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Objective: To analyze the electrophysiological characteristics and the therapeutic efficacy of irrigated-tip catheter radiofrequency ablation(RFA) without radiation for pregnant women with focal atrial tachycardia(AT) originating from the right atrial appendage (RAA). Methods: Data from 55 women with focal AT, who underwent radiofrequency ablation (RFA) in the First Affiliated Hospital of Zhengzhou University from October 2016 to March 2019, were screened. 2 non-pregnant women with right atrial appendage tachycardia (RAAT) and 4 pregnant women with non-RAAT were excluded. The remaining 49 cases were divided into RAAT during pregnancy group ( n =6, including 4 cases of tachycardia-induced cardiomyopathy) and non-pregnant and non-RAAT group (control, n =43). Under the guidance of three-dimensional mapping system, the earliest activation site was identified, RFA with the irrigated catheter without x-ray fluoroscopy was performed in RAAT patients during pregnancy, all patients in control group underwent non-zero-ray ablation. Patients were followed up at 3, 6, 12 months post procedure, and yearly follow up thereafter in outpatient clinic. Electrocardiogram or Holter monitoring was performed during follow up. AT recurrence and surgical complications were recorded during follow up. At 6 months after RFA, echocardiography examination and laboratory examination including N-terminal B-type brain natriuretic peptide measurement were performed in the pregnant patients, delivery results were also recorded in the pregnant patients. The electrophysiological characteristics of RAAT during pregnancy were analyzed, the therapeutic efficacy of RFA was compared between the two groups. Results: This study is a retrospective study. Age ((30.7±6.2)years vs. (57.2±11.7)years), left ventricular ejection fraction ((46.0±12.8)% vs. (60.1±5.9)%), proportions of organic heart disease (0% vs. 58%) were significantly lower in the RAAT patients during pregnancy group than in control group ( P <0.05), while proportions of patients with persistent tachycardia (100% vs. 7%), symptoms of chest distress and palpitation (6/6 vs. 49%) and left ventricular ejection farction≤50% (4/6 vs. 9%) were significantly higher in RAAT group than in control group ( P <0.05), heart rate was similar between the two groups ((163.7±11.1)beats/minutes vs. (153.7±15.2)beats/minutes, P >0.05). The characteristic P-wave morphology was observed in RAAT patients during pregnancy, i.e, P wave was mostly upright (5/6) in inferior-leads (Ⅱ, Ⅲ, aVF) and in lead I and aVL, deep and wide negative P wave was found in V 1 lead (5/6), and gradually became positive from V 2 -V 6 . The mean tachycardia cycle length was (361.7±38.5) ms. Three-dimensional mapping showed that the origin points of the 6 RAAT pregnant patients were all scattered in the local region, the local region was ablated accordingly, 2 patients (2/6) received extensive ablation of local areas. Immediate successful rate was similar between the two groups (6/6 vs. 93%). During follow up ((15.3±4.0) months), no complications were observed after RFA, postoperative recurrence rate was similar (1/6 vs. 12%). Uncomplicated delivery was reported in all 6 pregnant RAAT post ablation. Normal cardiac structure and function was observed in the 4 pregnant patients with tachycardia-induced cardiomyopathy post ablation. Compared to pre-ablation phase, reduced left atrial dimension ((30.3±1.3) mm vs. (36.8±6.7) mm, P >0.05), increased left ventricular ejection fraction ((64.0±2.9)% vs. (39.8±10.7)%), reduced left ventricular end-diastolic dimension ((44.8±4.0) mm vs. (60.0±2.9) mm) and reduced N-terminal B-type natriuretic peptide value ((136.2±47.5) ng/L vs. (3 408.4±901.3) ng/L) were observed at 6 months post ablation ( P <0.05). Conclusion: The electrophysiological characteristics are suggestive for focal AT originating from RAA during pregnancy. Under the guidance of 3-dimension activation mapping, no fluoroscopic RFA with irrigated-tip catheter is a safe and effective strategy for the treatment of focal RAAT during pregnancy.

