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Objective To investigate the role and molecular mechanism of connexin 43 (Cx43) in rats with myocardial injury induced by common bile duct ligation (CBDL). Methods A total of 18 Sprague-Dawley rats were randomly divided into sham-operation group, CBDL group, and Cx43 inhibitor group, with 6 rats in each group. The rats in the sham-operation group were given isolation of the common bile duct, those in the CBDL group were given isolation, ligation, and cutting of the common bile duct, and those in the Cx43 inhibitor group were given a single injection of 25 mg/kg Gap 26 via the caudal vein at 24 h before surgery, followed by the isolation, ligation, and cutting of the common bile duct. The rats in all three groups were reared to day 14 after surgery, and echocardiography was used to evaluate ejection fraction (EF), fractional shortening (FS), and heart rate (HR); the three groups were measured in terms of serum total bilirubin (TBil), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) activity, and malondialdehyde (MDA); HE staining and Masson staining were performed for heart tissues samples collected from the three groups; Western blotting was used to measure the levels of Cx43, Wnt 3α, and β-catenin in heart tissue. Results Echocardiography showed that the CBDL group and the Cx43 inhibitor group had significantly lower cardiac EF, FS, and HR than the sham-operation group (F=22.95-43.55,t=5.10-9.32,P

Background The occurrence of postoperative complications in lumbar degenerative disease (LDD) is closely related to malnutrition, and currently there is a lack of nutritional evaluation methods that can effectively, objectively, and comprehensively assess the nutritional status of LDD patients and predict the occurrence of postoperative complications. Objective To compare the predictive value of four nutritional evaluation methods of Prognostic Nutritional Index (PNI), Controlling Nutritional Status (CONUT), Naples Prognostic Score (NPS) and High-sensitivity Modified Glasgow Prognostic Score (HS-mGPS) for postoperative complications in patients with LDD, so as to formulate a precise nutritional intervention plan and effectively prevent complications. Methods A total of 201 patients diagnosed with LDD and underwent open surgery in Spine Surgery Department of Xiangya Hospital of Central South University from December 2021 to July 2022 were selected as the study subjects, four nutritional evaluation methods of PNI, CONUT, NPS and HS-mGPS were applied to assess the preoperative nutritional status of the patients, respectively, and occurrence of postoperative complications from postoperative period to hospital discharge were observed. The patients were divided into the complication group and non-complication group according to the occurrence of postoperative complications to analyze and compare the difference in clinical characteristics between the two groups; the optimal cut-off values of different nutritional evaluation methods were calculated by plotting the receiver operating characteristic (ROC) curve, and the prediction efficiency of different evaluation methods was compared, the area under curve (AUC) was compared by using MedCalc software. Multivariate Logistic regression model was used to analyze the correlation between different nutritional evaluation methods and postoperative complications. Results Postoperative complications occurred in 60 patients with an incidence rate of 29.8%. The comparison of clinical characteristics data showed statistically significant differences in age, gender, intraoperative bleeding, surgical segment, PNI, CONUT, and NPS scores between the complication group and non-complication group (P

To obtain a full understanding the quality and microbial characteristics of soybean paste made from a mixture of soybean and broomcorn millet flour, its physicochemical properties (amino nitrogen and nitrite), and total phenols (TP), γ-aminobutyric acid (GABA), free amino acids (FAAs), volatile compounds, and microbial community composition were investigated. The results showed that the amino nitrogen content increased to 0.71%, and the nitrite content decreased to within the standard range (1.37 mg/kg). The contents of TP, key FAAs and volatile compounds increased significantly during the fermentation process. The core microbial communities included Enterobacter, Pseudomonas, Stenotrophomonas, Aspergillus, and Alternaria. The results of correlation analysis confirmed that bacteria (Bacillus, Knoellia, and Blastococcus) and fungi (Epicoccum and Saccharomyces) played a significant role in the bioactivity changes and flavor generation in soybean paste. This study will be of great significance for understanding the quality and flavor of novel soybean paste made with cereal flour as an additive.

Objective·To investigate the occurrence of new onset anxiety symptoms in children and adolescents during the COVID-19 epidemic, and analyze the influencing factors.Methods·Based on Chengdu Positive Child Development (CPCD) cohort, a total of 5 566 children and adolescents from five primary and secondary schools in Chengdu were enrolled. Two longitudinal study cohorts of new anxiety symptoms in children and adolescents were established, and two rounds of survey were conducted. The first round of survey (baseline survey) was conducted from December 2019 to January 2020, and the general demographic characteristics of the cohort members were collected through the Student Questionnaire for the Study on Promoting Positive Growth of Children and Adolescents. The second round of survey (follow-up survey) was conducted from February to July 2020 to collect additional information on the cohorts' infection history of COVID-19, and whether their eating, learning, and social and recreational activities were affected by the COVID-19 epidemic. The Screen for Child Anxiety Related Emotional Disorders (SCARED) was used to evaluate the new onset anxiety symptoms of all subjects. The multivariate Logistic regression model was used to analyze the influencing factors of new onset anxiety symptoms in children and adolescents.Results·The results of SCARED assessment showed that the incidence of new onset anxiety symptoms among children and adolescents in Chengdu during the COVID-19 epidemic was 13.47%. In the longitudinal study cohort of new onset anxiety in children,the incidence was 11.91%, and in the longitudinal study cohort of new onset anxiety in adolescents, the incidence was 14.25%. The results of chi square test showed that there were statistically significant differences in the incidence of new onset anxiety symptoms among children in terms of age, whether they or their family members were infected with COVID-19, and whether their eating, learning and social activities were affected (all P

