Search

Objective: A novel electrochemical sensor with high sensitivity was developed for the quantitative analysis of rutin in tartary buckwheat. Methods: A layer of titanium dioxide was loaded on the surface of ferric oxide nano materials (Fe3O4) to prepare core-shell Fe3O4@titanium dioxide (Fe3O4@TiO2) nano composites, and nano gold (Au) was loaded on its surface for preparing a new type of Fe3O4@TiO2@Au nanocomposites. Then an electrochemical sensor was constructed for quantitative analysis of rutin. The optimal working conditions of the sensor were determined by electrochemical characterizing of nano materials, rutin and optimizing, buffer pH, enrichment time, enrichment potential and other conditions. Results: Fe3O4@TiO2@Au had good adsorption and conductivity, which could effectively improve the sensitivity of the sensor. Rutin concentration and its corresponding oxidation peak current show linear relationship in the concentration range of 0.02~20 μmol/L and 20~200 μmol/L, respectively. The detection limit (S/N=3) was 0.006 4 μmol/L, and the linear range was wide and detection limit was low. It is obviously better than other electrochemical sensors, and the sensor prepared had good repeatability, stability and good anti-interference ability to common co-existing objects. Conclusion: The prepared sensor had the advantages of simple operation, low cost and the potential of recycling of nanocomposites, which provides a more effective method for rapid quantitative analysis of rutin.

Objective To identify the causative agent of an acute diarrhea outbreak in a school in Taian, Shandong, and to analyze the genetic characteristics of the pathogenic virus. Methods A total of nineteen samples including anal swabs from the diarrheal patients and related staff in the school canteen and environmental samples were collected. These samples were tested for common pathogens causing viral diarrhea by quantitative real-time PCR (qPCR), followed by high-throughput sequencing to obtain the viral genome sequences of the qPCR positive samples. Genetic characterizations of the causative agent were further studied including nucleotide and amino acid sequence identity and phylogenetic analysis. Results Seven of the 19 samples tested positive for adenovirus by qPCR, with the positive rate of 36.8%. Whole-genome sequences of human adenovirus serotype 41 (HAdV-F41) were obtained from the anal swab of Patient 10 and the tableware of Patient 4, with the nucleotide and amino acid identity of 100% between them. Phylogenetic analyses of the full-length genome, hexon gene, and fiber gene sequences showed that the HAdV-F41 strain discovered in this study had high homology with strains previously described from China, the United States, Japan, Iraq, and other countries. Conclusion Our findings indicated that HAdV-F41 was the causative agent responsible for the acute diarrhea outbreak in Taian, which shared high homology with previously reported HAdV-F41 strains worldwide. These results enriched the genetic resource of HAdV-F41 in Shandong and provided a theoretical basis for the prevention and control of acute diarrhea in children caused by adenoviruses.

Objective To summarize the clinical, imaging and genetic characteristics of spinocerebellar ataxia 2 (SCA2) families presenting as Parkinsonism. Methods and Results Two families with autosomal dominant SCA2 with Parkinson's syndrome diagnosed by genetic test in Peking University First Hospital from May 2019 to December 2020 were included. The probands in 2 families manifested as lead⁃pipe like rigidity and cerebellum ataxia. Electronystagmography (ENG) in proband of family 1 showed gaze nystagmus, understable saccade, decreased smooth tracking ability and disappeared optokinetic nystagmus, proband of family 2 showed slow saccade, decreased smooth tracking ability and decreased optokinetic nystagmus. Head MRI in both probands of 2 families showed slightly enlarged third ventricle, while the proband of family 2 showed atrophy in brain stem and cerebellar. Whole exome sequencing (WES) in 2 probands showed no gene mutations, Sanger sequencing showed dynamic mutation of ATXN2 gene and the copy number of cytosine⁃adenine⁃guanine (CAG) with 37 repeats. Two probands were finally diagnosed as SCA2, and two pedigrees were diagnosed as SCA2 pedigrees. Conclusions SCA2 can present as Parkinsonism, which is easily to be misdiagnosed as Parkinson's disease due to the effective treatment of levodopa. ENG is helpful in detecting subclinical cerebellar lesions. For Parkinsonism patients with family history, especially when cerebellar lesions are involved, ATXN2 gene testing is recommended.

Hepatocellular carcinoma (HCC) is the most common pathological type of primary liver cancer, with high fatality rate. The pathogenesis of HCC is complex, and the specific occurrence and development mechanism is still in the exploratory stage. CircRNA is a special endogenous noncoding RNA and mainly participates in the regulation of gene expression at the transcriptional and posttranscriptional levels. By regulating gene transcription, it acts as a molecular sponge of miRNA, participates in protein translation, and interacts with RNA binding protein (RBP). CircRNA is involved in the occurrence and development of HCC. Its abnormal expression in HCC cells is related to the pathological characteristics of HCC tissue, regulates the expression of downstream target genes, miRNA and proteins, participates in the proliferation, migration, invasion and apoptosis of HCC cells, and regulates tumor microenvironment and signal pathways, suggesting that circRNA may be a potential novel biomarker and therapeutic target for the diagnosis and prognosis of HCC. This paper reviews the biological mechanism of circRNA, its role in HCC, and research progress in diagnosis and treatment.

In order to achieve accurate prediction and optimization of milling surface residual stress. In this paper, Al7075-T6 aluminum alloy was taken as the research object. Firstly, a surface residual stress prediction model was proposed by analyzing the forces of orthogonal milling model and considering the machining parameters, milling force and heat. Compared with the traditional exponential empirical model, the superiority of the improved prediction model was proved. Taguchi algorithm and average signal-to-noise ratio were used to analyze the significant effect of process parameters on residual stress on workpiece surface, and the optimal process parameters for residual compressive stress generation were obtained. The optimized process parameters were verified by experiment and prediction model. The results show that compared with the traditional exponential model, the prediction accuracy of surface residual stress in X direction and Y direction is improved by 18.5% and 8.2%, respectively. After parameter optimization, the residual compressive stress in X and Y direction increases by 16.0% and 6.3%. The surface roughness value did not decrease obviously, which provides a theoretical basis for active control of residual stress of aerospace thin-walled parts.

