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Objective To explore the effects of lncRNA ANCR regulating miR-497-5p on vascular lumen formation, apoptosis and PI3K/AKT signaling pathway of gastric cancer cells. Methods Gastric cancer cells were collected and divided into gastric cancer cell (GC) group, gastric cancer cell +lncRNA ANCR-NC (AN) group, gastric cancer cell + lncRNA ANCR overexpressed plasmid (AM) group, gastric cancer cell + lncRNA ANCR siRNA (AI) group, gastric cancer cells + miR-497-5p-NC (MN) group, gastric cancer cells + miR-497-5p siRNA (MI) group, gastric cancer cells +miR-497-5p mimics (MM) group, and gastric cancer cells + lncRNA ANCR siRNA+miR-497-5p mimics (IM) group. Vascular lumen formation was observed by lumen formation assay, cell apoptosis was detected by PI staining method, and protein expression of PI3K and AKT was detected by western blotting. The interaction between lncRNA ANCR and miR-497-5p was confirmed by double luciferase reporting assay. Results Compared with AN group and MN group, the apoptosis rate of AM group and MI group was significantly decreased (P＜0.05), the number of vascular lumen formation and the expression of PI3K and AKT protein were significantly increased (P＜0.05). Compared with AM and MI groups, the apoptosis rate of AI and MM groups was significantly increased (P＜0.05), while the number of vascular lumen formation and the expression of PI3K and AKT protein were significantly decreased (P＜0.05). Compared with AI and MM groups, the apoptosis rate of IM group was significantly increased (P＜0.05), the number of vascular lumen formation and the expression of PI3K and AKT protein were significantly decreased (P＜0.05). The results of double luciferase report showed that transfection of lncRNA ANCR could significantly reduce the luciferase activity of miR-497-5p-3′-UTR-WT (P＜0.05), but had no significant effect on mutant genes (P＞0.05). Conclusion Inhibition of lncRNA ANCR can effectively inhibit the formation of vascular lumen, promote cell apoptosis, and inhibit the expression of PI3K/AKT signaling pathway in gastric cancer cells, which may be related to the targeted activation of miR-497-5p.

Objective: To prepare an effective and water-soluble lubricant., Method: Co-sprayed lubricant (L-leucine and polyethylene glycol 6000 co-sprayed according to a certain proportion) and mixed lubricant (the physical mixture of spayed L-leucine and crushed polyethylene glycol 6000) were prepared and polyethylene glycol 6000, L-leucine, magnesium stearate, sodium stearyl fumarate and sodium chloride are crushed and sieved, respectively. Residual force, appearance of solution and disintegration time were considered as response variables of the lubrication effect to evaluate different lubricants. The changes of the co-sprayed lubricant were studied by differential scanning calorimetry, fourier infrared, electronic scanning microscope and X-ray diffraction., Result: The efficacy of co-sprayed lubricant is better than other lubricants. Efficacy is improved by external form change without inner components and crystal changes., Conclusion: Co-sprayed lubricant is a good water soluble tablet lubricant which has good efficacy.