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Prior to clinical application, reliability of percutaneous ventricular assist devices(pVAD) requires to be tested systematically. Currently, there's a lack of dedicated reliability testing equipment and methodologies for pVAD. Considering the structural and functional aspects of percutaneous ventricular assist devices, this study conducts research on pVAD reliability test engineering. Test setups, clinical conditions, failure modes, effects analysis, and evaluation models have been investigated. A highly feasible methodological approach for percutaneous ventricular assist device reliability assessment has been formed. This study offers valuable insights into standardizing their reliability evaluation in clinical settings.

The precipitation Extreme Forecast Index (EFI) derived from ECMWF global ensemble prediction system (EPS) from June 2016 to May 2017 is applied to evaluate the statistical relationship between the EFI and the severe precipitation as well as the climatic precipitation percentile in Zhejiang. Results show that there is an obvious positive correlation between the EFI and the observed precipitation in Zhejiang. With the increase of the EFI threshold, the frequency of rainstorm increases at first and then decreases, and the probability of rainstorm increases with the increase of EFI threshold. With the increase of the EFI, the frequency of rainstorm increases at first and then decreases, while the probability of rainstorm increases with the increase of the EFI. Considering the TS and BS scores comprehensively, the threshold of the EFI decreases with the increase of forecast time length and increase with the increase of precipitation. There is a significant positive correlation between the EFI value and the climatic percentile of precipitation. The greater the EFI is, the higher the percentile is. Thus, the precipitation amount can be estimated according to the corresponding climatic percentile of the observed precipitation.

Background Among people with various mental illnesses, those with schizophrenia have the highest incidence of aggressive behavior, which not only disturbs the medical environment, but also poses a great threat to family stability and social harmony. Previous regression analysis studies have shown that childhood trauma, self-esteem and resilience are influencing factors of aggressive behavior in schizophrenia patients, but few studies have explored the internal relationship between these four variables. Objective To explore the relationship between childhood trauma, self-esteem, resilience and aggressive behavior in schizophrenic patients using a self-constructed chain mediation model, and to clarify its internal mechanism. Methods A convenience sample of stable schizophrenia inpatients were selected from Daqing Third Hospital and Chifeng Anding Hospital from March 2021 to July 2022. A survey was conducted with them using a questionnaire consisting of general information questionnaire, Positive and Negative Symptom Scale (PANSS) , Childhood Trauma Questionnaire-Short Form (CTQ-SF) , Connor-Davidson Resilience Scale (CD-RISC) , Self-esteem Scale (SES) , and Aggressive Questionnaire (AQ) . Pearson correlation analysis was used to explore the correlation of AQ score with CTQ-SF, CD-RISC and SES scores. Multiple linear regression analysis was used to explore the influencing factors of aggressive behavior. AMOS 24.0 was used to construct the chain mediation model. The mediation effect of the chain mediation model was tested using Bootstrap method. Results Three hundred and ten cases attended the survey, and 300 of them (96.8%) who responded effectively were finally included for analysis. The average scores of PANSS, CTQ-SF, SES, CD-RISC and AQ were (18.4±3.7) , (49.7±13.5) , (26.9±6.9) , (55.2±15.5) and (84.3±19.8) , respectively. Multiple linear regression analysis showed that childhood trauma〔B=0.551, 95%CI (0.421, 0.681) , P

Objective To establish a rapid screening method for in vitro site-directed mutants or CRISPR/Cas9-mediated knock-in mutants, and to improve mutant screening efficiency and reduce experimental costs. Methods Polymerase chain reactions (PCRs) were carried out with primers designed with the last one, two, and three nucleotides in the 3' end matching the mutated DNA sequences for the constructed in vitro site-directed mutants or CRISPR/Cas9-mediated knock-in mutants, and agarose gel electrophoresis was used to detect specific amplification bands. Results PCR using specific mutation screening primers effectively amplified DNA bands in correct mutants but failed in unsuccessfully mutated samples. The presence of DNA amplification bands revealed by agarose gel electrophoresis confirmed the successful screening for desired mutants. Conclusion The rapid screening method for mutants introduced in this study can easily and quickly identify correct mutants, which significantly simplifies the identification process of mutations, and thereby reduces experimental costs and improves work efficiency.