Objective To summarize the key points of neuroendoscopic transcranial resection for cerebellopontine angle (CPA) tumors. Methods Twenty⁃eight patients with CPA tumors diagnosed and treated in Xiangya Hospital Central South University from January 2019 to December 2020 were included, and all of them underwent neuroendoscopic transcranial surgery to remove CPA tumors. Results All the 28 patients were successfully treated with neuroendoscopic transcranial resection of tumors in CPA region, and the tumors were totally removed. After operation, 9 cases of acoustic neuroma, 8 cases of meningioma, 9 cases of cholesteatoma, one case of hemangioblastoma, and one case of trigeminal neurinoma were confirmed by postoperative pathology. Postoperative facial nerve function was grade Ⅲ in 3 cases, grade Ⅱ in 6 cases, and grade Ⅰ in 19 cases. One patient had no obvious recovery of auditory nerve function injury, and 2 patients had dizziness, vertigo and nausea after operation. No complications such as cerebrospinal fluid leakage, meningitis and incision infection occurred, and no death occurred. At 3 months of follow ⁃ up, no tumor recurrence was seen on MRI, and no new neurological symptoms occurred. Conclusions Neuroendoscopic transcranial resection of CPA tumors has many advantages, but also has some shortcomings, and has a good development in the future.

Objective To investigate the effect and mechanism of orexin-A(OX-A) on the expression and function of glial glutamate transporter-1(GLT-1) and delayed neuronal death (DND) in hippocampus CA1 region of transient global cerebral ischemia(tGCI) in rats. Methods The rats were randomized into sham group, model group, OX-A group and OX-A+LY294002 group. The binding and glutamate uptake of GLT-1 in hippocampal CA1 region were detected by the [3H]-glutamate labeling method. The content of glutamate in hippocampal CA1 region was determined by the glutamate quantitative kit. The pathological changes of neurons in hippocampal CA1 region were observed by Nissol, NeuN and Fluro-Jade C staining. The expressions of GLT-1, p-PI3K and p-Akt in hippocampal CA1 region were detected by Western blot. Results OX-A promoted the binding and glutamate uptake of GLT-1, increased glutamate content, GLT-1 expression and PI3K/AKT signaling activity, and inhibited DND in the hippocampal CA1 region of tGCI rats (P＜0.05); However, LY294002 attenuated the above therapeutic effects of OX-A(P＜0.05). Conclusions OX-A can increase the expression and function of GLT-1 and reduce DND in the hippocampal CA1 region of tGCI rats by enhancing the activity of I3K/AKT signal.

The purpose of this study was to retrospectively investigate the prognostic significance of the lymphocyte-to-monocyte ratio (LMR) in patients with middle and advanced thoracic malignant tumors (esophageal and lung cancer) prior to and after radiotherapy. The clinical data of 74 patients with middle-advanced stage lung and esophageal cancer who received radiotherapy in Jiangsu Shengze hospital from January 2017 to December 2021 were screened for this retrospective analysis. Peripheral blood was collected, and LMR was recorded before and within one week following radiotherapy. The receiver operating characteristic curve (ROC) was established to obtain the best cut-off value for overall survival (OS). The correlation between LMR and prognosis before and after radiotherapy was examined. The Kaplan-Meier method was used to create the survival curve, and Cox proportional hazard regression model was used for univariate and multivariate analysis. The median follow-up time was 15 months, and the median age was 70 years. According to the ROC curve, the best critical value of LMR prior to radiotherapy was 2.46, AUC=0.719, while the best critical value of LMR after radiotherapy was 1.07, AUC=0.682. The Cox risk ratio model showed that clinical stage, diagnosis, combination therapy, and LMR before and after radiotherapy were all related to prognosis. Multivariate analysis revealed that clinical stage, combination therapy, and LMR before and after radiotherapy were independent prognostic factors in patients with middle and advanced lung and esophageal cancer following radiotherapy (p

BACKGROUND The Ordos Basin is one of the most important areas in China because it hosts lots of sandstone-type uranium deposits and a variety of other energy and mineral resources. The occurrence of uranium minerals is of great significance to the genetic understanding and prospecting of sandstone-type uranium deposits. However, the complex mineral composition, fine grain size, and various types and occurrences of uranium minerals make the initial identification stage of the study difficult. Currently, radiograph and electron probe microanalysis (EMPA) have played an important role in the identification of uranium minerals. Radiography can be used to obtain the position, occurrence and radioactive form of all uranium minerals in the light film at one time, but the mineral type cannot be identified. Moreover, it is a lengthy process and must be performed in darkness. Electron probe can be used to obtain the backscattered image of the uranium mineral. However, it takes time and effort to find uranium minerals with small content and small particle size in thin slices at high magnification, and it is impossible to quickly determine the types of associated minerals in BSE images. OBJECTIVES To find a more rapid and accurate method for identifying uranium minerals. METHODS The automatic mineral analysis system (AMICS), scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS) were used to identify minerals from sandstone-type uranium deposits in the north, south, and west margins of the Ordos Basin. RESULTS The minerals in the study were identified as coffinite, pitchblende, uraninite and uranophane. Pyrite and titanium oxide were closely related to uranium minerals, and other associated minerals were identified as quartz, rutile, feldspar, mica and kaolinite. CONCLUSIONS AMICS-SEM-EDS in situ analysis technology proves to be a reliable method for the rapid identification of uranium minerals, associated minerals, and their occurrences.

Objective To explore the expression of OPA interacting protein 5 (OIP5) in pancreatic cancer and its effect on the proliferation of PANC-1 cells. Methods The expression of OIP5 in pancreatic cancer and adjacent tissues was evaluated by database; the expression of OIP5 mRNA and protein in human pancreatic cancer cell lines like MIAPaCa-2, PANC-1, KP-3 and BxPC-3 was detected by real-time quantitative PCR (RT-qPCR) and Western blot, respectively; the expression of OIP5 gene silencing plasmid (pGCSIL-shOIP5)and control plasmid(pGCSIL-shCtrl)were observed, respectively infected with PANC-1 cells, then divided into OIP5 gene silencing group and shctrl control group. 5 days later, RT-qPCR and Western blot were used to detect slow virus knockdown efficiency, and flow cytometry was used to detect cell apoptosis. MTT and cell count were carried out in OIP5 gene silencing group and shctrl control group for 5 consecutive days. OIP5 gene silencing group and shctrl control group were incubated for 10 days to form colonies, then Giemsa staining was used to count total number of sets. Results The expression of OIP5 mRNA in pancreatic cancer was significantly higher than that in normal pancreatic tissue(P＜0.05), and the overall survival rate in patients with high expression of OIP5 was significantly lower than that in patients with low expression of OIP5 (P＜ 0.05), and the disease-free survival rate was also significantly lower (P＜0.05); the expression of OIP5 was higher in MIAPaCa-2, PANC-1 and KP-3, but lower in BXPC-3 cell line; the results of MTT showed that OIP5 was silenced in the 4th day. The proliferation rate of PANC-1 cells was significantly decreased on the 4th and the 5th day (P＜0.01); the number of cell colonies (9 on average) after OIP5 silencing was significantly lower than that in shctrl control group (40 on average) (P＜0.01); the apoptosis rate of PANC-1 cells after OIP5 silencing was 8.3% and 4.5% higher than that of shctrl (P＜0.01). Conclusions OIP5 is highly expressed in pancreatic cancer cell lines. OIP5 gene can regulate the proliferation, apoptosis and clonal formation of PANC-1 cells, suggesting that OIP5 may play a role as a oncogene in the pathogenesis of pancreatic cancer, thus providing a potential biomarker for targeted treatment of pancreatic cancer.

