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Background and objective Bone is a common site for metastasis in lung adenocarcinoma, but the mechanism behind lung adenocarcinoma bone metastasis is still unclear. And currently, there is a lack of easily traceable and stable lung adenocarcinoma bone metastasis cell models, which limits the research on the mechanism of lung adenocarcinoma bone metastasis. The establishment of human lung adenocarcinoma cell line that are highly metastatic to bone, labeled with green fluorescent proteins (GFP) and fireflies luciferase (LUC), along with transcriptomic characterization, would be beneficial for research on lung adenocarcinoma bone metastasis and provide new experimental methods. Methods The human lung adenocarcinoma cell line A549-GFP-LUC was injected into nude mice via the left ventricle to construct a bone metastasis model, and was domesticated in vivo for three consecutive times to obtain the human high bone metastasis lung adenocarcinoma cell line A549-GFP-LUC-BM3; cell counting kit-8 (CCK-8), colony formation assay, scratch wound assays, Transwell assay and Western blot were used to compare the proliferation and invasion abilities of A549-GFP-LUC-BM3 with the parental cells. A549-GFP-LUC-BM3 cells and parental cells were further analyzed by transcriptomic sequencing. Results Human high-bone metastatic lung adenocarcinoma cells A549-GFP-LUC-BM3 was successfully established. Compared to parental cells, this cells exhibited a significantly higher incidence of bone metastasis and enhanced in vitro proliferation, migration, and invasion abilities. Transcriptomic sequencing results revealed that the A549-GFP-LUC-BM3 cell line had 2954 differentially expressed genes compared to the parental cells, with 1021 genes up-regulated and 1933 genes down-regulated. Gene Ontology (GO) functional enrichment analysis indicated that the differentially expressed genes were primarily localized in cellular components such as the cell periphery. The molecular functions identified as significantly enriched included signaling receptor activity, calcium ion binding, and extracellular matrix structural constituent. Additionally, the biological processes found to be enriched were cell adhesion and biological adhesion. The enrichment analysis conducted using the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that the differentially expressed genes were primarily involved in the metabolism of xenobiotics by cytochrome P450, retinol metabolism, drug metabolism-cytochrome P450, cell adhesion molecules, steroid hormone biosynthesis, and the nuclear factor kappa B (NF-κB) signaling pathway. Conclusion The highly bone-metastatic human lung adenocarcinoma cell line with GFP and luciferase double labeling was successfully established. The biological behavior and transcriptome sequencing of the cell line suggest that it has a high bone-metastatic potential.

Background and purpose: The plasma used for routine coagulation test (CCT) can only reflect a single component at a certain coagulation time point/segment, while thromboelastography (TEG) can depict the overall dynamic process curve of coagulation and fibrinolysis, which can more independently and completely reflect the true state of the blood and can serve as a supplement to coagulation function testing. This study aimed to evaluate the application value of combined coagulation function indexes in monitoring the hypercoagulable state of patients with colorectal cancer after chemotherapy, and to explore the risk factors of thrombosis in patients with colorectal cancer after chemotherapy, so as to provide reference for clinical monitoring of hypercoagulable state. Methods: A total of 160 patients with colorectal cancer from Fudan University Shanghai Cancer Center from June 2021 to June 2023 were selected as the experimental group, and 80 healthy subjects were selected as the control group. Then the experimental group was divided into a group without thrombosis (82 cases) and a group with thrombosis (78 cases) according to whether they had thrombosis or not. The determinations of thromboelastography (TEG) [coagulation reaction time (R), coagulation formation time (K), blood clot formation rate (α-Angle), maximum amplitude (MA) and coagulation index (CI)], conventional coagulation tests (CCT) [activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT), fibrinogen (Fib), D-dimer (DD), fibrinogen degradation products (FDP)] and platelet count (PLT) were studied among three groups. With or without thrombosis as the criterion of hypercoagulable state, statistically significant indicators were selected to be included in the binary logistic regression analysis, and the receiver operating characteristic (ROC) curve was drawn to analyze the diagnostic efficacy of single and combined detection of the coagulation function indicators for hypercoagulable state in patients with colorectal cancer after chemotherapy. Basic information, tumor stage and Autar score of deep vein thrombosis were collected in 160 patients with colorectal cancer. Logistic regression analysis was performed to explore the risk factors of thrombosis. This study was approved by the Ethics Committee of Fudan University Shanghai Cancer Center (number: 050432-4-2108*). Results: Compared with the control group, the R, TT and PLT of the group with thrombosis were decreased (P

