1. 血管内皮生长因子-C 对鼻咽癌CNE-2 细胞小鼠移植瘤的 辐射敏感性的影响.
- Author
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吴锡芳, 汪 勇, 彭丽莎, 罗 洁, and 王 枫
- Abstract
Objective To investigate the effect of interfering with the expression of VEGF-C on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cell transplanted tumor and related signaling pathways. Methods The mice with transplanted tumors of nasopharyngeal carcinoma CNE-2 were randomly divided into tumor body group,siRNA-NC group, siRNA-VEGF-C group; irradiation group;siRNA-NC+ irradiation group; siRNA-VEGF-C+irradiation group. Tumor growth was observed,and the protein expression of VEGF-C,ATM and P65 were detected by immunohistochemistry. Results Interference of VEGF-C expression in nasopharyngeal carcinoma CNE-2 cell transplanted tumors showed significantly slower tumor growth after irradiation. Immunohistochemical test results showed that after tumor transplantation of mice interfered with VEGF-C,VEGFC and ATM were significantly decreased ( P < 0.05), while P65 was increased ( P < 0.05) . After combined irradiation,VEGF-C and ATM were significantly decreased (P < 0.05). Conclusion In mouse nasopharyngeal carcinoma CNE-2 cell transplantation tumors,interfering with VEGF-C gene affects downstream ATM by activating NF-kB signaling pathway,thereby increasing the sensitivity of radiotherapy. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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