The quality changes of paddy during storage are greatly affected by external conditions. The external factors include temperature, moisture, air proportion, pests and microorganisms, etc., especially under the condition of high temperature of stored grain and high moisture of rice, mold of rice grows faster, which leads to the reduction of rice quality. This paper introduced the effects of storage conditions on paddy quality and the harmfulness of pests and harmful microorganisms to paddy storage. The research on existing paddy storage and new grain storage technologies were further discussed. The development of new technologies such as grain storage, the use of CO2 to detect rice mold, and the development of natural antifungal agents has provided more effective technical guarantees for the safe storage of paddy in our country. However, some new technologies have higher requirements on grain silos and it is difficult to realize nationwide popularization.

Objective To investigate the clinical features of cancer patients with venous thromboembolism (VTE) and related risk factors. Methods A retrospective case-control study was conducted to investigate the clinical and survival outcomes of cancer patients with VTE. Statistical analysis of VTE-related influencing factors was performed using chi-square test, Log rank test and proportional hazard regression model (Cox model). GraphPad Prism8.0 was used to draw the survival curve. Results We collected 11 620 patients with new-onset cancer, including 385 patients with VTE. The incidence of VTE in cancer patients was 3.31%. The incidence of VTE in malignant lymphoma patients was the highest (4.78%), followed by colorectal cancer (4.40%) and breast cancer (4.08%). Patients treated with combination therapy had a higher rate of VTE. TNM staging was positively correlated with the proportion of VTE in cancer patients. The median survival time was 17.90 months (95%CI: 10.21-25.59), and the 1-, 3- and 5-year survival rates were 51.89%, 37.76% and 18.88%, respectively. Multivariate analysis results showed that age, gender and TNM staging were independent risk factors affecting the survival and prognosis of patients. Conclusion Patients with malignant tumors are prone to VTE. High-risk patients should be closely observed to prevent VTE.

BACKGROUND Uranium mineral dating has been difficult to ascertain in mineralization chronology. With the development of in situ U-Pb isotope dating technology, it is possible to directly date ore minerals (uranium minerals). However, due to complex occurrences of sandstone-type U deposits, high requirements for laser ablation during in situ dating, and the lack of suitable external calibration standards, the dating accuracy needs to be improved. OBJECTIVES To solve the problem of shortage of reference materials for in situ U-Pb dating of sandstone-type uranium deposit. METHODS Two micro in situ U-Pb isotope dating methods are used for sandstone-type uranium deposits, attempting to solve the problem of no matrix-matched standards and improve the dating technology of sandstone-type uranium deposits. A laser ablation multi-collector inductively coupled plasma-mass spectrometer combined with an electron probe for micro-area in situ U-Pb isotope dating technology was established (LA-MC-ICP-MS & EMPA). RESULTS By optimizing the analytical technique, the Chenjiazhuang granite-type uranium deposit in Qinling was tested, and the ages were consistent with those determined by the isotope dilution-thermoionization mass spectrometry (ID-TIMS), which demonstrated the feasibility of non-matrix matched reference materials for isotope dating. This method was used to data Hongqinghe and Tarangalle sandstone-type uranium deposits in the Ordos Basin. At the same time, in situ U-Pb isotope dating of Hongqinghe and Ningdong sandstone-type uranium samples was carried out by fs-laser ablation multiple-collector inductively coupled plasma-mass spectrometry (fsLA-MC-ICP-MS). In situ U-Pb isotope ages were obtained for these two deposits, indicating that femtosecond laser ablation technology had a good application prospect in the dating of sandstone-type uranium deposits. CONCLUSIONS Combined LA-MC-ICP-MS and EMPA method can be selected for analysis of simple-texture and relatively old minerals, whereas fsLA-MC-ICP-MS method is recommended for samples requiring high spatial resolution.

2019 coronavirus disease (COVID-19) is an infectious disease caused by SARS-COV-2 virus, and lacks specifically effective treatment till now. Its clinical manifestations and the concerning studies at home and abroad have shown that viral sepsis caused by SARS-COV-2 virus has typical characteristics of pathophysiology of sepsis, that is, early cytokine storm and subsequent immunosuppression stage. At present, although great attention has been paid to the treatment and clinical research on cytokine storm, the later stage of immunosuppression is neglected. Patients' lower immunity, secondary infection and multiple organ failure are quite common, and all these lead to rapid deterioration and even death. Therefore, it is of great significance to pay attention to the investigation and treatment of sepsis immunosuppression stage caused by COVID-19, and to study the drugs with bidirectional immune regulation such as artesunate so as order to reduce the mortality of patients with severe or critical COVID-19. What's more, we strongly recommend that chloroquine phosphate should be used with great caution for severe and critical patients.

Objective To analyze the characteristics of laboratory biomarkers associated with hypercoagulability in patients undergoing lung cancer surgery during the perioperative period and its diagnostic value for deep venous thrombosis (DVT). Methods We enrolled 143 patients treated with lung cancer surgery based on eligibility criteria. The lower extremity deep vein was examined perioperatively by compressed ultrasonic (CUS) to determine the DVT. The laboratory biomarkers associated with hypercoagulability were measured. We analyzed the area under curve (AUC) of ROC and cutoff in DVT (+) and DVT (-) groups with significant different biomarkers. Results Thirty-three patients with DVT were first diagnosed from the postoperative 3rd day (POD3) to 7th day (POD7). The fibrinogen degradation product (FDP), D-Dimer, brain natriuretic peptide and C reactive protein were significantly increased in the postoperative POD1-POD7 (P < 0.05). There were significant differences in the change levels of FDP and D-D during the perioperative period between the DVT (+) and DVT (-) groups (P < 0.05). The AUC and diagnostic cutoff values of POD1-FDP, POD3-FDP, POD3-D-D were 0.726 and 4.65μg/ml, 0.811 and 4.85 μg/ml, 0.772 and 1.55 μg/ml, respectively. The sensitivity and specificity of serial test were 46.5% and 94.9% for the combined diagnosis. Conclusion The value of increased levels of POD1-FDP, POD3-FDP and POD3-D-Dimer for DVT diagnosis is moderate. The combined diagnosis with serial test could improve the specificity of diagnosis.