Background There is no safe and effective antidote for cardiotoxicity induced by bupivacaine, an extensively used anaesthetic. Our previous research shows that acupoint treatment may partially improve bupivacaine-induced cardiotoxicity, but the mechanism of action needs to be further studied. Objective To observe the effect of acupoint injection of puerarin on mitochondrial energy metabolism in myocardia in bupivacaine-poisoned rats, and to explore its mechanism of action. Methods This experiment was conducted from January to December 2019. Forty 6-month-old male SPF Wistar rats were selected and equally randomized into a blank group (group C) , a model group (group B) , a treatment group (group T) , and a prevention group (group P) . Interventions were given to the groups as follows: Group P received: 0.1 ml puerarin injection injected into bilateral Neiguan acupoints. Then 5 minutes later, bupivacaine-poisoned model was established for three groups (except for group C) using intravenous infusion of 0.5% bupivacaine (10 mg/kg) via femoral vein within 2 minutes. Group C received 0.1 ml of 0.9% sodium chloride solution injected into bilateral Neiguan acpoints. : Immediately after successful modeling, 0.1 ml of 0.9% sodium chloride solution was injected into bilateral Neiguan acupoints for group B, and: 0.1 ml puerarin injection was injected into bilateral Neiguan points for C. The rats in group C were sacrificed at the 8th minute of intravenous infusion of 0.9% sodium chloride solution, and those in groups B, T, and P were sacrificed at the 8th minute of intravenous infusion of bupivacaine, then the left ventricular myocardium of all rats were taken out and cardiomyocyte mitochondria were isolated, the structure of which was observed under the transmission electron microscope. The semi-quantitative analysis of mitochondrial structure was evaluated by Flameng classification system. The activity of mitochondrial adenosine triphosphate synthase (ATPase) was measured by ELISA. The content of adenosine triphosphate (ATP) was measured by colorimetric assay. The mitochondrial membrane potential (MMP) was detected by JC-1 fluorescence staining and colorimetry. The protein levels of myocardial mitochondrial peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) , nuclear respiratory factor 1 (NRF1) , and mitochondrial transcription factor A (mtTFA) were measured by Western blotting. Results Group C had lower Flameng score than did other three groups (P

The watershed carbon cycle is an important part of the global carbon cycle, playing an important role in seeking global carbon sinks that have been missed. Dissolved inorganic carbon (DIC) is one of the important indicators for studying the carbon cycle of water bodies in the basin. As an important component of terrestrial ecosystems, karst water bodies containing high DIC concentrations play an important role in the global carbon cycle. The Lujiang river basin, a typical development area of karst graben basin, is an applicable place for us to study the carbon migration and transformation of water bodies. In order to study the hydrochemical types and sources of DIC in the rivers of the Lujiang river basin, sampling data of hydrochemistry and carbon isotope from 14 surface water points, 8 underground river points, and 10 spring points during the rainy season were analyzed. It is believed that exploring the proportional contribution of DIC sources in different water bodies may help to understand the sources and cycles of groundwater DIC under the influence of human activities, providing scientific references for water resource management. Firstly, this study determines the hydrochemical types of different water bodies through the Piper trilinear map and Schukalev classification. The results show that regardless of the type of water body in the study area, the hydrochemical type in the drainage basin is mainly HCO3-Ca, belonging to the "type of carbonate rock weathering". Due to the long interaction time between water and rock, the rock weathering in groundwater is more obvious than in surface water. Secondly, the analyses of ion sources in water bodies by Gibbs plot indicate the consistency in the hydrochemical composition, changes, and origin mechanisms between groundwater and surface water. The controlled mechanism belongs to the "leaching type of rock weathering". Thirdly, due to the widespread distribution of carbonate rocks in the study area, an analysis has been conducted on carbonate rock dissolution in order to distinguish the relative contribution of limestone and dolomite dissolution to the chemical ion compositions of water bodies in the watershed. It is found that there are additional sources of Mg2+ and Ca2+ in the surface water of the Lujiang river basin, while other acids participate in the chemical weathering reaction in the water bodies of the underground river points and spring points. Furthermore, it is determined that SO42- and NO3- are involved in the rock weathering in the basin, which has caused a large disturbance to the hydrochemical characteristics of water bodies in the basin. The Lujiang river basin is mixed with carbonate and silicate rocks. Therefore, the main sources of HCO3- in water are classified into three types: carbonate rocks weathered and dissolved by carbonic acid, carbonate rocks weathered by sulfuric acid/nitric acid, and silicate rocks weathered by carbonic acid. Based on the quantitative analysis by ion ratio method, carbonate rocks weathered by carbonic acid contribute average 68.8% to HCO3-, carbonate rocks weathered by sulfuric acid/nitric acid 27.2%, and silicate rock weathered by carbonic acid 3.9%. During the weathering process of water bodies in the Lujiang river basin, carbonate rocks are mainly weathered by carbonic acid. Finally, the verification by carbon isotope method has been compared with the measured values to show that the theoretical average values of underground river water and spring water δ13CDIC_Th are relatively close, while the values of surface water are positive too, indicating that the source of DIC in surface water is not only controlled by rock weathering, but also by biogeochemical processes within river water bodies. In comparison, the areas of surface rivers are more significantly affected by human activities with severe urbanization and agricultural activities. [ABSTRACT FROM AUTHOR]

In order to investigate the effects of combined contamination of the typical pollutants, such as pesticides and heavy metals, on the ecological environment, the ammonifying bacteria, a common strain in nature, was selected as an experimental study object. The experiment was conducted with cultivated soil as the bacterial source, a strain of ammoniated bacteria (Z1) was isolated and screened, which was identified as Pseudomonas. It was showed that the ammonification rate reached 0.792 mg/(L•h) at 12 h, and the amount of ammonia nitrogen produced was up to 22.500 mg/L at 48 h. In the case of glyphosate (GLY) and heavy metal cadmium (Cd) being used as target pollutants, the binary mixture system (GLY plus Cd) was thereby designed by the direct equipartition ray method; by adopting the time-dependent toxicity microplate analysis method, the efffects of GLY, Cd and the binary mixture system on Z1 were respectively determined about the concentration-effect data at different exposure time; and toxicity interaction type and intensity of the components of GLY-Cd mixture were analyzed by fitting zeroing method. As a result of the experimental study, the toxicity of a single pollutant to Z1 gradually increased as its concentration went up, with the characteristics of concentration and time dependent toxicity. The toxicity of Cd to Z1 was always greater than GLY to Z1; the five rays (from R1 to R5) of the GLY-Cd mixture system were characterized with dependent toxicity in terms of concentration, time and component-ratio, and the toxicity interaction mainly appeared to be antagonism, and R3 had the highest dCA value (65.4%) at 8 h. In summary, the combined pollution of GLY and Cd reduced their toxicity and bio-availability in soil. [ABSTRACT FROM AUTHOR]

