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The set of guidelines for the diagnosis and treatment of thyroid nodules and differentiated thyroid cancer (the second edition) was published in 2023 in China. Based on the first (2012) edition, the current set was revised jointly by nearly 100 experts in endocrinology, thyroid surgery, oncology, nuclear medicine, ultrasound medicine, and pathology from seven national societies for one year. The new version of the guideline is still divided into two parts, namely, thyroid nodules and differentiated thyroid cancer. The writing mode of asking clinical questions, explaining and giving recommendations is adopted, and a total of 117 recommendations are provided. This article aims to compare the variations in the differentiation of benign and malignant thyroid nodules and surgical treatment of differentiated thyroid cancer between the new and old versions from the perspective of surgery. The author's own understanding and experiences are also discussed.

Oculomotor nerve palsy is a nerve condition that affects patient’s vision and quality of life. This article summarizes the nursing experience of a patient with mild oculomotor nerve paralysis treated with plum-blossom needle tapping therapy. Based on the differentiation of symptoms and signs in Traditional Chinese Medicine, plum-blossom needle technique therapy, as well as daily life support, dietary care, medication, and emotional nursing interventions, can effectively improve symptoms such as ptosis, strabismus, and limited eye movement in the patient. (动眼神经麻痹严重影响患者视觉及生活质量。本文总结采用梅花针叩刺法治疗1例轻度动眼神经麻痹患者的护理经验。根据护理评估及中医辨证分型, 在生活起居、饮食护理、情志护理基础上, 采用梅花针中医护理技术, 有效改善了患者上睑下垂、斜视、眼球活动受限等症状。)

Objective To explore the expression and function of DEP domain containing 1 (DEPDC1) in uterine corpus endometrial cancer(UCEC). Methods The Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases were used to analyze the expression of DEPDC1 mRNA in normal endometrial tissues and UCEC tissues, the relationship with patient clinicopathological parameters and prognosis. Immuno-histochemistry (IHC), Western blot and RT-qPCR were used to detect the expression of DEPDC1 in UCEC cell lines and tissue. The CCK-8 assay, colony formation assay, and tumor xenograft were used to investigate the effect of DEPDC1 on cancer proliferation. Results DEPDC1 mRNA was up-regulated in UCEC (P＜0.001) and was correlated with the clinical stages (P＜0.001), tissue differentiation (P＜0.05) and histological type(P＜0.001) of UCEC. Compared with the DEPDC1 mRNA low expression patients, the patients with high expression of DEPDC1 showed lower disease-specific survival(DSS) and progress-free interval (PFI) (P＜0.01). The IHC, Western blot, and RT-qPCR showed that DEPDC1 was up-regulated both in UCEC tissue and cell lines. Down regulation of DEPDC1 inhibited the colony formation, invasion and tumor sphere formation, cell proliferation rate, and tumourigenesis in vivo. Conclusions DEPDC1 is up-regulated in UCEC that is correlated with the malignant progression and poor prognosis of UCEC.

This study was undertaken in order to improve the stability, water solubility and gastrointestinal release of phytosterols (PS). Zein-PS nanoparticles were prepared by the anti-solvent method. Single factor experiments and orthogonal array design methods were adopted to optimize the preparation conditions based on the stability and encapsulation efficiency of zein-PS nanoparticles. The structure and functional properties of the prepared samples were characterized by using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and simulated gastrointestinal digestion. The results showed that the optimal preparation conditions were determined as follows: zein/PS ratio of 15:1 (m/m), hydration time of 2 h, hydration temperature of 55 ℃, and ultrasonic treatment time of 20 min. The encapsulation efficiency of the nanoparticles prepared using the optimal conditions was 84.97%, particle size (479.76 ± 0.38) nm, and zeta potential (−22.79 ± 0.015) mV, suggesting small particle size and high stability. The nanoparticles with a zein/PS ratio of 15:1 had better re-solubility and water solubility. SEM revealed the formation of a compact network structure on the surface of the nanoparticles and FTIR spectra showed that it changed at 3 313.16 and 1 523.51 cm-1, indicating the presence of hydrogen bonding and electrostatic interaction between zein and PS. Compared with PS, the release rates of the nanoparticles in simulated gastrointestinal fluids were reduced by 49.03% and 28.11%, respectively, showing a good sustained-release effect. In addition, after storage for 30 days, the change in the particle size of the nanoparticles was smaller at 4 ℃ than at 25 ℃, and the encapsulation efficiency remained above 70%. Therefore, zein-PS composite nanoparticles have good stability and sustained release property, which has potential application prospects in the food industry.