MnO2@Ni(OH)2 core/shell structure nanowire arrays (NWAs) supported on carbon cloth were successfully synthesized by a two-step method, applied in flexible all-solid-state asymmetric supercapacitors (ASCs). The Ni(OH)2 nanosheets wrapped on the surface of each MnO2 nanowire uniformly, which can increase the capacitance of MnO2 NWAs to a high specific capacitance of 432.8F/g at 5mV/s. The electrode also exhibits good cycling ability, 92.3% of the initial capacity can be remained after 2000 cycles at 5A/g. The assembled MnO2@Ni(OH)2//MnO2 asymmetric device with a maximum voltage of 1.8V has been fabricated, delivering both high energy density (69.2Wh/kg) and power density (4.5kW/kg at 54.6Wh/kg). These results show that MnO2@Ni(OH)2 NWAs with large specific surface area, combined with the flexible carbon cloth substrate can be applied in supercapacitor field in large scale.

The W-Mo-Cu alloy was rapidly prepared by a large current electric field sintering method at 875-1000℃. Effect of sintering temperature on microstructure, hardness and electrical conductivity was investigated. Based on the dimensional change during alloy sintering, the sintering characteristic index was obtained by fitting calculation, and the main migration mechanism of W-Mo-Cu alloy during sintering was inferred. The results show that the pores of the W-Mo-Cu alloy decrease, the relative density, microhardness and electrical conductivity increase at the same time with the increase of sintering temperature at 875-975℃. When the sintering temperature is between 875℃ and 925℃, the densification of the alloy is mainly caused by plastic deformation instead of sintering; when the sintering temperature is higher than 925℃, the order of the main migration mechanism experienced during the densification of W-Mo-Cu alloy is plastic flow, volume diffusion, grain boundary diffusion and surface diffusion.

The noise orthogonal test of the automotive synchronous belt transmission system is carried out based on two wheels of unloaded test method. The influence law of the three parameters of the active wheel speed,synchronous belt bandwidth and tensioning force on the synchronous belt transmission noise is analyzed systematically. The orthogonal table is extended by regression,without any increase in testing time,a regression mathematical model is established based on the objective function of automobile synchronous belt transmission,and the accuracy of the mathematical model is verified by experiment. A method for the research of the influence factors of transmission noise of automobile synchronous belt is provided,and the prediction of transmission noise of automobile synchronous belt is realized.

Due to long-term used in the harsh environment,the functional degradation of the FPSO crane such as corrosion,fatigue crack,local failure occurred. The functional degradation could affect the normal use of the FPSO crane. According to the actual surveying data,the finite element model of the FPSO crane has been established by finite element software Ansys. The stress of high stress area has been tested according to the stress distribution of the FPSO crane. The calculation results of Ansys have been compared with the test data. The errors between calculation results and test data are in 14%,except of point 1941.The stress value of the most dangerous area is 247. 96 MPa,which is less than the allowable stress of the crane. Thus the strength requirement is satisfied. The relation between stress of key area of crane and the angle β of lifting has been analyzed. When the weight of lifting is close to the maximum working load,the working condition of β > 15°is preferred.

For common non-destructive measurement methods of anchor stock have problem that vibration wave ca't couple well with the anchor stock, the paper proposed a new non-destructive measurement method of anchor stock legth based on electricity standing wave. The method is based on equivalent principle between transmission line and antenna, and uses standing wave theory of transmission line to measure length of metal anchor stock, that is, scan input signal frequency, when the first minimum voltage of the anchor stock appears, the corresponding signal wavelength is 4 times of anchor stock length, and the anchor stock length can be calculated. The measured result shows that the method has convenient coupling and good measurement result.

To analyze the research status and development trend of ionic liquids toxicology, VOSviewer and HistCite software were used to visually analyze the countries, institutions, authors, keywords, etc. of 1 178 ionic liquids toxicology related to literatures in the Web of Science core database from 2000 to 2021. The results show that: the number of literatures in the field of ionic liquids toxicology is increasing year by year, and China is far ahead of other countries in both the number of papers published and the total citation score. At present, the paper is mainly based on team cooperation within institutions, with strong independence. Although Chinese institutions and authors rank higher in the number of papers published, their total local citation score is not outstanding. The main focus of research in ionic liquids toxicology is imidazolium. The research focuses on the evaluation of lethality and composition changes in organisms, and the construction of structure-activity relationship models between ionic liquids and organisms. The mechanism of ionic liquids toxicity and combined pollution may be the focus of future research. [ABSTRACT FROM AUTHOR]

Under the background of systematic and full-field promotion of sponge city construction, the allocation of Low Impact Development (LID) facilities holds significant implications for urban stormwater management. In this study, we established a stormwater model for he Yixing City area in Jiangsu Province, utilizing the Storm Water Management Model (SWMM) . A comprehensive objective function was ormulated, taking into account the reduction rates of runoff volume, Suspended Solids (SS) load, Life Cycle Cost (LCC), and the area occupied by LID facilities. Through the assignment of weights to objective parameters in different scenarios, the multi-objective optimization problem was transformed into a single objective optimization problem, where each weight represented the priority of the corresponding objecive parameter. The Genetic Algorithm (GA) was then applied to derive the optimal LID layout under diverse scenarios, to analyze and compare the impact of different priorities and recurrence periods of target parameters on optimization results. The results indicate that: (1) Under he same weight conditions, LCC exhibits a more pronounced impact on GA optimization calculations compared to LID area. (2) The spatial alocative optimization of LID facilities in small subcatchments located upstream is particularly susceptible to the rainfall return period. The research findings offer valuable insights for the spatial allocative optimization of LID facility and urban flood control in sponge city construction. [ABSTRACT FROM AUTHOR]