【Objective】The bHLH transcription factor family is an important family of transcription factors in eukaryotes, which plays an important role in plant growth and development, secondary metabolism and stress response. Analysis of the response pattern of bHLH transcription factor gene OsbHLH109 (LOC_Os01g67480) to stress and hormones in rice (Oryza sativa L.) could provide a theoretical basis for further researches on the functions of OsbHLH109 in stress and hormone responses.【Method】The bioinformatics of OsbHLH109 was analyzed, and the expression patterns of OsbHLH109 in different tissues of Rice Zhonghua 11 under different hormone and abiotic stresses were analyzed by quantitative PCR (qRT-PCR).【Result】The encoding region of OsbHLH109 was 1 164 bp in length, containing 6 exons and encoding 388 amino acids, which was a bHLH transcription factor containing a conserved domain of HLH. Phylogenetic analysis showed that OsbHLH109 was closely related to the homologous proteins in monocots and Dicotyledones. The analysis of cis-acting elements showed that the promoter region of OsbHLH109 contained 24 plant hormone response cis-elements and 25 environmental stress response cis-elements. The qRT-PCR analysis showed that OsbHLH109 was expressed in roots, stems, leaves and young panicles of rice, but the expression level was the highest in leaves. The results of hormone treatment response expression profile analysis showed that the response expression differences of OsbHLH109 in leaves to 6-Benzylaminopurine (6-BA), indole acetic acid (IAA), gibberellic acid (GA3) and abscisic acid (ABA) treatments all reached significant levels. The results of stress treatment response expression profile analysis showed that the response of OsbHLH109 to low temperature stress, drought stress, high temperature stress and salt stress were significantly different in leaves, indicating that the expression of OsbHLH109 was induced by 6-BA, GA3, IAA, ABA, high temperature, low temperature, PEG6000 and NaCl, which suggested that OsbHLH109 was essential for the abiotic stress responses in rice.【Conclusion】Rice OsbHLH109 is a member of the bHLH transcription factor family and is highly expressed in leaves. The expression of OsbHLH109 gene is regulated by high temperature, low temperature and salt stress, and responds to hormone signals such as 6-BA, IAA, GA3 and ABA. It is predicted that it may be involved in abiotic stress response of rice through ABA and other hormone signal transduction pathways.

In this paper, the theoretical innovation is made on the traditional fire distribution problem. Considering the actual situation of the ship’s fault, based on the principle of multi-objective optimization, the improved distributed genetic algorithm is used for preliminary solution. And the corresponding fault-tolerant control law is formulated to correct the fault-tolerant strategy, so as to ensure the better fire strike effect and maintain a high fault tolerance. Finally, through simulation experiments, it is verified that this method can converge to the optimal solution quickly, and can reduce the loss to the maximum extent when compensating. It has certain research value for combat.

The objective of this study was to optimize the extraction process of total flavonoids from Haloragis micrantha (Thunb.) R. Brown. and explore its antioxidant activity. With the yield of total flavonoids as an evaluation index, single factor tests were used to investigate the effects of ethanol volume fraction, solid-liquid ratio, ultrasonic temperature and extraction time on the total flavonoids content of Haloragis micrantha (Thunb.) R. Brown., and the Box-Behnken method was used to optimize the extraction process. The scavenging ability and total reducing power of total flavonoids of Haloragis micrantha (Thunb.) R. Brown. to DPPH and ABTS+ free radicals were detected. The results showed that optimum extraction process was ethanol volume fraction of 76.15%, liquid to material ratio of 1:39.66 g/mL, ultrasonic time of 41.14 min. The yield of total flavonoids from Haloragis micrantha (Thunb.) R. Brown. was 28.61±0.05 mg/g. The total flavonoids of Haloragis micrantha (Thunb.) R. Brown. could effectively scavenge DPPH and ABTS+ free radicals. Within a certain range, the scavenging ability increased with the increasing of mass concentration, and the total flavonoids of Haloragis micrantha (Thunb.) R. Brown. had high reducing power and better antioxidant activity. The total flavonoids of Haloragis micrantha (Thunb.) R. Brown. had the strongest ability to scavenge ABTS+ free radicals. When the concentration of total flavonoids was 3 mg/mL, the clearance rate of ABTS+ were 98.28%, which was equivalent to 3 mg/mL vitamin C (clearance rate was 99.81%). This process would be simple and feasible, and the extracted total flavonoids have good antioxidant activity.

