Search

Three-layer glue-free composite filmss ba,sed on a PP/EVA/ela,stomer blending,sy,stem were prepared through melt blending and ca,st co-extrusion using EVA a,s an adhe,sive layer and different easterners a,s a compatibilizer for the PP core layer and adhesive layer. The resultant blending system was analyzed by means of mechanical tessts, rheological tessts, and sscanning electron microsscopy, and the propertiess of the compossite filmss were inve,stigated. The ressuta indicated that there wa,s an optimal bonding effect on paper when using the EVA containing 18 wt% vinyl acetate. The PBE easterner exhibited a more ssignificant improvement in the mechanical properties* than POE. The glass transition temperature of PP and EVA increased gradually with an increase in the PBE content, and meanwhile the curvature of Cole-Cole also increased gradually, indicating that the compatibility between PP and EVA became better and better. When the content of PBE in the EVA adhesive layer was; 30 wt%, the tensile sstrength of the composite films; reached a maximum value of 29 MPa. The adhesive strength of the composite films with paper reached at maximum value of 9 N/15 mm at a PBE content of 20 wt%. The core layer and adhesive layer cannot be peeled off for the compossite filmss at a PBE content of 25 wt%. The compossite filmss are robust without delamination in thiss ca,se. The optimal loading of PBE in the adhe,sive layer wa,s determined to be 25 wt%. [ABSTRACT FROM AUTHOR]

Objective: To explore early effectiveness of unilateral biportal endoscopy (UBE) technique in the treatment of migrated lumbar intervertebral disc herniation., Methods: A retrospective analysis was conducted on 87 patients with migrated lumbar intervertebral disc herniation, who were treated with UBE technique between May 2021 and December 2022 and met the selection criteria. There were 55 males and 32 females, with an average age of 48.8 years (range, 29-74 years). The disease duration ranged from 2 to 23 months, with an average of 9.1 months. The surgical segments included 17 cases of L 3, 4 , 32 cases of L 4, 5 , and 38 cases of L 5 , S 1 . According to Lee's classification criteria, there were 12 cases of type 1, 17 cases of type 2, 37 cases of type 3, and 21 cases of type 4. The operation time, length of hospital stay, and complications were recorded. The visual analogue scale (VAS) score was used to assess the degree of low back and leg pain before operaion and at 3 days, 3 months, 6 months, and 12 months after operation. The Oswestry disability index (ODI) was used to evaluate the lumbar spine function. At last follow-up, the modified MacNab criteria was used to evaluate the effectiveness. According to the preoperative migrated intervertebral disc classification, the patients were allocated into groups Ⅰ to Ⅳ. The differences in VAS score and ODI were compared., Results: All 87 patients successfully completed the operations. There was no nerve root injury, dural sac injury, or dural tear during operation. The operation time was (58.6±14.6) minutes and the length of hospital stay was (4.0±0.8) days. All incisions healed by first intention after operation. No symptomatic epidural hematoma occurred. All patients were followed up for 12 months. There were significant differences in VAS scores and ODI at each time point after operation when compared with those before operation ( P <0.05). There were significant differences in VAS score at 3 days after operation when compared with that at 3, 6, and 12 months after operation ( P <0.05). For ODI, except that there was no significant difference between 6 and 12 months after operation ( P >0.05), there were significant differences between other time points after operation ( P <0.05). At last follow-up, the effectiveness was rated as excellent in 66 cases, good in 13 cases, and fair in 8 cases according to the modified MacNab criteria, and the excellent and good rate was 90.8%. There was no intervertebral disc herniation recurred during follow-up period. There was no significant difference in VAS score and ODI among groups Ⅰ -Ⅳ before operation and at each time point after operation ( P >0.05)., Conclusion: The UBE technique is safe and effective in the treatment of migrated lumbar intervertebral disc herniation, with a low complication rate and satisfactory early effectiveness.

