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In order to understand the ecosystem health degree of the lake area groups on the west bank of Wangyu River, the structural equation model was constructed to evaluate the health of the River, and the suitability of the index system and evaluation method was verified as well. This paper analyzes the rationality of the evaluation results, classifies the health levels of water ecosystems in different regions, determines the key indicators affecting the health of lake water ecosystems, expounds the health status of lake area groups in the research area, and puts forward reasonable suggestions on the existing problems. The results show that total phosphorus and ammonia nitrogen are the two most direct factors of water pollution. Total nitrogen factor load of sediment is large, which is the main endogenous pollution load of the Lake. Per capita GDP and gross industrial product have serious impacts on ecosystems and cause pollution to lakes. According to the results of the model, the lake eco-system health was divided into five grades, and 57.8% of the sampled sections in the study area were found to be unhealthy or diseased, more than half of which indicated that the water ecosystem of lake groups damaged and needs to be restored. [ABSTRACT FROM AUTHOR]

Objective: To detect the expression of miR-221/222 in serum and plasma cells in patients with monoclonal gammopathy of undetermined significance(MGUS) and multiple myeloma(MM), and to explore the possibility of miR-221/222 as biomarkers in the diagnosis and prognosis predicting of MGUS and MM. Methods: Bone marrow and serum samples from 14 patients with newly diagnosed MGUS, 81 patients with newly diagnosed or relapsed MM and 10 controls were collected from Sir Run Run Shaw Hospital of Zhejiang University and Tongde Hospital of Zhejiang Province during January 2013 and December 2015. The expressions of miR-221/222 in serum and in sorted CD138 positive plasma cells were detected by qRT-PCR, and the relative expression of miR-221/222 (Δct) was compared between the groups. Serum levels of miR-221 before and after treatment were compared in both remission group ( n =22) and refractory group ( n =13) in MM patients, and its correlation with serum level of β 2 -MG was assessed using Pearson's correlation analysis. Results: Serum levels of miR-221/222 in MGUS and MM groups were significantly higher than those in control group (all P <0.01), while miR-221/222 levels in plasma cells were significantly lower in MGUS and MM groups than those in the control group ( P <0.05 or<0.01). No significant difference in miR-221/222 levels in serum and plasma cells was observed between MGUS group and MM group (all P >0.05). There was no correlation between miR-221/222 levels in serum and plasma cells ( r =0.024 and -0.127, all P >0.05), but miR-221 levels were correlated with miR-222 levels in both serum and plasma cells ( r =0.534 and 0.552, all P <0.01). Receiver operating characteristic (ROC) curves showed that the areas under the curve (AUCs) of serum miR-221/222, plasma cell miR-221/222 in diagnosis of MGUS/MM were 0.968, 0.976, 0.801 and 0.727, respectively. There was no significant difference in serum level of miR-221 among MM patients with different paraprotein isotypes ( P >0.05), but serum level of miR-221 in patients with relapsed MM was higher than that in patients with newly diagnosed MM ( P <0.01). Compared with the patients with MGUS or MM stageⅠ and Ⅱ, patients with MM stage Ⅲ were of higher serum levels of miR-221 ( P <0.01). Serum level of miR-221 decreased after chemotherapy in the remission group ( U =51.5, P <0.01), but such decrease was not observed in the refractory group ( U =67.5, P >0.05). Serum level of β 2 -MG was positively correlated with serum level of miR-221 ( r =0.524, P <0.01). Conclusion: miR-221/222 in serum and plasma cells may be biomarkers for early diagnosis of MGUS, and are helpful for diagnosis and efficacy evaluation of MM.