1. [Construction of a novel gene therapy lentiviral vector for drug resistant selection and detection in vivo].
- Author
-
Xu C, Gu Y, Hou W, Cheng T, Zhang T, Que Y, Gao S, Zhang J, Han J, and Xia N
- Subjects
- DNA Modification Methylases genetics, DNA Repair Enzymes genetics, Drug Resistance genetics, Humans, Lentivirus metabolism, Luciferases genetics, Tumor Suppressor Proteins genetics, DNA Modification Methylases biosynthesis, DNA Repair Enzymes biosynthesis, Genetic Therapy methods, Genetic Vectors genetics, Lentivirus genetics, Luciferases biosynthesis, Tumor Suppressor Proteins biosynthesis
- Abstract
Lentiviral vectors were powerful gene delivery tools for gene therapy. We developed a new lentiviral vector pBobi-MIL that constitutively expressed O6-methylguanine-DNAmethyltransferase (MGMT) and Luciferase, linked by the internal ribosomal entry site (IRES), to realize drug tolerance and real time monitoring in vivo. All results from RT-PCR, drug treating clones forming, immunofluorometric assay and chemiluminescence detection showed that cells infected by recombinant lentivirus L-MIL simultaneously expressed these two genes. This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.
- Published
- 2008