In order to prolong the shelf life of milk fan, casein-chitosan edible films incorporated with antimicrobial peptide BCp12 were prepared. One-factor-at-a-time method combined with orthogonal array design was used to determine the optimum preparation process based on elongation at break and tensile strength. The antimicrobial activity of the composite film was evaluated, and its formation mechanism was explored by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and molecular docking. Finally, the composite film was applied to the preservation of milk fan. The results showed that the optimum preparation conditions were casein/chitosan mass ratio 1:1.5, BCp12 concentration 1 mg/mL, glycerin concentration 1.5% (m/m), and drying temperature 55 ℃. SEM showed good compatibility of the composite film and FT-IR analysis revealed that the film exhibited an absorption peak at 1 545.67 cm–1 attributed to the stretching vibration of C–O. Molecular docking showed that the hydrogen bond interaction between BCp12 and casein and chitosan occurred through the active sites of residues Y4 and –NH2. Compared with the control group, the change of peroxide value of milk fan treated with the antimicrobial film was slower and the shelf life was prolonged by 60 days during storage at 4 ℃, indicating the film could inhibit the growth of spoilage bacteria. The results of this study provide a technical reference for the development and application of chitosan-casein composite films incorporated with BCp12.

This paper reviewed the nursing measures based on the idea of "heterozygous treatment" for a patient in the face-down position after vitrectomy. A comprehensive treatment with various methods, including psychological care, position management, complication prevention and Traditional Chinese Medicine nursing techniques, were carried out to improve the postoperative rehabilitation and quality of life of the patient. (本文回顾“杂合以治”思想应用于1例玻璃体切割术后面向下体位患者的护理体会。基于“杂合以治”思想, 采用多种方法给予患者对症综合治疗, 对促进术眼康复和提高患者生活质量有积极作用。)

The major problems of TiO_2 photocatalyst are easy aggregation and deactivation, and obvious difficulty in separation. In order to overcome some of the drawbacks of TiO_2 photocatalyst in suspension, zeolite(NaY) molecular sieves were used to support TiO_2. The preparation conditions of TiO_2/NaY composite photocatalyst were optimized in this research. Moreover, the effect of NaY on crystal type, phase transition temperature and photocatalytic properties was investigated, and the influence of TiO_2/NaY on the degradation of organic waste water was analyzed. The results indicate that the TiO_2/NaY composite photocatalyst exhibits the optimal photocatalytic activity under the conditions of TiO_2 loading of 35%, sintering temperature of 500 °C, roasting time of 2 h and catalyst dosage of 0.08 g(methylene blue solution of 100 mL). When methylene blue solution of an initial concentration of 20 mg/L was treated for 0.5 h with TiO_2/NaY composite photocatalyst, the photocatalytic degradation rate was 95.42%, 22.3% higher than that of TiO_2. High stability, high ultraviolet light transmission and regular microporous and cage structure of NaY is conducive to not only recycle of TiO_2 but also improvement of photocatalytic activity of TiO_2.

