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To study the adsorption to pure oil and oil-water mixture, cattail fiber waddings with different fibers, wadding structures, blending ratios were prepared. Oil absorbencies in the pure oils and oil-water mixtures were tested and evaluated. The analysis showed that cattail fiber waddings have better adsorption performance to pure oil and oil-water mixture, which is higher than 20 g/g. The retention rate of pure oil is higher than 90%, while retention rate of oil-water mixture decreases to 68%. With the increase of wadding thickness and cattail ratio, the oil adsorption and retention of composite waddings are heightened. The oil-retention declines with the blending of polypropylene (PP) fiber. The oil adsorption of kapok fiber is better than that of cattail fiber and PP fiber. Therefore, oil adsorption performance is improved through adjusting the fiber ratio and porous structure.

Objective To investigate the effects of miR-195 on the expression of Sirt1 and its downstream oxidative stress and mitochondrial apoptosis-related proteins in the liver of type 1 diabetic C57BL/6 mice. Methods Mice were divided into control group (Control group), diabetes group (DM group), and miR-195 inhibitor group (DM+antago group). The DM group model was replicated according to the conventional method, and miR-195 inhibitor (2.5 mg/kg) was transfected into DM + antago group mice. The DM group and the control group were injected with scrambled shRNA (2.5 mg/kg) only as a control.Observe and record the increase and decrease of body weight and blood glucose of mice every week. Take fresh liver tissue, one part is processed and made by HE staining for histopathological observation; the part is used to determine the MDA content and T-SOD activity in liver tissue.RT-qPCR and Western blot were used to detect the mRNA and protein content of related indicators in the liver tissue of the three groups of mice. Results 1)Compared with the control group, the leaflets of the liver tissue in the DM group showed structural disorders, hepatocytes were significantly edema, and the cytoplasm was loose; compared with the DM group, the hematocyte edema in the DM+antago group was reduced. 2)Compared with the control group, miR-195 mRNA expression, MDA content and AcFoxo1/Foxo1 protein expression in the DM group were significantly increased (P＜0.05), while Sirt1, Foxo1 mRNA and protein expression, and T-SOD activity were significantly reduced (P＜0.05); Compared with the DM group, miR-195 mRNA expression, MDA content and AcFoxo1/Foxo1 protein expression in the DM + antago group decreased significantly (P＜0.05), while Sirt1, Foxo1 mRNA and protein expression, and T-SOD activity were all Significantly increased (P＜0.05). Conclusions The expression of miR-195 is increased in liver oxidative stress in type 1 diabetic rats. Sirt1 seems to be potential target of miR-195.

[Objectives] To develop a better artificial diet for Spodoptera exigua (Hübner) and thereby improve the captive rearing of this pest. [Methods] Six different artificial diets (1, 2, 3, 4, 5 and 6) were fed to S. exigua larvae, and their effects on larval mortality and adult egg production, as well as differences their utilization rate and nutritional indicators, were compared. [Results] Larvae fed formula 3 had the lowest mortality, the highest utilization rate and adult egg production. [Conclusion] Formula 3, which was mainly comprised of rabbit pellets and wheat germ, can improve the cost-effectiveness of rearing S exigua in captivity. [ABSTRACT FROM AUTHOR]

Objective: To evaluate of the effects of mutations in BCP-A1762T/G1764A and PC-G1896A genes on hepatocarcinogenesis. Methods: Computer searches for PubMed, SCI, CNKI, VIP and WanFang Data databases were conducted to collect literature on the role of mutations in the disease process associated with HBV infection from database creation to July 1, 2021. Two researchers independently screened the articles, extracted information and evaluated the quality of the studies. Review Manager software version 5.4 was used for Meta-analysis. Results: A total of 40 articles were included, with a total of 12 423 cases and 3 710 cases of hepatocellular carcinoma . Meta-analysis showed that mutations in BCP-A1762T/G1764A gene were associated with the disease process of HBV infection and promoted hepatocellular carcinogenesis. mutations in BCP/PC gene were significant in the process of HBV infection in BCP-A1762T/G1764A in HCC vs non-HCC [OR=4.05, 95% CI=2.64 -6.22], CH-BC [OR=3.90, 95% CI=2.13-7.17], CHB [OR=2.77, 95% CI=1.78-4.32], LC [OR=1.64, 95% CI=0.95-2.84], which were statistically significant ; in PC-G1896A mutation HCC vs non-HCC [OR =1.49, 95% CI=1.02-2.17], CHBC [OR=1.56, 95% CI=0.89-2.72], CHB [OR=1.80, 95% CI=1.17-2.77] were statistically significant, while the difference was not statistically significant when comparing HCC with LC (P=0.4). The BCP-A1762T/G1764A mutation in the B genotypes/genotyped versus the C genotype [OR=0.36, 95% CI=0.20-0.64], with a statistically significant difference, and no statistically significant difference in the PC-G1896A mutation. BCP-A1762T/G1764A mutation in the C gene in HCC versus non-HCC [OR=3.71, 95% CI=1.82-7.61] and PC-G1896A mutation in HCC vs non-HCC [OR=2.81, 95% CI=1.34-5.91], the differences were statistically significant. Conclusions: Current evidence suggests that mutations in the BCP-A1762T/ G1764A and PC-G1896A genes have a significant effect on the increased risk of hepatocellular carcinoma and are genotype dependent. However, due to the limitation of the number and quality of included studies, these findings need to be validated by more high-quality studies. [ABSTRACT FROM AUTHOR]

