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Objective: To establish a UPLC fingerprint method of Xiasangju Granules., Methods: UPLC analysis was performed on a Waters ACQUITY UPLC H-Class system and carried out at 30 °C on a Waters Column ACQUITY UPLC BEH C18 (2.1 mm x 50 mm, 1.7 μm). A binary gradient elution system was composed of acetonitrile (phase A)and 0.5% acetic acid solution (phase B). Detection was performed at the wavelength of 320 nm,the mobile flow rate was at 0.4 mL/min. A matrix including 16 variations (characteristic peaks area)and 12 samples was constructed for similarity evaluation, cluster analysis and principle component analysis., Results: The results showed that the collected samples had a good similarity. A specificity fingerprint was produced and 16 characteristic peaks were designated. 12 samples were divided into 6 groups., Conclusion: It is a reliable, available and quick method for quality control of Xiasangju Granules.

Objective: To establish HPLC fingerprint of Prunella vulgaris and provide evidence for quality control and identification of the Chinese crude drug., Methods: The HPLC method was used, chromatography conditions were Agilent Eclipse XDB-C18 (4.6 mm x 250 mm, 5 microm) column with gradient mobile phase of acetonitrile-0.1% acetic acid, UV detection wavelength was 290 nm and the column temperature was 30 degrees C with the flow rate of 1.0 mL/min, the sample injection was 10 microL., Results: HPLC fingerprints of 19 samples of Prunella vulgaris were established. 14 common peaks were selected as the fingerprint peaks in all samples. Among the obtained fingerprints, most of the detected peaks were separated effectively. 19 samples had high similarities., Conclusion: The established HPLC fingerprint has desirable accuracy, repeatability and stability, which can be used for one of the quality control methods of Prunella vulgaris.

Objectives: To study the mechanism of Yanlieping Formula in treating chronic nonbacterial prostatitis., Methods: Thirty-two C57BL/6 mice were divided into Chinese Traditional Medicine group(Yanlieping group, 10 mice), treatment control group(Cernilton group, 10 mice), model group(6 mice) and normal group(6 mice). The animal model was created by using immunologic adjuvant, and Yanlieping (0.84 g per mouse), Cernilton (7.5 mg per mouse), distilled water(1.05 ml per mouse) and distilled water (0.5 ml per mouse) were respectively administered to the four groups every day for one month. The prostate weight, pathological changes, TNF-alpha and IL-2 in serum were observed., Results: The prostate weight in Yanlieping group and Cernilton group became significantly lower than in the model group(P < 0.05). Pathologic sign of chronic inflammation became better significantly(Yanlieping group showed more significant improvement). The expression of IL-2 in Yanlieping group and Cernilton group were downregulated significantly. And the expression of TNF-alpha in Yianlieping group was higher than that of the model group and the normal group (P < 0.05)., Conclusions: The mechanism of Yanlieping Formula in treating chronic prostatitis may lie in the max urethral close pressure reduction, anti-inflammation, local blood circulation improvement.