Objective: To evaluate the efficacy and safety of three-dimensional electroanatomical mapping system for catheter ablation of paroxysmal supraventricular tachycardiain (PSVT) children. Methods: Clinical data from 187 children with paroxysmal supraventricular tachycardia undergoing radiofrequency catheter ablation in our department between January 2012 and April 2016 were analyzed. Among the patients, 91 cases were treated with traditional two-dimensional X-ray radiofrequency ablation, 96 cases were treated with radiofrequency ablation guided by three-dimensional electroanatomical mapping system. Postoperative electrocardiogram and echocardiography follow-up was performed at 1, 3, 6, 12, and 24 months. The success rate, recurrence rate, complication rate, operation time and amount of X-ray exposure were compared between the two groups. Kaplan-Meier survival curve was used to analyze the PSVT-free survival rate of the patients between the 2 groups. Results: The mean follow-up time was (739±92) days. The success rate (95.8%(92/96) vs. 94.5%(86/91), P= 0.912), recurrence rate (5.4%(5/92) vs. 4.7%(4/86), P= 0.807), complication rate (4.2%(4/96) vs. 5.5%(5/91), P= 0.379), operation time ((73±31)min vs. (79±36)min, P= 0.124) were similar between the two groups. However, X-ray exposure time ((8.1±2.9)min vs. (21.3±8.4)min, P= 0.026), amount of X-ray ((23±11)mGy vs. (58±23)mGy, P= 0.013) were significantly lower in the three-dimensional electroanatomical mapping system group than in the traditional two-dimensional X-ray radio frequency ablation group. PSVT-free survival rate was similar between the two groups (χ(2)=0.060, P= 0.807) . Conclusion: Three-dimensional electroanatomical mapping system is safe and effective for radiofrequency ablation of paroxysmal supraventricular tachycardia in children, and can significantly reduce the amount of radiation as compared to the traditional two-dimensional X-ray radiofrequency ablation.

The aim of this paper is to investigate the effect of patchouli alcohol in enhancing Helicobater pylori's action in eradicating macrophages and its mechanism. H. pylori was co-cultured with macrophages at a ratio of MOI=100 in different concentrations of patchouli alcohol. The effect of patchouli alcohol in eradicating macrophages was detected by agar dilution method. The effect of patchouli alcohol on NO and myeloperoxidase (MPO) levels in macrophages were measured by H. pylori by biochemical methods. Patchouli alcohol effect on H. pylori-induced pro-inflammatory gene expression and protein secretion in macrophages were detected by RT-qPCR and ELISA method. The eradication of H. pylori has significantly enhanced, and the destabilization of lysosomes has been reversed. Meanwhile, patchouli alcohol has an effect in inhibiting pro-inflammation and oxidation. The mechanism of patchouli alcohol in eradicating H. pylori and resisting oxidative stress may be associated to the blocking of bacteria escape lysosome combination procedures., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)

An new type of switch “On-Off” fluorescence probe was constructed based on fluorescence carbon dots as a novel strategy to analyze trace histidine(His) which was proposed for the first time. In water solution with pH 7.6, the fluorescence of CDs was quenched with Ru3+ due to the formation of ground state compound through electrostatic attraction, and the system was thus “turned-off”. The fluorescence intensity of CDs was “turned-on” due to the competition between His and Ru towards the surface of CDs. The effect of critical parameters including pH, buffer solutions, reaction temperature and time needed to grow the fluorescence intensity of CDs was studied. Results show thatin water solution with pH 7.6, and when the temperature was between 20~25 ℃, the fluorescence intensity of the released CDs displayed a linear relationship in the range of (6.5~219.3)×10-6 mol·L-1 of captopril. Lower limit of detection for His, at the signal-to-noise ratio of 3/(3δ), was 2.15×10-6 mol·L-1. The methodology was successfully applied for the determination of His in Compound Amino Acid Injections, with the RSD≤2.07%, and the recovery rate was between 95.7%～102.4%. The result of the experiment was satisfactory. On the one hand, the excellent optical character CDs was acted as “On-Off” fluorescence probe, which could be extent the application of CDs, on the other hand, the excellent performance of the proposed fluorescence probe shows that this method possesses the potential for practical application.

In the present study, the near infrared spectrum of freshwater fish was used to detect the freshness on line, and the near infrared spectra on-line acquisition device was built to get the fish spectrum. In the process of spectrum acquisition, experiment samples move at a speed of 0.5 m · s(-1), the near-infrared diffuse reflection spectrum (900-2,500 nm) could be got for the next analyzing, and SVM was used to build on-line detection model. Sample set partitioning based on joint X-Y distances algo- rithm (SPXY) was used to divide sample set, there were 111 samples in calibration set (57 fresh samples and 54 bad samples), and 37 samples in test set (19 fresh samples and 18 bad samples). Seven spectral preprocessing methods were utilized to prepro- cess the spectrum, and the influences of different methods were compared. Model results indicated that first derivative (FD) with autoscale was the best preprocessing method, the model recognition rate of calibration set was 97.96%, and the recognition rate of test set was 95.92%. In order to improve the modeling speed, it is necessary to optimize the spectra variables. Therefore genetic algorithm (GA), successive projection algorithm (SPA) and competitive adaptive reweighed sampling (CARS) were adopted to select characteristic variables respectively. Finally CARS was proved to be the optimal variable selection method, 10 characteristic wavelengths were selected to develop SVM model, recognition rate of calibration set reached 100%, and recognition rate of test set was 93.88%. The research provided technical reference for freshwater fish freshness online detection.