Loss-control urea with nanometer mineral loss control agent added during production period could reduce the loss of nitrogen (N) through adsorption. Figuring out the application rate of loss-control urea and the appropriate rate with conventional urea could provide evidences for summer maize one-time fertilization, N fertilizer reduction, and use efficiency improvement. Under different yield level soil conditions, field experiment was arranged to investigate the effects of different N fertilizer managements on summer maize yield, aboveground biomass, nutrient accumulation, photosynthetic characteristic, and N utilization efficiency. Treatments of conventional urea (N at 210 kg hm-2), loss-control urea (full dose with N at 210 kg hm-2, 10% reduction with N at 189 kg hm-2, and 20% reduction with N at 168 kg hm-2), and different combined proportions of loss-control urea and conventional urea (at 7:3, 5:5, and 3:7) were carried out. The results revealed that N fertilizer application could significantly increase the yield of summer maize and the full-dose loss-control urea treatments increased the yield by 22.96%-27.55%, compared with that under conventional urea application at high- and middle-yield soil conditions. The application of loss-control urea at 10% and 20% reduction sustained the summer maize yield at high- and middle-yield soil condition with improved grains per ear, compared with full-dose loss-control urea application. The N fertilizer utilization efficiency under loss-control urea at 20% reduction treatment at high-yield soil condition reached 41.60%. N accumulation was comparable with full-dose loss-control urea application under proportions of loss-control urea and conventional urea at 7:3 application. Meanwhile, this treatment could sustain the yield and straw biomass at high- and middle-yield level soil and significantly increased the yield and straw biomass at low-yield field. In conclusion, one-time application of loss-control urea with N at 210 kg hm-2 could significantly improve the yield of summer maize and N efficiency of the fertilizer utilization. The application of loss-control urea with 20% reduction could sustain the summer maize yield with significantly improved N utilization efficiency at high- and middle-yield soil conditions, which was the suitable N fertilizer application for high- and middle-yield field. Loss-control urea and conventional urea at 7:3 application was suitable for N fertilizer application for low-yield field. [ABSTRACT FROM AUTHOR]

In the total war and general mobilizatio n system of the Second World War, including the control of leisure and entertainment in the colonies, self-control and frugality was required of the populace. However, as the war continued, there were limited openness and even initiatives to provide leisure and entertainment in line with the situation at that time for people's daily life, becoming a means of boosting morale in the rear and enhancing productivity during a long war. Therefore, leisure and entertainment were not purely objects of repression during wartime, but were also used as intermediary tools for national strength and development. This article focuses on the perspectives of "control" and "regulation." It presents the rulers' control over leisure and entertainment in colonial Taiwan from the beginning of the war between Japan and China to the end of the war in 1945, and the social regulation of the provision of entertainment activities for the people under the prolonged war, as well as the Taiwanese people's actual leisure activities, as their autonomy was slowly lost under the control of external forces. This research finds that after the war between Japan and China began in 1937, entertainment regulations were generally tightened, but not consistently so. There was frequent alternation between relaxation and tightening, and even when regulation was relatively strict, there were also elements of boasting. Since leisure and entertainment became an intermediary tool that affected the development of national power during the war, some entertainment was obviously suppressed, and some was fostered as tools for political propaganda, transformed into entertainment activities that conformed to the situation at that time or actively provided by the ruling party, in order to become "robust entertainment" for national development and boosting morale in the rear. The actual impact of control was different between classes. Presented with limited choices, the lower classes generally had to accept entertainment arranged by the rulers. The upper and middle classes were relatively less affected. However, regardless of class status, it can be seen that some Taiwanese people tried to escape the bans or moved into private realms for self-entertainment, turning leisure and entertainment "underground" in order to maintain its free status. [ABSTRACT FROM AUTHOR]

