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Different ultrasonic power treatment effects on in vitro digestibility and oxidation activity of Moringa oleifera seeds water soluble protein (MOWP) were explored by in vitro simulated digestion and antioxidant model. The results showed that during the continuous digestion of gastrointestinal tract, the digestibility and hydrolysis degree were increased obviously, and the digestion was decreased firstly with the increase of ultrasonic power. When the ultrasonic power was up to 650 W, the digestibility of gastric hydrolysate and gastrointestinal continuous digestive hydrolysate could reach up to (68.52±1.70)% and (91.48±0.64)%. After simulated stomach digestion and gastrointestinal continuous digestion, protein molecules were decomposed into proteins with smaller molecular mass, and the peak wave length of endogenous fluorescence changes in the two stages, the peak of stomach digestion was redshifted from 351 nm to 353 nm, and the continuous gastrointestinal digestion was redshifted from 353 nm to 355 nm. The ultrasonic power was 60%, the DPPH • clearance capacity of MOWP after stomach digestion and gastrointestinal continuous digestion was the best, which was (78.26±0.85)% and (44.23±0.98%)%. MOWP-100 had the highest scavenging rates of ABTS free radical in stomach digestion and gastrointestinal continuous digestion stage, which were (91.87±0.19)%. Conclusion: Ultrasonic modification can help improve the digestion performance of MOWP. The ultrasonically digested MOWP has excellent antioxidant activity, which provides theoretical significance for the introduction of MOWP into food and digestion in the human body. [ABSTRACT FROM AUTHOR]

Background: The thoracic small biopsy sampling procedure including transbronchial forceps lung biopsy (TBLB) and endobronchial ultrasound transbronchial needle aspiration (EBUS-TBNA) can be accompanied by rapid on-site evaluation (ROSE) of sample material to provide immediate feedback for the proceduralist. The present study aims to investigate the supplemental effect of ROSE smear samples for lung cancer molecular test., Methods: In a retrospective study, 308 patients admitted to our hospital from August 2020 to December 2022 undergoing diagnostic TBLB and EBUS-TBNA with ROSE and subsequently diagnosed as non-small cell lung cancer (NSCLC) were analyzed. The matched formalin-fixed paraffin-embedding (FFPE) tissue section and ROSE smears for tumor cellularity were compared. DNA yields of smears were determined. Real-time polymerase chain reaction (PCR) and next-generation sequencing (NGS) were performed on adequate smear samples., Results: ROSE smear samples were enriched in tumor cells. Among 308 biopsy samples, 78 cases (25.3%) exhibited inadequate FFPE tissue sections, whereas 44 cases (14.3%) yielded adequate smear samples. Somatic mutations detected in the FFPE tissue section samples were also detected in the matching adequate smear sample., Conclusions: ROSE smear samples of the thoracic small biopsies are beneficial supplemental materials for ancillary testing of lung cancer. Combined use of cytology smear samples with traditional FFPE section samples can enhance the detection rate of informative mutations in patients with advanced NSCLC. We recommend that the laboratory could further evaluate the ROSE cell smears of the patient when FFPE tissue sections are inadequate, and that adequate cell smears can be used as a supplemental source for the molecular testing of NSCLC.