When transporting natural gas, long-distance pipelines will inevitably pass through areas with frequent geological disasters, and landslides are the most threatening to pipelines. Therefore, it is very important to study the safety assessment of pipelines under the action of landslides. In view of the possible safety problems of X80 pipeline crossing the landslide, the definition of the limit landslide displacement of the pipeline is proposed as an external index to evaluate the safety of the pipeline. According to the working condition of a landslide on the Zhongwei Guiyang line, the pipe soil model under the landslide is established by using ABAQUS software, and the mechanical behavior of the pipeline and the change of the limit landslide displacement under different landslide cracks and landslide widths are numerically simulated. The results show that the pipeline stress increases with the increase of displacement load under different crack positions and landslide widths; The farther the landslide crack location is from the pipeline, the greater the limit landslide displacement that the pipeline can bear. When the crack location is 9 m, the limit landslide displacement that the pipeline can bear reaches 4.03 m; Under the working condition of this paper, the landslide width of 35 m is the dividing point of pipeline stress change. At this time, the limit landslide displacement that the pipeline can withstand is the smallest. [ABSTRACT FROM AUTHOR]

Five different drying temperatures, that is 40, 45, 50, 55, and 60, and natural drying (temperature range 30-35 ) were set for the drying test to explore a suitable temperature condition for drying wolfberry (Lycium barbarum). The physicochemical indicators were analyzed and tested in terms of the effects of temperature on drying duration, rehydration ratio, color, browning degree, and bioactive substances such as Lycium barbarum polysaccharide (LBP), flavonoids, and carotenoids.An optimal drying temperature was selected by determining the weights with the coefficient of variation method. The results showed that the nutritional composition of Lycium barbarum products dried at the five different temperatures changed significantly. The temperature had relatively great influences on five indicators, namely drying duration (weight: 0.317 0), browning degree (weight: 0.224 7), rehydration ratio (weight: 0.141 4), flavonoids (weight: 0.100 1), and LBP (weight: 0.065 2), among which drying duration was most affected. The results of the overall evaluation of drying temperatures were as follows: drying under 45° (overall score: 0.430 5) > drying at 40° (overall score: 0.402 6) > natural drying (overall score: 0.140 7) > drying at 50° (overall score: 0.011 2) > drying at 55° (overall score: -0.317 6) > drying at 60° (overall score: -0.668 2). The highest score was achieved at 45°, and the quality of wolfberry was the best at this temperature. Therefore, 45° was selected as the optimal drying condition. [ABSTRACT FROM AUTHOR]

To investigate the filtration effect of tobacco rod on smoke components in mainstream smoke, a method for analyzing the filtration by the residual tobacco section to smoke components was developed. A cigarette without its filter was lit without puffing and smouldered to the marked position of 15 mm to form a stable combustion cone, and following a puff by a linear smoking machine the residual tobacco section was cut into multiple subsections. The contents of 32 aerosol components filtered by each subsection were determined by GC-MS after extracting with dichloromethane. Six major smoke components, including nicotine, furfural, furfuryl alcohol, phenol, 4-methylphenol and indole, were selected as markers to reflect the filtration rules of tobacco section. The results showed that: 1) The filtration efficiencies of tobacco section to the target smoke components were between 12.0% and 73.6%. 2) The first subsection of 25 mm length adjacent to the combustion cone had stronger filtration to smoke components, which might be attributed to the condensation and adsorption of components in vapor phase. In comparison, the downstream subsection of tobacco rod had a weaker filtration effect, which mainly filtrated aerosol particles. This method is suitable to study the filtration rules of tobacco section to the 32 mainstream smoke components. [ABSTRACT FROM AUTHOR]

In order to investigate the releases of aldehydes and ketones in mainstream smoke of cigarettes of different sizes, a gas chromatography-tandem mass spectrometry (GC-MS/MS) method was developed for simultaneous determining 95 aldehyde and ketone components in mainstream cigarette smoke. The samples of slim, medium and king-sized cigarettes were prepared with the same tobacco blend. Eight irritants with lower molecular weights and 95 aroma components of aldehydes and ketones were measured. One-way analysis of variance (ANOVA) was used to screen the different compounds. The results showed that: 1) The calibration curves of all the aroma components presented good linearity (R²>0.99). The intra- and inter-day relative standard deviations (RSDs) were in the ranges of 1.4%-11.1% and 0.7%-24.3% respectively, wherein the RSDs of 70 compounds were less than 10%. The recoveries of 92 compounds ranged from 70% to 120% at three spiked levels. 2) A total of 68 different compounds were measured. The releases per unit tar for seven irritant components (except formaldehyde) with lower molecular weights from the king-sized cigarettes were higher than those from the medium and slim cigarettes. 3) For aldehydes and ketones with boiling points ≤133 °C, such as 2-hexanone, acetaldehyde, and 2,3-pentandione, their releases were higher from the king-sized cigarettes than those from the medium and slim cigarettes. For aldehydes and ketones (except 3-methylacetophenone and cinnamaldehyde) with boiling points ≥215 °C, such as 2-hydroxy-3-methyl-2-cyclopentene-1-one, vanillin and 5-hydroxymethylfurfural, their releases were significantly lower from the king-sized cigarettes than those from the medium and slime cigarettes. For aldehydes and ketones (except 2-heptanone, 2-methyloxolan-3-one and 6-methyl-3,5-heptadien-2-one) with boiling points ranged from 133 to 215 °C, such as 2-cyclopentene-1-one, 2-methyl-2- cyclopentene-1-one and furfural, their releases were higher from the medium cigarettes than those from the slim and king-sized cigarettes. [ABSTRACT FROM AUTHOR]