To study the effect of Annulohypoxylon stygium fermented tea aqueous extracts (ASTAE) on blood lipids and liver protection in mice, 80 male KM mice were established hyperlipidemic model by high-fat diet induction, and then randomly divided into eight groups: blank control group (CK), model group (HF), positive control group (UA, 200 mg/kg), green tea group (GT, 400 mg/kg), Annulohypoxylon stygium group (AS, 400 mg/kg), and high (HA, 400 mg/kg), medium (MA, 200 mg/kg) and low (LA, 100 mg/kg) dose Annulohypoxylon stygium fermented tea aqueous extracts groups. After 5 weeks, serum total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum creatinine (Scr), blood urea nitrogen (BUN) and adiponectin (ADPN) levels were detected. Detect the organ index, and liver fat lesions and distribution were observed by HE and oil red O staining. The results showed that compared with the HF group, the HA group could extremely significantly reduce the levels of TC, TG, LDL-C, AST, ALT, BUN and Scr in hyperlipidemia mice (P

Protein is an important nutrient required by the human body. The change of protein structure during processing will lead to changes in its functional properties, in turn affecting the quality of foods. There are many physical methods available to alter the structure of proteins to expand their application in the food industry. The new electrophysical processing technology has become a hot spot in the field of food processing due to its advantages of high efficiency, low energy consumption, and slight loss of nutrients. Therefore, this paper reviews the effects of electric field technology (ohmic heating and electrostatic field) and electromagnetic field technology (microwave, radio frequency and magnetic field) on the change of protein structure at multiscales (macroscopic, molecular and microscopic levels), in order provide a theoretical basis for the development and utilization of electromagnetic field processed protein products.

This study aimed to investigate the impacts of vegetables with different textures on food oral processing behavior, food intake and satiety. Broccoli cooked by microwave for different periods of time were tested in this study. The oral processing behavior of simultaneous and sequential consumption of cooked broccoli and rice, ad libitum food intake and postprandial satiety were measured in healthy subjects. Results showed that compared with co-ingestion of soft broccoli and seasoned rice (SSR), sequential ingestion of hard broccoli followed by seasoned rice (H + SR) increased the number of chews, the oral sensory exposure time and the number of chews per gram by 44%, 34% and 57%, respectively, and reduced the eating rate by 25% and the rice intake by 20%. Moreover, compared with SSR, H + SR effectively suppressed the perception of postprandial hunger. In conclusion, hard-cooked broccoli helped to reduce food intake and inhibit subjective hunger by enhancing oral processing, and this effect was more significant when the vegetable was first consumed cooked by cooked.

ObjectiveTo investigate the willingness to pay (WTP) for human papillomavirus (HPV) vaccine and its influencing factors among Chinese female health care workers for improving HPV vaccination strategies in China. MethodsAn online survey was conducted among 18 – 45 years old female health care workers from 310 hospitals in 31 provincial level administrative divisions across China during November – December 2021. A self-designed questionnaire was used to collect information on demographics, HPV vaccination status, HPV-related knowledge, protective motivation theory (PMT)-based HPV vaccination-related cognition and attitude, and the amount of WTP for HPV vaccination. Structural equation model was adopted to analyze influencing factors of WTP. ResultsOf the 15 969 valid respondents aged 30.6 ± 6.2 years, 43.4%, 33.2%, and 23.4% reported the highest amount (CNY) of WTP for HPV vaccine ranging 0 – 1 600, 1 601 – 3 200, and 3 201 – 5 000, respectively; higher amount of WTP was reported by the respondents aged 18 – 26 years, being Han ethnic, unmarried/divorced/widowed, having the education of master degree or above, without professional title, with a monthly income more than 8 000 CNY, working in a tertiary hospital, and already receiving HPV vaccination or having an appointment for the vaccination. The scores for multidimensional PMT-based cognition and attitude about HPV vaccination were significantly correlated with HPV vaccine acceptance and the WTP for HPV vaccine (P < 0.001 for all). The structural equation model analysis showed that perceived susceptibility (β = 0.046), perceived severity (β = 0.012), response efficacy (β = 0.027), self-efficacy (β = 0.155), HPV-related knowledge (β = 0.151) and vaccination behavior (β = 0.154) were positively associated with the WTP for HPV vaccination; whereas response cost was negatively associated with the WTP for HPV vaccination (β = – 0.183). ConclusionChinese female health care workers have a relatively higher WTP for HPV vaccination. Price of HPV vaccine, convenience of vaccination/ appointment services, and safety of vaccines remain the main influencing factors of the WTP.