[Objective] The spatial distribution of agricultural productivity in Guangxi and its influencing factors were studied to improve the efficiency of agricultural productivity in Guangxi. [Method] The agricultural productivities of 14 prefecture-level cities of Guangxi were calculated using DEA model during 2004-2013. Then Moran's I and LISA indexes were used to analyse the erespatial distribution of these cities based on productivity, finally their influence factors were studied using Tobit model. [Result] The results showed that the agricultural productivity of Guangxi had decreased gradually since 2007, due to the decline of scale efficiency mainly, only that of Beihai city kept effective value all the time, those of other cities was constant or descending except Wuzhou and Fangchenggang cities. The regional agricultural productivity was extremely unbalanced, which presented descending trend from south to north and from east to west. The agricultural productivity of Guangxi started to have cluster effect in 2012 and 2013 with the changing of Beihai-centered cluster effect in the low-low type into that in high-high type. In addition, the investment on agricultural mechanization, fertilizer application, government investment, support to education had significantly negative impact on agricultural productivity of Guangxi, while the agricultural science and technology had significantly positive impact on it. [Suggestion] Some suggestions were put forward to achieve sound and fast growth of Guangxi agriculture, including strengthen government 's guidance, promoting synchronous coordinated development of economy and agriculture, realizing resource complementation, developing competitive industries, enhancing radiation function of Beibu Gulf Economic Zone, playing to regional advantages, strengthening spatial spillover effect, improving utilization efficiency of agricultural resources, and enhancing farmers' training. [ABSTRACT FROM AUTHOR]

Generalized distributed fountain codes (GDFC) were proposed as a distributed fountain coding scheme for multi-source single-relay networks. The degree distributions of the relay and sources are optimized by a two-step optimization method using linear and nonlinear programs, respectively. However, the optimization method is with high complexity, and the optimization results depend on the selection of the initial degree distribution. To address this problem, a novel simplified optimization method based on nonlinear program is presented. The proposed method only needs to optimize the degree distributions of the sources and only a simplified one-step optimization is used. Thus, the selection of the initial degree distribution of the sources can be avoided, and the performance can be improved. Compared with the two-step optimization method, the proposed one-step optimization method improves the efficiency and reduces the complexity. Simulations show that the proposed method can enhance the robustness and reliability of the optimization. Simulation results also verify that the bit error rate (BER) can be reduced and the decoding performance of GDFC can be improved. [ABSTRACT FROM AUTHOR]

In this paper, based on the research background of recovering uranium from wet-process phosphoric acid(WPA) by amino phosphonate acid chelating resin, the distribution of uranium in single particle amino phosphonate chelating resin was studied. The results show that the optimal laser frequency of LA-ICP-MS is determined to be 6 Hz, the beam spot diameter is 44 μm, and the energy density is 6 J/cm2. The resin with saturated adsorption and the resin with complete elution were selected as the external standard of the adsorption and leaching process, and the homogeneity test was carried out. The relative standard deviation of uranium content in the resin with saturated adsorption and the resin with complete elution are 3.87% and 3.52%(n=9), respectively. Uniformity is good. In situ microzone analysis of uranium in amino phosphonic acid chelating resin was carried out by LA-ICP-MS. The adsorption process of uranium on the resin is gradually completed from the outside to the inside, and the leaching process is gradually completed from the outside to the inside, and the concentration of uranium inside and outside the resin is gradually uniform and unified, and finally reaches adsorption saturation and leaching complete.

The fracture network of short-distance coal seams group in Liujia Coal Mine is well developed. In order to prevent water conduction caused by fracture in roof and coal seams, gas exceeding the limit in the working face that near goaf, and spontaneous combustion, based on fractal theory, Brazilian splitting test and borehole peeping statistical test, the impact of fragmentation scale and evenness of rock mass on fracture fractal characteristics were investigated. The study shows that the fractal dimension D of split fragmentation is closer to 2, the fragmentation scale becomes more even after fracture. It shows that there is a main crack that roughly divides the sample and the fissure-plane is relatively flat. The fractal dimension D of split fragmentation shows greater difference with 2, the fragmentation scale becomes more uneven and the fissure-plane is rougher. The fractal dimension D of split fragmentation and the fractal dimension D of cracks are in accordance with parabolic distribution. The fractal dimension D of split fragmentation is closer to 2, the bigger the fractal dimension D of cracks. This shows that the fragmentation scale gets more even, the less the number of cracks in the rocks, the more smooth the crack ring and the smaller the angle between the crack ring and interface of rock stratum. The fractal dimension D of split fragmentation shows greater difference with 2, the smaller the fractal dimension D of cracks. This shows that the fragmentation scale gets more uneven, the more the number of cracks in the rocks, the more rough the fissure-plane and the more irregular the crack azimuth. Crack distribution has nothing to do with the splitting fragmentation scale size, but is closely related with the homogeneity of fragmentation scale. [ABSTRACT FROM AUTHOR]

Substituted polycyclic aromatic hydrocarbons (SPAHs) are a type of emerging pollutant that widely exist in the environment, which also exhibit carcinogenicity, mutagenicity, and teratogenicity. These pollutants belong to toxic pollutants because of their similar structures to polycyclic aromatic hydrocarbons (PAHs). Their environmental behavior and ecological risk have attracted increasing attention. Based on a literature review, we found a new breakthrough in the source, distribution, behavior, and risk of SPAHs with comparison to traditional pollutants PAHs. This paper reviewed the current research progress on the environmental occurrence and photochemical behavior of SPAHs. Their sources, formation mechanisms, and distribution characteristics in the multimedia environment were highlighted, and the photochemical transformation kinetics, pathways, and affecting factors of SPAHs in water, ice, and other media were discussed. Furthermore, the research prospects about the environmental behavior and risk of SPAHs were proposed.