In order to improve the efficiency of artificial breeding and aquaculture efficiency of Chinese horseshoe crab, the breeding equipment used in the process of artificial breeding and larval cultivation of Chinese horseshoe crab was optimized and improved, and the breeding effects of the old and new equipments were compared. The results showed that the average egg-production amount per female Chinese horseshoe crab in the new temporarily rearing system of Chinese horseshoe crab was 3530, which was significantly higher than that in the conventional temporarily rearing system (2320) (P<0.05), and the cleaning frequency of the filter vat was significantly lower than that in the conventional system (P<0.05). The ratios of 2nd instar larvae (93.33%) and 3rd instar larvae (35.33%) obtained by the new larval rearing device were significantly higher than those obtained by the conventional larval rearing device (being 83.33% at 2nd instar and 10.33% at 3rd instar) (P<0.05). The average mortality rates of 1st instar larvae (6.67%) and 2nd instar larvae (6.33%) in the new larval rearing device were significantly lower than those in the conventional larval rearing device (16.67% and 30.67%) (P<0.05). The 2nd instar molting began from the 11th to 12th day and the 3rd instar molting began from the 21st to 30th day in the new larval rearing device, while the 2nd and 3rd instar molting began from the 31st to 40th day in the conventional larval rearing device. It could be seen that the molting time required for the new larval rearing device was significantly reduced (P<0.05). The above results showed that the new temporarily rearing system of horseshoe crab could enhance the purification capacity of waters, increase the egg laying amount of horseshoe crab, significantly accelerate the molting speed of the 2nd instar and 3rd instar juvenile Chinese horseshoe crab, and reduce the mortality rate of the 2nd instar juvenile Chinese horseshoe crab. The research results could provide important technical reference for the subsequent large-scale artificial breeding and larval cultivation of horseshoe crab. [ABSTRACT FROM AUTHOR]

In order to explore the toxic effect and safe concentration of potassium permanganate, povidone iodine, chlorine dioxide and polyhexamethylene guanidine on Marphysa sanguinea, the acute toxicity test of these four disin¬fectants on M. sanguinea was carried out by using the semi-hydrostatic test at room temperature. The results showed that the tolerance capability of M. sanguinea to the four kinds of disinfectants was 10% povidone iodine> potassium permanganate>10% chlorine dioxide>25% polyhexamethylene guanidine. The 24 h, 48 h and 96 h median lethal concentrations of 10% povidone iodine were 418. 41, 242. 67 and 89. 49 mg • L-1, respectively, and the safe concentration was 8. 95 mg • L-1. The 24 h, 48 h and 96 h median lethal concentrations of potassium permanganate were 14. 10, 9. 59 and 7. 38 mg •L-1, respectively, and the safe concentration was 0. 74 mg • L-1. The 24 h, 48 h and 96 h median lethal concentrations of 10% chlorine dioxide were 89. 26, 47. 18 and 4. 61 mg • L-1, respectively, and the safe concentration was 0. 46 mg * L-1. The 24 h, 48 h and 96 h median lethal concentrations of 25% polyhexamethyl- ene guanidine were 11. 44, 3. 08 and 1. 13 mg • L-1, respectively, and the safe concentration was 0. 11 mg • L-1. The use of povidone iodine and chlorine dioxide was suitable in the breeding and culture production of M. sanguinea, while polyhexamethylene guanidine and potassium permanganate should be used with caution. [ABSTRACT FROM AUTHOR]

The bidirectional DC-DC converter was selected as the research object. Based on the connection topology of energy storage and consumption components in the hybrid energy storage system, power demand indicators were determined for different energy flow directions, and the parameters of the main converter components and appropriate circuit components were established. A circuit model of buck/boost was constructed, and an adaptive fast terminal sliding mode control strategy was designed. The controller parameters were optimized using an improved genetic algorithm. Different control parameters have different control effects. In order to obtain better voltage output characteristics,the parameters of the adaptive fast terminal sliding mode control strategy were optimized.The proposed method can be verified to exhibit good control performance through simulation.

Abstract [Objective] Rosa odorata is an important germplasm resource of Rosa, which has high value of appreciation and utilization. In order to promote the development and utilization of the germplasm resources, the chromosome ploidy, floral synthesis, and characteristics of breeding systems of different R. odorata were studied. [Methods] The chromosome ploidy, pollen vitality, stigma vitality, and flower characteristics of 9 R. odorata were observed and determined by flow cytometer and outcrossing analysis. We also analyzed the breeding systems. [Results] (1) 9 R. odorata were identified as diploid, and their flowers were bisexual with bright colors and mostly double or semi-double petals. Male and female flowers of 5 varieties were matured at different time. (2) 9 R. odorata pollen vitalities were low, with the highest vitality of 35.47%. The outcrossing index (OCI) of all varieties was ≥4, and pollen volume-ovule ratio (P/O) values ranged from 6 709 to 75 521. [Conclusion] The breeding system of 9 R. odorata is mainly outcrossing, and the breeding system is affected by many factors, such as the number of pollen grains and petals.

[Objectives] To predict the future range of the Fall Webworm (Hyphantria cunea) in China in order to improve the prevention and control of this pest. [Methods] Records of the Fall Webworm collected in China from 2003 to 2020 were obtained and organized, and 19 bioclimatic variables for that period were calculated based on the monthly maximum and minimum temperatures and monthly precipitation. DEM data were obtained and future climate variables under different climate scenarios were calculated. The nine most important environmental variables were identified by VIF and Spearman's rank correlation. Suitable current, and future, habitat for the Fall Webworm were then predicted based on an integrated Biomod2 model. [Results] Suitable habitat for the Fall Webworm is currently concentrated in Liaoning, Shandong, Hebei, Anhui and other provinces. The range of the Fall Webworm is predicted to increase under different climate scenarios, with the overall trend being an expansion of the species' range into northeastern and southern China. [Conclusion] The results provide early warning of areas likely to be colonized by the Fall Webworm, allowing appropriate control measures to be implemented beforehand to prevent its spread. [ABSTRACT FROM AUTHOR]

The purpose of the experiment was to study the feeding value of micro storage and ammoniated corn stalks on Liaoning Cashmere goats. A total of 24 Liaoning Cashmere goats with an average weight of (24.14±1.02) kg were selected and randomly divided into three groups, with eight replicates in each group and one sheep in each replicate. Group I was control group, the roughage was corn stalks+peanut stalks+alfalfa hay, group II was micro storage corn stalks+peanut stalks+alfalfa hay, and group III was ammoniated corn stalks+peanut stalks+alfalfa hay. The formal trial period was 30 d, during which the metabolic test was conducted for 7 d. The results showed that the CP content of corn straw after micro storage and ammoniation treatment increased by 14.84% and 93.70% respectively, and the CP content of ammoniated straw was significantly higher than that of the other groups (P<0.05). The average daily gain of test sheep in group II and group III was significantly higher than that in control group (P<0.05), and the feed to gain ratio was significantly lower than that in control group (P<0.05). The apparent digestibility of CF, NDF and ADF in control group was significantly lower than that in test group (P<0.05). The study indicates that the micro storage and ammoniation can increase the content of CP, reduce the content of corn straw fiber, improve its quality, increase the digestibility of nutrients, improve the growth performance, and increase economic benefits. The micro storage treatment of corn straw has the best effect. [ABSTRACT FROM AUTHOR]