With the expansion of the fields covered by AI, artistic creation will become the next hot spot for AI research and applications.Building a metaverse with diverse styles, realistic contents, flexible strokes and accurate descriptions based on parallel system theory and ACP approach will provide a feasible way to improve AI creation capability.The intelligence of human, AI, and robots were fused to develop a parallel creation architecture through the creation by AI, the evaluation by humans, and the execution by robots.The parallel creation with the key methods of style transfer, content combination, stroke generation and image captioning in computational experiments were explained.The parallel creation system was validated through the painting experiments.The parallel creation system will improve the creation capability of artificial intelligence in cyberspace and physical space, and promote the human-cyber-physical collaborative creation through the fusion of them.

ObjectiveIn order to improve the charging efficiency, safety and speed of mobile online photovoltaic (PV) charging devices, a type of mobile online PV charging maximum power point tracking (MPPT) technology based on the variable step size disturbance approximation method is proposed. MethodsBy combining a high-frequency inverter and isolation transformer as the MPPT carrier, the former DC/DC structure of traditional PV charging topology devices is removed, charging control is combined with the pulse modulation of the high-frequency inverter, and the three functions of high-frequency isolation, MPPT tracking and charging control are finally integrated into one. At the same time, according to the different states of the remaining power battery, a three-stage charging control strategy is proposed.ResultsSimulation analysis and field test results show that this device can track the maximum power points of PV panels stably when their illumination intensity changes, and the battery can be charged efficiently in the three stages according to the set charging scheme. The measured overall charging efficiency can be maintained above 92%.ConclusionThis device and control strategy can improve PV panel-to-battery charging efficiency and meet the requirements of fast and safe charging.

ObjectivesIn order to realize the rapid data exchange of the monitoring module in a grid-connected control system, the fault tolerance rate of communication data is improved and the extra CPU occupancy is saved by using Direct Memory Access (DMA) and interrupt communication technology to solve the problem of variable length transmission.MethodsThis paper is based on the communication protocol between the configuration software of the monitoring module and the microcontroller in a grid-connected control system. It designs a communication handshake flow between configuration and main controller, taking STM32F417 as the main controller, with the help of a serial interface hardware circuit. The use of DMA and interrupt communication technology is proposed to overcome the shortcomings of the traditional serial communication data fixed-length transceiver.ResultsOn the grid-connected control device of a ship's integrated power system, DMA and interrupt communication technology is adopted to realize the variable-length transmission function of the monitoring data, thereby improving the serial communication data transmission capacity and data tolerance.ConclusionsThe results of this study have certain practical guiding significance and reference value for the development of monitoring modules for ship grid control systems.

The development of society and the growth of public cultural needs bring new challenges to the operations and management of museums in the new era.Considering the current state of the museum management and its challenges in exhibition, education, and safety, the parallel museums framework as a new solution to the museum management was proposed, and technical reference for constructing intelligent museum was proposed.The parallel museums framework was the application of ACP theory in museum operations and management, descriptive intelligence was used to construct a virtual museum, predictive intelligence was used to do large-scale computational experiments in the virtual museum, and prescriptive intelligence and parallel execution were used to control the real museum.

ObjectivesIn order to improve the reliability of shipborne electrical plant control systems and solve the key problem of fault detection in dual CPU hot backup redundant communication, a comprehensive set of fault detection mechanisms is proposed.MethodsAfter analyzing traditional dual CPU fault detection, this study proposes a hybrid fault detection mechanism combining the network fault detection method with the third-party monitoring heartbeat method. Based on the designed dual CPU redundant system architecture, the principles and software implementation of the two proposed fault detection methods are analyzed, and the proposed fault detection method is verified on an actual device.ResultsAfter actual device inspection, the results show that the proposed dual fault detection technology overcomes the shortcomings of traditional fault detection methods such as focusing on CPU faults but ignoring CPU peripheral communication faults, and susceptibility to the "dual host phenomenon". It also improves the speed and accuracy of fault detection to ensure the stable and reliable operation of the dual CPU redundant system.ConclusionsThe results of this study can provide references for the dual CPU redundant communication fault detection methods of shipborne electrical plant control systems.

