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Objective To investigate the functions of the KIFC1 gene in tumor cells and its effect on the proliferation of cervical cancer cells. Methods We designed sgRNAs targeting the KIFC1 gene and constructed a recombinant plasmid based on the pSpCas9 (BB)-2A-GFP vector, which was co-transfected into HeLa cells. We screened monoclonal knockout cell lines through flow cytometry sorting, limited dilution inoculation of cells, and sequencing. RT-qPCR, Western blot, and immunofluorescence were used to detect the transcription and protein expression levels of KIFC1 in knockout cells. Cell phenotypes such as nucleus and microtubule cytoskeleton were observed using phase-contrast microscopy and fluorescence confocal microscopy. Cell proliferation, cell cycle, and apoptosis were analyzed by growth curve plotting, EdU labeling, and acridine orange staining. Results The deletion of the KIFC1 gene resulted in the abnormal phenotypes of HeLa cells, with increased numbers of multinuclei, micronucleus, and disordered microtubules. The cell cycle was disrupted, accompanied with a significant increase in the ratio of late apoptotic cells and a decrease in cell proliferation (all P < 0.05). Conclusion KIFC1 gene deletion affects the assembly of microtubules and cell division in HeLa cells, leading to abnormal nuclear morphology, chromatin elimination, cell cycle arrest, and increased cell apoptosis.

Ghrelin, a newly discovered gastric peptide, involves in the regulation of various biological processes and plays important role in the occurrence and development of metabolic diseases such as non-alcoholic fatty liver disease, diabetes and obesity by effectively regulating glucose and lipid metabolism and anti-oxidation.

Investment is an important way for regions to optimize their economic structure and participate in globalization. With the evolution of the world's political and economic environment, investment has become an important link to national competition. Including a big power game element is logical to identify investment risks. Simultaneously, in the context of the increasing scale of China's outbound investment, research on investment risk has focused more on identifying the host country's investment environment. In contrast, the impact of significant country-specific competition factors on national investment has yet to be explored. Given this background, this paper adds the concept of geopotential with space power as a measurement factor to the traditional investment environment risk evaluation and establishes a regionally integrated investment risk identification model from the perspective of great power competition. Taking Southeast Asia as an example, we measure the basic investment environment risks of Southeast Asian countries and changes in the geopolitical positions of China and the United States in Southeast Asia. Based on the above, we identify changes in China's investment risks in Southeast Asia in the competition between China and the United States. The results show the following: 1) The basic investment environment risks of Southeast Asian countries show apparent spatial heterogeneity, and the spatial structure is high in the north and low in the south, with Singapore and Malaysia as stable low-risk areas and Myanmar and Laos as stable high-risk areas. 2) The geopolitical positions of China and the United States from 2010 to 2019 show significant regional heterogeneity. The United States has geopolitical advantages in most regions; however, the gap between China and the United States has gradually narrowed. China's geopolitical positions in Myanmar and Laos are ahead of those in the United States. Moreover, the geopolitical positions of China and the United States in Vietnam and Cambodia show intertwined changes. The geopolitical positions of China and the United States in Singapore, the Philippines, Malaysia, Indonesia, Brunei, and Thailand show a parallel evolution. 3) Significant interregional differences in integrated risks based on geographical potential and basic investment environment risks were observed. The spatial distributions of high- and low-integrated risk areas are stable, with Thailand and the Philippines as stable high-risk areas, Myanmar, Laos, and Vietnam as stable low-risk areas, and medium-integrated risk areas showing expansion to the south of the region. 4) According to kernel density estimation, the spatial gap between China's integrated investment risks in Southeast Asia is narrowing, and a non-polarized development of evolutionary characteristics is observed.

Isocitrate dehydrogenase (IDH), a key enzyme in the tricarboxylic acid cycle, plays an important role in cellular energy metabolism. When the IDH gene is mutated, the enzyme activity is altered, resulting in an accumulation of a large amount of the tumor metabolite 2-hydroxyglutaric acid (2-HG), causing severe epigenetic deregulation and dysregulation of gene expression, and promoting tumorigenesis. Recent studies have shown that IDH1 and IDH2 mutations are closely related to the occurrence and development of a variety of tumors such as glioma, acute myeloid leukemia(AML) and intrahepatic cholangiocarcinoma(iCCA) as well as their clinical treatment. It is of great significance to use IDH gene mutations as molecular indicators for detecting glioma, AML and iCCA tumorigenesis, and developing targeted drugs. This review is focused on the mechanism of IDH mutation, the relationship between mutant IDH and the development of multiple tumors, and the progress of treatment of IDH mutation in basic research and drug clinical trials.

A simple and sensitive fluorescent probe for the determination of phalic acid ester (PAEs) in fermented foods was developed based on the fact that the oxidase-like activity of Cu,Fe-N-C nanozyme can be regulated by PAEs. After PAEs were adsorbed on the surface of Cu,Fe-N-C, the catalytic oxidation of the substrate o-phenylenediamine (OPD) was accelerated, thus increasing the fluorescence intensity of the generated 2,3-diaminophenazine (DAP). The calibration curve for PAEs showed a good linear relationship in the concentration range of 1.25–50 μg/L. The detection limit of the proposed method was 0.76 μg/L. The recoveries of spiked samples ranged from 88.0% to 104.6%, which meet the detection requirements of the national standard GB 5009.271-2016. This method is simple, stable and rapid, and its detection limit is lower than the national limit for PAEs content in foods.