In order to measure the contents (mass fraction) of Amadori compounds in flue-cured tobacco leaves from various ecoregions, 249 tobacco leaf samples from 8 flue-cured tobacco ecoregions with different flavor types were selected and their contents of Amadori compounds in leaves were analyzed. Correlations of the Amadori compounds with chemical quality indexes from different ecological regions were determined. The results showed that: 1) The contents of total Amadori compounds in leaves from different stalk positions in the 8 ecoregions were in the order of upper leaves>middle leaves>lower leaves, all of which were normally distributed, and the content of Fru-Pro was the highest. 2) Most Amadori compounds in the flue-cured tobacco were positively correlated with leaf sensory properties. 3) According to heat map analyses, the 8 ecoregions in the order of the contents of Amadori compounds were Region VIII > Region VI > Region V > Region I > Region II > Region III > Region IV >Region VII. The contents of Fru-Pro, Fru-Gln and Fru-His in the leaves from southwest plateau ecoregion-fresh sweet flavor type (Region I) were higher. Among them, Fru-Pro content significantly negatively correlated with nitrogen/nicotine ratio; the contents of Fru-Phe, Fru-Ser, Fru-Val, Fru-Thr and Fru-Leu extremely significantly negatively correlated and the contents of Fru-Tyr, Fru-Ile and Fru-Aba significantly negatively correlated with potassium/chlorine ratio. [ABSTRACT FROM AUTHOR]

To improve the solution efficiency of coupled heat and moisture transfer model for porous media, the tridiagonal matrix algorithm (TDMA) was adopted to solve the model, and the comparisons between the TDMA and the iteration method were conducted. The sensitivity analysis between the TDMA calculation error and the time increment was conducted. Based on the sensitivity analysis results, a variable time increment TDMA optimization algorithm was proposed. The numerical results show that for cases with variable properties, the calculation accuracy of TDMA decreases while the accuracy of the iterative method remains unchanged with the increase of time increment. For the cellulose insulation, the effects of time increment on the calculation accuracy of TDMA can be ignored when the temperature and the relative humidity variations in two successive time steps are less than 0.1 °C and 0.24%, respectively. Compared with TDMA, the variable time increment TDMA algorithm is insensitive to time increment, showing a higher calculation accuracy. Compared with the iteration method, the variable time increment TDMA algorithm has the same calculation accuracy, but the calculation time is shorter, which is reduced by 67 %. [ABSTRACT FROM AUTHOR]

In order to investigate whether the pH of total particulate matter (TPM) can represent the acid-base balance of mainstream cigarette smoke, and to analyze the correlations between the TPM pH and the chemical components of mainstream cigarette smoke, a method for determining the TPM pH was developed. The correlations between the releases of the routine chemical components, plus 30 main acidic components, 40 main alkaline components and ammonia in mainstream smoke and the TPM pH of cigarette samples made of different single grade tobacco leaves and commercial cigarette samples were analyzed. The influences of adding acidic and alkaline components to cigarettes on the TPM pH were investigated. The results showed that: 1) Adding 1% (mass fraction) sodium chloride to isopropanol aqueous solution increased the differences of detected values of TPM pH among different samples and reduced the relative standard deviation of the measurement values. 2) The correlations between the releases of routine components, the main acidic components, the main alkaline components and ammonia in mainstream smoke and the TPM pH could not be verified on the two types of cigarette samples. 3) The ratios of nicotine release to the total release of acidic components, the total release of carboxyl units or the total release of hydrogen ions in mainstream cigarette smoke significantly correlated to the TPM pH, which could be verified on the different cigarette samples of the two types. 4) The contributions of 2-furoic acid, formic acid, glycolic acid and lactic acid to the total release of hydrogen ions in mainstream cigarette smoke were relatively higher; compared with the other acidic components in the smoke, the effects of these four acidic components on the TPM pH were greater at the same adding mass. The influence of adding ammonia on the TPM pH was stronger than that of nicotine. [ABSTRACT FROM AUTHOR]

In order to solve the challenge that strong polar analyte protectants are not well performed in weak polar solvent systems, the solution of acetonitrile : dichloromethane=2 : 1 (V/V) was chosen as weak polarity solvent system. The effects of 23 compounds as analyte protectants on the heights and shapes of chromatographic peaks of 31 aroma components were investigated. The chromatographic peaks of the aroma components in solvent added with these analyte protectants were compared with those in tobacco matrix. After optimizing the type, composition and concentration of the protectants, a suitable combination of analyte protectants was developed. In addition, 15 aroma components in tobacco (other than the 31 aroma components mentioned above) were chosen to verify the matrix compensation effect of the developed analyte protectant combination. The results showed that: 1) The suitable analyte protectants combination was malic acid (1 000 mg/L) + 1, 2-tetradecanediol (1 000 mg/L) . 2) By adding the optimized analyte protectants combination into the solvent standard solution, a satisfactory matrix compensation effect was achieved. The linearity of the calibration curve for each aroma component was greatly improved, and the slope and linear correlation coefficient (r²) were basically consistent with the matrix-matched calibration curve. 3) The matrix effect of aroma components in tobacco during GC/MS analysis was compensated by adding analyte protectants. [ABSTRACT FROM AUTHOR]

In this paper, copper oxide nanorods (CuO-NRs) prepared by uniform precipitation method were combined with graphene (GNs) slurry, and CuO-NRs/GNs composite counter electrodes were prepared on FTO conductive glass substrated by electrohydrodynamics (EHD). The XRD phase analysis and SEM, TEM structure characterization indicated that the CuO-NRs/GNs composite film had a porous network structure. It proved that the CuO-NRs/GNs composite counter electrode had high electrocatalytic activity and faster electron transport rate through a series of electrochemical performance tests, and achieved photoelectric conversion efficiency of DSSC assembled by CuO-NRs/GNs composite counter electrodes (2.57%) reached the same level as that of Pt counter electrodes (3.46%). [ABSTRACT FROM AUTHOR]

The community home care has increasingly become the mainstream choice of the old-age care model in China. For a long time, the fragmentation of caring, nursing and medical care has restricted the development of old-age care services. Essentially, it is a coherent system with inherent logic coupling. Maintenance is the norm, care is the essence, and medical care is the guarantee. The rise of smart communities has provided opportunities for community care. The introduction of smart technologies has improved the accuracy of old-age care services, played a catalyst and bridge role in building a refined old-age service system, and promoted the convergence of factors, but also brought the digital divide, privacy leaks and technical panic. To this end, it is necessary to combine top-down administrative governance with bottom-up interaction and innovation, making use of the role of assessment and accountability mechanisms to stimulate consensus and coherence among all parties, rationally and appropriately apply smart technology to provides medical care for the trinity of old-age care services and ultimately achieving the goal of smart old-age care. [ABSTRACT FROM AUTHOR]

