1. [Selection and identification of salt-tolerant variants of Taraxacum officinale].
- Author
-
Zhang X, Li Y, Chen H, and Shi W
- Subjects
- Culture Techniques methods, Flavones analysis, Plant Leaves genetics, Plant Leaves growth & development, Random Amplified Polymorphic DNA Technique, Salt-Tolerant Plants genetics, Salt-Tolerant Plants growth & development, Seedlings genetics, Seedlings growth & development, Superoxide Dismutase analysis, Drug Tolerance genetics, Genetic Variation genetics, Sodium Chloride pharmacology, Taraxacum genetics, Taraxacum growth & development
- Abstract
In order to obtain salt-tolerant variant plants of Dandelion (Taraxacum officinale Weber), the leaf discs were excised from 20 to 30-day old seedlings to produce callus, then the induced calli were transferred to selection mediums containing 1.5% NaCl. After regenerating and rooting, these salt-tolerant calli finally developed into 12 variant plantlets. Compared with the wild-type, these regenerated plants produced more trichomes on their leaves, and had larger leaves and shorter petioles. Additionally, the dumpy roots and an approximately 2-cm bract in middle parts of the floricanes were clearly observed in these salt-tolerant plants. By RAPD (Random Amplified Polymorphic DNA) and SDS-PAGE analysis, these salt-tolerant plants showed differences from the control at DNA and protein levels. With 1.5% NaCl treatment, the antioxidant enzyme activity, proline content, and flavonoid concentration were higher in these salt-tolerant plants, whereas maloaldehyde concentration was significantly lower. Salt-tolerant lines of T. officinale showed stronger anti-oxidative activity and higher flavonoid contents.
- Published
- 2008