The randomly placed damage parts of potato will affect the detection accuracy, this paper used transmission and reflection hyperspectral imaging technology to acquire potato images of three directions(the damage part facing to the camera, back to the camera, side to the camera), and then processed the comparative study for damage detection. Independent component (IC) analysis was used to analyze the transmission and reflection hyperspectral images and to extract the features, the resulting char acteristics were used for the secondary IC analysis of the reflected images and the variable selection of the transmittance and re flectance spectroscopy. Finally, the potato injury qualitative recognition model was established based on the reflection images, the reflectance spectral and the transmittance spectral; Further optimization was done for high recognition accuracy of model, and secondary variable selection was carried out for the transmission spectrum by the Sub-window Permutation Analysis(SPA) and the optimal model for damage identification of potato randomly placed was established. The results of experiments show that the accuracy of the identification model based on the reflection image and the reflection spectrum is low, wherein the potato bruise based on the reflection images falls into the lowest recognition accuracy of 43. 10% when it is side to the camera; The accuracy of the model for identification based on the transmittance spectroscopy information is the highest, the recognition accuracy with the damage part facing and back to the camera is 100%t, and 99. 53% when it is side to the camera. The accuracy of the optimal model for identification based on the 3 kinds of transmittance spectroscopy information of randomly placed potato is 97. 39%. Then the application of transmission hyperspectral imaging technology could detect potato injury in any orientation, and the research can provide technical support for the online detection of potato quality.

Objective: To explore the effects of manganese poisoning on the proliferation of neural stem cells (NSCs) in mice's hippocampus., Methods: The mice (weight 8 approximately 10 g) were divided into control group(CG) low-dose group(LDG) middle-dose group(MDG) and high-dose group(HDG)by intraperitoneal injection of 0, 5, 20, 50 mg x kg(-1) x d(-1) of manganese chloride dissolved in physiological saline. The ability of learning and memory was detected by Morris Water Maze, and the proliferation of NSCs in subgranular zone (SGZ) in these mice's hippocampus was also detected by immunohistochemistry., Results: 1) Compared with the CG, the ability of learning and memory in all manganism group decreased significantly (P < 0.01) and this phenomenon in HDG was most notable (P < 0.01). Meanwhile, the ability of memory was negatively correlated with the dose of manganese chloride (r(s) = -0.598, P < 0.01), but the difference of swimming speed in every group was of no statistic significance. (2) The numbers of NSCs in proliferation period in SGZ of all manganism groups was much lower than that of CG (P < 0.01) negatively correlated with the dose of manganese chloride (r(s) = -0.666, P < 0.01). (3) The reduction of NSCs had a positive correlation to the depression of learning and memory (r(s) = 0.734, P < 0.01)., Conclusions: Manganismus can affect the ability of learning and memory, which is probably caused by the inhalation of manganese on NSCs in hippocampus.

Under the conditions of pH 5. 0 HAc-NaAc, 1. 6 X 10(-3) mol x L(-1) KI-8. 0 X 10(-5) mol x L(-1) Fe2+-4. 0X 10(-6) mol x L(-1) H2O2-40 microg x mL(-1) acridine red (AR), when there is H2O2, reacts with I- to form I3- . The acridine red reacts with I3- to form AR-I3 ion association molecule. Under the action of the intermolecular and hydrophobic forces, these association molecules automatically aggregate to form (AR-I3 ), association particles that exhibit three resonance scattering peaks at 320, 400 and 595 nm respectively. The H2O2 concentration in the range of 0. 50-16. OX 10(-6) mol x L(-1) is proportional to the resonance scattering at 400 nm. And a new resonance scattering spectral method was described for the determination of H2O2 in water samples with satisfactory results.

A long-term follow-up study of 21 cases with 24 attacks of acute non-Q-wave myocardial infarction (MI) was reported. These cases had been followed up for an average of 21.1 months, the longest being 7 years and the shortest only 8 days in a fatal case. It was shown that in the acute stage the mortality rate of non-Q-wave MI (14.3%) was slightly lower than that of Q-wave MI (18.9%). However, at the end of 2 years the cumulative mortality rate of non-Q-wave MI (33.3%) was higher than that of Q-wave MI (26.2%) and the rate of reinfarction during the follow-up in the former (38%) was much higher than that in the latter (17.3%). Among the 21 patients, 11 died of cardiac or noncardiac causes during the follow-up. Autopsy was obtained in 9 of the 11 cases. Clinicopathological correlation showed that the present diagnostic criteria for non-Q-wave MI with positive findings in clinical manifestation, electrocardiography and serum enzyme is practical and reliable. 3 of the 9 autopsied cases with negative serum enzyme were also proved to have subendocardial infarction pathologically, but in 2 of them the thickness of the lesion was less than one third of that of ventricular wall. It is, thus, concluded that although non-Q-wave MI has better prognosis in the acute stage, yet its outlook in the long run is by no means good. Moreover, negative serum enzyme does not preclude the possibility of non-Q-wave MI.