In order to increase the production of low pressure, low permeability, strong water locking or water sensitive damaged tight sandstone reservoir after fracturing, research was conducted on CO2 dry fracturing fluid system. The molecular simulation technique was used to study the microscopic configuration of molecules between liquid CO2 and viscosifier, to evaluate the viscosifying effect of various CO2 viscosifer on liquid CO2, to optimize the viscosifiers, and to build the formula for CO2 fracturing fluid. Based on the research, it was found that, under the conditions of 62-63 t and 15-20 MPa, the viscosity of CO2 dry fracturing fluid system was increased to 5-10 mPa · s, which is 240-490 times that of supercritical CO2. The average rate of damage to core permeability of gas wells was 2.75%, and the average rate of damage to core permeability of oil wells was 0.98%. This fracturing fluid system was successfully tested on the first domestic well with CO2 dry sand fracturing in Sulige Gas Field, and the production was increased remarkably. [ABSTRACT FROM AUTHOR]

The article examines the situation of Chinese long sentence query based on Large-scale search logs. It cites the relationship between the user queries search behavior and query length, types and frequency which features the length of query modification and disciplines. It also notes that the long query can see the user's query terms features than the short query and mentions it role in query technology improvements and construction.

Complex odontoma is a relatively rare dental dysplasia. In particular, a complex odontoma with dentigerous cyst is seldom observed. A case of complex odontoma with dentigerous cyst is reported in this paper. [ABSTRACT FROM AUTHOR]

Aroma is one of the important indexes to measure the organoleptic quality of fruit wine. However, the weak aroma restricts the further improvement of fruit wine quality. The aroma enhancement of fruit wine is one of the focuses of attention for relevant practitioners. However, the complicated mechanism of aroma regulation is a bottleneck problem that restricts the creation of aroma-enhancing techniques for fruit wine fermentation. In this paper, we provide a systematic review of the mechanism of the influence of starter cultures, enzymes, and pretreatment before fermentation on the aroma of fruit wine with regard to the types of key aroma compounds and their formation pathways, and we discuss the application of modern biotechnology in the breeding of excellent aroma producing yeast. Finally, we discuss future directions in the research on fruit wine flavor.

Objective To investigate the effect of different decontamination methods, including photodynamic therapy, sandblasting and titanium curette, on titanium surface morphology and bacterial adhesion for the treatment of peri-implant disease. Methods Porphyromonas gingivalis (Pg) and Fusobacterium nucleatum (Fn) were inoculated on the surface of polished titanium specimens, and titanium specimen surfaces were treated with different decontamination methods after incubation. The titanium specimens were divided into a no-treatment control group, photodynamic group, sandblasting group and titanium curette group according to different decontamination methods. The changes in titanium surface roughness were observed by atomic force microscopy (AFM), and the remaining bacteria on the titanium surface were observed by scanning electron microscopy (SEM) and live/dead bacteria staining tests. After reinoculation of Pg and Fn, bacterial readhesion was observed on the surface of decontaminated titanium specimens. Results The AFM results showed that the surface roughness of the titanium curette group was significantly higher than that of the no-treatment control group, photodynamic group and sandblasting group (P0.05). The results of contact angle measurement showed that the surface contact angle of each treatment group was smaller than that of the no-treatment control group (P

Objective To investigate the biomechanical effects of upper lip pressure on the maxilla in patients with a unilateral alveolar cleft and provide evidence for clinical diagnosis and treatment. Methods A 3D finite element maxillary model was generated based on cone beam CT (CBCT) data from an 11-year-old female patient with a unilateral alveolar cleft. Two different kinds of upper lip pressure, postsurgery pressure and normal pressure, were applied to the model. The displacement and stress of each reference node were compared and analyzed. Results By loading upper lip pressure, the maxillary alveolar crest rotated toward the defect and was displaced downward and backward. The displacement of the noncleft side was greater than that of the cleft side and it decreased gradually from the anterior to the posterior. The stress was concentrated on the anterior portion of the alveolar crest. The stress on the noncleft side was greater than that on the cleft side and it decreased gradually from the anterior to the posterior. The maximum stress was concentrated on the palate around the defect. The displacement and stress in the postsurgery group were greater than those of the normal group (P

Objective To explore the diagnostic value and clinical relevance of antibodies against aquaporin-1 (anti-AQP1) and aquaporin-5 (anti-AQP5) in primary Sjögren's syndrome (pSS), and investigate new potential biomarkers that can contribute to the diagnosis. Methods A prospective cohort trial was conducted on 70 patients with pSS (pSS group), 67 patients with other connective tissue diseases and 39 healthy individuals admitted to Department of Rheumatology and Immunology of our hospital from September 2021 to March 2022. The patients with other connective tissue diseases and healthy individuals were assigned into the control group. The contents of anti-AQP1 and anti-AQP5 were measured by enzyme linked immunosorbent assay (ELISA). Meanwhile, EULAR Sjögren's syndrome disease activity index (ESSDAI) score, and levels of anti-nuclear antibody, anti-SSA antibody, anti-SSB antibody, anti-Ro52 antibody, ds- DNA, rheumatoid factor and fodrin were collected and analyzed with Chi-square test for correlation. Results No statistical difference was observed in the level of anti-AQP1 between the pSS group and control group (P>0.05), but the level of anti-AQP5 was significantly higher in the pSS group than the control group [26.41 (24.05, 28.08) vs 7.63 (5.49, 10.29) ng/mL, P < 0.05]. ROC curve analysis showed that the area under the curve of anti-AQP1 was 0.523 (P>0.05), with a sensitivity of 0.371 and a specificity of 0.726. While, the area under the curve of anti-AQP5 was 0.915 (P < 0.05), with a sensitivity of 0.957 and a specificity of 0.821. The anti-AQP5 level was positively correlated with the levels of anti-SSA antibody, anti-SSB antibody, anti-Ro52 antibody, anti-nuclear antibody and rheumatoid factor (P < 0.05), but had no correlation with ESSDAI score in the pSS patients (P>0.05). Conclusion The serum level of anti-AQP5 is significantly increased in pSS patients, with a sensitivity of 95.7% and a specificity of 82.1%, and is associated with clinical practice and of high potential diagnostic value.