This paper summarized the nursing experience of external treatment of Traditional Chinese Medicine (TCM) in treating chalazion in children, including routine care, ear-tip bloodletting, Chinese herbal medicine hot compress, etc. The TCM external treatment, characterized by easy–to-use, obvious efficacy and high patient acceptance, is effect to relieve the inflammatory response caused by chalazion. (本文总结耳尖放血联合中药热敷治疗小儿霰粒肿的相关经验, 包括常规护理、耳尖放血操作方法、中药组方以及热敷方法等。中医外治法操作简单、疗效明显, 且具有较好的依从性, 能够有效缓解霰粒肿炎症反应, 减轻患儿痛苦。)

Research progress and application of stereo-complexed polylactide fibers structure and property were summarized. Crystal structure of stereo-complexed polylactide fiber was introduced. Mechanical, thermal, hydrolysis and dyeing property of stereo-complexed polylactide fiber were analyzed. Applications of stereo-complexed polylactide fiber in fields of textile, medical hygiene, sewage treatment and air filtration were summarized. It is considered that the excellent thermal stability of stereo-complexed polylactide fiber must have higher content and stable stereo-complexed crystals. It is necessary to conduct in-depth basic research on structure and property, crystal structure regulation technology suitable for continuous fiber processing should be developed and researched, industrial application of stereo-complexed polylactide fiber should be promoted. [ABSTRACT FROM AUTHOR]

Objective: The phosphorproteomics technique was used to analyze the differential expression of phosphorylated proteins in the human lung adenocarcinoma A549 cells treated with antimicrobial peptide merecidin, and to explore the effect of merecidin on the protein activity and function of lung adenocarcinoma A549 cells and the signal pathway involved. Methods: A549 cells were treated with 9 μmol/L merecidin for 6 h. The total protein was collected and extracted, and SDS-PAGE experiment was used to test the total protein extraction efficiency. Pancreatin was added to digest the protein. The peptides obtained by enzymatic hydrolysis were labeled with TMT, fractionated by HPLC, enriching phosphorylated modified peptides by IMAC, and separate peptides by HPLC-MS/MS (liquid chromatographymass spectrometry) technology. The identified data were screened using the standard of localization probability>0.75, and the phosphoproteomics data were analyzed using GO (Gene Ontology) database, KEGG (Kyoto Encyclopedia of Genes and Genomes) database and STRING database. Results: SDS-PAGE results showed that the total protein separation of A549 cells treated with 9 μmol/L merecidin was clear and no obvious degradation, and there was significant difference in protein bands between the experimental group and the control group. A total of 10 320 phosphorylation modification sites on 3 089 proteins were identified by mass spectrometry, of which 753 proteins and 1 172 phosphorylation sites were screened out with |Fold Change| >1.5 and P<0.05 as the threshold. Protein function enrichment showed that functions of proteins with significant changes in phosphorylation level mainly focused on molecular binding, metabolic activity, molecular function regulation, cell process, biological function and other aspects. Bioinformatics results of integrated pathway showed that differentially expressed proteins were associated with Ras, PI3K/AKT, mTor, AMPK etc. COG database screening showed that the differentially expressed phosphorylated proteins were concentrated in cell signal transduction, processing and modification of RNA transcription and translation, protein synthesis of ribosome, formation of cytoskeleton proteins, intracellular substance transportation, secretion and vesicle transportation etc. At the protein interaction level, after merecidin treatment, an interaction network with MAPK1, RPL23A, SRSF3H, NCBP1, etc. as key proteins was formed in A549 cells; proteins such as ATG2B and ULK1 etc. were significantly up-regulated, while proteins such as MAPK1 and AKT1 etc. were significantly down-regulated. Conclusion: Phosphoproteomic analysis shows that the antimicrobial peptide merecidin may play a role in multiple biological functions and multiple signaling pathways through key proteins such as MAPK1, RPL23A, SRSF3H and AKT1, and promote the apoptosis and autophagy of lung adenocarcinoma A549 cells, thereby inhibiting cell proliferation. [ABSTRACT FROM AUTHOR]

