1. [Effects of RhoA gene silencing by RNA interference on invasion of tongue carcinoma].
- Author
-
Chen Z, Tang YP, Tong L, Wang Y, Zhou Y, Wang Q, Han J, He Z, Liao Y, Fan B, Zou RH, Zhang J, Sun X, and Yan G
- Subjects
- Carcinoma, Squamous Cell genetics, Carcinoma, Squamous Cell metabolism, Down-Regulation, Galectin 3 metabolism, Gene Silencing, Humans, Matrix Metalloproteinase 9 metabolism, RNA, Messenger metabolism, RNA, Small Interfering genetics, Tongue Neoplasms genetics, Tongue Neoplasms metabolism, Transfection, Carcinoma, Squamous Cell pathology, Cell Line, Tumor, RNA Interference, Tongue Neoplasms pathology
- Abstract
Objective: To study the effects of RhoA down-regulation by RNA interference on the invasion of tongue carcinoma Tca8113 and SCC-4., Methods: Determination of the human RhoA sequence as well as the design and constructionof a short specific small interfering RNAs (siRNA) were performed. The siRNA of RhoA gene was transfected into humantongue squamous cell carcinoma Tca8113 and SCC-4 cells line by Lipofectamine 2000. Quantitative real-time polymerasechain reaction was used to examine the mRNA expressionlevels of RhoA. Protein expressions of mRNA, galectin-3,and matrix metalloproteinase (MMP)-9 were evaluated byWestern blot. Transwell invasion assay was performed toassess the invasion ability of tongue carcinoma., Results: RhoA expressions in Tca8113 and SCC-4 cells were reducedsignificantly after transfection of RhoA-siRNA. Protein levels f galectin-3 and MVP-9 were also down-regulated significantly. Invasion ability was inhibited as well., Conclusion: RhoA-siRNA can effectively inhibit RhoA expression in Tca8113 and SCC-4 cells. The invasion ability of tongue carcinoma cells decreased with down-regulation of the protein expressions of galectin-3 and MMP-9, indicating that RhoA-siRNA can inhibit invasion of tongue carcinoma. Results show that RhoA may play an important role in the processes of invasion and metastasis of tongue carcinoma.
- Published
- 2016