ObjectiveTo investigate the effects of diabetes on tumor progression, and the effects of diabetes combined with colorectal or breast cancer on the intestinal flora by constructing a diabetes-tumor mouse model, and the differences in body mass, tumor volume, survival rate, and initial flora of the mice in different treatment groups were statistically analyzed.MethodsSixty mice were divided into control, diabetes, colorectal cancer, diabetes + colorectal cancer, breast cancer, and diabetes + breast cancer groups. The diabetic mouse model was established by streptozotocin injection, and solid tumor models of colorectal and breast cancer were further established on this basis. Changes in the body mass, tumor volume, and survival rate of the mice in each group were observed and analyzed. The mouse feces were collected, and then the microbial community sequencing data were analyzed using the QIIME2 platform based on the DATA2 and Vesearch methods.ResultsThe body mass (P < 0.05) and survival rate (P < 0.01) of mice with diabetes combined with colorectal or breast cancer were significantly reduced, but there was no significant difference in tumor volume in mice. Compared with the control group or the colorectal cancer group, the species richness of the intestinal flora in mice in the diabetes + colorectal cancer group was significantly altered (P < 0.05); while the diabetes + breast cancer group did not significantly change (P > 0.05). The beta diversity and bacterial species composition of the intestinal flora in mice with diabetes combined with colorectal or breast cancer were also affected.ConclusionDiabetes promotes the progression of colorectal or breast cancer. Diabetes combined with colorectal or breast cancer has a greater impact on the intestinal flora of mice than colorectal or breast cancer alone.

[Purpose/Significance] Science funding provides financial support for academic institutions, researchers and research projects, and at the same time plays a guiding role in the development of scientific innovation. The research of funded papers contributes to the development of the discipline and is of great significance to the encouragement and support of researchers. [Method/Process] Based on the funded papers published in Chinese journals and foreign journals in the field of library and information science (LIS) during the "13th Five-Year Plan" period, this paper analyzes from the perspectives of the time distribution of papers issued, research output of different types foundations and the research topics. [Results/Conclusions] The number of funded papers published in Chinese journals in LIS from 2016 to 2020 has decreased year by year, but the number of funded papers published in foreign journals has slowly increased year by year; for funded papers published in Chinese journal during the "13th Five-Year Plan" period, provincial (municipal) foundations funded papers are more outstanding, and National Natural Science Foundation funded papers are more notable for the number and effects of the funded papers in foreign journals; the funded papers in Chinese journals show a great impact on library services, changes and data, digital resource construction and university libraries; while funded papers in foreign journals pay more attention to social media, theme models, scientometrics and library construction.

[Introduction] Due to the tendency of distributed photovoltaic power generation projects becoming more and more popular on the Internet, it is more and more important for the optimal design of various aspects of photovoltaic power generation projects. Based on a rooftop distributed PV power generation project in Shandong Province. [Method] This paper optimized the design of bracket inclination, component arrangement and bracket foundation selection. Through PKPM modeling and calculation, the paper emphasized on material usage and economy. [Result] The results show that when the concrete base weight is 2.4 m, the steel cost is 2.4 m, 2.8 m and 3.2 m, the total cost can be reduced by 0.55%, 27.5% and 37.9%. [Conclusion] We demonstrate that the steel volume, the concrete dosage and the cost are all optimized when the distance between the two columns is 2.4 m.

To study the influence of different bearing clearance on the angular transmission error of RV reducer,the characteristics of the angular transmission error as the combination forms of different bearing clearances are obtained through applying dynamics simulation work on a simulation model of RV reducer,which containing cycloidal gear profile modification,bearing clearance and nonlinear contact of gears. By analysis of orthogonal test on the basis of above,the sensitivity and influence law of the different bearing clearance on the angular transmission error of RV reducer are analyzed. Furthermore,the bearing clearance can be designed targeted by dimension tolerance control,which provides a useful reference for the design and manufacture of RV reducer.

The tritium breeding blanket (TBB) technology is one of the key technologies to be solved and also one of three major issues in the development of fusion reactor in the future. The international thermonuclear experimental reactor (ITER) will be the first nuclear fusion experimental reactor in the world whilst ITER test blanket module (TBM) program is the only way to test and validate the tritium breeding blanket technologies under a real fusion environment at present. The successful implementation of ITER TBM program will provide a solid scientific and engineering foundation for fusion energy development. This paper briefly introduces the content, necessity, strategy, organizing and implementation of ITER TBM program, and summarizes the development status and achievement of test blanket system (TBS) made by the participants of ITER members. Prospects of the TBS research activities for the next phase and subsequent research focuses are presented. It is expected to provide valuable references for tritium breeding blanket design and development of China fusion engineering test reactor (CFETR), as well as the development of the TBB technologies.

In order to study the influence of modification of cycloid gear and bearing clearance on angular transmission error of RV reducer,based on the multi-body dynamics simulation technology,multi-body contact between gears is established by combining with the most advanced mixed contact checking algorithm rely on relative coordinate contact configuration space method and boundary box method,furthermore the spring force element is used to eliminate the redundant constraints in the dynamic simulation model,at the same time the bearing clearance is introduced,at last the dynamic simulation model of RV reducer containing the cycloid gear modification and bearing clearance is established. Then the angular transmission error of RV reducer under the condition of special tooth profile modification of cycloid gear and different quantity of bearings clearance are checked by multi-body dynamics simulation,which providing a new modeling ideas and methods for studying the effect of cycloid gear profile modification and bearing clearance on angle transmission of RV reducer with multi-body dynamics simulation.

Based on i SIGHT multidisciplinary optimization platform integrated finite element analysis software ABAQUS,the sub-model technology,global optimization strategy,hybrid iterative optimization algorithm and parametric modeling method of spline curve were used in optimization design of sealed-module structures,which greatly reduced the single-solving time and improved the efficiency of entire optimization process. The weight and stress of sealed-module structures are reduced significantly after optimized and the optimization result are verified by hydrostatic test. This method solves a problem of high stress of sealedmodule and provides a referenced technical route for optimization design of sealed-module in future.