Objective: To retrospectively analyze clinical effect of 3D mirror printing combining with minimally invasive percutaneous plate osteosynthesis(MIPPO) technology in the treatment of adult clavicle fracture., Methods: The clinical data of 32 adult patients with collarbone AllmanⅠdisplacement of fracture who had undergone the combination of 3D mirror printing with MIPPO technology treatment were collected from October 2019 to October 2020. There were 19 males and 13 females, aged from 19 to 55 years old with an average of(34.12±16.42) years old. According to Allman classification, there were 14 cases of AllmanⅠb and 18 cases of AllmanⅠc. Fracture occurred on the left in 17 cases and on the right in 15 cases. Postoperative complications, operation time, incision length, intraoperative blood loss, the number of fluoroscopies, fracture healing time of all patients were retrospectively analyzed, and the curative effect was evaluated by Constant-Murley shoulder joint score before operation, 1 and 6 months after operation., Results: All the 32 patients were followed up for 6 to 8 months, with an average of (6.67±2.13) months. All the patients did not have postoperative complications such as incision infection and skin numbness in the operating area. The operation time was (35.37±4.28) min. The incision length was (3.78±0.45) cm. Intraoperative blood loss was(37.23±4.76) ml. The number of fluoroscopies was(3.12±0.47) times. Fracture healing time was (8.18±2.58) weeks. Constant-Murley shoulder joint score of the affected side at 1 and 6 months after operation was 81.08±3.92 and 98.03±1.05 respectively, which had a significant difference compared with 54.62±5.25 before operation( P <0.05). According to Constant-Murley shoulder function score at 6 months after operation, 30 cases were excellent and 2 cases were good., Conclusion: 3D mirror printing can effectively assist MIPPO technique in the treatment of adult clavicle AllmanⅠdisplaced fracture. It not only has the advantages of less trauma and fewer complications of the MIPPO technique, but also can further shorten the operation time and improve the operation maneuverability. It is a better method in the treatment of adult clavicle AllmanⅠdisplaced fracture.

Objectives: To examine the serum levels of degraded monosaccharides in children with Henoch-Schönlein purpura (HSP) and to study the clinical significance of degraded monosaccharides in HSP., Methods: A prospective analysis was performed on 132 children who were diagnosed with HSP from September 2019 to January 2022, and 132 healthy children were enrolled as the control group. High-performance liquid chromatography was used to determine the content of degraded monosaccharides in serum in both groups. The receiver operating characteristic (ROC) curve was used to evaluate the efficiency of degraded monosaccharides for the diagnosis of HSP., Results: Compared with the control group, the HSP group had significantly higher serum levels of mannose, glucosamine, aminogalactose, and galactose ( P <0.001). The four degraded monosaccharides had an area under the ROC curve of 0.919, 0.913, 0.832, and 0.932 respectively for the diagnosis of HSP ( P <0.05)., Conclusions: Children with HSP have higher serum levels of mannose, glucosamine, aminogalactose, and galactose than the healthy population. The levels of degraded monosaccharides may have an important value for the diagnosis of HSP.

Prostaglandins are a class of poly-unsaturated fatty acids-derived bioactive lipids with important physiological function by binding to specific receptors. Prostaglandin receptors lack specific antibodies, which greatly impedes the research on our understanding of the signaling of prostaglandins. The aim of this study was to identify nine mouse lines with amino terminal (-NH2, -N) HA-tagged prostaglandin receptors by using the combination of artificial sperm and CRISPR-Cas9 technology. The guide RNA expression plasmid and labeled targeting vector plasmids were transferred into "artificial sperm cells". The "artificial sperm cells" containing labeled proteins were selected and injected into mouse oocytes, and implanted into pseudopregnant mice to obtain labeled mice. The genomic DNA of the prostaglandin receptor tagged mice was extracted, and the genotypes of mice were detected by PCR method. We also isolated mouse peritoneal macrophages to verify the protein expression of HA-labeled prostaglandin receptor by Western blot. Specific DNA bands were amplified in prostaglandin receptor labeled mice, and specific HA protein bands were detected in macrophage proteins, which was not detected in wild type mice. In summary, we successfully constructed 9 mouse lines with HA-tagged prostaglandin receptors, providing a powerful tool for further study of the pathophysiological functions of prostaglandin signaling both in vivo and in vitro.

