1. The effect of NF-κB signaling pathway regulation on PD-L1 expression of Enterococcus faecalis LTA-stimulated macrophages
- Author
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YANG Yanling, LIU Runze, SUN Qing, FAN Wei
- Subjects
enterococcus faecalis ,lipoteichoic acid ,immune checkpoint ,nf-κb ,Dentistry ,RK1-715 ,Other systems of medicine ,RZ201-999 - Abstract
Objective To investigate the effect of Enterococcus faecalis (E. faecalis) lipoteichoic acid (LTA) on the expression of the macrophage immune checkpoint-programmed cell death protein ligand 1 (PD-L1) and explore the possible regulatory mechanisms. Methods In vitro model was established by stimulating mouse monocyte macrophage cell line (RAW264.7) with different concentrations of E. faecalis LTA. PD-L1 expression and nuclear factor kappa-B (NF-κB) p65 phosphorylation level were detected using flow cytometry, quantitative real-time polymerase chain reaction (qRT-PCR), Western blot and cellular immunofluorescence. Macrophages were pretreated with the NF-κB signaling pathway inhibitor BAY 11-7082, then the expression of PD-L1 and p65 phosphorylation level were detected by the above methods. Results LTA induced PD-L1 expression in RAW264.7 cells, and p65 phosphorylation level increased (P
- Published
- 2024
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