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This paper summarized the nursing interventions for an elderly patients with severe malnutrition. Nursing interventions mainly included nutrition support, multidisciplinary team care, safety protection, etc. Comprehensive and targeted nursing interventions are required to ensure the safety during hospitalization, maintain patient's body function and improve the rehabilitation of the patient. (本文总结1例重度营养不良患者的护理经验, 包括补充营养、多学科团队照护、安全防范护理等内容。护理人员通过实施全面、针对性的优质护理服务, 确保患者住院期间安全, 维护患者机体功能, 促进患者早日康复。)

Objective: To discover the relationship of transcriptional levels and promoter methylation status of CHFR gene in human nasopharyngeal carcinoma,to discuss the significance and epigenetic mechanism of CHFR inactivation in NPC, and to evaluate the feasibility of detecting methylated CHFR in nasopharyngeal swab as a means for diagnosis of NPC., Method: Transcriptional levels of CHFR was evaluated by RT-PCR. Methylation specific PCR was used to detect the methylation status of CHFR in NPC cells, normal nasopharyngeal epithelia, primary tumors and their paired nasopharyngeal swabs. Detailed methylation status was confirmed by bisulfite sequencing. NPC cells were treated by the methyltransferase inhibitor 5-aza-dC and the reactivation of CHFR was evaluated by RT-PCR., Result: CHFR transcription was inactivated in NPC. The methylation frequency in NPC primary tumors and their paired swabs were 65.5% and 63.8%, respectively, with a 86.2% concordance. Bisulfite sequencing revealed a dense methylation in NPC cells and primary tumors, but all the normal nasopharyngeal epithelia were unmethylated. CHFR expression were restored after 5-aza-dC treatment., Conclusion: CHFR is epigenetically inactivated by promoter methylation in NPC. Detecting methylated CHFR can be served as a useful non-invasive means for diagnosis of NPC.