Purpose: To investigate the current status of oral health cognition behavior and oral health status of children, and to provide countermeasures for the prevention and treatment of oral diseases in children., Methods: A total of 387 primary school students in the urban area of Shanghai from December 2018 to February 2019 were surveyed using Children's Oral Health Questionnaire and child oral health impact profile（COHIP）. SPSS 24.0 software package was used to conduct statistical analysis of the results through descriptive, univariate and multivariate analysis., Results: The cognition of oral health of children aged 6-9 years old in Shanghai urban area was generally good, but their oral health behavior was average. The caries rate of 387 children reached 57.4%, and the oral health status was not good. Correlation analysis and regression analysis showed that children's oral health behavior was positively correlated with oral health cognition（r=0.260，P<0.05）, and negatively correlated with positive and negative effects of oral health status（r=-0.333，-0.181，P<0.05）, while children's oral health cognition had no significant effect on their oral health status（P>0.05）., Conclusions: The better the oral health behavior habits of children, the greater the positive impact on oral health status; the development of oral health education for children requires more attention to the cultivation of oral health behavior patterns.

Purpose: To study the clinical prognosis of laterally luxated primary teeth after 6-month follow-up without treatment., Methods: Patients with laterally luxated primary teeth, visiting Department of Pediatric Dentistry, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from March 2018 to March 2019, were selected for the study. Based on the inclusion and exclusion criteria, totally 45 patients with 57 primary teeth were included. The reposition outcomes and pulp prognosis were reviewed at the end of follow-up. The data were analyzed using SPSS 23.0 software package., Results: During a 6-month follow-up, 92.98% of the luxated teeth showed spontaneous reposition while only 31.58% of the traumatic teeth were back to the original position. Regarding the pulp healing complications, 54.39% of the luxated teeth exhibited no clinical symptoms, and 14.04% of the evaluated teeth displayed pulp canal obliteration, and pulp necrosis happened in 31.58% of the injured teeth. There was significant difference in the reposition outcome between labial-palatal luxation and mesial-distal luxation(P<0.05), but no significant difference was found in pulp prognosis between the two luxation types(P>0.05). Spontaneous reposition and crown discoloration observed in most of the evaluated cases, were the earliest signs after one-month follow-up. Periapical translucent image and root resorption due to periapical inflammation showed within the first three months after injury, pulp canal obliteration appeared after 6 months., Conclusions: In general, most of the laterally luxated teeth left without treatment can reposition spontaneously and show lower incidence of pulp necrosis compared with mature permanent teeth in half a year after injury. The direction of luxation does not affect pulp prognosis but has an influence on teeth reposition procedure.

Functional soft candy of traditional Chinese medicine( TCM) can be used to supplement vitamins,enhance immunity,assist in lowering blood sugar,assist in lowering blood lipid,assist in lowering blood pressure,relax the bowel,reduce weight and fight againstoxidation.It has attracted much attention due to its effective improvement in the bad taste and incompatibility of traditional medicines.TCM functional soft candy and health food made by gel,sweetener and Chinese herbal medicines( which can be used both as food and medicine) approved by the State Food and Drug Administration were selected as the research objects in this paper.We combed and analyzed the research situation of health food dosage forms and effects,the functional factors of TCM functional soft candy,gelatin,sweetener,production process,and quality evaluation,etc.,and then,we discussed their existing problems and development prospects.After reviewing the relevant literature on TCM functional soft candy and health food researches published in recent years,we analyzed and summarized their dosage forms,health effects,gels,sweeteners,production process,quality evaluation and safety.The following problems in the research of TCM functional soft candy were pointed out: for examples,differences in the sensory evaluation of soft candy;whether the combination of several similar gels and sweeteners had an effect on human health; the lack of laws and regulations in health foods and the lack of uniform standards for quality evaluation.In summary,TCM functional soft candy is a new type of health food,which changes the dosage form of health food based on capsules and tablets.This move conforms to the physiological and psychological needs of consumers.Therefore,it is in line with the requirement of &quot; Health China 2030&quot; Planning Outline.

Objective: To study the biomechanical characteristics of InterTan for the treatment of femoral intertrochanteric fracture of Evans-Jensen IV., Methods: Scanning the femur and internal plant of volunteers with spiral CT to obtain DICOM format data. Three-dimensional models of left femur and InterTan were reconstructed by Mimics software. On this basis, a three-dimensional finite element model of internal fixation for Evans-Jensen IV intertrochanteric fracture of femur was established. The stress and microstrain distribution of Von Mses in different models were studied. The biomechanical stability after internal fixation of the Evans Jensen IV femoral intertrochanteric fracture was analyzed., Results: The stress pattern of the femur of InterTan model was the same as that of the normal femur, which was mainly located on the medial side of the proximal femur and the lower third of the femur. However, the stress of femur in InterTan model was lower than that in the same part of normal femur. The peak stress of the femur in the model was 13.92 MPa, located at the end of the inner plant in contact with the femur. The stress peak of the plant in the model was 146.5 MPa at the lower contact point between the tension nail and the main nail., Conclusions: InterTan fixation has obvious biomechanical advantages and is not easy to cause stress fractures in the middle femur in patients with osteopenic Evans-Jensen IV intertrochanteric fractures. In particular, for patients with greater activity in the intertrochanteric fracture of the Evans-Jensen IV femur, InterTan fixation has better stability and provides a theoretical basis for the choice of internal fixation., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© 2019 by the China Journal of Orthopaedics and Traumatology Press.)

