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Arsenic exposure-induced cognitive disorders have attracted much attention from scientists. This study employed RNA sequencing to investigate the potential molecular mechanisms underlying neurobehavioral alterations in mice due to arsenic exposure at a transcriptomic level. Male C57BL/6 mice were divided into four groups, the control group (group C), low-dose group (group L), medium-dose group (group M) and high-dose group (group H), which drank deionized water containing 0, 0.15, 1.5 and 15 mg·L-1 AS2O3 respectively for 12 weeks. Cognitive function of the mice was analyzed by the novel object recognition test. RNA sequencing followed by GO enrichment analysis and KEGG pathway enrichment analysis identified differentially expressed genes (DEGs) between control and high dose groups. qRT-PCR was conducted to verify the gene expression levels of target genes. A protein-protein interaction network (PPI) was constructed to identify hub genes from the gene module with the highest connection degree. The findings indicated a significant decrease in both exploration and discrimination indices for arsenic-exposed groups compared to controls. There were 181 DEGs in the high-dose group compared with the control. A total of 217 significantly enriched GO terms were obtained including behavior, regulation of multicellular biological processes, learning and memory, cognition, etc. KEGG enrichment analysis showed that 128 DEGs were enriched in 38 pathways, including cholinergic synapses, dopamine synapses, neurotrophic factor signaling pathways, etc. 7 hub genes were screened from the gene module with the highest connection degree, which were fos, tac1, gad2, drd2, ppp1r1b, chat and drd1a. The results of gene expression levels from qRT-PCR are in good agreement with those from RNA-seq, reflecting the reliability of RNA-seq. It was observed that arsenic exposure led to a significant reduction in mice' s cognitive abilities. The identified hub genes and key pathways from this study offer valuable insights into exploring the mechanisms underlying neurobehavioral changes in mice due to arsenic exposure. [ABSTRACT FROM AUTHOR]

Objective: To review the research progress of indication and treatment of graft in shoulder superior capsular reconstruction (SCR) for rotator cuff tear (RCT)., Methods: The literature related to shoulder SCR in recent years was extensively reviewed, and the anatomy, biomechanics, surgical indications, and treatment of graft in SCR were summarized., Results: Superior capsule plays a role as a functional complex with rotator cuff, ligament, and whole capsule. SCR can effectively restore the superior stability of the shoulder. The indications of SCR include the irreparable massive RCT, massive RCT combined with pseudoparalysis shoulder, medium/large RCT with severe degenerative rotator cuff tissue, and dual-layer RCT. In order to achieve a better healing of tendon-bone in graft and decrease the rate of long-term graft retearing, it is essential to select an appropriate thickness graft, fix the graft in right intensity, and get a better capsular continuity., Conclusion: The technique of SCR advanced to SCR for reinforcement and it is indicated from substantial massive RCT to severe degeneration of rotator cuff tissue. Graft treatment is the key step for a successful SCR.

Objective: To evaluate the short-term effectiveness of arthroscopically capsular vertical mattress suturing for shoulder recurrent anterior dislocation combined with joint laxity., Methods: A retrospective analysis was performed on 6 shoulder recurrent anterior dislocation patients combined with joint laxity treated with arthroscopically capsular vertical mattress suturing between January 2017 and December 2018. There were 5 males and 1 female with an average age of 20.8 years (range, 19-24 years). The number of shoulder dislocation was 3-18 times, with an average of 9.5 times. The disease duration ranged from 2 to 60 months, with an average of 25.3 months. The preoperative Beighton score was 4-7, with an average of 5.8; the Instability Severity Index Score (ISIS) was 2-5, with an average of 3.5. There were 5 cases of simple Bankart injury and 1 case of bony Bankart injury. The range of motion of shoulder joint (including active flexion and lifting, external rotation, abduction and external rotation, and internal ratation) was recorded before operation and at last follow-up; Oxford shoulder instability score, Rowe shoulder instability score, and Simple Shoulder Test (SST) score were used to evaluate shoulder joint function before operation, at 6 months after operation, and at last follow-up, and complications were recorded., Results: All patients were followed up 16-28 months (mean, 19.3 months). During the follow-up, all patients had satisfactory motor function, and no re-dislocation and postoperative neurovascular complications occurred. At last follow-up, the activities of active external rotation and abduction and external rotation were significantly improved when compared with those before operation ( P <0.05); the activities of active flexion and lifting and internal rotation were not limited before and after operation, and the difference was not significant ( P >0.05). The Oxford shoulder instability score, Rowe shoulder instability score, and SST score at 6 months after operation and at last follow-up were significantly improved when compared with those before operation ( P <0.05); there was no significant difference between at 6 months after operation and at last follow-up ( P >0.05)., Conclusion: The treatment of shoulder recurrent anterior dislocation combined with joint laxity by arthroscopically vertical matress suturing can achieve good short-term effectiveness.