Objectives To address the unstable power output of photovoltaic systems on the mobile platforms of ships and power fluctuations caused by surge loading/unloading, an energy management strategy involving a photovoltaic hybrid energy storage system based on optimal power distribution is proposed. Methods In view of the shortcomings of the traditional limit management method, the filter time constant is adaptively adjusted according to the different zones of the charged state of the supercapacitor, thereby realizing the optimal power distribution of the hybrid energy storage system. Meanwhile, the control strategy and working mode of each converter are analyzed. Results The Matlab simulation results and engineering test results show that the energy management scheme and converter control strategy can effectively improve the overcharge or over-discharge problem of hybrid energy storage systems, not only ensuring the stability of the DC bus voltage, but also improving the dynamic response and coordination of the system. Conclusions The results of this study can provide references for the energy management of photovoltaic systems on mobile platforms.

The artistic creation of machine has drawn considerable attention and achieved significant development in recent years.There are more and more artworks processed partially by specific algorithms,or even created entirely by machines.Despite their popularity,it is hard for these artworks to be accepted by humans because of their underwhelming sensory impacts and lack of empathy.However,their impact is highly concerned by the art community.A theoretical framework called the parallel art system was proposed to solve the technical and human-machine-relationship challenges in artistic creation.The system aims to build a partnership between humans and machines,enabling them to collaborate in a parallel manner,and even provide a new way to integrate human emotion and machine logic.

A novel maximum-minimum eigenvalue (NMME) cooperative spectrum sensing algorithm and threshold decision rule are proposed via analyzing minimum eigenvalue limiting distribution of the covariance matrix of the received signals from multiple cognitive users (CU) by means of latest random matrix theory (RMT).The proposed scheme could not need the prior knowledge of the signal transmitted from primary user (PU) and could effectively overcome the noise uncertainty.At a given probability of false alarm (Pfa),simulation results show that the proposed scheme can get lower decision threshold and higher probability of detection (Pd) compared with the original algorithm,and it can also get better detection performance with fewer CU and smaller sample numbers.

A novel GLSFBC (group layered SFBC)-CDMA-OFDMA multiple antenna transmit scheme was proposed over frequency-selective fading channels.The proposed scheme is based on a three-level design of cascade codes:the first level(based on OFDMA)deals with multi-user interference and frequency-selective fading,the second level(based on CDMA) handles group interference and obtains frequency diversity gain,and the third level (based on GLSFBC) results in space diversity and multiplexing gain.At the receiver,the proposed approach only needs one antenna to suppress both multi-user interference and SFBC group interference,so it makes the receiver construction simple.Both theory analysis and computer simulation prove the validity of the novel technique.

The activated municipal solid waste incineration bottom ash was prepared and taken as raw material, supplemented with pretreated bottom ash, cement, sand and additive to pressurize unburned bricks. Under the certain fineness of the activated bottom ash, the influence of the raw material ratio, water-binder ratio and curing conditions on the mechanical properties of the bricks were analyzed. Moreover, the reaction mechanism of unburned bricks were further discussed. The results showed that: feldspar are reduced, and organic carbon are decomposed, forming a higher internal amorphous structure. C-S-H gel of unburned bricks and hydration products were mixed and polymerized, forming compact structure with high mechanical properties. Under the formula of activated bottom ash 45%, pretreated bottom ash 25%, cement 11%, admixture 2%, sand 17%, the optimization process conditions of unburned bricks were 14 h curing time at 105²C and m of activated bottom ash as well as 0.2 water-to-binder ratio. The performance standards were in accordance with the requirements of JC/T 422-2007"non-sintered waste tailing brick"MU15. [ABSTRACT FROM AUTHOR]