Objective To understand the role of interleukin-24 (IL-24) in the liver regeneration after partial hepatectomy (PHx).Methods The rats were injected intradermally with mIL-24-Fc or phosphate buffered saline (PBS).The proliferating cell nuclear antigen (PCNA) staining was used to detect the liver regeneration. To assess the liver injury,the serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels were determined at multiple time points.The qPCR was used to detect the levels of IL-24 and IL-20R2 mRNA.ELISA and western blotting were used to detect the expressions of serum IL-24 protein, phosphorylated signal transducer and activator of transcription 3 (p-STAT3) protein in serum of rats in each group.Results The results of HE staining showed that the intradermal injection of mIL-24-Fc fusion protein into the skin of rats induced the keratinocyte proliferation.The liver regeneration was significantly increased at 12 h and 48 h after PHx.The concentrations of AST and ALT at 3 h,12 h and 48 h after PHx were far higher. The levels of IL-24 mRNA were slightly decreased slightly at 3 h in the PHx group and the sham-operated groups (the laparotomy process were the same as the PHx group,but the liver was intact).The IL-24 mRNA levels in the sham-operated group and the control group were compared at 12 h.However,the IL-24 mRNA level in the PHx group was higher than that in the control and sham-operated groups at 12 h.The higher level of IL-24 mRNA was found at 48 h after surgery in the PHx group.The gene transcription of IL-20R2 were induced at 3 h,12 h and 48 h in PHx group.However,the induction of IL-20R2 gene transcription at 3 h,12 h, and 48 h in the sham-operated group were lower than those in the control group at 3 h,12 h and 48 h.The serum levels of IL-24 in both PHx group and sham-operated groups were significantly increased,peaked at 12 h,and then began to decline.The p-STAT3 was still activated for the first 3 h.And the p-STAT3 continued to increase for 24 h after PHx,which was consistent with the serum levels of IL-24.Conclusion The up-regulated of serum IL-24 is the result of stress reaction after the PHx.IL-24 in tissues stimulates liver regeneration after hepatectomy via an unknown cell-surface-receptors signaling pathway or non-receptor signaling pathway. [ABSTRACT FROM AUTHOR]

Enriched tungsten-186 oxide is one of the stable isotope products of tungsten. It is used to prepare the radioactive medical isotope product 188WO3, which can be used as the target material of 188W/188Re generator to produce medical 188Re isotope product, so as to meet the increasing requirements of biomedical markers, bone cancer treatment and other medical related fields. In order to satisfy the current market demand for 186WO3, the preparation process of 186WO3 with tungsten-186 hexafluoride (186WF6) as the raw material was studied and determined. The key parameters of preparation processes were systematically optimized, and the influence of essential parameters on the 186WO3 product purity and process yield was obtained, which determined the optimal process parameters. Finally, the high conversion rate process route was achieved, and the enriched 186WO3 products were obtained. The chemical purity of 186WO3 can reach 99.91%-99.93% with the process yield of 89.8%-90.5% under the optimal process conditions. The research consequences of this article can provide a theoretical and experimental basis for the batch preparation of 186WO3, and give the technical reference for the process of preparing metal oxides from other similar gas-phase fluorides.

Slow water bodies, mainly represented by landscape lakes and ecological wetlands, are becoming more and more important in urban construction. How to increase water exchange areas and slow down the deterioration of water quality is an important problem we are faced with. Based on particle image velocimetry (PIV) technique, the effects of spacing ratio, diversion flow rate and shape of double bluff body (islands) on two-dimensional flow field distribution, water displacement rate and average velocity of the flow field in slow water are studied. The experimental results show that the diversion flow rate is the main controlling factor for the intensity of the flow field in the slow water, and the hydrodynamic exchange efficiency and the average velocity of the water flow are proportional to the diversion flow. The bluff body can obviously improve the range of flow movement and increase the fluidity of water. It is found that the fluidity of the flume water is the strongest and the displacement rate is the highest when the prism and cylinder are disturbed together. The maximum displacement rate reaches 72.89% when Q=6.8 L/min and G/D=6. The experimental results will provide some reference for optimizing the layout of islands in the landscape lake. [ABSTRACT FROM AUTHOR]

Objective·To explore the association of somatizatic symptoms, anxiety and depression with clinical prognosis in the patients with chronic heart failure (CHF).Methods·The patients with CHF who visited the Department of Cardiology, Renji Hospital, Shanghai Jiao Tong University School of Medicine from January 2018 to December 2021 were included. Demographic data and clinical features of the patients were collected. The Self-reported Somatic Symptom Scale of China (SSS-CN), the Patient Health Questionnaire-9 (PHQ-9), and the Generalized Anxiety Disorder-7 (GAD-7) were used to evaluate the patients′ conditions. Telephone follow-up was conducted at the 12th month after the first visit, and the specific information of the patients′ end-point events (including death, re-hospitalization, causes of death and re-hospitalization) was collected. Survival curve and Cox regression analysis were used to evaluate the clinical prognosis of the patients.Results·A total of 195 patients were included. The SSS-CN scores in CHF patients were different between the two genders, among the different heart rate groups and the different cardiac function grades of New York Heart Association (NYHA), also between the patients with anxiety/depression or not (all P

Bacterial extracellular vesicles (BEVs), bacteria-derived extracellular vesicles, are key intermediates of bacteria-bacteria and bacteria-host interactions. They are a 'double-edged sword'. On one hand, they play a negative role in the onset and progression of various diseases, including intestinal inflammatory disease, neurological disease, liver disease, metabolic disease, autoimmune disease and cancer. On the other hand, they play a positive role as potential biomarkers, vaccines, antitumor agents. The application of BEVs may provide novel strategies for the diagnosis and treatment of diseases. However, research on BEVs is still in its infancy, and challenges remain in vesicle isolation techniques, disease diagnostic specificity, optimization of vesicle storage and transport. Thus rigorously designed clinical studies are required to verify their diagnostic and therapeutic value.