Objective: To investigate the application and effect of minimally invasive percutaneous anterior pelvic pubic ramus screw fixation in Tile B fractures., Methods: A retrospective review was conducted on 56 patients with posterior pelvic ring injury combined with fractures of anterior pubic and ischiadic ramus treated between May 2010 and August 2015, including 31 males and 25 females with an average age of 36.8 years old ranging from 35 to 65 years old. Based on the Tile classification, there were 13 cases of Tile B1 type, 28 cases of Tile B2 type and 15 cases of Tile B3 type. Among them, 26 patients were treated with sacroiliac screws combined with external fixation (external fixator group) and the other 30 patients underwent sacroiliac screw fixation combined with anterior screw fixation (pubic ramus screw group). Postoperative complications, postoperative ambulation time, fracture healing, blood loss, Majeed pelvic function score and visual analogue scale(VAS) were compared between two groups., Results: Fifty-four patients were followed up from 3 to 24 months with a mean of 12 months. There were no significant difference in the peri-operative bleeding and operation time between two groups( P >0.05). The postoperative activity time and fracture healing time of pubic ramus screw group were shorter than those of the external fixator group, the differences were statistically significant( P <0.05). The Majeed score, VAS score of pubic ramus screw group were higher than those of the external fixator group, the differences were statistically significant( P <0.05). The incidence of postoperative complications of pubic ramus screw was lower than that of the external fixator group, the difference was statistically significant ( P <0.05)., Conclusions: Percutaneous iliosacral screws fixation combined with the pubic ramus screw is an effective and safty treatment method to the Tile B pelvic fracture. It has advantages of early ambulation, relief of the pain and few complications., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.

In the present study, the eutrophic level of 30 water samples collected from Lake hongze in August 2014 were analyzed, and the abundance of toxic and non-toxic Microcystis sp., together with their spatial distribution, was investigated by quantitative real-time PCR techniques. The results showed that the average concentrations of total nitrogen and total phosphorus were 1.63 and 0.11 mg x L(-1), respectively. The trophic state index ( TSI) ranged from 58.1 to 73.6, and the water quality was in the state of eutrophication based on TSI. Toxic Microcystis was widely distributed in Lake Hongze, and its abundance varied sharply, from 1. 13 x 10(4) to 3.51 x 10(6) copies x mL(-1), and the abundance of total Microcystis ranged from 1.06 x 10(5) to 1.10 x 10(7) copies x m(-1), meanwhile, the proportion of toxic Microcystis in the total Microcystis ranged from 8.5% to 38.5%, with the average value of 23.6%. Correlation analysis indicated that there was a significant positive correlation among total Mirocystis, toxic Microcystis and the toxic proportion (P < 0.01). The abundance of total and toxic Microcystis was significantly positively correlated to chlorophyll a ( Chl-a) concentrations and TSI (P < 0.01), but was negatively correlated to transparency (SD) (P < 0.01). The ratio of toxic Microcystis to total Microcystis was significantly positively correlated to Chl-a, TN, TP and TSI (P < 0.01), but significantly negatively correlated to the ratio of TN to TP and SD (P < 0.01). Therefore, reducing total nitrogen and phosphorus concentrations could not only lower the eutrophication level of Lake Hongze, but also inhibit the competition advantage of the toxic Microcystis over non-toxic Microcystis.

Objective: To compare the physicians' lipid lowering drug prescribing behavior and knowledge on dyslipidemia before and at 8 months after new-issued blood-lipid reports in our hospital., Method: Blood-lipid reports in our hospital is newly modified in that the classification of dyslipidemia and lipid-lowering guideline and target lipid level are listed on the back of lipid report besides the normal lipid value listed immediately after the measured lipid levels. Physicians' lipid lowering drug prescribing behavior and knowledge on dyslipidemia before and at 8 months after new-issued blood-lipid reports were examined in 143 doctors from various departments before and at 8 months after new-issued lipid reports., Results: At 8 months after the new issued lipid reports, doctors' cognition rate about the guideline was significantly increased [83.9% (120/143) vs. 67.1% (96/143), P < 0.001] and the guideline was considered more helpful on daily practice [75.3% (58/77) vs. 55.8% (43/77), P = 0.005] compared to baseline. However, the prescription rate of dyslipidemia therapy did not change significantly (69.2% vs. 63.2%, P = 0.117) at 8 months after the new issued lipid reports., Conclusions: The modification of the blood-lipid reports improved doctors' knowledge on dyslipidemia and on the "Chinese guidelines on prevention and treatment of dyslipidemia in adults". However, the lipid lowering drug prescribing behavior remained unchanged at 8 months after the modification of the lipid reports. Further investigation is warranted to see if the lipid lowering drug prescribing behavior could be changed in the long-term.