The solidification of waste incineration bottom ash and inorganic cement is a solid waste conversion treatment. Cement, lime, gypsum with different dosages were used as curing agents to change the amount of waste incineration bottom ash, and the bottom ash solidification test was carried out. The unconfined compressive strength and heavy metal leaching amount of the solidified block were tested, XRD and SEM analysis were carried out. The test results show that when the solidified material of the waste ash is 100% waste incineration bottom ash,25% cement and 25% gypsum,the maximum unconfined compressive strength is 18.5 MPa, and the strength of the solidified block increases with the curing time. The solidified block meets the quality requirements of category IE soil environment,and the leaching toxicity is lower than the environmental quality requirement of category V standard surface water, and can be used as masonry material. [ABSTRACT FROM AUTHOR]

The manganese (Mn 4 ) cluster, as a part of the oxygen-evolving complex (OEC) in the photosystemⅡ (PS Ⅱ) of microalgae and plants, assists in the electrolysis of water to oxygen, protons, and electrons. To examine the relationships among manganese (Mn) concentrations in the culture medium, algae growth, and chlorophyll fluorescence characteristics, we exposed the diatom Conticribra weissflogii to a broad range of Mn concentrations from 0 to 9000 nmol·L -1 . Chlorophyll fluorescence induction dynamics analysis, an effective way to investigate photosynthetic characteristics, can be used as indicator of the photosynthetic apparatus of photosynthesizers under different stressors. Here, we studied the effects of Mn exposure on C. weissflogii using this fluorescence analysis method. The results show that the growth of C. weissflogii is independent of the Mn exposure at concentrations below 9000 nmol·L -1 . Moreover, chlorophyll fluorescence parameters of C. weissflogii respond to exposed Mn concentrations, whereby the strongest induction occurred at 90 nmol·L -1 Mn and the responses are enhanced over time. Although Mn in culture media may not be a major limiting factor of the growth of C. weissflogii , it significantly enhances the photosynthesis of C. weissflogii via two ways. First, Mn improves the integrity of the OEC structure and electron transfer from OEC to Tyr on the donor side of PS Ⅱ; second, Mn also enhances the energy transfer and electron transport after reaching the primary quinone acceptor ( Q A ) on the acceptor side of PS Ⅱ. Energy transfer-related fluorescence parameters are positively correlated with the level of ROS in C. weissflogii , indicating that Mn plays an important role in both photosynthesis processes and reactive oxygen species(ROS)production.

This article reports one hepatic echinococcosis patient complicated with systemic sclerosis. His clinical manifestations were the progressive fibrosis of the skin, sour regurgitation, and belching. The blood examination showed that eosinophils was reduced, and antinuclear antibody (ANA) was positive at 1:100 in cytoplasm particle type. He was given prednisone acetate 25 mg, q. d., aspirin 100 mg, q. d., centella triterpenes cream 12 mg t. i. d., esomeprazole 40 mg q. d., and domperidone 10 mg t. i. d. After one week, the Rodnan skin score reduced from 27 to 17. The liver hydatid cyst resection was performed, and the follow-up showed that his clinical manifestations improved and the Rodnan skin score reduced further.

Objective: To investigate the inductive therapeutic effects of imatinib combined with VP low dose regiment on adult patients with Ph-positive acute lymphoblastic leukemia (Ph(+) ALL)., Methods: Fourteen newly diagnosed adult patients with Ph(+) ALL were treated with VP regimen, and imatinib (400 mg/d) was added at the 8(th) day. VP regimen would be stopped when neutropenia lasted for 1 week or complicated with infection, fever, etc. Therapeutic effects were assessed by bone marrow morphology and quantitative analysis of BCR/ABL:ABL at the 28(th) - 33(rd) day. Patients could be treated with imatinib combined with chemotherapy for consolidation and maintenance therapy or were treated with allogeneic hematopoietic stem cell transplantation after complete remission., Results: Fourteen cases obtained CR1 after first course of treatment, the median decline of BCR/ABA:ABL was 55.89 (10.25 -180.97) %; during the induction chemotherapy, pulmonary infection occurred in 3 patients, diarrhea in 1 patients, facial edema in 3 patients, however, all these patients were cured after symptomatic treatment, only 1 patient died of relapse after transplantation., Conclusion: In the period of tyrosine kinase inhibitor (TKI), inductive chemotherapy combined with imatinib and low dose VP can obtaine satisfactory CR rate and decrease the toxicity of the traditional drugs. It is suggested that TKI combined with VP regimen chemotherapy can achieve CR1 and make possible for allo-HSCT, from which patients can achieve the long-term survival.

