1. [Synthesis, characterization and evaluation of octadecyl ester-bonded monolithic column].
- Author
-
Wang L, Yang G, Li X, Li X, Yin J, Li Z, Gao W, Yang J, and Chen Y
- Subjects
- Anthracenes analysis, Benzene analysis, Biphenyl Compounds analysis, Chromatography, High Pressure Liquid methods, Methanol chemistry, Molecular Structure, Spectroscopy, Fourier Transform Infrared, Water chemistry, Chromatography, High Pressure Liquid instrumentation, Esters chemistry, Silanes chemistry, Stearic Acids chemistry
- Abstract
An ester-bonded monolithic silica column for reversed-phase high performance liquid chromatography was synthesized by reacting stearic acid with gamma-glycidoxypropyltrimethoxysilane and coupling the intermediate product onto monolithic silica column. Characterization of the prepared column was carried out with Fourier transformation infrared spectrometry and elemental analysis. The chromatographic properties of the packing were evaluated by using benzene, biphenyl, and anthracene as samples and methanol-water as binary mobile phase. The column back-pressure and separation effect were also evaluated for fast separation. The results show that the stationary phase has satisfactory chromatographic properties and resists hydrolysis between pH = 2 - 8, and it can be used for the fast-separation of solutes efficiently.
- Published
- 2005