bjective To investigate the turnover intention among junior nurses based on the dual perspectives(junior nurses and hospital managements)of psychological contract. Methods Totally 384 nurses were investigated by the Psychological Contract Scale and Turnover Intention Scale. Results: The score of turnover intention in junior nurses was(3.20±0.75). From the present situation of psychological contract from the perspective of dual perception perspective, organizers had higher scores than the individual perception of junior nurses(P<0.01). The influential factors of turnover intention were the age, job title, labor properties, differences in perception of the organization's normative obligation and the individual's development obligation(P<0.05). Conclusion: Hospital managers should pay more attention to the psychological contract management of juniors nurses, and the cultivation of individual adjustment factors, so as to improve juniors nurses' perception level of psychological contract and reduce their turnover intention. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of different antibacterial agents combined with tigecycline in patients with multidrug-resistant Acinetobacter baumannii (MDRAB)-related ventilator-associated pneumonia (VAP), and to provide options for better treatment of the disease in clinical practice. Methods A retrospective analysis was performed for the clinical data of the patients with MDRAB-related VAP who were admitted from January 2016 to August 2023 in Qingdao municipal Hospital, and according to the treatment regimen, they were divided into tigecycline group (group A) with 56 patients, cefoperazone-sulbactam combination therapy group (B group) with 42 patients, and carbapenem combination therapy group (group C) with 24 patients. The three groups were compared in terms of infection and related outcome measures. Results There were no significant diffe-rences in the type of pathogene infected and infection sites between the three groups (P>0.05). Groups B and C had a significantly higher bacterial clearance rate than group A (χ2=5.38,4.48,P0.05). Conclusion Tigecycline combined with cefoperazone-sulbactam or carbapenem achieves a higher bacterial clearance rate than tigecycline alone in MDRAB-related VAP, and combined medication is a relatively good treatment option.

[Objective] “Shrinking county seats” is a new topic in national and regional governance. As the center of the county, a county seat is not only the center of population distribution, but also the center of comprehensive governance in the county. The shrinkage of the county seat's population has more exemplary and symbolic significance. Resolving the population shrinkage, economic decline, and slow social development problems of county seats has become an important issue in promoting urban-rural integration and achieving rural revitalization. The high-quality development of the Yellow River Basin has become a national strategy, but it is facing dual pressures of population reduction and loss. It is of great practical significance to scientifically identify the types of shrinking county seats, analyze the periodicity and stability of the shrinking process, and promote the high-quality development of the Yellow River Basin. [Method] This study used population shrinkage rate to systematically analyze the process and types of population shrinkage in county seats in the Yellow River Basin from 2000 to 2020. [Results] The study found that: (1) Population shrinkage in the county seats is mainly characterized by temporary and periodic contractions. (2) Shrinking county seats are concentrated in contiguous poverty-stricken areas, mineral resource development zones, ethnic minority settlements, and inter-provincial border areas. (3) The population in the county seats alternated between contraction and stagnation (or expansion), with the cumulative years of contraction mainly within 2 to 4 years, followed by 5 to 8 years, roughly in line with China's unique Five Year Plan periods (Kitchin Cycle) and Juglar Cycle. [Conclusion] The 381 county seats in the Yellow River Basin mainly experienced temporary and periodic contraction, which has not resulted in worrying results. But attention should be paid to the concentrated distribution areas and targeted response strategies should be formulated.

[Purpose/Significance] The research on the influence of think tanks has become a topic of extensive interest and in-depth exploration in the field of think tanks. This paper objectively summarizes and analyzes the current situation and development trend of the research on the influence of think tanks, in order to provide useful reference for the research in this field and promote the development of new types of think tanks with Chinese characteristics. [Method/Process] CiteSpace was used to conduct a visualization analysis of the relevant literature in this field with high influence at home and abroad. The development characteristics and trend of this field were analyzed through the knowledge mapping of authors, research institutions, highly cited articles, keyword co-occurrence and cluster analysis. [Results/Conclusions] it is found that foreign research focuses on the application of think tanks in professional fields, such as political policy, foreign affairs, economy, environment, public health and so on. In contrast, domestic research shows that low degree of cooperation between researchers and institutions is carried out, and the research on the influence of think tanks in professional fields (economy, diplomacy, etc.) is insufficient. At the same time, discovering new media based on time series graph is an important research direction in this field in the future.