Aiming at assisting the color design of cultural creative product and outputting color schemes that correspond to multi-user image preference effectively, an interactive color design method driven by group consensus was proposed in this paper. Firstly, the triangular fuzzy number was used to quantitatively descript users' image preference and a consensus degree model of group users' image preference was constructed. By studying the process and basic principles of interactive genetic algorithms, the interactive evolution operation for cultural creative product color design was then implemented based on the group consistency of decision-making combined with the triangular fuzzy number, which can help generate cultural creative product color schemes that satisfy group consensus and satisfaction. Next, the color aesthetic measure model was used to rank and select the final product color design schemes. Finally, taking the color design of terracotta warriors' statue as an example, it is verified that the proposed method can effectively integrate the image preference of group users, and assist designers to better conduct cultural creative product color design with the consistent results of multi-user decisions.

The first-principle pane wave pseudopotential method in GGA-PBE generalized gradient approximation is aphid to study the lattice parameters, electronic properties and mechanical properties of hydrogen storage alloy LaNi4Ge under different pressures. The results show that the lattice parameters of LaNi4Ge compound decrease with the increase of pressure, and is a-axles is more easily compressed than taxes. There are no signal cant trend changes in the total density of states, the Fermi energy level decreases with the increase of pressure, and is hardness increases with the increase of pressure. The elastic properties show that the material is decisive in the pressure range, and the elastic constant Cu and elastic modulus E increase further with the increase of pressure, and maintain the mechanical stability in a certain pressure range. [ABSTRACT FROM AUTHOR]

Objective: To explore the genetic etiology of a child with Nemaline myopathy (NM)., Methods: A child who had visited Fujian Children's Hospital on January 28, 2023 due to "phlegm in the throat for more than a month" was selected as the study subject. Clinical data of the child was collected, in addition with peripheral blood samples from her and her parents. Following extraction of genomic DNA, trio-whole exome sequencing (WES) was carried out. Candidate variants was verified by Sanger sequencing and bioinformatic analysis. This study has been approved by the Medical Ethics Committee of Fujian Children's Hospital (Ethic No. 2023ETKLRK2004)., Results: The patient, a 2-month-old female, had presented with persistent phlegm in the throat, recurrent severe pneumonia, swallowing difficulty, and decreased muscle tone. WES results revealed that she has harbored compound heterozygous variants of the NEB (NM&#95;001271208.1) gene, namely c.4611+2T>A and c.12961del (p.Ser4321ALafs*21), and the associated disease is rod-like myopathy. Sanger sequencing confirms that the variants were respectively inherited from her mother and father, both of whom had normal phenotypes. Based on the guidelines from the American College of Medical Genetics and Genomics (ACMG), both variants were rated as likely pathogenic (PVS1&#95;Moderate+PM2&#95;Supporting+PM3; PVS1+PM2&#95;Supporting)., Conclusion: The c.4611+2T>A/c.12961del (p.Ser4321ALafs*21) compound heterozygous variants of the NEB gene probably underlay the pathogenesis in this child. Above findings has facilitated the diagnosis, genetic counseling, and guidance for reproductive decision of her family.

Objective: To explore the genetic basis for a child with congenital heart disease (CHD) and global developmental delay (GDD)., Methods: A child who was hospitalized at the Department of Cardiac Surgery of Fujian Children's Hospital on April 27, 2022 was selected as the study subject. Clinical data of the child was collected. Umbilical cord blood sample of the child and peripheral blood samples of his parents were collected and subjected to whole exome sequencing (WES). Candidate variant was verified by Sanger sequencing and bioinformatic analysis., Results: The child, a 3-year-and-3-month-old boy, had manifested cardiac abnormalities and developmental delay. WES revealed that he had harbored a nonsense variant of c.457C>T (p.Arg153*) in the NONO gene. Sanger sequencing showed that neither of his parents has carried the same variant. The variant has been recorded by the OMIM, ClinVar and HGMD databases, but not in the normal population databases of 1000 Genomes, dbSNP and gnomAD. Based on the guidelines from the American College of Medical Genetics and Genomics (ACMG), it was rated as a pathogenic variant., Conclusion: The c.457C>T (p.Arg153*) variant of the NONO gene probably underlay the CHD and GDD in this child. Above finding has expanded the phenotypic spectrum of the NONO gene and provided a reference for the clinical diagnosis and genetic counseling for this family.

Novel model systems have provided powerful tools for the research of human biology. Despite of being widely used, the conventional research models could not precisely describe the human physiological phenomenon. Organoids are three-dimensional multicellular aggregates derived from stem cells or organ progenitors that could differentiate and self-organize to recapitulate some specific functionalities and architectures of their in vivo counterpart organs. Organoids can be used to simulate organogenesis because of their human origin. In addition, the genomic stability of organoids could be well maintained during long-term amplification in vitro. Moreover, organoids can be cryopreserved as a live biobank for high-throughput screening. Combinatorial use of organoids with other emerging technologies (e.g. gene editing, organ-on-a-chip and single-cell RNA sequencing) could overcome the bottlenecks of conventional models and provide valuable information for disease modelling, pharmaceutical research, precision medicine and regenerative medicine at the organ level. This review summarizes the classifications, characteristics, current applications, combined use with other technologies and future prospects of organoids.

A method based on gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the determination of phthalic acid esters (PAEs) in soil. The soil sample was extracted by dichloromethane-acetone (1: 1, v/v) with an ultrasonic instrument, and the extract was cleaned up with a Florisil column. The identification and quantification of the analytes were carried out in the multiple reaction monitoring (MRM) mode after the GC separation on an HP-5MS column (30 m x 0.25 mm x 0.25 microm). The eight point calibration curves showed a good linearity for all the congeners (0.997 3 - 0.997 6). The limits of detection (LODs) ranged from 0.1 to 0.5 microg/kg. The relative standard deviations (RSDs) were not more than 14.3% and the spiked recoveries were in the range of 72.9% - 106.2%. The developed analytical method is rapid and accurate, and suitable for the determination of PAEs in soil.