Objective: To summarize clinical predictors and imaging characteristics of critically ill children infected with SARS-CoV-2 Omicron with neurological complications in Shenzhen during the peak of the first round of infections., Methods: The clinical data of 11 critically ill children with neurological complications infected with SARS-CoV-2 Omicron in Shenzhen Children's Hospital from December 12 to 31, 2022, were retrospectively collected and analyzed. Laboratory test results related to liver parenchymal injury, histiocytic injury, inflammation, and coagulation function were collected, and imaging characteristics including CT and/or magnetic resonance imaging (MRI) were analyzed. The differences in CT/MRI score, acute necrotizing encephalopathy severity scale (ANE-SS) score and total score (CT/MRI score + ANE-SS score) were compared between the two groups with different prognosis during hospitation., Results: Among 11 children, 7 were male and 4 were female. The age ranged from 10 months to 16 years. There were 5 cases of acute necrotizing encephalopathy (ANE) and 6 cases of acute fulminant cerebral edema (AFCE). During hospitalization, 3 patients survived and 8 patients died of multiple organ dysfunction syndrome (MODS), including 2 cases of ANE and 6 cases of AFCE. All cases had fever (> 38.5 centigrade), and 3 cases had ultra-high fever (> 41 centigrade). Within 48 hours of onset, all cases had disorders of consciousness and 9 cases had seizures. The 8 dead children had complications with multisystem involvement, including shock, respiratory failure, disseminated intravascular coagulation (DIC), liver failure, renal failure or myocardial damage, and the laboratory predictors related to hepatocellular injury [alanine aminotransferase (ALT), aspartate aminotransferase (AST)], histocyte injury [creatine kinase (CK), lactate dehydrogenase (LDH)], inflammation [procalcitonin (PCT), interleukin-6 (IL-6), serum ferritin (SF)], coagulation function (D-dimer) and blood glucose (Glu) increased in different quantities, of which PCT was specifically increased in 6 cases with AFCE, PLT was specifically decreased in 3 cases with AFCE, and ALT and LDH were significantly increased in 2 cases with ANE. Imaging analysis showed subarachnoid hemorrhage, basal ganglia and thalamus lesions in all 6 cases with AFCE, while thalamus lesions in all 5 cases with ANE. The ANE-SS score of 8 deceased children ranged from 2 to 7 (of which 6 cases were ≥ 5), and the ANE-SS score of 3 surviving children ranged from 0 to 2. Eight dead children had a CT/MRI score of 1-4 (of which 6 cases were 4), and 3 surviving children had a CT/MRI score of 1-2 (of which 2 cases were 1). The total score of 8 deceased children was 6-10 (of which 6 cases ≥ 8), and 3 surviving children was 1-4., Conclusions: The neurological complications of critically ill children infected with SARS-CoV-2 Omicron in Shenzhen progressed rapidly to ANE and AFCE, with high mortality. High fever (> 40 centigrade), convulsion/disturbance of consciousness, and multiple organ failure were the most common symptoms in ANE and AFCE cases. PCT increased and PLT decreased specifically in AFCE cases. Poor prognosis (death) was more common in age < 4 years old, predictors of ALT, AST, CK, LDH, PCT, D-dimer, Glu, IL-6 increased significantly, PLT decreased significantly. The common imaging feature of ANE and AFCE is the involvement of dorsal thalamus, a new imaging sign of AFCE (subarachnoid hemorrhage) was found. The higher the ANE-SS score, CT/MRI score and total score, the greater the risk of death.

Objective: To investigate the prospective association between sugar-sweetened beverage intakes and age at menarche among Chinese girls., Methods: Based on China Health and Nutrition Survey(CHNS) from 2004 to 2015, 293 girls aged 7-16 years with both data on sugar-sweetened beverage intake 1-5 years before menarche and age of menarche were included in the present study. Multivariate linear regression analysis and Logistic regression analysis were used to explore the influence of the sugar-sweetened beverage consumption on menarcheal age of Chinese girls., Results: The median age at menarche of girls was 12 years old. The consumption of sugar-sweetened beverages(SSBs) was 0.5(0.2, 1.0) L/week for sweetened soft drinks, 0.5(0.2, 0.9) L/week for sweetened fruit drinks and 0.6(0.3, 1.3) L/week for total. After adjusting age at baseline, residency, maternal educational, level, energy intake at baseline and body mass index standard deviation score, no significant association was found between sweetened soft drinks, sweetened fruit drinks, total sugar-sweetened beverage intake and age at menarche., Conclusion: The intake of sugary beverages may not be prospectively related to the age of menarche among Chinese girls.

Bacteria are often infected by large numbers of phages, and host bacteria have evolved diverse molecular strategies in the race with phages, with abortive infection (Abi) being one of them. The toxin-antitoxin system (TA) is expressed in response to bacterial stress, mediating hypometabolism and even dormancy, as well as directly reducing the formation of offspring phages. In addition, some of the toxins' sequences and structures are highly homologous to Cas, and phages even encode antitoxin analogs to block the activity of the corresponding toxins. This suggests that the failure of phage infection due to bacterial death in abortive infections is highly compatible with TA function, whereas TA may be one of the main resistance and defense forces for phage infestation of the host. This review summarized the TA systems involved in phage abortive infections based on classification and function. Moreover, TA systems with abortive functions and future use in antibiotic development and disease treatment were predicted. This will facilitate the understanding of bacterial-phage interactions as well as phage therapy and related synthetic biology research.

Objective: To review the research progress on the role of Schwann cells in regulating bone regeneration., Methods: The domestic and foreign literature about the behavior of Schwann cells related to bone regeneration, multiple tissue repair ability, nutritional effects of their neurotrophic factor network, and their application in bone tissue engineering was extensively reviewed., Results: As a critical part of the peripheral nervous system, Schwann cells regulate the expression level of various neurotrophic factors and growth factors through the paracrine effect, and participates in the tissue regeneration and differentiation process of non-neural tissues such as blood vessels and bone, reflecting the nutritional effect of neural-vascular-bone integration., Conclusion: Taking full advantage of the multipotent differentiation ability of Schwann cells in nerve, blood vessel, and bone tissue regeneration may provide novel insights for clinical application of tissue engineered bone.

