1. [Preliminary study on molecular detection of polysaccharide from Amusium pleuronectes and its intervention to hepatic fibrosis in rats infected with Schistosoma japonicum ].
- Author
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Ye-Chao L, Xiao-Niu T, Wei H, Yu-Xin J, Xiao-Dong Z, Wei G, Qi-Shan S, Guo-Dong W, and Shu-Lin Z
- Subjects
- Animals, Anthelmintics chemistry, Anthelmintics isolation & purification, Anthelmintics therapeutic use, Female, Mice, Mice, Inbred BALB C, Praziquantel therapeutic use, Random Allocation, Rats, Bivalvia chemistry, Liver Cirrhosis drug therapy, Polysaccharides chemistry, Polysaccharides isolation & purification, Polysaccharides therapeutic use, Schistosoma japonicum, Schistosomiasis japonica
- Abstract
Objective: To detect the molecular characterization of polysaccharide purified from Amusium pleuronectes , so as to investigate its role of intervention to the formation of hepatic fibrosis caused by Schistosoma japonicum infection., Methods: The crude polysaccharide from A. pleuronectes was extracted and further purified, and the molecular weight and monosaccharide composition were determined by the high pressure size exclusion chromatography and PMP pre-column derivatization method, respectively. A total of 50 female BALB/c mice were randomly divided into five groups:A (normal group), B (experimental group), C (polysaccharide group), D (praziquantel), and E (polysaccharide + praziquantel group). The mice in B, C, D, or E groups were attacked on the abdominal skin by using the cercariae of S. japonicum (30 ± 2 for each mouse) respectively. After 8 weeks, the mice in C, D, and E groups were administrated by polysaccharide and/or praziquantel, and the mice in B group were instead of saline. All the livers and sera were collected after 16 weeks. HE staining was employed for the livers, and serum IFN-γ and IL-13 were measured by using ELISA kits., Results: The molecular weight of purified polysaccharide from A. pleuronectes was 11.7 kDa. Compared with A and B groups, the serum levels of IFN-γ in C, D, and E groups were significantly increased ( F = 63.525, P < 0.01). However, the serum levels of IL-13 in C, D, and E groups were significantly decreased ( F = 99.788, P < 0.01) compared with that in B group. HE staining showed that the egg nodules and hepatic fibrosis were observed in B, C, D, and E groups. The number of egg nodules and fibrosis degree in E group were milder than those in B group (χ
2 = 7.875, P < 0.05)., Conclusions: The polysaccharide from A. pleuronectes has an obvious effect in preventing hepatic fibrosis process induced by S. japonicum infection, particularly combining with the administration of praziquantel.- Published
- 2018
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