Objective: To investigate the usability of laboratory test report from the angle of patients and understand to what degree the patients master the knowledge of lipid-lowering., Methods: A total of 508 outpatients, selected from a Grade III-A general hospital, were queried by a questionnaire, their medical records and test reports were reviewed and their heights and weights were measured. In the study, 431 of them fulfilled the inclusion criteria and their information about lipid lowering treatment and treatment compliance were collected., Results: Of the 508 subjects, 90.2% (458/508) read the report seriously; however, only 47.4% (240/508) took drugs according to the doctor's prescription even if the tests were "normal". Of the 431 lipid-lowering therapy related patients, only 26.4% (112/431) chose right in their cardiovascular risk classification, and less than 37.1% (160/431) agreed that "different people had different lipid lowering target". Of the 381 patients who needed the lipid-lowering treatment, 71.7% (273/381) recognized the need for treatment, but 98.7% (376/381) answered a wrong target for treatment; 60.9% (232/381) recognized that the reference values given in the laboratory test reports should be the target for treatment. Of the 246 patients under the lipid-lowering treatment, 35.4% (87/246)had reached their treatment goals, and only 52.0% (128/246) had a good compliance., Conclusion: Most patients read and trusted the laboratory test reports. However, dyslipidemia patients scarcely knew their lipid lowering treatment goals and their cardiovascular risk levels. The compliance of patients was poor, and the goal attainment was low. The laboratory medicine department should find out a simple and intuitional way to change the current situation.

Objective: To investigate the association between the -1131T/C and 56C/G polymorphism in the APOA5 gene as well as the -482C/T in the APOC3 gene and susceptibility to coronary artery disease (CAD) in a Chinese Han population., Methods: Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and polyacrylamide gel electrophoresis (PAGE) methods, we analyzed the genotypes in 312 CAD patients diagnosed by angiography and 317 healthy controls. The levels of serum lipid profiles were also studied by biochemical methods., Results: The frequency of the APOA5 -1131 C allele in CAD patients was significantly higher than that of the control group (39.9% vs. 33.3%, P = 0.02). Compared with the wild type TT, CC homozygotes had a significantly increased CAD risk (OR = 1.93 and OR = 1.80 using unadjusted and adjusted logistic regression models, respectively). This association still existed after adjustment for the APOC3-482 variant. The APOA5-1131C allele also showed a correlation with increasing plasma TG levels (P < 0.01)., Conclusions: The APOA5-1131T/C polymorphism but not APOC3-482C/T might contribute to an increased risk of CAD among Chinese accompanied by an elevation of serum TG levels; this effect was found to be independent of the APOC3-482C/T variant.

Objective: To study the changes of serum total calcium (Ca) and inorganic phosphate (P) levels during children growth and related factors., Methods: Using a stratified-multi-steps-cluster sampling method, we selected 2,342 healthy children aged 10-18 years from urban and suburban areas of Beijing from September 2001 to December 2001 to test the levels of serum Ca and P. Their meal status was also surveyed to analyse the correlation of the leves of serum Ca and P with sex, age, height, weight, and diet on statistic basis., Results: The Ca levels of Beijing children were (2.39 +/- 0.12) mmol/L, which had a positive correlation with age, height, and weight (P < 0.01). The Ca levels of female children were somewhat higher than those of male [male, (2.38 +/- 0.12) mmol/L; female, (2.39 +/- 0.12) mmol/L; P < 0.05]. The Ca levels of urban children were significantly higher than those of suburban children [urban, (2.40 +/- 0.13) mmol/L; suburban, (2.37 +/- 0.10) mmol/L; P < 0.001]. The P levels of Beijing children were (1.39 +/- 0.18) mmol/L, which had a negative correlation with age, height, and weight (P < 0.01). The P levels of male children were significantly higher than those of female [male, (1.43 +/- 0.18) mmol/L; female, (1.36 +/- 0.17) mmol/L; P < 0.001]. The P levels of urban children were significant higher than those of suburban children [urban, (1.41 +/- 0.19) mmol/L; suburban, (1.38 +/- 0.16) mmol/L; P < 0.001]. The Ca levels of Beijing children had a negative correlation with P levels (r=-0.141, P < 0.01). [Ca] x [P] (mmol/L) of Beijing children were 3.32 +/- 0.44. The value of [Ca] x [P] reached peak by 3.45 +/- 0.46 when Beijing children were of 13-14 years old, and then the value declined with increasing age., Conclusion: The levels of serum Ca and P correlates with sex, age, growth, and diet. The level of serum Ca goes up while P goes down during the children growth.