Engineered nanoparticles (ENPs) have shown invaluable societal benefits and applications in drug targeting, biological imaging and industrial products. ENPs enter the water body through various paths during the processes of production, usage and emission, therefore the behavior and the biosafety of ENPs in water bodies have attracted increasing attention. As the primary producer of ecosystems, phytoplankton provide nutrients, energy and oxygen for both themselves and organisms at higher trophic levels in the aquatic ecosystems. These primary producers may be exposed to the biological and unpredictable effects of this emergent pollutant to the aquatic ecosystems. Numerous studies have proved the toxic effects of ENPs on phytoplankton, but the mechanisms of entry into the aquatic organisms as well as the stability, fate and biotransformation in phytoplankton still remain unclear. Here, we present a review of the pathways of ENPs entering the water, the subsequent behavior and biological effects of ENPs on phytoplankton with an emphasis on latest findings and current knowledge. Future research and endeavors shall focus further on the understanding of mechanisms, fate and transport of ENPs in the aquatic ecosystems.

The aim of this study was to investigate the clinical characteristics and prognosis of acute erythroleukemia (AEL, AML-M6). The clinical features and results of morphologic, immunophenotypic, cytogenetic and molecular biologic detections were retrospectively analyzed in 13 cases of AEL from 305 acute leukemia patients hospitalized between October 2007 and October 2012. The results showed that the expression of erythroid and non-erythroid cells increased at the same time. The myeloid antigens mainly expressed CD13/CD33/CD117/CD34, while the erythroid antigens expressed Gly and CD71. The karyotypic detection indicated that there were 1 case with normal karyotype, 3 cases with simple karyotypic abnormality and 2 cases with complex karyotypic abnormality, the other cases were not detected. The molecular biological detection found that the poor prognosis gene existed in 5 cases [38.5% (5/13)], including 3 cases with MLL-MLL fusion gene, 1 case with MLL mutation, and 1 cases with NRAS gene mutation, the abnormal genes were not detected in remainder 8 cases. After chemotherapy with decitabine, the complete remission (CR) rate achieved 53.5% (7/13), partial remission (DR) rate achieved 15.4% (2/13). Finally, 8 patients received allo-HSCT, the median overall survival (OS) was 20.7 months, 3 year survival rate was 79%, 3 year disease-free survival rate was 78%. It is concluded that the acute erythroleukemia is a rare subtype of AML, which is transformed from MDS and has harmful genes and poor prognosis. Allo-HSCT and treatment with decitabine may enhance the survival rate of AEL.

The purpose of this study was to investigate the efficacy of autologous hematopoietic stem cell transplantation (auto-HSCT) for patients with diffuse large B-cell lymphoma (DLBCL), and analyse the factors influencing prognosis. The clinical data of 67 patients with DLBCL received auto-HSCT from 1996 to 2011 and cumulative overall survival (OS), progression-free survival (PFS), transplant-related mortality (TRM), and relapse rate were retrospectively analyzed. The results showed that the median follow-up time was 40 months after transplantation. Three-year cumulative OS and PFS were 70.6% and 66.4% respectively, 5-year cumulative OS and PFS were 70.6% and 63.8% respectively, and TRM was 7.2%. One-year and three-year cumulative relapse rate were 16.5% and 23.7% respectively. Univariate analysis revealed that the age and pre-transplant disease status were significantly associated with poor prognosis (P < 0.05). It is concluded that auto-HSCT is a safe and effective therapeutic option for patients with DLBCL, especially for the young patients or patients with better remission.

Diffuse large B cell lymphoma (DLBCL) is the most common aggressive non-Hodgkin's lymphoma (NHL), characterized by great heterogeneity in clinical manifestations and molecular genetics. This study was aimed to explore the clinical significance of applying multiplex PCR to detect BCL2/IGH and BCL6/IGH fusion genes in DLBCL. Multiplex PCR was used to detect bone marrow samples from 80 cases of DLBCL. The results showed that 12 patients (15%) carried BCL2/IGH or BCL6/IGH fusion genes, BCL2/IGH was found in 6 patients (7.5%), and BCL6/IGH in another 6 patients (7.5%). The patients with different molecular markers displayed different clinical features and outcomes. It is concluded that multiple PCR is rapid and accurate method to identify gene abnormalities in DLBCL, but further studying a quantitative or semi-quantitative assay for the expression of fusion genes is needed.