Objective: To detect the differentially expressed circular RNA (circRNA) in rotator cuff tendinopathy and analyze the potential molecular mechanism of these parental genes., Methods: Ten supraspinatus tendons donated from patients who underwent tendon repair surgery between June 2018 and June 2019 were used for RNA-sequence. All rotator cuff tendinopathy and normal tendon samples were confirmed by MRI, histological staining, and observation by arthroscopy. All pathological tendons were matched with tendon samples for patients' age, gender, body mass index, and Bonar score. The bioinformatic analysis was performed based on the differentially expressed circRNA and their parental genes, including gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and competing endogenous RNA (ceRNA) network construction., Results: There were 94 differentially expressed circRNAs, including 31 up-regulated and 63 down-regulated, detected between the rotator cuff tendinopathy and normal tendon samples with |log2 fold change (FC)| >2, P <0.05. GO analysis showed that the genes were mostly enriched in response to cyclic adenosine monophosphate (cAMP). KEGG pathway analysis showed that the most genes were enriched in extracellular matrix-receptor interaction, protein digestion and absorption, cell cycle, and nuclear factor κB signaling pathway. ceRNA networks showed the interactions among circRNAs, mRNAs, and miRNAs. And circRNA.8951-has-miR-6089-DNMT3B was the most sum max energy., Conclusion: This bioinformatic study reveals several potential therapeutic targets for rotator cuff tendinopathy, which paves the way to better treatment and prevention of this disorder.

Objective: To evaluate the mid-term effectiveness of arthrolysis and hinged external fixation for the treatment of stiff elbow caused by heterotrophic ossification., Methods: Between January 2014 and December 2017, 11 patients with stiff elbow caused by heterotrophic ossification were admitted. There were 9 males and 2 females with an average age of 32 years (range, 14-48 years), and left side in 6 cases and right side in 5 cases. The cause of stiff elbow included humerus fracture in 5 cases, ulna fracture in 2 cases, fracture of capitulum radii in 1 case, dislocation of capitulum radii in 1 case, terrible triad of the elbow in 1 case, and soft tissue injury in 1 case. The disease duration ranged from 7 to 18 months (mean, 11 months). Preoperative active range of motion of elbow was (19.6±17.5)° and Mayo score was 34.1±9.7. All patients received the treatment of arthrolysis and debridement of heterotrophic ossification lesion combining hinged external fixator, and active and passive rehabilitation with the help of hinged external fixator. The hinged external fixators were removed after 2 months., Results: All patients were followed up 13-36 months (mean, 19.1 months). All incisions healed by first intention, and no complication of infection or nerve lesion occurred postoperatively. At last follow-up, the results of X-ray films showed that no heterotrophic ossification recurred. The active range of motion of elbow was (116.4±16.6)° and Mayo score was 93.2±7.8, showing significant differences when compared with preoperative ones ( t =17.508, P =0.000; t =16.618, P =0.000)., Conclusion: The application of arthrolysis and debridement of heterotrophic ossification lesion combining hinged external fixator can improve the elbow's range of motion significantly and obtain a good mid-term effectiveness.

Objective: To investigate the effectiveness of tibio-talo-calcaneal arthrodesis with full thread headless compression screws via combined minimal incisions., Methods: Between January 2012 and December 2016, 36 patients (36 feet) with ankle diseases underwent tibio-talo-calcaneal arthrodesis with parallel double thread headless compression screws via minimal anterior and lateral oblique incisions. There were 14 males and 22 females with an average age of 53.8 years (range, 18-76 years). There were 19 cases of left feet and 17 cases of right feet. There were 21 cases of talar necrosis, 7 cases of post-traumatic arthritis, 3 cases of rheumatoid arthritis, 2 cases of tuberculosis infection (inactive), 1 case of talar absence, 1 case of Charcot’s disease, and 1 case of pigmented villonodular synovitis of ankle and subtalar joints. Preoperative American Orthopaedic Foot and Ankle Society (AOFAS) score and visual analogue scale (VAS) score were 53.7±2.5 and 5.9±0.2, respectively. The operation time was recorded and the wound healing and complications were observed. The bone healing was assessed by X-ray film and CT scanning. The function and pain of joint were evaluated by AOFAS and VAS scores., Results: The mean operation time was 49.8 minutes (range, 33-82 minutes). Incision infection occurred in 1 patient (2.8%) at 3 weeks after operation, and recovered after debridement. The other incisions healed by first intention without complications. Thirty-five patients were followed up with an average of 18.5 months (range, 12-29 months). Imaging examination showed fusion of the ankle and subtalar joints with an average fusion time of 10.9 weeks (range, 8-15 weeks). After 1 year, the AOFAS score (84.7±0.6) and VAS score (0.3±0.1) were significantly higher than preoperative scores ( t =12.596, P =0.000; t =30.393, P =0.000)., Conclusion: It is an effective surgical method of tibio-talo-calcaneal arthrodesis with full thread headless compression screws via combined minimal incisions for end-stage ankle disease because of the less complications incidence and the higher postoperative fusion rate.