Objective: Applying for the activity of enzyme in vitro,the research optimized the best preparation procedure for the anticoagulated blood region from Lumbricus., Method: All through our experiment, the content of protein and theactivity of enzyme were examined. The extraction process, the refining technology, concentration processes of Lumbricus were optimized with single factor checking and orthogonal design method., Result: At 37 degrees C, the coarse powder of Lumbricus soaking with 15 fold of 0.9% sodium chloride and ultrasonic extracting 40 minites for three times was the best ultrasonic extraction. Utrafiltration membrane with molecular weights of 30 x 10(3) for refining and 10 x 10(3) for concentrating were selected., Conclusion: Ultrasonic extraction and membrane separation technology, to well improve the effect of purification for the anticoagulant site of Lumbricus, is conducive to further study.

Objective: To investigate the effect of removing bacterial endotoxin in the key processes of Reduning injection., Method: The content of bacterial endotoxins was detected by kenitic-turbidimetry and the removal efficacy was studied before and after using 0.8% of activated carbon and ultrafiltration with molecular weight cut-off of 10 x 10(3)., Result: The adsorption rate of bacterial endotoxins was 78.7% by using activated carbon, while the removal efficacy of bacterial endotoxins was 99.6% with ultrafiltration membrane at cut-off molecular weight 10 x 10(3)., Conclusion: The key technology can effectively guarantee the safety of Reduning injection.

Objective: To investigate the compatible stability of Reduning injection in different dosages with 5% glucose injection and 0.9% sodium chloride injection., Method: The changes before and after compatible application of Reduning injection with 5% glucose injection and 0.9% sodium chloride injection (5: 250, 10: 250, 20: 250, 30: 250, 40: 250, 50: 250, 62. 5: 250) on color, clarity, pH, main ingredients and atlas of HPLC were determined., Result: There were no difference in the color, clarity, pH, the main ingredients and liquid fingerprints of the mixture solutions, but the insoluble particles in them showed significant difference within 6 h., Conclusion: Reduning injection is compatible in different concentrations with 5% glucose injection or 0.9% sodium chloride injection within 4 h, in clinic, it should be used within 2 h, the volume of Reduning injection with 5% glucose injection or 0.9% sodium chloride injection should be no more than 50 mL.

Objective: To investigate the mutations of phenylalanine hydroxylase (PAH) gene in the cases of classical phenylketonuria (PKU)., Methods: The exons 3-12 of the PAH gene in 32 PKU patients from Inner Mongolia were studied by using PCR-single strand conformation polymorphism technique and DNA direct sequencing., Results: Fourteen point mutations were identified. The frequencies of mutations were R243Q (12/64), Y356X (6/64), Y204C (5/64), R261Q (2/64), Y161S (2/64), R252Q (1/64), R111X (2/64), D282G (1/64), S303P (1/64), G239D (1/64), R413P (1/64), IVS7nt+2 (2/64), IVS4nt+3 (1/64) and IVS9nt+34 (2/64). Two novel mutations IVS4nt+3 (G>C) and IVS9nt+34 (G>A) were first found. The S303P (T>C) and D282G (A>G) were first documented in Chinese PAH gene., Conclusion: This study demonstrated the variety of the mutation type PAH gene of PKU in Inner Mongolia population, and confirmed that R243Q, Y356X, Y204C were the hot spots of PAH gene mutation.

Objective: This study was conducted to observe dynamically the serum and stool level of IL-2, IL-6 and IFN-alpha with RV enteritis, and to analyse the relation of them and their relation to clinical manifestations., Methods: The double-antibody sandwich ELISA technique was applied., Results: The positive rate of RV in 119 enteritis cases was 63.0% and the peak month was November. These cytokines had different peak phases in the course of disease. The earliest cytokine which had peak phase was IFN-alpha, the last ones were serum and stool IL-2. There was a negative relation between stool IL-2 and IL-6 (P<0.01). There were positive relation between IL-2 and IFN-alpha, stool IL-6 and IFN-alpha, serum IL-2 and IL-6, stool IL-2 and the recovery time of the enteritis (P<0.01 or P<0.05). Serum and stool level of IL-2 and IFN-alpha had positive relation to the number of lymphocyte plus mononuclear cells (P<0.01 or P<0.05)., Conclusions: IL-2, IL-6 and IFN-alpha play important roles of immunoregulation in RV enteritis, and have close relation to clinical manifestations.