1. [Effect of a novel fibrinolytic enzyme FA-I on thrombosis and thrombolysis].
- Author
-
Wang GX, Liang CJ, Yao JY, Yuan JL, and Wang JF
- Subjects
- Animals, Bacterial Proteins chemistry, Bacterial Proteins pharmacology, Enzyme Stability, Fermentation, Fibrinolysin chemistry, Fibrinolysis, Fibrinolytic Agents therapeutic use, Rabbits, Rats, Bacteria enzymology, Bacterial Proteins isolation & purification, Carotid Artery Thrombosis drug therapy, Fibrinolytic Agents isolation & purification, Venous Thrombosis drug therapy
- Abstract
Objective: To study the effect of FA-I, a novel fibrinolytic enzyme isolated from the metabolite of Arthrobacter sp. DR-536, on thrombosis and thrombolysis in vivo., Methods: The anticoagulated blood model of mise were administered with FA-I orally. The venous thrombogenesis inhibition model of rats were administered with FA-I by intestinal route. The carotid thrombosis model of rabbits were given FA-I intravenously. The clotting time (CT), prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), Euglobulin lysis time (ELT), fibrinogen (FIG) and hemorheology condition were analyzed., Results: FA-I prolonged the CT, PT, APTT and TT, and decreased ELT and FIG significantly (P < 0.05 or P < 0.01). FA-I also improved the hemorheology conditions in the rabbits., Conclusion: Both intravenous injection and oral administration of FA-I are effective in thrombosis and thrombolysis. FA-I could become a pragmatic venoclysis thrombolytic drug or a peroral thrombolytic drug.
- Published
- 2009