Currently, the spatial game between production, living and ecological land is constantly escalating. How to maintain regional ecological security and build a life community of mountains, rivers, forests, farmlands, lakes and grasslands has become the key to the transformation, upgrading and integrated development of urban and rural areas. Taking Baodi District of Tianjin as the study area, this paper applied the comprehensive index evaluation, spatial conflict index model and GIS spatial mutual exclusion matrix to conduct the ecological space regulation zoning based on the internal and external constraints of ecological space suitability and spatial conflict. The results showed that: 1) Under the consideration of suitability and conflict, Baodi District could be divided into six types of ecological regulation space, of which the stable ecological area, protective ecological area, control ecological area, restoration ecological area, reserve ecological area, and non-ecological area accounted for 5. 12%, 4. 59%, 0. 20%,2. 20%,36. 59% and 51. 30%, respectively. 2) A high degree of coincidence was found between the stable ecological area with the ecological protection red line. The overall coincidence rate of them was 71. 64%, indicating that the existing ecological protection red line played an obvious role in the protection of ecological security in Baodi District in terms of scale. 3) There were areas with an abrupt change of high and low ecological suitability levels, and areas with a serious overlap between high suitability and high conflict Meanwhile, most of the ecological protection red lines were directly adjacent to the reserve and non-ecological areas. These patterns indicated that it was difficult to carry out ecological protection according to the spatial distribution of ecological land in Baodi District, for there was a lack of necessary resilient space as a buffer. In conclusion, the ecological space regulation zoning can provide new ideas for increasing the resilience of ecological space, breaking through the bottom-line thinking of ecological protection and realizing the harmonious coexistence of production-living-ecological space. [ABSTRACT FROM AUTHOR]