Objective: To explore the effect of different intensity treadmill training on the repair of micro-injured Achilles tendon induced by collagenase in rats., Methods: Seventy-two 8-week-old male Sprague Dawley rats (weighing, 200-250 g) were selected. After adaptive treadmill training for 1 week, rats were injected with 30 μL type I collagenase solution (10 mg/mL) into both Achilles tendons to make micro-injured Achilles tendon models. After 1 week of cage feeding, the rats were randomly divided into 3 groups: the control group, the low-intensity group, and the high-intensity group, 24 rats each group. The rats in control group could move freely, and the rats underwent daily treadmill training at the intensity of 13 m/min and 20 min/d in the low-intensity group and at the intensity of 17 m/min and 60 min/d in the high-intensity group. At immediate, 1 week, and 4 weeks after training, bilateral Achilles tendons were collected from 8 rats of each group for gross observation, histological analysis, and mechanical testing., Results: At immediate after training, there was no significant difference in the gross observation, histological observation, and biomechanical properties of the Achilles tendon between groups ( P >0.05). The gross observation showed connective tissue hyperplasia near Achilles tendon and lackluster tendon in each group at 1 week; hyperplasia significantly reduced in the low-intensity group when compared with the control group, and there were more connective tissue and a large number of neovascularization in the high-intensity group at 4 weeks. At 1 week, there was no significant difference in the semi-quantitative histological total score between groups ( P >0.05), but there were significant differences in vascularity between low-intensity group or high-intensity group and control group ( P <0.05). At 4 weeks, the semi-quantitative histological total score was significantly higher in high-intensity group than control group and low-intensity group ( P <0.05), and in control group than low-intensity group ( P <0.05). There were significant differences in collagen arrangement, cell morphology, abnormal cells, and vascularity between low-intensity group and high-intensity group or control group ( P <0.05). And there was significant difference in abnormal cells between high-intensity group and control group ( P <0.05). The mechanical testing showed that there was no significant difference in cross-sectional area of the Achilles tendon, the ultimate force, tensile strength, and elastic modulus between groups at 1 week ( P >0.05); the low-intensity group was significantly higher than the control group in the ultimate force and the tensile strength ( P <0.05), and than high-intensity group in the ultimate force and elastic modulus ( P <0.05), but no significant difference was found in the other indexes between groups ( P >0.05) at 4 weeks., Conclusion: Low-intensity treadmill training can promote the repair of rat micro-injured Achilles tendon induced by collagenase.

Objective: To explore the situation of HIV infection among currently married MSM and the features of related sexual behavior on both males and females in Gansu province., Methods: By "Place the recruitment" methodology, 297 currently married MSM were recruited in Lanzhou and Tianshui cities of Gansu province. Participants filled in a questionnaire and underwent HIV antibody testing. SPSS 17.0 software was used to analyze the result and differences between positive HIV MSM and negative HIV MSM. Possible factors that influencing the HIV infection among currently married MSM were analyzed., Results: Among those currently married MSM, the HIV positive rate was 4.7%. 94.6% participants admitted having had anal sex with male partners in the past 6 months while 30.9% participants had more than 2 male sex partners in the past week, with the rate of condoms use as 45.9%. 69.0% of the participants had sex with females in the past 6 months, with the rate of condoms use as 24.9%. 59.0% of the participants did not use condoms when having sexual relations with partners. Among HIV positive married MSM, the rate of AIDS awareness was 64.3% and 7.1% of them ever having had HIV testing last year and 92.9% of them had anal sex with males. In the recent 6 months, 14.3% had ever used condom each time when having anal sex with males. 35.7% of the cases ever having had sex with females, with the rate of condom use as 60.0%., Conclusion: HIV prevalence, low AIDS awareness, low HIV rate of testing and high rate of unprotected sex behavior with either sex partners were found among currently married MSM in Gansu province., Results: of this study called for behavioral intervention and HIV testing measures should be taken immediately.