Objective By analyzing the mortality and related factors of HIV / AIDS patients in Huangshi city from 2000 to 2019, we explore the methods of reducing the mortality. Methods Downloading the relevant cards from China's AIDS Prevention and Control Comprehensive System, we analyze the database using excel 2007 and Spass21.0. Results Among the 2 361 HIV /AIDS cases, 760 cases died, the case fatality rate was 32.19%. 600 patients (78.95%) were over 50 years old at the time of death; 73.15% (556/760) patients had received CD4+ T lymphocyte test, of which 65.11% (362/556) patients had detection value<200/µL; 50.79% (386/760) patients had received antiviral therapy; 54.61% (415/760) patients died within one year after diagnosis. Multivariate binomial Logistic regression analysis showed that other patients (OR=5.081, 95%CI=2.776- 9.302), the patients who did not receive antiviral therapy (OR=4.716, 95%CI=3.002-7.407), the CD4+T lymphocyte value <200 /µL (OR=8.550, 95%CI=4.289-17.046) and the patients who did not detect lymphocyte (OR=29.392, 95%CI=13.466-64.155) were more likely to die within one year. The proportion of AIDS related death causes decreased year by year, reaching 39.13% in 2017. The average age of patients at the time of death increased from 37.39 years in 2001 to 66.86 years in 2019. Conclusion The cumulative mortality rate of HIV/AIDS in Huangshi City is higher than the national average, the average age at the time of death was higher than the national average, and the trend is increasing year by year. Late onset cases are more likely to die within one year after diagnosis. The coverage rate of antiretroviral treatment is increasing, and the proportion of non AIDS related deaths and the survival time of patients are increasing. We should continue to carry out early detection and treatment, improve the effect of antiviral therapy, prolong the survival time of patients and reduce the mortality. [ABSTRACT FROM AUTHOR]

BackgroundNeutral beam injection heating is a very effective auxiliary heating method in magnetic confinement fusion experiment. The built 5 MW neutral beam injection plays an important role in the H-mode operation with 1 MA plasma current of HL-3 tokamak. In order to achieve the H-mode operation with 2.5 MA, three neutral beam injection heating systems will be constructed with a total heating power of 20 MW.PurposeThis study aims to investigate the transmission efficiency of 7 MW neutral beam injector designed for HL-3 tokamak.MethodsThe beam transmission efficiency was studied from two aspects of neutralization efficiency and power deposition. The initial target thickness of the neutralizer under two beam parameters was analyzed based on Monte Carlo method. The optimal neutralization efficiency was achieved by adding neutralizer gas supply, and the target thickness required for the optimal neutralization efficiency was given. Finally, power deposition method was employed to analyze the transmission performance of the two neutral beams with different parameters at different beam divergence angles.ResultsThe results show that the optimal neutralizer efficiency is achieved when the neutralizer gas supply is 0.6 Pa∙m3∙s-1 and 1.1 Pa∙m3∙s-1, respectively. The neutral beam power obtained under two beam parameters with a divergence angle of 1.2° is 7.2 MW and 6.8 MW, respectively, and the corresponding transmission efficiency is 0.4 and 0.35, respectively.ConclusionsThe new neutral beam injector of HL-3 tokamak will be built according to the design of this study, with optimized parameter of 100 kV/45 A, while still retaining the ability to deliver a 120 keV deuterium beam.

Objective To study the clinical application of the modified nutritional risk screening tool and nutrition assessment in pediatric patients in China, and to provide a theoretical basis for establishing a standardized nutritional management process for pediatric patients. Methods A retrospective analysis was performed for the nutritional risk screening and nutrition assessment data of 16 249 hospitalized children. According to the degree of nutritional risk, the children were divided into a high nutritional risk group with 588 children, a moderate nutritional risk group with 4 330 children, and a non-nutritional risk group with 11 331 children. Nutrition assessment results were compared between groups. The composition of nutritional risk screening scores and the impact of nutritional risk screening on the rate of nutrition support therapy were analyzed. Results The incidence rate of nutritional risk was 30.27% (4 918/16 249), and the incidence rates of malnutrition and overnutrition were 27.37% (4 448/16 249) and 11.29% (1 834/16 249), respectively. Nutrition assessment results were significantly correlated with nutritional risk (≥5 years old: rs=0.313, P<0.05; <5 years old: rs=-0.304, P<0.05). There was a significant difference in the composition of scoring items between the groups with different nutritional risks (P<0.05). With the implementation of nutritional risk screening, there was a gradual increase in the rate of nutrition support therapy year by year (P<0.05). Conclusions There is a high incidence rate of nutritional risk in hospitalized children. The use of the modified pediatric nutritional risk screening tool can promote the implementation of standardized nutritional management. [ABSTRACT FROM AUTHOR]

Objective: Accurate identification and location of paper packaging box defects. Methods: The improved network model of Faster R-CNN was applied to automatically detect box defects. The data of the training set picture was enhanced and noise was added to improve the training accuracy and robustness of the model. The feature extraction network was replaced with ResNet50, and the feature pyramid network （FPN） was fused to improve the multi-scale detection ability of the model. K-means++ was used to cluster the defect scale in the dataset and optimize the anchor box scheme. Results: The average accuracy （AP） of the improved Faster R-CNN model on the test set reached 93.9%, and the detection speed reached 8.65 f/s. Conclusion: The improved Faster R-CNN model can effectively detect and locate box defects, which can be applied to the automatic detection and sorting of box defects.

The present switching speed measurement devices for circuit breakers are mostly low in accuracy， severe on installation conditions， and cannot achieve real-time online monitoring. A new system for real-time switching speed measurement of circuit breakers based on a line array camera is proposed. First， the experimental technique is studied by building an experimental frame simulating the rotation of circuit breakers. A turntable is installed at the output of the motor to simulate the rotating shaft of the circuit breaker actuator， and a line array camera is placed on the turntable side to capture the black and white narrow strip images of the targets set on the turntable. Afterward， the appropriate resolution， exposure time and judgment threshold are chosen to analyze the changes of the images taken by the line array camera in real time to obtain the real-time motion travel of the target and the rotation speed of the turntable. The switching speed of the circuit breaker is obtained to verify the feasibility of the measurement scheme. Finally， it is applied to a pilot substation to monitor the motion travel of the switching of the circuit breaker in real time. Compared with the existing technology， this measurement system can process the images more quickly and measure the switching speed more precisely and stably.