Objective: To assess the application value of CNVPLUS-array for the genetic analysis of Spinal muscular atrophy (SMA)., Methods: From June 2021 to December 2022, CNVPLUS-array technique was employed to test the SMN1 and SMN2 genes among peripheral blood samples from 17 suspected SMA patients, 18 core families with suspected SMA, and 25 healthy individuals. The results were compared with those of multiple ligation-dependent probe amplification (MLPA) assay. Samples with inconsistent results were subjected to nested PCR or comprehensive analysis of SMA., Results: CNVPLUS-array has identified 35 SMA patients, 36 carriers, and 25 healthy individuals. In comparison, MLPA has identified 34 SMA patients, 36 carriers, and 26 healthy individuals. The two methods demonstrated a high consistency (Kappa = 0.968, P < 0.001). Additionally, CNVPLUS-array has identified one patient with compound heterozygous variants of SMN1 and one carrier with a [2+0] genotype., Conclusion: CNVPLUS-array not only can accurately determine the copy numbers of SMN1 and SMN2 genes, but also identify point mutations in SMN1 and [2+0] carriers, which has offered a new method for the genetic testing of SMA.

Objectives: This study aims to evaluate the efficacy of concentrated growth factor (CGF) membrane and collagen as barrier materials in sealing the alveolar socket in alveolar ridge preservation (ARP) in the posterior region during a one-year follow-up., Methods: A total of 24 patients who underwent ARP in the posterior region were selected for inclusion and randomly assigned to the CGF group (12 cases) and Collagen group (12 cases). The patients in both groups underwent extraction of posterior teeth. The extraction sockets were filled with a bone substitute to the level of the pre-extraction buccal and lingual or palatal alveolar bone plates. The wounds in the CGF group were closed with a fabricated CGF overlaying the upper edge of the bone substitute material, whereas those in the Collagen group were closed with Bio-Oss Collagen. The implants were placed after 6 months. The evaluation was based on implant retention, re-grafting rate, and vertical and horizontal alveolar ridge bone volume changes measured by cone beam computed tomography (CBCT). Data were statistically analyzed using SPSS 28.0 software., Results: No patient withdrew throughout the follow-up period. No implant failure and no severe peri-implant or mucosal soft tissue complications were observed. Six months after the operation, the degree of vertical alveolar ridge height resorption in the CGF group was lower than that in the Collagen group ( P <0.05). There were no statistically difference between the groups at 1 year after the operation ( P >0.05). The amount of bone reduction in horizontal alveolar ridge width showed no difference between the groups at 6 months and 1 year after surgery ( P >0.05)., Conclusions: CGF membrane and Bio-Oss Collagen as barrier materials for posterior ARP inhibited reduction in alveolar ridge bone mass.

Biomanufacturing uses biological systems, including cells, microorganisms, and enzymes, to produce natural or synthetic molecules with biological activities for use in various industries, such as pharmaceuticals, cosmetics, and agriculture. These bioactive compounds are expected to play important roles in improving the quality of life and prolonging its length. Fortunately, recent advances in synthetic biology and automation technologies have accelerated the development of biomanufacturing, enabling us to create new products and replace conventional methods in a more sustainable manner. As of now, the role of biomanufacturing in the growth and innovation of bioeconomy is steadily increasing, and this techbology becomes a prevalent technology in global markets. To gain a comprehensive understanding of this field, this article presents a retrospective review of Bloomage Biotechnology's Research and Development and briefly reviews the developments of biomanufacturing and offers insights into the futre prospects. In conclusion, biomanufacturing will continue to be an important, environmentally friendly, and sustainable production mode in the ongoing development of bioeconomy.