Object Analyze the adverse events of bakri postpartum balloon in order to promote its safety. Methods Collect and analyze the adverse events from the National Medical Device Adverse Event Monitoring Information System and the U.S. FDA MAUDE database. Chinese and English instructions for use are comparative analysed. Results In most cases, adverse events are balloon rupture and fluid leakage, which attribute to insufficient instruction. The description of the sequence that suturing the myometrium and filling the balloon is deficient. It should be moved gently to avoid puncturing or scratching the balloon with sharp instrument. Conclusion The product instruction improvement as well as training enhancement is recommended for safety consideration. Adverse events monitoring is suggested reinforce simultaneously.

Phthalates esters (PAEs) are important organic compounds used as plasticizers to enhance their plasticity and versatility. At the same time, PAEs are also typical environmental endocrine disruptors. Long-term production and use of plastic products have caused harm to the environment and organisms, as well as human health. Previous studies found that biodegradation has become a main pathway to reduce PAEs in the environment. This article reviews PAEs' structural characteristics and classifications, toxicology, pollution in the environment, summarizes the diversity of PAEs-degrading bacterial species, and explores the possible mechanisms of bacterial PAEs degradation. The article may provide some reference in solving the problem of PAEs pollution.

Objective: To investigate the effectiveness of levator muscle resection combined with Mustarde's double Z-plasty to correct blepharophimosis-ptosis-epicanthus inversus syndrome (BPES)., Methods: Between March 2015 and June 2017, one-stage operation of levator muscle resection combined with Mustarde's double Z-plasty were performed on 26 children with bilateral BPES. There were 16 boys and 10 girls with an average age of 7 years (range, 4-14 years). All patients marked the four typical signs of BPES. There were 7 cases accompanied with a low nasal bridge, and 20 cases with amblyopia and strabismus. The length of eye fissure was (19.5±4.5) mm, the width of eye fissure was (2.5±1.6) mm, the diameter of inner canthus was (42.1±6.5) mm, and the muscular strength of levator palpebrae superioris was (5.5±1.3) mm., Results: All the incisions healed by first intention. Twenty-three patients were followed up 2-12 months, with an average of 10 months. Among which, 2 cases were less corrected, 3 cases were over corrected, 6 cases had poor curvature of the eyelid. No eyelid internal and external pronation or keratitis occurred. Amelioration of blepharoptosis and epicanthus was achieved in the other patients, and the double eyelid fold was naturally smooth. At 7 days after operation, the length of eye fissure was (27.2±1.9) mm, the width of eye fissure was (12.5±1.3) mm, and diameter of inner canthus was (29.4±2.6) mm, which were superior to preoperative values ( t =0.127, P =0.042; t =0.341, P =0.029; t =0.258, P =0.038). There was no angular deformity caused by the width and length regressions of eye fissures., Conclusion: The levator muscle resection combined with Mustarde's double Z-plasty can effectively correct BPES and obtain good effectiveness.

Xylanase is a high-profile glycoside hydrolase with applications in brewing, feed, pharmacy and bioenergy industries, but most of xylanases are in active below 30 ℃. In order to obtain low temperature active xylanase, a xylanase gene, XYN11A, was cloned from Penicillium sp. L1 and expressed in Pichia pastoris GS115. After purification and enzyme assay, optimal pH and temperature were determined to be 3.5 to 4.0 and 55 ℃. This enzyme was stable at acid and neutral condition (pH 1.0 to 7.0) or under the treatment of 40 ℃ for 1 hour. This xylanase displayed strong resistance to all tested ions and chemicals. Noteworthily, XYN11A maintained a higher activity of 6 700 U/mg than a lot of GH11 xylanase, and demonstrated higher activity (24% to 58%) at lower temperature from 20 to 40 ℃. After beechwood xylan hydrolysis for 16 h, the hydrolysates consisted mainly of xylobiose, xylotriose and xylotetraose and barely of xylose, thus XYN11A could be used for the production of prebiotic xylooligosaccharide. Possessing the features of acidophilic, highly active at lower temperature and oligosaccharide production, XYN11A demonstrated great potential in food and feed industrials.

Objective: To judge whether algesia sensitization of some acupoints is existed and whether the acupoint algesia sensitization area is expanded in the patients of intestinal cancer., Methods: Totally, 30 patients of intestinal cancer and 30 healthy subjects were included. The electronic Von Fray was used to determine the pressure-pain thresholds at 13 acupoints relevant with gastrointestinal disorders and the reference points at the sites 1 cun and 2 cun lateral to those points as well as the sites at the corresponding nerve segments. Compared with the pressure-pain thresholds at the reference points of the different segments, the relative value was calculated. The changes were analyzed in the pressure-pain thresholds at the relevant acupoints on the body surface in the patients of intestinal cancer as compared with the relative pressure-pain thresholds in the healthy volunteers., Results: The pressure-pain thresholds at Zusanli (ST 36), Shangjuxu (ST 37), Xiajuxu (ST 39), Quchi (LI 11) and Dachangshu (BL 25) in the patients of intestinal cancer were all significantly reduced as compared with those of the healthy subjects ( P <0.05, P <0.01, P <0.001). At the non-acupoint sites 1 cun and 2 cun lateral to those acupoints as well as at the sites of the same segments, the pressure-pain thresholds were reduced significantly as compared with the control group ( P <0.05, P <0.01, P <0.001). Particularly, the sensitization zone of Yinlingquan (SP 9) focused on the acupoint, the site 1 cun lateral to it as well as the non-acupoint sites of the same segments ( P <0.01, P <0.001)., Conclusion: The acupoint sensitization is displayed at Zusanli (ST 36), Shangjuxu (ST 37), Xiajuxu (ST 39), Quchi (LI 11), Dachangshu (BL 25) and Yinlingquan (SP 9) and the sensitization area is expended in the patients of intestinal cancer.