This study was aimed to investigate the clinical value of multiplex nested reverse transcription PCR (RT-PCR) in detecting MLL-related fusion genes in myelodysplastic syndrome (MDS). Ten MLL-related genes (dupMLL, MLL-ELL, MLL-ENL, MLL-AF6, MLL-AF9, MLL-AF10, MLL-AF17, MLL-CBP, MLL-AF1P, MLL-AF1Q) in 221 MDS cases were detected by multiplex nested RT-PCR. The results indicated that 20 patients were detected with positive result among 221 patients and the positive rate was 9.05%. The number of the positive cases and positive rates of the above mentioned 10 fusion genes were in order: 7 (3.16%), 2 (0.9%), 1 (0.45%), 1 (0.45%), 2 (0.9%), 2 (0.9%), 1 (0.45%), 2 (0.9%), 1 (0.45%), 1 (10.45%). It is concluded that the multiplex nested RT-PCR has been confirmed to be able to detect 10 fusion genes in MDS patients, which can provide important evidences for assessing diagnosis and treatment, and give related necessary information about minimal residual disease and its prognosis.

By using pitfall trapping and ocular estimation, this paper studied the effects of Solenopsis invicta invasion on the diversity of spider communities in a corn field. A total of 16 spider species belonging to 8 families were recorded. In the plot S. invicta invaded, there were 10 spider species of 6 families, among which, 5 species of 4 families were in pesticide-treated plot, and 11 species of 8 families were in control plot. The spiders were dominated by wandering species, mainly belonging to the families Salticidae and Oxyopidae, and the dominant species were Oxyopex sertatus, Myrmarachne joblotii, and Myrmarachne sp. In the plot S. invicta invaded, the population quantity of S. invicta began to increase steadily from the late whorl stage of corn, with the peak (225 individuals) through the earring stage, but decreased steadily thereafter, with the decrement of the spiders of genus Oxyopex reached 79.2%, while the population quantity of the spiders of genus Myrmarachne had no significant change. With the growth of corn, the species number, diversity index, and evenness index of the spiders in S. invicta-invaded and pesticide-treated plot decreased and the predominant indices increased gradually, while the situation in control plot was in adverse. It was concluded that due to the S. invicta invasion in corn field, the community structure of spiders changed.

Objective: To study the function and prognostic value of the recently identified tumor suppressor gene LKB1 in human breast cancer., Methods: Estrogen receptor-positive human breast cancer cells of MCF-7 line and estrogen receptor-negative human breast cancer cells of the lines MDA-MB-435 and MDA-MB-231 were cultured and tested for the LKB1 expression. Plasmid pcDNA4 TOPO HisMAX/LKB1 expressing LKB1 was constructed and transfected into the MDA-MB-435 cells. PCR and Western blotting were used to detect the expressions of LKB1 mRNA and protein. Flow cytometry was used to determine the cell cycle. PCR and Western blotting were used to detect the expressions of LKB1 mRNA and protein in 121 human breast cancer samples. The association with relapse free survival (RFS) and overall survival (OS) was assessed by univariate analysis (log-rank test and Kaplan-Meier method)., Results: Human breast cells of MCF-7 line expressed LKB1 and the cells of MDA-MB-435 and MDA-MB-231 lines did not. Flow cytometry showed that 40.9% of the MDA-MB-435 transfected with pcDNA4 TOPO HisMAX/LKB1 was at the G(1) stage, a proportion significantly higher than that of the control (18.3%). The expressions of cyclin D1 and cyclin E were significantly down-regulated and the expression of p21(WAF1/CIP1) was significantly up-regualed. LKB1 was lowly expressed in 43 out of the 121 cases of breast cancer (35.4%) and highly expressed in 78 cases (64.6%). Low expression of LKB1 was correlated with pathological grade (P = 0.013), cancer size (P = 0.001), status of estrogen receptor (P = 0.048), and status of lymph nodes metastasis (P = 0.003). Univariate analysis showed that low expression of LKB1 was significantly correlated with shorter RFS (P = 0.048) and low OS (P = 0.003)., Conclusion: Reintroducing LKB1 into breast cell of the lines lacking LKB1 expression restores LKB1 activity and induces growth suppression by G(1) cell cycle block. The LKB1 mediated G(1) cell cycle arrest is caused by up-regulation of the expression of p21(WAF1/CIP1) in MDA-MB-435 breast cancer cells.