Background: Tumor recurrence and drug resistance are the main causes of death in tumor patients. The family of acetaldehyde dehydrogenase (ALDH) is closely related to the proliferation, migration, invasion and resistance of tumor cells, and different ALDH subtypes are expressed in different tumor cells. The aim of this study is to elucidate the ALDH subtype in human lung adenocarcinoma HCC-827/GR cells, which resistant to the gefitinib., Methods: The human lung adenocarcinoma HCC-827 cells were used to generate the gefitinib-resistant HCC-827/GR cells; the expression of ALDH subtype in either HCC-827 or HCC-827/GR was detected by flow cytometry; The proliferative capacity and sensitivity to gefitinib of hcc-827/GR cells were analyzed by MTT assay before and after treatment with 100 μmol/L diethyllaminaldehyde (DEAB); Real-time quantitative PCR was used to detect the expression of ALDH subtypes at mRNA levels in hcc-827 cells and hcc-827/GR cells., Results: Compared with HCC-827 cells, the positive rate of ALDH in HCC-827/GR cells increased. The proliferation ability of HCC-827/GR cells decreased after treatment with 100 μmol/L DEAB. Compared with HCC-827 cells, the expression of ALDH1A1 and ALDH1L1 mRNA was increased in hcc-827/GR cells, but the ALDH3B2 expression was decreased., Conclusions: ALDH might be used as a molecular biomarker to test the gefitinib-resistant to lung adenocarcinoma cancer cells, and the ALDH1A1 may play a role in gefitinib resistance in lung cancer.

Objective: To prepare human cystatin C( CysC) recombinant protein and produce monoclonal antibodies with high affinity and specificity. Develop a competitive ELISA detection system to detect of CysC in human serum., Methods: The CysC gene sequence was found on NCBI. The optimized gene fragments were synthesized and the recombinant CysC protein was expressed in Escherichia coli then used to immunize Balb/c mice. The positive hybridoma cell lines were obtained by hybridoma cell fusion techniques and ascites monoclonal antibody was prepared and purified. Affinity of the antibody was measured by indirect ELISA. Then competitive ELISA detection system was established, and 52 cases of human serum samples were detected by the detection system., Results: Four stable cell lines secreting CysC monoclonal antibodies were obtained. Antibody Ab3 was used as a detection antibody and HRP labeling was performed. Its affinity constant was 4. 26 × 10~6L/mol. The linear range of detection was 0. 011-1. 924 μg/mL. The detection limit was 4. 598 ng/mL and IC&#95;(50) was 0. 145 μg/mL. The established competitiveELISA serum detection system could accurately detect those 52 serum samples., Conclusion: The monoclonal antibody against CysC with high affinity and specificity has been successfully obtained. A reliable competitive ELISA serum detection system is established. The method provides a basis for the development of CysC rapid immunoassay kit.

Acute kidney injury (AKI) is a serious clinical problem with high morbidity and mortality. Renal replacement therapy (RRT) is an important tool for treating patients with AKI. The 2011 Kidney Disease: Improving Global Outcomes (KDIGO) Clinical Practice Guideline for AKI points out that RRT should be discontinued when renal function has recovered enough to meet the body needs or when RRT is no longer consistent with treatment goals. However, the specific reference index of weaning RRT is unclear. The guiding roles of traditional indicators such as urine output (> 400 mL/24 h), serum creatinine (SCr, decreasing trend), creatinine clearance (CCr, > 20 mL/min), and novel biomarkers such as neutrophil gelatinase-associated lipocalin (NGAL), hepatocyte growth factor (HGF), interleukins (IL-6, IL-10), kidney injury molecule-1 (KIM-1), kynurenic acid, etc. for discontinuation of RRT in AKI patients were reviewed. Particularly, the importance of biomarkers for this purpose was highlighted.