Objective: To study the relationship between plasma homocysteine (Hcy) level and deep-vein thrombosis (DVT), and analyze the interaction of Hcy, folate and methylenetetrahydrofolate reductase (MTHFR) gene polymorphism in patients with DVT., Methods: Totally 69 patients with DVT and 111 healthy controls were included in our case-control study. We determined the MTHFR C677T genotypes by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), measured the serum folate and vitamin B12 by radioimmunoassay (RIA), and measured the plasma homocysteine level by fluorescence polarization immunoassay (FPIA)., Results: The frequency of the MTHFR C677T TT genotype had no significant difference between DVT group and control group (P > 0.05). The plasma Hcy level was significantly higher in DVT group than in control group (13.03 +/- 8.74 mumol/L vs 10.14 +/- 4.30 mumol/L, P < 0.05). Both serum folate and VitB12 of patients with DVT were not significantly different from those of controls. The odds radios (OR) of hyperhomocysteinemia for DVT was 2.53 (95% CI 1.08-5.92). The interaction of low folate level and TT genotype increased the risk of DVT (OR = 3.12, 95% CI 1.17-8.38)., Conclusion: Hyperhomocysteinemia may be an independent risk factor for DVT in Han nationality, while serum folate level and MTHRF C677T genotype are not. An interaction between serum folate level and MTHFR genotype that affect the Hcy level is an important risk factor for DVT.

Objective: To evaluate an enzymatic method for determining serum beta-hydroxybutyrate (beta-HB) with the National Committee for Clinical Laboratory Standards (NCCLS) projects, and to discuss its clinical values in diabetic ketoacidosis (DKA)., Methods: The precision, accuracy, specificity, linearity and interference of the enzymatic method were analyzed. This method was used to determine serum beta-HB in 60 cases of normals, 50 cases of diabetes, and 34 cases of DKA by autochemistry analyzer., Results: Enzymatic beta-HB assay was precise (within-run CV, day-to-day CV, and total CV < 5%). The linearity studies showed the method was linear up to 4 mmol/L. Recovery rate was 98.5%-104.1%. Hemolysis (Hemoglobin up to 18.2 g/L), icteric samples with total bilirubin up to 224 mumol/L, and lipemia up to triglyceride concentration of 2.28 mmol/L did not interfere with the beta-HB results in this method. Serum beta-HB levels were significantly elevated in DKA patients compared with DM patients and controls (P < 0.01). Positive rate of serum beta-HB in DKA patients was significantly higher than that of urinary ketone (P < 0.05)., Conclusions: Enzymatic method is convenient and reliable, allows full automation, and is rapid enough to be used for both routine and urgent determinations of serum beta-HB. It can be used in diagnosing and monitoring treatment of DKA.

Objective: To identify the relationship between smoking, alcohol intake and hyperlipidemia in fishermen., Methods: 115 fishermen were randomly recruited and divided into case and control groups according to the result of blood lipoprotein. A questionnaire was used to record general information and the history of smoking and alcohol intake. Statistics were gathered to compare the difference of lipoprotein and apolipoprotein level between exposure and control groups and to calculate the OR value of smoking and alcohol intake., Results: The OR of smoking was 3.417 (95% CI: 1.132 - 10.308), with significant dosage-effect relationship between smoking index and hyperlipidemia. The serum low density lipoprotein-cholesterol (LDL-C) and apolipoprotein (apo) B levels in smoking group was higher than that of control group. The OR value of alcohol intake at early age (early than 20) were 3.275 (95% CI: 1.249 - 8.580) and 4.016 (95% CI: 1.475 - 10.952) respectively. The LDL-C, apoB, the serum total cholesterol (TC)/high density lipoprotein-cholesterol (HDL-C) levels in alcohol abuse group were higher than that of control group., Conclusion: Smoking and alcohol abuse were important risk factors of hyperlipidemia, through changing the level of LDL-C and apoB. There was synergistic action between smoking and alcohol abuse in the development of hyperlipidemia.

Objective: To evaluate the clinical efficacy of two kinds homogenous assays for direct determination of high-density lipoprotein cholesterol (HDL-C) based on the principle of polyanion polymer/detergent (PPD method) and polyethylene glycol-modified enzyme (PEGME) method., Methods: The two homogenous methods were compared with the precipitation method (PTA-Mg2+ method), their precision, accuracy, specificity and interference were also analyzed., Results: Both homogenous HDL-C assays were precise, having a within-run CV < 3%, day-to-day CV < 3% and total CV < 4%. The HDL-C values measured by the two homogenous methods correlated well with those by PTA-Mg2+ method (X): Y = 0.9316 X + 0.1063, r = 0.9762 for PPD method (Y); and Y = 0.9106 X + 0.1368, r = 0.9894 for PEGME method (Y). The linearity studies showed the two homogenous methods to be linear up to 4.14 mmol/L. The lowest detectable concentration of the two methods was apparently 0.08 mmol/L. Recoveries of the two methods were 94.1%-106.2%. Hemoglobin did not interfere with the HDL-C results in the two homogenous methods, whereas icteric samples with total bilirubin > 200 mg/L showed discrepancies. Lipemia up to triglyceride concentration of 17.0 mmol/L did not interfere with the two homogenous HDL-C assays., Conclusions: The two new homogenous HDL-C assays meet the requirements for accuracy, precision, ease of handling with massive sample, allow full automation, and are clinically useful.