In this study, TtsI, a regulator of HH103 type III secretion system in Sinorhizobium was detected. The mutant HH103ΩTtsI was obtained by three-parental hybridization and identified by PCR. Type III effector expression analysis showed that TtsI mutation could inhibit the transcription and expression of effector genes such as NopAA, NopD, NopL, and NopP in the presence of genistein. When TtsI mutated, it could significantly inhibit the synthesis of rhizobium HH103 type III effector. TtsI mutants and wild-type rhizobium HH103 were used to identify nodules in 10 soybean germplasm of different ecological regions. These results showed that the TtsI mutation could significantly reduce the average number of nodule and the dry weight of nodule. The nodule phenotype identification results of the introduced line population showed that the TtsI mutant significantly reduced the average nodule number of the introduced line. This showed that TtsI played an important role in the establishment of a soybean-rhizobia symbiotic system depending on other type III effectors. [ABSTRACT FROM AUTHOR]

Osteoporosis affects elderly people's quality of life, which has become great concern of the society as fastest-growing aging population. This paper analyzed the potential reasons of osteoporosis in the elderly from the perspective of Traditional Chinese Medicine (TCM), and reviewed the studies about TCM dietary therapy, exercise guidance, emotional care in elderly patients with osteoporosis, in order to provide reference for promoting healthy lifestyle and reliving clinical symptoms in elderly patients with osteoporosis. (骨质疏松症严重影响老年人生活质量, 随着人口老龄化趋势加重, 已然成为社会关注的焦点。本文从中医角度分析老年骨质疏松症的病因, 总结中医食疗、运动指导和情志护理等措施在老年骨质疏松症患者中的应用现状, 旨在为促进患者养成健康的生活方式、缓解疾病症状提供参考。)

BACKGROUND Rutile, anatase and brookite are TiO2 polymorphs. In the rutile in-situ U-Pb dating, rutile was determined primarily on electron microprobe data and cathodoluminescence images, while these methods sometimes could not effectively distinguish rutile, anatase and brookite. If there are a small number of the TiO2 polymorphs in the sample, the obtained age may be a mixed age with no significant geological significance. Therefore, the identification of TiO2 polymorphs is very important. OBJECTIVES To identify TiO2 polymorphs from the bauxite deposit in central Guangxi. METHODS TiO2 minerals in bauxite from central Guangxi were used as the research object, and laser Raman spectroscopy was applied to the identification of TiO2 isomorphic minerals. RESULTS Four groups of laser Raman spectral lines with different characteristics were identified. The first three groups have laser Raman spectral peaks of anatase, rutile and brookite, respectively. The fourth group has two kinds of laser Raman spectral characteristic lines, one has both anatase characteristic peaks of 144, 198, 397, 513, 636cm-1 and rutile characteristic peaks of 442, 607cm-1, the other has both anatase characteristic peak of 144cm-1 and brookite characteristic peak of 153, 247, 325, 636cm-1. CONCLUSIONS The TiO2 polymorphs of the bauxite deposit includs rutile, anatase, brookite and intermediate minerals that undergo phase transformation. The study indicates that the bauxite deposit in central Guangxi may have undergone a later regional metamorphism, leading to the transformation of TiO2 polymorphs. The laser Raman spectroscopy provides a new method for the identification of TiO2 polymorphs andrutile for in-situ U-Pb dating.

ObjectiveTo observe the effect of respiratory training with Liuzijue on pulmonary function, activities of daily living and quality of life in patients with ischemic stroke and hemiplegia.MethodsA total of 76 patients with ischemic stroke and hemiplegia who were hospitalized in the Second Affiliated Hospital of Fujian Medical University and the Jinjiang Hospital of Traditional Chinese Medicine Affiliated to Fujian University of Traditional Chinese Medicine were recruited from March to September 2019. The patients were divided into control group (n=38) and experimental group (n=38) using a random number table method and according to the order of admission. The control group received routine treatment, nursing care, and early rehabilitation training; in addition to these interventions, the experimental group received Liuzijue respiratory training, and the patients were instructed with nasal inspiration and mouth expiration method, and the expiration was carried out in accordance with the Liuzijue sequence of "xu" "he" "hu" "si" "chui" "xi" 30 min/time, once a day, 5 days a week, lasting for 12 weeks. The forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), peak expiratory flow (PEF) rate and maximum voluntary ventilation (MVV) volume of the two groups were evaluated by portable pulmonary function meter before and after the intervention; the modified Barthel index (MBI) was used to evaluate activities of daily living; the stroke impact scale (SIS) was used to evaluate the quality of life.ResultsBefore the intervention, there was no statistical difference in the FVC, FEV1, PEF, MVV, MBI, and SIS scores between the two groups (P>0.05). After 12 weeks of intervention, the scores of the FVC, FEV1, PEF, MVV, MBI, SIS in both groups improved significantly compared with those before intervention, and the differences were statistically significant (P1, PEF, MVV of the experimental group were (2.63±0.68) L, (2.33±0.68) L, (4.49±1.39) L/s, (44.08±13.89) L/min respectively, which were better than those in the control group (2.31±0.50) L, (2.03±0.48) L, (3.79±1.46) L/s, (34.74±15.21) L/min, and the differences were statistically significant (PPP

BACKGROUND In recent years, the in situ Hf isotopic determination method of zircon has been widely used in isotopic geochemistry, and has become an important method to explore the genesis of rocks, the source of ore-forming materials and the evolution of crust and mantle. However, for some rocks, the lack of zircon seriously hinders the restriction of formation and evolution. The development of Hf isotopic determination methods for oxide-type U-bearing accessory minerals, such as rutile, cassiterite and columbite is urgently needed. OBJECTIVES In order to accelerate the studies of in situ Hf isotopic determination of oxide-type U-bearing minerals and their application to the geological research. METHODS In situ Hf isotopic analysis of oxide-type U-bearing accessory minerals was reviewed with NEPTUNE multiple-collector inductively coupled plasma-mass spectrometry (MC-ICPMS) and a 193nm excimer laser ablation system. RESULTS Combined with relevant research work in recent years, the development history of Lu-Hf isotope analysis technology for accessory minerals was briefly described, and the latest progress and existing problems in in-situ Hf isotope determination methods for oxide-type uranium-bearing minerals such as rutile, cassiterite and niobite were systematically reviewed. The current technical difficulties such as the correction strategy for isobaric interference, the lack of quality control standard samples, the lower Hf content, and the improvement of analytical sensitivity were discussed in detail. CONCLUSIONS The low Hf content of oxide-type U-bearing accessory minerals requires a larger spot diameter. The femtosecond laser has the characteristics of fine and uniform grain size of the ablation samples. The combination of femtosecond laser and MC-ICPMS (fs-LA-MC-ICPMS) can reduce the spot diameter and improve the spatial resolution, which is the development direction of in situ Hf isotope analysis of oxide-type U-bearing accessory minerals in the future.