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Your search keyword '"Sun, Xiangming"' showing total 3 results
Start Over Author "Sun, Xiangming" Language chinese
3 results on '"Sun, Xiangming"'

1. Alice实验内径迹系统探测器升级

Author

Zhang, Biao, Zhang, Li-ang, Deng, Wenjing, Liu, Jun, Zhang, Wenjing, Liu, Donghai, Tan, Yalei, Wang, Yaping, Sun, Xiangming, Yin, Zhongbao, Zhou, Daicui, Huang, Guangming, and Xu, Nu

Subjects
Physics::Instrumentation and Detectors, High Energy Physics::Experiment, Detectors and Experimental Techniques
Abstract

A Large Ion Collider Experiment(ALICE) at the Large Hadron Collider(LHC) at CERN will undergo a major upgrade during the 2nd LHC Long Shutdown(LS2) scheduled in 2019~2021 that will allow to study in detail of strong interaction matter Quark-Gluon Plasma(QGP) properties. To collecting more Pb-Pb events during the Run 3 and Run 4, the replacement of the existing Inner Tracking System(ITS) with a completely new ultra-light high resolution detector based on Monolithic Active Pixel Sensors(MAPS) technique is one of the cornerstones within this upgrade program. The new ITS will consist of seven detection layers: 3 inner layers, 2 middle layers and 2 outer layers. The upgraded ITS will be realized using more than twenty-four thousand pixel chips (called ALPIDE) covering a total active surface of about ten square meters. The thickness of ALPIDE is 50 μm, also with a pixel pitch of 27 μm × 29 μm. The main features of the ALPIDE are of low power consumption, high resolution and high speed readout. ITS2 will enable the ALICE detector to have excellent detection efficiency and impact parameter resolution when measuring extremely low transverse momentum particles, and also make the ALICE detector suitable for LHC high luminosity environment. At present, the production and test of the detector module of ITS2 project have been completed, and the integration of 7-layer barrel been completed at CERN clean room, and the on-surface commissioning has been completed in the end of 2020. The installation plan of ITS2 at the ALICE detector has been started in Jan. 2021, and is scheduled to be finished in end of May 2021. This talk will focus on the design and the physics performance of the new ITS, as well as the advanced techniques adopted in detector assembly and integration will be introduced. The status and commissioning of the ITS upgrade project will be reported.

Published
2020

2. [Studies on influence factors of gnsenoside Rg1 and Rb1 absorption in intestines of rats].

Author

Li W, Nan L, Ji Y, Sun X, and Sun Z

Subjects
Animals, Female, Intestines physiology, Models, Animal, Panax chemistry, Rats, Rats, Wistar, Drugs, Chinese Herbal pharmacokinetics, Ginsenosides pharmacokinetics, Intestinal Absorption
Abstract

Objective: To investigate the absorption of gnsenoside Rg1 and Rb1 in Radix Gngseng at different intestine segments of rats and the influence of the drug solution concentration, pH, P-gp inhibitor., Method: The intestine cannulation was performed for in situ recirculation. Gnsenoside Rg1, Rb1 and phenol red concentration in the flux were separately measured by the reversed phase HPLC and UV., Results: When the concentration was raised from 0.075-0.75 g L(-1) and 0.03-0.3 g L(-1), the uptake of ginsenoside Rg1 and Rb1 was separately linearly increased (r >0.999), and no changes of K(a) absorption fraction and t(1/2) are found. The pH of flux has no effect on drug absorption. Ginsenoside Rg1 can be absorbed in the whole intestine and no changes of K(a), absorption fraction and t(1/2) refound and all the parameters of ginsenoside Rb1 at jejunum are higher than that at ileum and duodenum (P <0. 5). Further more, P-gp inhibitor verapamil has obvious effect on the intestinal absorption of ginsenoside Rb1 (P <0.5) while it has no effect on ginsenoside Rg1., Conclusion: The absorption of ginsenoside Rg1 and Rb1 in intestine of rat are first-order kinetics, the absorption mechanism is infered the passive diffusion. Ginsenoside Rg1 has no specific absorption locus in intestine of rat and ginsenoside Rb1 has specific absorption locus of jejunum. Meanwhile, ginsenoside Rb1 is the P-gp substrate, and could increase its fraction of bioavailability by corporation with P-gp inhibitor.

Published
2009

3. [Transient gene expression of soluble VEGFR2: I-IV].

Author

Li J, Yi X, Zhang Y, and Sun X

Subjects
Animals, CHO Cells, Cell Line, Chorionic Villi metabolism, Cloning, Molecular, Cricetinae, Cricetulus, Culture Media, Serum-Free, Genetic Vectors genetics, Green Fluorescent Proteins genetics, Humans, RNA, Messenger biosynthesis, RNA, Messenger genetics, Recombinant Proteins biosynthesis, Recombinant Proteins genetics, Solubility, Vascular Endothelial Growth Factor Receptor-2 biosynthesis, Gene Expression Regulation, Transfection, Vascular Endothelial Growth Factor Receptor-2 genetics
Abstract

The extracelluar domain I-IV of target gene VEGFR2 (Vascular endothelial growth factor receptor 2) was cloned from villus of trimester abortion by RT-PCR, and linked to the expression vectors. Then, the transfection conditions were optimized in serum-free suspension culture HEK293 using GFP (Green fluorescence protein) as the report gene. The results showed that the optimal transfection efficiency and cell number were obtained when the ratio of foreign DNA: PEI = 1:2 (W/W), DNA = 1.5 g /10(6) cells and shaking speed (120 r/min) in serum free medium in the beginning 4 hours of transfection. After optimizing the transfection conditions, the expression vector was successfully constructed for transient gene expression in HEK293, COS-7, and CHO-K1. The result shows that the target protein was only detected in CHO-K1 supernatant. Because of the C-terminal 8-His tag of target protein, target protein was subsequently purified using Ni2+-IDA and 5 mg purified protein was obtained in 1.5 L supernatant of CHOK1.

Published
2008

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