冯焱1,李兆丰1,2,顾正彪1,2,班宵逢1,孔昊存1,李才明1,2 FENG Yan1, LI Zhaofeng1,2, GU Zhengbiao1,2, BAN Xiaofeng1, KONG Haocun1, LI Caiming1
为了明确酶法制备的环糊精-三丁酸甘油酯包合物(ECT)在动物体内的生物效能,为其在动物饲料中的应用提供理论指导,将36只3周龄雄性BALB/c小鼠随机分为6组,分别为对照组、三丁酸甘油酯组、酶法环糊精组、麦芽糊精-三丁酸甘油酯复合物组、硅基三丁酸甘油酯组、ECT组,饲养5周后测定小鼠体质量、血清生化指标、肝脏抗氧化指标、小肠绒毛高度及隐窝深度、结肠长度、结肠组织学损伤评分、结肠杯状细胞数量以及粪便中短链脂肪酸含量,探究ECT对小鼠生长性能、抗氧化功能和肠道健康的影响。结果表明:与对照组相比,ECT组日均增重增加88.37%,料重比降低45.01%,血清中三酰甘油含量降低38.88%,总抗氧化活力提高36.45%,结肠组织学损伤评分降低,结肠组织中杯状细胞数量增加23.44%,粪便中总短链脂肪酸和丁酸含量分别增加64%和196%。综上,ECT能够提高小鼠的生长性能和饲料利用率,提高肝脏抗氧化能力,还能够促进小肠发育,提高肠道中丁酸等短链脂肪酸含量,预防结肠炎症,有益于肠道健康,具备作为营养型饲料添加剂的应用潜力。 In order to clarify the biological effectiveness of enzymatic cyclodextrin synthesis-tributyrin inclusion complex (ECT) in animals and provide theoretical guidance for its application in animal feed, 36 3-week-old BALB/c male mice were randomly divided into 6 groups: control group, tributyrin group, enzymatic cyclodextrin group, maltodextrin-tributyrin complex group, silica-tributyrin group, and ECT group. After 5 weeks of feeding, the body weight, serum biochemical indexes, liver antioxidant indexes, villus height and crypt depth of small intestine, colon length, colonic histological injury score, colonic goblet cell number and short-chain fatty acid contents in faeces were measured to explore the effects of ECT on the growth performance, antioxidant function and intestinal health of mice. The results showed that compared with the control group, the ECT group increased the average daily weight gain by 88.37%, reduced the feed/gain by 45.01%, reduced the serum triglyceride content by 38.88%, increased the total antioxidant activity by 3645%, reduced the colonic histological injury score, and increased the number of goblet cells in colon tissue by 23.44%. Total short-chain fatty acids and butyric acid contents in faeces increased by 64% and 196%, respectively. In conclusion, ECT can improve the growth performance and feed utilization rate of mice, improve the antioxidant capacity of the liver, promote the development of the small intestine, increase the content of short-chain fatty acids such as butyric acid in the intestine, prevent colon inflammation, and benefit intestinal health. ECT has the application potential as a nutritional feed additive.