: Peripheral blood samples were collected from 52 unrelated families including 62 FSHD-affected and 57 unaffected members. Genomic DNA was extracted from the lymphocytes according to the specific procedure designed to minimize DNA shearing, then digested with EcoRI or HindIII, or double digested with EcoRI and BlnI. The cleaved DNA was separated by pulsed field electrophoresis (PFGE) and Southern blotting with the probes p13E-11, 4qA, and 4qB. The size of FSHD-causing 4qA allele and its distribution was analyzed by "curve fitting". Then the characteristics of translocation and mosaicism, the frequencies of two allelic variants of chromosome 4q and their genotypes were calculated to analyze the genotype-phenotype correlation., Results: It was found that 69 patients carried a short chromosome 4-type allele of 4qA origin with the length 10-38 kb. The mean length of these pathogenic EcoRI/4qA arrays was 20 kb+/-7 kb, without significant difference between the sporadic cases and familial cases (t=1.413, P>0.05). Three different translocation types were observed with a translocation rate of 14.49%. The rate of 4q-->10q translocation was 13.04%, significantly higher than that of 10q-->4q translocation (1.45%, chi2=6.900, P<0.05). Somatic mosaicism was detected in a male sporadic case and a female asymptomatic familial case. In 57 cases with standard configuration distribution, the frequency of 4qA/4qB heterozygote was 61.40%, significantly higher than that of 4qA/4qA homozygote (38.60%, (chi2=5.930, P<0.05). There were not significant differences in the repeat size distributions and assessment of clinical severity between the sporadic and familial cases (t=-0.039, P>0.05; H=0.693, P>0.05)., Conclusion: About 95% of Chinese FSHD patients carry a pathogenic 4-type allele of 4qA origin less than 30 kb long. The frequent translocations between chromosome 4q and 10q may play an essential role for FSHD mechanism. The frequency of 4qA/4qB heterozygote is significantly higher than that of 4qA/4qA homozygote, while the allelic variant genotypes do not contribute to modify FSHD manifestations.

Aim: To investigate the relationship between adipocytokines levels and oxidative stress in obese males., Methods: The levels of 8-iso-prostaglandinF2alpha(8-iso-PGF2alpha), superoxide dismutase (SOD), malondialdehyde (MDA), adiponectin, leptin, restistin, TNF-receptors 1(TNF-R1), Interleukin-1beta(IL-1beta) and Interleukin-6(IL-6) were measured in obese men and normal controls., Results: The levels of 8-iso-PGF(2alpha), MDA, leptin, TNF-R1, IL-1beta and IL-6 was significantly higher than that normal controls (P<0.05, P<0.01). The levels of adiponectin and the activity of SOD decreased significantly in obese men.There was no significant difference in the restistin between obese men and normal controls. There was significantly positive correlation between 8-iso-PGF(2alpha) and body mass index (BMI) (r=0.54, P<0.05) in obese. A significantly negative correlation was found between 8-iso-PGF(2alpha) and adiponectin (r=-0.56, P<0.05) in obese subjects. The levels of leptin was negative correlated with body fat content(%)(r=-0.53, P<0.05) in obese subjects. A significant negative correlation was observed between the levels of adiponectin and LDL(r=-0.54, P<0.05), IL-6 (r=-0.41, P<0.05). In a multiple regression analysis model, the levels of adiponectin and IL-6 were the main determinants of the oxidative stress in obese men., Conclusion: Changed concentration of adipocytokines was found in obese men. There are significantly correlation with between oxidative stress and adipocytokines.

In the present paper, the interaction of bovine hemoglobin (BHb) and realgar nanoparticles has been investigated by ultraviolet-visible (UV-Vis) and fluorescence spectroscopy. The Soret band of oxygen-BHb at 406 nm shifted to 413 nm, and its absorption intensity decreased gradually after adding realgar nanoparticles. The Soret band decreases gradually with the increasing the amount of realgar NPs, suggesting the detachment of some heme chromophores from their matrixes in BHb. The red-shift of characteristic peak leads to be conjecture that the arsenic of realgar combined with the oxygen of BHb. The oxygen-BHb was deoxidated by realgar nanoparticles, and the surface binding induces conformation change of BHb from the high-affinity R state to the low-affinity T state. The fluorescence intensity of BHb is quenched by realgar nanoparticles when its concentration gradually increased. The analysis of Stern-Volmer equation revealed that the mechanism was a static quenching procedure. The order of the magnitude of binding constant k was 10(9), obtained from the calculation of UV-Vis and fluorescence spectra.