Search

Objective　 To investigate the effect of interfering with the expression of VEGF-C on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cell transplanted tumor and related signaling pathways. Methods　The mice with transplanted tumors of nasopharyngeal carcinoma CNE-2 were randomly divided into tumor body group，siRNA-NC group， siRNA-VEGF-C group; irradiation group；siRNA-NC+ irradiation group; siRNA-VEGF-C+irradiation group. Tumor growth was observed，and the protein expression of VEGF-C，ATM and P65 were detected by immunohistochemistry. Results　Interference of VEGF-C expression in nasopharyngeal carcinoma CNE-2 cell transplanted tumors showed significantly slower tumor growth after irradiation. Immunohistochemical test results showed that after tumor transplantation of mice interfered with VEGF-C，VEGFC and ATM were significantly decreased （ P < 0.05）， while P65 was increased （ P < 0.05） . After combined irradiation，VEGF-C and ATM were significantly decreased （P < 0.05）. Conclusion　In mouse nasopharyngeal carcinoma CNE-2 cell transplantation tumors，interfering with VEGF-C gene affects downstream ATM by activating NF-kB signaling pathway，thereby increasing the sensitivity of radiotherapy. [ABSTRACT FROM AUTHOR]

Objective: To study the expression of regenerating islet-derived family member 4 (RegⅣ) in retinoblastoma and its correlation with angiogenesis. Methods: 16 cases of patients with retinoblastoma who underwent operation in our hospital from June 2012 to June 2014 were selected as the research object.The positive expression rates of RegⅣ, VEGF-A and VEGF-C between retinoblastoma tissues and normal tissues were compared, the correlation of clinicopathological features with the expressions of RegⅣ, VEGF-A and VEGF-C were analyzed, and theh microvessel density (MVD) between RegⅣ positive expression tumor tissue and normal tissue were compared. Results: Compared with the normal tissues, the Reg Ⅳ, VEGF-A and VEGF-C expressions were higher in the retinoblastoma tissues (P＜0.05). The Reg Ⅳ,VEGF-A, VEGF-C expressions were significantly correlated with the clinical stage,pathological type,optic nerve invasion retinoblastoma (P＜0.05), but had nothing to do with gender, site of onset (P＞0.05). Pearson correlation analysis showed the expression of Reg Ⅳ was positively correlated with VEGF-A(r=0.696,P＜0.05); the expression of Reg Ⅳ was positively correlated with VEGF-C(r=0.728,P＜0.05). The MVD value of Reg Ⅳ positive expression was (59.67± 1.43), which was significantly higher than that of the normal retinal tissues (30.53± 2.31) (P＜0.05); the MVD value of Reg Ⅳ negative expression tissues was (38.04± 2.86), which had no difference compared with the normal retinal tissues (P＞0.05). Conclusions: The positive expression rates of Reg Ⅳ was highly expressed in the retinoblastoma tissue and correlated with the invasion and migration, it could accelerate the development of the retina through promoting the angiogenesis mediated by VEGF-A and VEGF-C. [ABSTRACT FROM AUTHOR]

Objective: To investigate the mechanism of SDF-1 /CXCR4 and VEGF-C in laryngeal cancer lymph node metastasis.Methods: 90 cases of laryngeal cancer patients in our hospital from August 2012 to August 2015 were randomly selected, these patients were regarded as the study group, and the other 20 cases of patients with the corresponding normal tissue were selected as the control group, the CXCR4, VEGF-C and SDF-1 m RNA expression were detected by RT-PCR method, and the VEGF-C CXCR4 protein and SDF-1 protein were detected by immunohistochemistry SP method, and then the relationship between the SDF-1/CXCR4 expression and VEGF-C expression and clinicopathological features in laryngeal lymph node tissue, CXCR4 and VEGF-C in laryngeal carcinoma were analyzed.Results: The CXCR4, VEGF-C, SDF-1m RNA, protein expression of carcinoma were significantly higher than normal tissues(P<0.05); the CXCR4, VEGF-C, SDF-1m RNA, protein expression positive rates of III + IV patients were significantly higher than Ⅰ+ Ⅱpatients(P<0.05); the CXCR4, VEGF-Cm RNA, protein expression of poorly differentiated patients were significantly higher than high +differentiation patients(P<0.05); the CXCR4, VEGF-C, SDF-1m RNA, protein expressions of lymph node metastasis patients were significantly higher than patients without lymph node metastasis(P<0.05); the CXCR4 protein and VEGF-Cm RNA positive expressions of laryngeal carcinoma showed significant positive correlations(P<0.05), negative also showed significant positive correlations(P<0.05). Conclusion: SDF-1/CXCR4 and VEGF-C in laryngeal cancer lymph node metastasis have higher expression, they may promote laryngeal lymph node metastases together. [ABSTRACT FROM AUTHOR]

Objective: To analyze the changes in tumor lymphatic vessel density (LVD) in patients with lung adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA), and invasive adenocarcinoma (IA) and explore the regulatory factors of LVD., Methods: Complete clinicopathological data were collected form a total of 301 patients with lung adenocarcinoma, including 28 (9.3%) with AIS, 86 (28.6%) with MIA, and 187 (62.1%) with IA. The LVD of all the adenocarcinomas were calculated after D2-40 immunohistochemical staining, and MT1-MMP and VEGF-C expression levels were also evaluated. The differences in LVD among the groups and the correlations of tumor LVD with the expressions of MT1-MMP and VEGF-C and the clinicopathological factors were analyzed., Results: The LVD differed significantly among AIS, MIA, and IA groups ( P = 0.000). The LVDs was significantly correlated with the level of VEGF-C protein expression ( r =0.917, P =0.009), tumor size ( r = 0.686, P =0.017), lymph node metastasis ( r =0.739, P =0.000), and clinical stage ( r =0.874, P =0.012) of the patients., Conclusions: Tumor lymphangiogenesis plays an important role in lung adenocarcinoma progression, and VEGF-C may promote this process.