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The concept of valve-column module used to create the multi-dimensional gas chromatography was proposed on the basis of investigation of the references of refinery gas analysis and the modern chromatography technique. At first, six basic valve-column modules applied for complex gas analysis were described in detail. Seven channels used to analyze the specific components of refinery gas were listed, which were composed of one or more of the aforementioned valve-column modules. Eighteen kinds of multi-dimensional gas chromatography were induced with parts of those channels. And they were sorted into four columns, five columns, six columns and seven columns according to the number of columns. The advantages and disadvantages and the exchange law were discussed in more details. It was found that two six-way valves and one ten-way valve were equal entirely in valve-column module. The valve-column module had to be designed firstly, and then the analysis channel was built for creation of multi-dimensional gas chromatography system. It was only related to the number of columns, no direct relationship with the number of valves for completing the same analysis task. [ABSTRACT FROM AUTHOR]

Objective: To explore the role of NADPH oxidase 4( NOX4) in transforming growth factor-β( TGF-β)-induced A549 cells migration., Methods: The A549 cells were allocated into five groups: TGF-β( stimulation) group, Normal control group, DPI( NOX4 inhibitor) group, TGF-β + DPI group, and DMSO( solvent control)group. The level of ROS were detected by flow cytometry instrument. The level of NOX4, snail and E-cadherin protein were detected by western blot. Use scratches experiment to express the change of A549 cells migration., Results: After the quantification by Quantity One software, the expression of NOX4 in TGF-β group is( 1. 80 ± 0. 07), the TGF-β +DPI group is( 0. 49 ± 0. 03)( F = 327. 071, P < 0. 001). The change of EMT related protein: the expression of snail protein in TGF-β group is( 9. 0 ± 0. 6), the TGF-β + DPI group is( 1. 8 ± 0. 3)( F = 119. 097, P < 0. 001), the expression of E-cadherin protein in TGF-β group is( 0. 5 ± 0. 1), the TGF-β + DPI group is( 3. 3 ± 0. 3)( F = 71. 063, P <0. 001). These aboveresult indicate that DPI can inhibit the expression of NOX4 and EMTprogress in A549 cells. Then TGF-β + DPI group compared with the TGF-β group, the scratch healing rate is decreased( F = 33. 899, P < 0. 001). It illustrates that DPI can inhibit migration ability of A549 cells., Conclusion: After the NOX4 was inhibited by DPI, TGF-β-induced migration of A549 cells was inhibited. And this phenomenon is associated with the progress of TGF-β-induced EMT.